Warm-up 3/3/16: On the same sheet of notebook
paper that you wrote on yesterday, write today’s
questions and answers. Place Vocab in folders. DO
NOT TOUCH LAB MATERIALS!!!!
1. The cell membrane is one of the most important
organelles within a cell. Why? (In other words,
what is the job of the cell membrane?)
2. The cell membrane is made up of two main
macromolecules. What are they?
3. The cell membrane can be compared to a cell
phone password. How is the cell membrane and a
cell phone password similar?
Announcements
• If you turn in assignments after the due date,
it will be an automatic 50. NO EXCEPTIONS.
• New Bathroom Policy
• If your absent, please see the syllabus for the
absent work policy. Unexcused absences will
have 2 days to complete work. Excused
absences will have 5 days to complete work.
Bubble Lab: A Model Cell Membrane
• In this investigation lab you will explore a
model that displays some of the features of a
cell membrane.
First, Label a blank sheet of notebook paper
with the following
Name, Date, Block, “Bubble Lab: A Model Cell
Membrane”
Next, Draw the data table below onto
your notebook paper.
Procedure
Step #
#1
#2
#3
Observations
Observations
Predictions
(actual)
Step 3. Practice Forming a Bubble Film
using the model cell membrane.
Please Share the materials!!!!
• You are going to form a film of bubble solution on
the straw device by submerging the rectangle
into the solution. Then slowly draw it up at a 45
degree angle. Gently move the rectangle up and
down and observe the bubble film.
Bend the cell membrane back and forth.
What property of the cell membrane do you
think this demonstrates?
Cell Membrane
•flexible outer boundary of a cell (like skin)
•Job - to maintain homeostasis (internal balance)
•Referred to as a Fluid Mosaic Model – moves like a fluid with
embedded proteins
Protein
Phospholipids
Bi-layer
Cell Membrane
Procedure Step 1
What do you think will happen if we insert a
dry straw into the model membrane? Record
your answer in the predictions column for
procedure step 1
1.Reform a bubble film if necessary. Insert a
dry straw into the bubble membrane and
then dry the straw. Record your
observations.
Procedure Step 2
• What do you think will happen when we coat
the straw in the bubble solution and then
insert it into the model cell membrane?
Record your predictions for procedure Step 2
2. Reform a bubble film if necessary. Now coat
the straw completely with the solution. Insert
the coated straw into the bubble membrane.
Record your observations.
Procedure Step 3
• What do you think will happen when we place a
coated rubber band into the model membrane?
Record your predictions for procedure step 3.
• Reform a bubble film if necessary. Then form an
opening in the bubble membrane by floating the
rubber band on the film and then popping the
inside with your pencil. Try to pass the straw
through the open circle in the model membrane.
Record your observations.
Cell Membrane
1.
Membrane Protein
a.Transport Proteins - help larger molecules
get across the cell membrane
b. Enzymes - help speed up the reaction.
c. Chemical Markers – help cells to identify
each other
d. Cell Signaling – messages from outside of
cell are communicated to the inside
Procedure Step 4
• What do you think will happen we you remove
the paper clip from the model membrane.
Record your predictions for Procedure Step 4.
• Use the straight end of the paper clip to gently
remove the circle of thread from the bubble
membrane. Record your observations.
Analysis
1. What does it mean to say that the cell membrane is fluid?
2a. In procedure step 1, we inserted a dry straw into the bubble membrane. What happened to
the cell membrane?
3a. In procedure step 2, we coated the straw in the bubble solution and then passed it through
the model cell membrane. What happened to the cell membrane?
3b. Why was the straw able to pass through the cell membrane when it was coated in the bubble
solution?
4. In procedure step 4, we formed an opening through the bubble membrane by floating a
rubber band on the film and then popping the inside a pencil. Then we removed the rubber
band. What happened to the bubble membrane when we removed the rubber band?
5. What characteristic of the cell membrane do you think you are observing in procedure step 4?
(In other words, what does this tell us about our real cells in our body?)
Cellular Transport
What is Homeostasis?
• Maintaining a constant internal BALANCE.
– Plants and animals regulate things like: pH, water,
temperature, glucose, salts, etc.
• CELL MEMBRANE controls what goes in and
out = maintains homeostasis!
What is the PHOSPHOLIPID BILAYER?
• The CELL MEMBRANE!
• Phospholipid bilayer: two layers ofLIPIDS that
make a flexible barrier
• ALSO:
– PROTEINS help let things in an out
– CARBOHYDRATES help identify particles
• Selectively-permeable: only lets some things
move through
What does Semi-Permeable Mean?
• Let’s some things in, but not others.
• SEMI – part; selective
• PERMEABLE – things can get in and out
How does the cell membrane help
maintain homeostasis?
1. The PORES let very SMALL molecules to
move in and out freely (O2, CO2& H2O)
1. The PROTEINS allowBIGmolecules to get in
and out of the cell (Salt & Sugar)
The PhospholipidBilayer
Phosphate Heads:
HYDROPHILLIC =
water-loving (stay on the
outside of the membrane)
Fatty Acid Tails:
HYDROPHOBIC=
water-fearing (stay on the
inside of the membrane)
Other Molecules In the Bilayer
Check Point 1
1. What is homeostasis?
2. How do your cells help maintain
homeostasis?
3. What molecules make up the Cell
Membrane?
4. What are 2 ways molecules move through
the cell membrane?
Complete the Cell Membrane Practice
Questions on the back of your
Checkpoints Sheet.
Warm-up 3/4/16: Place your
homework in the folder at your
station. DO NOT TOUCH ANY OF THE
PROJECTS.
• On a sheet of notebook paper, answer the
questions at your table to review “A Model Cell
Membrane” Lab that we completed on
yesterday.
• The questions are taped to your table.
• Write your answers in complete sentences.
3-D CELL PROJECT GALLERY WALK
(15 MINUTES)
As you walk around the room you will review
each others 3-D Cell Projects.
Label your paper “3-D CELL PROJECT GALLERY
WALK” Write your name and today’s date
(3/4/16)
Each project has a number. Please number a
clean sheet of notebook paper from 1-20
3-D CELL PROJECT GALLERY WALK
(15 MINUTES)
• Rate the project on a scale from 1-5:
1=Being Poor and 5= Excellent
• Provide one complement about the project. One
thing that stood out to you about the project.
• Provide one suggestion for improvement about
the project. One thing that you think could be
better about the project.
VOTING TIME!!!
• AT THE BOTTOM OF YOUR SCORE SHEET,
WRITE DOWN WHICH PROJECT IS YOUR ALL
TIME FAVORITE!
• THE PROJECT WITH THE MOST VOTES WILL
RECEIVE A SPECIAL PRIZE ON MONDAY!
HOUSEKEEPING/REVIEW
•
•
•
•
Unit 1 data tracking
Unit 2 Item analysis and data tracking
Unit 3 data tracking
Homework Review: With the people at your
station, compare your answers to the
homework questions. If you have a question
about one of the homework questions. Please
raise your hand and I will come to your
station. (5 minutes)
• The Cell Membrane is Semi-Permeable Review
INTRO TO ECOLOGY IN GREEN COACH
BOOKS (30 MINUTES)
• USE THE ANSWER SHEET GUIDE TO COMPLETE
THE INTRODUCTION TO ECOLOGY IN THE
GREEN COACH BOOKS
Warmup
3/8/16
1.
Copy the remainder
of the cell transport
notes from the
powerpoint provided
at your station.
2.
Complete the Cell
Membrane: A
Phospholipid Bilayer
Review Coloring
worksheet from
yesterday.
Warm-up 3/8/16
1. Turn in Cell Membrane coloring sheet
from yesterday if you did not do so already.

