This week’s objectives:
•To name the parts of the microscope and describe their functions
•To describe how to properly use a compound microscope.
•To compare a compound light microscope to a dissecting microscope and electron microscopes.

Label the parts on your microscope picture.
Eyepiece magnification
= 10X

Label the parts on your microscope picture.
Eyepiece magnification = 10X
ArmSupportproper place to pick up/carry

Label the parts on your microscope picture.
Eyepiece/ocular lens magnification = 10X
ArmSupport
Stage – slide is placed here for viewing

Label the parts on your microscope picture.
Eyepiece/ocular lens magnification = 10X
ArmSupport
Stage support
Coarse adjust – general focus. Use
ONLY on Scanning power.

Label the parts on your microscope picture.
Eyepiece/ocular lens magnification = 10X
ArmSupport
Stage support
Coarse adjust – general focus
Fine Focus – use for high power focus

Label the parts on your microscope picture.
Eyepiece/ocular lens magnification = 10X
ArmSupport
Stage support
Coarse adjust – general focus
Fine Focus – high power focus
Base – supportplace other hand here when carrying.

Label the parts on your microscope picture.
Light source
Has a dimmer switch to adjust the amount of light
Eyepiece/ocular lens magnification = 10X
ArmSupport
Stage support
Coarse adjust – general focus
Fine Focus – high power focus
Base support

Label the parts on your microscope picture.
Diaphragm – also adjusts amount of light-works like the pupil of your eye.
Light source
Eyepiece/ocular lens magnification = 10X
ArmSupport
Stage support
Coarse adjust – general focus
Fine Focus – high power focus
Base support

Label the parts on your microscope picture.
Stage clips – holds slide
Diaphragm – adjusts amount of light
Light source
Eyepiece/ocular lens magnification = 10X
ArmSupport
Stage support
Coarse adjust – general focus
Fine Focus – high power focus
Base support

Label the parts on your microscope picture.
Objective Lenses
Scanning = 4X (shortest)
Low Power = 10X (med. length
Stage clips – holds slide
Diaphragm – adjusts amount of light
Light source
Eyepiece/ocular lens magnification = 10X
ArmSupport
Stage support
Coarse adjust – general focus
Fine Focus – high power focus
Base support

Label the parts on your microscope picture.
Revolving nosepiece
Objective Lenses
Scanning = 4X (shortest)
Low Power = 10X (med)
High power = 40X (longest)
Stage clips – holds slide
Diaphragm – adjusts amount of light
Light source
Eyepiece/ocular lens magnification = 10X
ArmSupport
Stage support
Coarse adjust – general focus
Fine Focus – high power focus
Base support

Label the parts on your microscope picture.
Tube
Revolving nosepiece
Objective Lenses
Scanning = 4X (shortest)
Low Power = 10X (med)
High power = 40X (longest)
Stage clips – holds slide
Diaphragm – adjusts amount of light
Light source
Eyepiece/ocular lens magnification = 10X
ArmSupport
Stage support
Coarse adjust – general focus
Fine Focus – high power focus
Base support

Magnification of a Compound Microscope
•Because you are looking through multiple lenses the lenses have a
“compounding” effect.
•The eyepiece always magnifies 10X
Eyepiece Objective Lens Magnification
Scanning lens (red)
Low Power (yellow)
High Power (blue)
10X
10X
10X

Magnification of a Compound Microscope
•Each of the objective lenses have their own magnification
Eyepiece Objective Lens Magnification
Scanning lens (red)
Low Power (yellow)
High Power (blue)
10X
4X
10X 10X
10X
40X

Magnification of a Compound Microscope
•You then multiply the eyepiece with the objective lens to determine the total magnification
Eyepiece Objective Lens Magnification
Scanning lens (red)
Low Power (yellow)
High Power (blue)
10X
4X 40X
10X 10X 100X
10X 40X
400X

•Always carry the microscope with one hand on the Arm and one hand on the
Base.
•Wear your glasses, the microscope will focus to your eyesight!
• Keep both eyes open, your brain will learn to ignore the other eye.
• Never use the coarse adjustment after you turn past scanning (4X) power.

 Always start on scanning power.
 Bring the stage all the way up and focus the specimen using the course adjust.
 Re-center so what you want to focus is at the pointer!
 Turn to high power only after re-centering
 Use the FINE FOCUS ONLY for low and highest power. NEVER use the course adjustment after you turn past scanning power.

1. Don’t even think of starting your drawing unless you have a pencil
(colored are even better) drawings in pen are unacceptable. This is for two reasons:
(a) You can erase pencil!
(b) You can shade in areas more easily in pencil
2.
Take time to add detail to your drawing. Use your pencil to shade and give your drawing some detail and depth. Use colored pencil when appropriate to add color.
3. In the upper left hand corner of each circle include the specimen name as written on the slide label. In the upper right hand corner, include the magnification (100x or 430x).
4.
Label Everything you identify!
Cheek cell 100X
Cell membrane nucleus

1. Gather a very thin slice/piece of whatever your specimen is. All samples should be paper thickness or thinner!
2. Place ONE drop of water directly over the specimen.
The drop should be about ½ the size of a dime.
3. Place the cover slip at a 45 degree angle with one edge touching the water drop, and let the cover slip drop onto the drop of water.
4. Do not push down on the cover slip.
5. Try to avoid bubbles!

Microscope Parts
Label with structure and function
Microscopes
Magnification of Lenses
Eyep iece
Objectiv e Lens
Magnificat ion
Scanning lens
(red)
Low Power
(yellow)
High Power
(blue)
10X
10X
10X