Investigating Aerobic and Anaerobic Respiration Background information: • Yeast is a microscopic fungus that respires aerobically and anaerobically depending on the environment- very similar to humans • Yeast – as well as humans- use glucose to produce chemical energy- ATP Questions to answer: • What substances are needed for cellular respiration to begin? (Look back at your notes if necessary to find the two molecules needed for your cells to produce energy) • When cells or yeast cells go through cellular respiration- what three molecules are produced? Problem? • How can we design a lab to observe anaerobic respiration? Hypothesis: • If……….. Then…………. Lab Design • You have the following materials to use to design an investigation to observe anaerobic respiration. How will you set up your lab? • Materials: – 50 ml of apple cider – Balloon – 2 grams of yeast – Erlenmeyer flask Safety • Long pants • Safety glasses • Closed toe shoes Variables • Independent• Dependent• Control- Procedures • Gather necessary materials • Measure 50 ml of apple cider and put into your Erlenmeyer flask. • Add 2 grams of yeast to flask/cider • Cover IMMEDIATELY with a balloon • Let set. Record observations after 30 minutes and after 3 hours Observations: • What did you observe about your set up: – After 30 mins? – After 3 hours? Circumference after 30 minutes Circumference after 3 hours(min) Conclusion: • What did you observe about the balloon/flask set up on day one? • Did your balloon inflate? If so, what substance filled the balloon? • Briefly explain- using correct vocabulary termshow the substance was produced. • Did the yeast carry out aerobic or anaerobic respiration? How do you know? • Did the control balloon inflate? Why or why not?