Get out notes on Cellular Transport
LET’S REVIEW THE CELL MEMBRANE!
1. We will quickly go over last week’s homework,
Cell membrane questions

The cell membrane is selectively permeable.
Some things can enter some can’t.
Copyright © 2010 Ryan P. Murphy
• What does a cell want to come in?
– Hint! You are made of cells. Your cells want
the same things you want.
Copyright © 2010 Ryan P. Murphy
• Answer! The cell wants to let in...
– Oxygen.
– Water.
– Food.
• Molecules
– Protein.
– Others
Copyright © 2010 Ryan P. Murphy
• Answer! The cell wants to let in...
– Oxygen.
– Water.
– Food.
• Molecules
– Protein.
– Others
Copyright © 2010 Ryan P. Murphy
• Answer! The cell wants to let in...
– Oxygen.
– Water.
– Food.
• Molecules
– Protein.
– Others
Copyright © 2010 Ryan P. Murphy
• Answer! The cell wants to let in...
– Oxygen.
– Water.
– Food.
• Molecules
– Protein.
– Others
Copyright © 2010 Ryan P. Murphy
• Answer! The cell wants to let in...
– Oxygen.
– Water.
– Food.
• Molecules
– Protein.
– Others
Copyright © 2010 Ryan P. Murphy
• Answer! The cell wants to let in...
– Oxygen.
– Water.
– Food.
• Molecules
– Protein.
– Minerals
Vitamins
Copyright © 2010 Ryan P. Murphy
• What does a cell want to keep out?
– Hint! You are made of cells.
Copyright © 2010 Ryan P. Murphy
• Answer! The cell doesn’t want to let in…
–
–
–
–
Carbon Dioxide.
Waste.
Virus.
Bacteria.
Copyright © 2010 Ryan P. Murphy
• Answer! The cell doesn’t want to let in…
–
–
–
–
Carbon Dioxide.
Waste.
Virus.
Bacteria.
Copyright © 2010 Ryan P. Murphy
• Answer! The cell doesn’t want to let in…
–
–
–
–
Carbon Dioxide.
Waste.
Virus.
Bacteria.
Copyright © 2010 Ryan P. Murphy
• Answer! The cell doesn’t want to let in…
–
–
–
–
Carbon Dioxide.
Waste.
Viruses.
Bacteria.
Copyright © 2010 Ryan P. Murphy
• Answer! The cell doesn’t want to let in…
–
–
–
–
Carbon Dioxide.
Waste.
Viruses.
Bacteria.
Copyright © 2010 Ryan P. Murphy
• Answer! The cell doesn’t want to let in…
–
–
–
–
Carbon Dioxide.
Waste.
Viruses.
Bacteria.
Harmful
bacteria
Copyright © 2010 Ryan P. Murphy
• The Cell Membrane controls movement
(cellular traffic) in and out the cell.
Copyright © 2010 Ryan P. Murphy
Warmup 3/7/16: Use the green coach books
at your station to complete the Introduction
to Ecology questions. Use the answer sheet
that you received last week.
Once you finish:
1. Complete Cell
Membrane
Review
Coloring
Activity
2. Copy Cell
Transport
Guided Notes
from Friday
Finish Unit 2 Item Analysis
• Be sure to track your test and quiz data on
your Biology Student Data Tracker.
• We also take this time to track your Unit 1
test and quiz data on your Biology Student
Data Tracker.
Cell Membrane Review
• We will review your homework from Friday
on the Cell Membrane
• You will also complete the Cell Membrane:
A Phospholipid Bilayer Review Worksheet
What is Transport?
• The cell membrane controls what moves in
and what moves out of a cell.
• This action of moving in and moving out is
called TRANSPORT.
What are the 2 main types of
transport?
• PASSIVE TRANSPORT
– Does NOT require energy from the cell (NO ATP)
• ACTIVE TRANSPORT
– DOES require energy from the cell (NEEDS ATP)
Video Inquiry
1. Describe what you saw in the video clip.
2. Which took more energy: the truck going up the
hill or down the hill?
http://www.youtube.com/watch?v=f3HQd8M1C4o

