Amgen Lab 2: Restriction Analysis of pARA and pKAN-R—CLASS SET
Directions: Answer the following study questions on YOUR OWN PAPER and in COMPLETE SENTENCES!
1. Define gene expression.
2. What is a selectable marker?
3. Describe the growth of bacteria that carries an antibiotic resistant plasmid vs. those that do not carry
antibiotic resistant plasmids.
4. Which plasmid carries the ampicillin resistance gene? Which plasmid carries the kanamycin
resistance gene?
5. Draw the pARA plasmid.(see overhead for new plasmid)
5. What is the purpose of lab 2?
6. Draw the pKAN-R plasmid.(see overhead for new plasmid)
7. What is beta lactamase?
8. What is the purpose of the AraC protein? Which plasmid contains the gene for this protein?
9. What is the purpose of the pBAD?
10. What do the sites labeled “BamI and HindIII” represent? Define this site. (look at end of reading)
11. What is phosphotransferase? What plasmid has the gene for making this protein?
12. What is “rfp”?
13. Where did the original fluorescent protein gene come from?
14. Define “wild type”.
15. What is directed evolution?
16. Which plasmid contains the “rfp” gene?
17. What was in the A+, A-, K+, K- tubes? Did we get the expected results? (look at gel results and
discuss them in-depth—include lane numbers)
18. What was the next step? What did we do in lab 3: Ligation?