Apical Meristems Read as a group: Recall that meristemic cells (the unspecialized cells that divide enabling the plant to grow) are located a) near the tips or the roots and shoots, and b) in the growing parts of the shoot, just below the outside layer of the stem of woody plants. Apical meristems: are the undifferentiated cells at the tips of the plants root and shoot; cells divide to make the plant grow longer Roots: There are 4 distinct zones of the growing tip of a root. 1. 2. 3. 4. The root cap (which is not growing) The meristem region of cell division The region of elongation The region of maturation When meristems first divide in the tips if a root, many of the cells begin to elongate This makes the root longer and pushes its way through the soil (they can actually become 10X longer) As they elongate they begin to specialize as dermal, ground, or vascular tissue depending on their location. Once they are matured they cannot grow or divide anymore Shoots: The apical meristems in shoots are located as buds at various regions of a stem. Location: 1. At the very tip of the stem allowing the plant to grow longer upwards 2. At the different points along the stem allowing the plant to grow side branches These buds contain meristem cells that will mature into stem, leaf, and flower tissue. Activity: 1. Colour the root tip into its 4 regions. Take note of the region and recognize what the cells look like in this region? 2. Label the shoot system with the following: apical meristem bud, lateral bud, leaf, and new leaf. Apical Meristems – enables the plant to grow longer Vascular tissue maturation The only cell division in a root takes place in the apical meristem. Then the cells elongate, then differentiate into the various types of tissue (dermal, ground, or vascular) and finally they mature where they will not longer grow or divide. Apical meristems occur at the tips of the stem to allow the plant to grow longer upwards, and lateral buds can also occur on the stem allowing the plant to grow side branches. The bud will mature into stem, leaf, or flower tissue. Lateral Meristems Read as a group: Recall that meristemic cells (the unspecialized cells that divide enabling the plant to grow) are located a) near the tips or the roots and shoots, and b) in the growing parts of the shoot, just below the outside layer of the stem of woody plants. Lateral meristems: are the undifferentiated cells under the bark in the stems and roots of woody plants; that divide enabling the plant to grow wider Lateral meristems form 2 cylinders, one inside of the other, that run the full length of the shoots and roots. As the plant grows in diameter, the outer lateral meristem produces new dermal tissue called cork to replace the old epidermal tissue The inner lateral meristem produces new phloem tissue on its outer surface and new xylem tissue towards its interior. Activity: 1. Look at the following diagram of a cross section of a tree. Fill in the blanks of the statements below with the following words: - Xylem - Increase - Age - Phloem - Lifespan - Cork - Non-woody - Diameter - Root 2. Take a look at the pictures of the tree trucks. Estimate the age of the trees by counting the inner xylem rings. Lateral Meristems – enables a woody plant to grow wider Cork Outer lateral meristem (cork cambium) Phloem Inner lateral meristem (vascular meristem) Xylem rings The ____________ and the __________ form the bark of the tree, whereas the ____________ form rings of the inner part of the tree The xylem keeps accumulating and causes the tree trunk to _____________in ____________ year after year We can read the _______ of a tree because each year it grows there is a noticeable ring of new xylem Many plants (______________ plants) do not contain lateral meristems thus they cannot grow thicker than their original _______ size and so they remain quite small and have a shorter _______________ (approx. 1 year) Estimate the age of the tree: Age ______ Age ______ Vegetative Reproduction and Mass Producing Plants Read as a group: Vegetative reproduction is the process in which a plant produces genetically identical offspring from its roots or its shoots. In other words, it is when a plant produces a clone of itself. Example: Strawberry plants reproduce by sending out shoots called runners across the surface of the soil. When the meristems in the tips contact the ground they begin to grow into shoots and a new plant develops. Example: A cluster of poplar trees are actually clones from a single parent. The cluster is actually not a bunch of trees but one tree with a bunch of stems. We can tell this because in the fall they change color at the exact same time! This process has been very useful to farmers, gardeners, and agricultural scientists because can literally take a plant, cut it up and clone that plant many times over. Tissue Culture Propagation: a method of growing many identical offspring by obtaining individual plant cells from one parent, growing these cells into calluses, and then whole plants - - Cells from certain plant roots are grown in containers with special chemicals that reverts them back into undifferentiated cells which begin to divide (like meristem cells) As they divide they form a clump called a callus These calluses can be separated over and over again to produce a number of plants These plants are genetically identical to the original parent plant Activity: 1. Match the pictures with the correct definition. 2. As a group come up with 3 reasons why it is helpful to humans to use tissue culture propagation. Vegetative Reproduction and Mass Producing Plants ____ Vegetative reproduction ____ Mass production of plants ____ Leaf cutting produced all these shoots ____ Runners from a plant ____ Clones ____ Callus ____ New plant from a callus ____ Potatoes cut form spouts that can become a new potato plant 3 Reasons Why we Take Advantage of Tissue Culture Vegetative Reproduction 1. 2. 3. Vegetative reproduction is the process in which a plant produces genetically identical offspring from its roots or its shoots. In other words, it is when a plant produces a clone of itself. Tissue Culture Propagation: a method of growing many identical offspring by obtaining individual plant cells from one parent, growing these cells into calluses, and then whole plants