Friday September 18th
In Notebook:
What experience do you have using a
microscope?
What are some practical uses of a
microscope that you are aware of?
Today’s Learning Targets
Agenda:
1. Microscope Notes
2. Microscope Lab
DUE: Tuesday Sept. 22nd
What’s my power?
To calculate the power of magnification, multiply the power of the ocular lens by the power of
the objective.
Eyepiece is 10x and
objective is 10x what is
total magnificaion?
Basic Rules and Tips
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Carry using arm and base
Flat Surface
Adjust light using diaphragm
Use stage clips
Focus with course adjustment on low and
medium power only!
– High Power use fine adjustment only
How to make a wet-mount slide …
1 – Get a clean slide and coverslip from your teacher.
2 – Place ONE drop of water in the middle of the slide. Don’t use too much or the water will run
off the edge and make a mess!
3 – Place the edge of the cover slip on one side of the water drop.
4 - Slowly lower the cover slip on top of the drop.
Cover Slip
Lower slowly
Monday September 21st
In Notebook:
How do you calculate total
magnification of an object through a
microscope?
Today’s Learning Targets
Agenda:
1.Microscope Notes
2.Microscope Measuring Lab
DUE: ?
3.Friday’s Microscope Lab due TODAY at end of hour
4.REMINDER: Lab report due TOMORROW! (Do not need to print)
5.REMINDER: Quiz TOMORROW and test on THURSDAY!
Calculate Field of View
1. Clear ruler
2. Determine how many
millimeters across the field.
(1 millimeter is the distance
from the middle of one mark
mark to the middle of the next
mark)
3. Convert to micrometers
(1mm=1000μm)
Examples:
1. If it’s 1.5 mm across, then the field of view is:
2. If it’s 2.4 mm across, then the field of view is:
DOES THE FIELD OF VIEW SIZE CHANGE WITH
DIFFERENT MAGNIFICATIONS?
• Low power = 4 mm or 4000 μm
• Medium power = 1.5 mm or 1500 μm
• High power = .5 mm or 500 μm
Calculate Size of Specimen
1. Figure out how many will fit across the field of
view (estimate)
2. Divide field of view length by how many fit
across
Example: If 7 protozoa fit across my field of view
with a length of 1400μm, then what is the size of
one protozoa?
Practice
Looking through my low power objective I see 4 mm on the
ruler. Later I was looking at my hair and I estimate I could fit
10 hairs side by side in my field of view. How wide is my
hair?