Federal Centre of Breeding Research on Cultivated Plants
Selection, structure and multiplication of the
working collection
Christoph U.Germeier
Avena genetic resources for quality in human consumption (AVEQ)
Startup Meeting, Clermont Ferrand 2007
Structure of the Working Collection as proposed
In total 600 accessions:
- 100 wild species accessions
- 200 landraces
- 200 obsolete cultivars
- 100 modern cultivars (to be provided by breeders)
70 accessions selected for quality traits from the previous project:
(seed weight, kernel weight, protein content)
Avena genetic resources for quality in human consumption (AVEQ)
Startup Meeting, Clermont Ferrand 2007
Maximizing variability in the working collection
a) European countries sensu latu (including non EU countries like Albania,
Belarus, Moldova, Russia and the Ukraine) should be represented equally.
b) Available time information (acquisition, collecting, registration) was
classified into <1900, 1900-1950, > 1950 (and actual cultivars). Time
classes should be represented as equal as possible
c) Botanical varieties have been determined by genebanks for the cultivated
species (A.strigosa, A.abyssinica, A.byzantina, A.sativa) in variable extent.
This information was used for probable duplicates also in other collections.
It was sought to get botanical varieties represented as equally as possible
d) If collecting site information was available it was sought first to represent
all levels of elevation with a special emphasis to higher elevations (cold
tolerance) and latitudes. Further geographic information was used to get as
much as possible geographic distance.
This approach favours accessions with more complete passport data.
Avena genetic resources for quality in human consumption (AVEQ)
Startup Meeting, Clermont Ferrand 2007
Wild species accessions - diploids
Species
A.atlantica
A.canariensis
A.damascena
A.hirtula
A.longiglumis
A.prostrata
A.wiestii
A.clauda
(incl. A.eriantha)
A.pilosa
A.ventricosa
(incl. A. bruhnsiana)
EADB EURISCO Working Collection
Multiplication 2007
Accs.
Accs.
Accs. Holders
Accs. Holders
12
2
7 GBR016:5, RUS001:2
43
14
11 RUS001:5, GBR016:4, DEU146:2
1 DEU146:1
16
13
10 GBR016:3, POL003:3, RUS001:3,
3 POL003:3
DEU146:1
39
18
10 RUS001:4, GBR016:3, ESP004:2,
1 POL003:1
POL003:1
37
19
10 ESP004:4, GBR016:3, RUS001:2,
DEU146:1
13
2
7 GBR016:4, RUS001:2,DEU146:1
32
23
5
DEU146:2, RUS001:2, GBR016:1
2 DEU146:2
34
17
11 RUS001:7, BGR001:2, DEU146:2
1 BGR001:1
26
14
8
12
6
7
RUS001:4, GBR016:2,
BGR001:1, GBR011:1
RUS001:5, DEU146:1, GBR016:1
Avena genetic resources for quality in human consumption (AVEQ)
Startup Meeting, Clermont Ferrand 2007
Wild species accessions - tetraploids
Species
A.macrostachya
A.barbata
(incl. A. hirsuta)
A.vaviloviana
A.agadiriana
A.insularis
A.magna
A.murphyi
EADB EURISCO
Accs.
Accs.
3
1
353
148
64
6
63
62
2
1
33
18
5
Working Collection
Multiplication 2007
Accs. Holders
Accs. Holders
2
GBR016:1, RUS001:1
17 DEU146:10, RUS001:3,
8
DEU146:8
ESP004:2, AUT001:1, GBR016:1
10 RUS001:7, GBR011:2, CZE047:1
7
GBR016:4, RUS001:3
1
RUS001:1
12 RUS001:5, GBR016:4, DEU146:1,
1
DEU146:1
GBR011:1, HUN003:1,
10 GBR016:5, RUS001:3,
DEU146:1, ESP004:1
Avena genetic resources for quality in human consumption (AVEQ)
Startup Meeting, Clermont Ferrand 2007
Wild species accessions - hexaploids
EADB EURISCO
Accs.
Accs.
1349
467
Species
A.fatua1
A.hybrida
A.sterilis1
(incl. ludoviciana, persica,
trichophylla)
A.occidentalis
16
2
2355
1686
7
7
Working Collection
Multiplication 2007
Accs. Holders
Accs. Holders
18 DEU146:11, AUT001:2,
12 DEU146:11,
BGR001:2, CZE082:1,
BGR001:1
ESP004:1, GBR016:1
3
BGR001:1, DEU146:1,
1 DEU146:1
GBR016:1
17 DEU146:8, RUS001:4,
7 DEU146:7
AUT001:2, ESP004:2,
GRC005:1
5
RUS001:5
Avena genetic resources for quality in human consumption (AVEQ)
Startup Meeting, Clermont Ferrand 2007
Landraces and obsolete cultivars marginally cultivated species
Species
A.abyssinica
A.strigosa
including
A.brevis
A.nuda
EADB EURISCO
Accs.
