S.K.H Li Ping Secondary School Group no.: C20 ---------------------------------• Man Ka Yan, Grace • Leung Yu Kiu, Pearl • Yuen Yuk Man, Alex • Yeung Siu Hung, Eric • Kwok Tsz Kin, Ryan Problem Reference books Some anti-microbial species Cinnamon Garlic Ginkgo Oleander Agar test Objective: • To find out which herbs is the best to inhibit the growth of mould. Setups: Samples No. of day that the mould grows on agar jelly Control 17 Oleander 17 24 Ginkgo 37 Garlic 83 Cinnamon 0 10 20 30 40 50 Days 60 70 80 90 神龍本草經 Shen Long Ben Cao Jing 摘要:菌桂. 味辛溫.主百 病.養精神和 顏色.久服輕 身不老.面生 光華 民間驗方 千金翼薰衣濕香方 •桂皮155克 •薰陸香250克 •詹糖香155克 •藿香各90克 •甲香60克 主治:薰衣 ;同時又具解毒止痛消癮疹 的作用,可增加皮膚的抗病能力。 Effective components The effective components of cinnamon are cinnamic aldehyde & cinnamic acid Project Name Cinnamon Substances Investigation 剋 霉 桂 Extraction Method •Boiling Extraction Method •Steam Distillation Extraction Method Solvent: •95% ethanol •Rice wine Why use ethanol and Rice wine? Ethanol and Rice wine: • DIY approach • High dissolving power for cinnamic aldehyde and acid Results Method Colour Odour Steam Boiling distillation Light Brown Milky Solvent Extraction (Rice wine) Solvent Extraction (95% Ethanol) Light Brown Brown Cinnamon smell Deduction • The effective components in cinnamon have been extracted • Chemical Structure VS Chemical Tests Cinnamic aldehyde Cinnamic acid Results Tollen reagent KMnO4 K2Cr2O7 Bromine Fehling 2,4-Dinitrophenylhydrazine Pure Cinnamic Aldehyde + + + + + + Pure Cinnamic Acid + - + - - - In 95% Ethanol + + + + + + Rice Wine + + + + + - + + + + + + + + - + + - By Steam Distillation By Boiling Results Tollen reagent KMnO4 K2Cr2O7 Bromine Fehling 2,4-Dinitrophenylhydrazine Pure Cinnamic Aldehyde + + + + + + Pure Cinnamic Acid + - + - - - In 95% Ethanol + + + + + + In 29% Rice Wine + + + + + - + + + + + + + + - + + - By Steam Distillation By Boiling Infrared spectrometry (IR) Ultra-violet spectrometry (UV) High Pressure Liquid Chromatography (HPLC) Objective • Qualitative: Present or not? • Quantitative: How many? Chromatogram of Standard 4ppm standard (cinnamic aldehyde and cinnamic acid) Cinnamic acid Cinnamic aldehyde Retention Time(minutes) 12.668 14.55 Integrated Area(mAUS*a) 278.8512 411.25055 Samples Five samples were prepared in different way By Boiling Rotary Ground Cortex +Water & Boiling 1 2 3 Sample 1 Rotary+ Boiling Conc. of Cinnamic acid, (X10-6M) Conc. of Cinnamic aldehyde, (X10-6M) Conc. of Cinnamic substances, (X10-6M) 132.2 + 117.3 117.3 = 249.5 249.5 Conclusion I Ground+ Boiling 2 Variations were due to their Cortex + Boiling 3differences in: 4 •Brand Cortex + Extraction (Rice wine) •Origin Cortex + Extraction •Treatment 5 (95% Ethanol) 102.7 225.4 328.1 67.3 51.2 118.5 37.5 3442 3479.5 42.5 9720 9762.5 By Solvent Extraction + 4 Rice wine Cortex 95% Ethanol + 5 Conc. Of Cinnamic substances, (X10-6M) 10000 9762.5 Conclusion III II Conclusion IV -6 250 x 10 M are Effective components Rice wine is good enough! highly dissolved alcohol! is enough to killinmould 3479.5 ■ Boiling ■ Solvent Extraction 250 249.5 328.1 118.5 0 1 2 3 4 5 (Sample) Cinnamon Substances Investigation (CSI) 1. In cinnamon, aldehyde content >> acid content 2. Aldehyde oxidized to acid 3. Both have strong anti-mould power 4. Rice wine is a cheap and effective solvent Investigation - Problem A • Mould grows on silicon sealant How to make Cinnamon cortex Honey wax Rice wine Solution •Apply anti-mould coating Investigation - Problem B • Athlete's foot / Disgusting smell A homeopathic remedy Heinerman’s Encyclopedia of Fruits, Vegetables and Herbs, 1998 For athlete’s foot…soaking one’s feet in a lukewarm brew of cinnamon and water to inhibit fungi growth… “Arresting the fungus among us” http://www.ucsf.edu/today How to make • Painting concentrated cinnamic extract on cotton shoe insole Is it safe? LD50 ( pure cinnamic acid) for Rats = 2.2g/kg 157kg cinnamon is required to kill a 45kg man Be careful Allergic Pregnant Asthma Summary • Just a tip of an iceberg • Great potential for future development End of Presentation Some references • Christian, Gary D.; Analytical Chemistry, 5th edition, 1994, John Wiley & Sons, pg. 112-114, 245-248. • 柯銘清 編, 中草葯有效成份---理化與葯理特性, 湖南科學技術 出版社, pg. 21-22. • 宋小平, 韓長日 編, 香料與食品添加劑制造技術, 科學技術文獻 出版社, pg. 80-84. • Morrison and Boyd, Organic Chemistry, 3th edition, 1977, Practice-Hall, pg. 186, 634-635, 639-640. • Graham, T.W., Organic Chemistry, 2th edition, 1980, John Wiley & Sons, p.259, 266, 712, 740. • Skoog, D.A., Holler,F.J., Principles of Instrumental Analysis, 5th edition, 1998, Harcourt Brace & Company Chapter 14, pg.330-331