Accs.
160
355
54
(407)
Working Collection
Accs. Holders
12 RUS001: 8, DEU146: 2,
GBR011: 1, BGR001: 1
47 RUS001:17, DEU146:9,
PRT004:5, ESP004:4,
GBR011:3, POL003:3,
BGR001:2, SWE002:2,
AUT001:1
Multiplication 2007
Accs. Holders
4
DEU146:2, BGR001:1,
RUS001:1
24 DEU146:8, RUS001:8,
POL003:3, SWE002:2,
AUT001:1; BGR001:1,
FRA040:1
Avena genetic resources for quality in human consumption (AVEQ)
Startup Meeting, Clermont Ferrand 2007
Landraces, obsolete and modern cultivars
Working
1880- 1890- 1910- 1930- 1950- 1970n.d. 1809 1832
>1990
collection
1889 1909 29
49
69
89
Wild species 188 28
2
7
85
66
A.abyssinica 12 2
5
1
1
3
A.strigosa
47 8
10
7
4
12
6
A.byzantina 49 2
13
5
6
14
9
A.sativa
466 119
1
1
6
7
68
53
43
83
85
Multiplied
2007
wild
A.abyssinica
A.strigosa
A.byzantina
A.sativa
1880- 1890- 1910- 1930- 1950- 1970n.d. 1809 1832
>1990
1889 1909 29
49
69
89
37 12
2
2
16
5
4 1
1
2
24 5
3
3
2
7
4
31 2
2
5
5
8
9
318 90
1
1
6
6
30
37
36
60
51
Avena genetic resources for quality in human consumption (AVEQ)
Startup Meeting, Clermont Ferrand 2007
Summary on genebank level
Project Partners
WC M07
POL003
FRA040
BGR001
CZE047
ROM007
ITA037
EST001
32
45
27
37
14
13
13
32
42
13
29
10
11
13
Total
181 150
Holder
RUS001
DEU146
Breeders
SWE002
SVK001
AUT001
LTU001
UKR007
IRL029
NLD037
LVA010
AUT005
USA029
Total
Other Genebanks
WC M07 Holders
to access again WC
199
59
129
50
25
15
11
7
5
5
3
2
2
2
115
26
24
15
6
5
5
3
2
2
2
455
264
GBR016
ESP004
GBR011
PRT004
HUN003
GRC005
Total
Avena genetic resources for quality in human consumption (AVEQ)
Startup Meeting, Clermont Ferrand 2007
43
31
26
10
6
6
122
Total Summary
status level
WC M07
diploid wild species
86 8
tetraploid wild species 59 9
hexaploid wild species 43 20
marginally cultivated species
A.abyssinica
12 4
A.strigosa
47 24
genebank level
WC M07
Project Partners
181 150
Other Genebanks 455 264
Access again
122
Total
758 414
cultivated hexaploid oat
A.byzantina
49 31
A.sativa ssp. nudisativa 37 23
A.sativa ssp. sativa
416 318
Avena genetic resources for quality in human consumption (AVEQ)
Startup Meeting, Clermont Ferrand 2007
Standards
Standards as outlined in the proposal:
WP2: A panel of market varieties should be included as reference standards.
WP3: Five to ten standards in five replicdations.
WP4: Field experiments with a minimum of five ? ten standards in five
replications.
Standards - Questions
Only modern cultivars or also other material?
Use material multiplied at the multiplication sites or material provided from
breeders?
How many standards to use in field experiments – in laboratory analysis?
(e.g. use a reduced set selected by performance in field for lab
analysis
Avena genetic resources for quality in human consumption (AVEQ)
Startup Meeting, Clermont Ferrand 2007
Standards suggested so far:
Nordic region (Estonia, Sweden):
Belinda (SW): represents the mid-late varieties in this region;
currently the main variety in Sweden, Norway, Finland,
also listed in the Baltic countries.
Jaak (Jogeva): One of the Estonian latest-bred varieties, 2-3 days
earlier than the other Estonian
a very early variety would be the Norwegian Leila
Western Europe (France):
Auteuil: most cultivated spring cultivar in France,
was already standard in the previous project
Evora: second most cultivated winter cultivar in France,
Avena genetic resources for quality in human consumption (AVEQ)
Startup Meeting, Clermont Ferrand 2007
Standards suggested so far:
Central Europe (Czech Republic, Germany, Poland):
Ivory: has been reported to accumulate less DON because of low
hull content.
Krezus:
Saul: naked variety with lower fat
South Europe (Italy): Argentina most cultivated in past
Genziana very new variety
South East Europe (Bulgaria, Romania):
Mures
Mina: naked cultivar
Avena genetic resources for quality in human consumption (AVEQ)
Startup Meeting, Clermont Ferrand 2007
Standards used in Multiplication 2007
Origin
Accession
Name
DEU FRA POL
Total
plots
BGR
BGR001
A7BM0001
Mina
13
BGR
BGR001
A7BM0011
Obraztcov Chiflik 4
8
1
1
10
CZE
CPVO
20022073
Atego
8
1
3
12
CZE
CZE047
03C0702014
Atego
5
5
DEU
CPVO
20030329
Flämingskurz
5
DEU
DEU101
HA 1176
Flämingsprofi
8
3
1
12
DEU
CPVO
20040091
Ivory
5
1
3
9
EST
EST001
00004
Jaak
11
2
2
15
FIN
BOREAL VELI
Veli
11
2
7
20
13
5
Avena genetic resources for quality in human consumption (AVEQ)
Startup Meeting, Clermont Ferrand 2007
Standards used in Multiplication 2007
Origin
Accession
Name
DEU FRA POL
Total
plots
2
1
3
FRA
FRA040
30040
Auteuil
FRA
FRA040
20127
Evora
1
1
FRA
FRA040
30037
Ourasi
1
1
ITA
ITA037
8
Argentina
8
1
5
14
ITA
ITA037
700
Genziana
7
1
6
14
POL
POL003
Krezus
Krezus
8
2
20
30
POL
POL003
Stoper
Stoper
8
2
13
23
ROM
ROM007
ROM007-16701
Mures
5
2
3
10
SWE
CPVO
19960125
Belinda
13
2
10
25
Avena genetic resources for quality in human consumption (AVEQ)
Startup Meeting, Clermont Ferrand 2007
Observations in multiplication plots in Quedlinburg
Emergence in all (6, 5, 3) rows 1 m row
Growth habit in youth stage
BYDV, visual score (%plot and score on one (diseased)
plant (1-9 dead ) and ELISA on single plants
Heading date
Mildew (1-9)
Panicles: orientation of lateral branches,
spikelet attitude, classification
Lodging
Grain shedding
Awnedness
Awn type
Grains per Spikelet
Crop height (plant length)
Panicle length
Avena genetic resources for quality in human consumption (AVEQ)
Startup Meeting, Clermont Ferrand 2007
Thanks for your attention
Avena genetic resources for quality in human consumption (AVEQ)
Startup Meeting, Clermont Ferrand 2007
Landraces and obsolete cultivars Avena byzantina
EADB:
ESP (261), GRC (115), ITA (161), PRT (94), DEU (23), RUS (24)
ALB (23), CZE (21), SCG (21), CYP (19), FRA (12),
GBR (10), ROU (7), BGR (4), SWE (4)
EURISCO:
ESP (366), ITA (166), GRC (89), PRT (60), RUS (30), ALB (28),
DEU (24), CYP (20), FRA (10), SVK (5), GBR (5), AUT (4),
BGR (4), CZE (4), HRV (4), POL (4), SWE (4)
Working
collection
49
ITA (7), GRC (5), ALB (3), DEU (3), TUR (2), FRA (2), PRT (2),
HRV (2), AUT (2), CYP (2), CZE (2), ROM (2), YUG (2),
SVK (1), GBR (1), UKR (1), RUS (1), MAR (1), LBY (1)
Multiplied
2007
31
ITA (5), DEU (3), ALB (3), HRV (2), GRC (2), FRA (2),
ROM (2), AUT (2), YUG (2), CZE (2), TUR (1), SVK (1),
GBR (1), ESP (1), MAR (1), LBY (1)
Avena genetic resources for quality in human consumption (AVEQ)
Startup Meeting, Clermont Ferrand 2007
Landraces and obsolete cultivars - naked oat
In working collection: 37
A.byzantina ssp. nudisativa
DEU (1)
A.sativa ssp.nudisativa
CZE (6), GBR (4), FIN (2), POL (2),
DEU (2), FRA (2),
Multiplied 2007: 23
ROM, BEL, AUT, GRC, HUN, ESP,
BGR, NOR, CSK, ITA, LTU, BYS,
PRT, DNK, YUG, SWE, RUS, NLD (1)
A. byzantina ssp. nudisativa DEU (1)
A. sativa ssp.nudisativa
CZE (4), FRA (2), POL (2), DEU (2),
AUT, BGR, CSK, DNK, GBR, SWE,
GRC, ITA, LTU, PRT, ROM, FIN (1)
Avena genetic resources for quality in human consumption (AVEQ)
Startup Meeting, Clermont Ferrand 2007