Passive transport: The movement of
molecules from a more crowded to a less
crowded area without the use of energy.
Copyright © 2010 Ryan P. Murphy
What are the 3 types of Passive
Transport?
• DIFFUSION
– movement ofPARTICLES
• OSMOSIS
– movement of WATER
• FACILITATED DIFFUSION
– uses aPROTEIN to help movePARTICLES

Diffusion: Random movement of
molecules.
 From
high to low concentrations.
Copyright © 2010 Ryan P. Murphy

Diffusion: Random movement of
molecules.
 From
high to low concentrations.
Copyright © 2010 Ryan P. Murphy
• Why do substances always flow from high
concentrations to low concentrations?
Copyright © 2010 Ryan P. Murphy
• Answer! Kinetic movement of molecules causes
particles to move to open areas.
Copyright © 2010 Ryan P. Murphy

Molecules are trying to reach equilibrium.
Copyright © 2010 Ryan P. Murphy
• Activity! Making the room smell good.
– Smelling air freshener through the room.
(raise your hand when you smell it)
– What are the molecules doing?
Copyright © 2010 Ryan P. Murphy

Osmosis: The movement of water
through a semi-permeable membrane.
Copyright © 2010 Ryan P. Murphy
FACILITATED DIFFUSION
FACILITATED
DIFFUSION
Particles move from
a HIGH to LOW
concentration with
the HELP of a
PROTEIN.
They move WITH
the concentration
gradient
Check Point 2
1. What type of transport requires no energy and include
diffusion and osmosis?
2. What term is used to describe the diffusion of WATER
across a membrane?
3. What term is used to describe the movement of particles
across a membrane?
4. What term is used to describe the movement of particles
across a membrane with the help of a protein, but
without using energy?
How are they all related..?
Transport
Active Transport
Passive Transport
Diffusion
Osmosis
Facilitated Diffusion
What is ACTIVE TRANSPORT?
• A cell needs ENERGY to move particle across
the membrane.
• Particles are moving from an area of LOW
concentration to an area of HIGH
concentration
• That’s why the cell NEEDS ENERGY (going
AGAINST the concentration gradient)

Active transport
-
Copyright © 2010 Ryan P. Murphy

Movement of molecules from a less crowded to
a more crowded area.
Copyright © 2010 Ryan P. Murphy

Movement of molecules from a less crowded to
a more crowded area.
Copyright © 2010 Ryan P. Murphy

Requires the use of energy.
Copyright © 2010 Ryan P. Murphy

Proteins can do this.
What is PASSIVE TRANSPORT?
• Particles are moving from an area of HIGH
concentration to an area of LOW
concentration.
Passive vs. Active
TYPE
MOVES?
DIRECTION?
PASSIVE
ACTIVE
DIFFUSION
PARTICLES
High to Low
OSMOSIS
WATER
FACILITATED
DIFFUSION
ACTIVE
TRANSPORT
USES
ENERGY?
USES A
PROTEIN?
High to Low
NO
NO
NO
NO
PARTICLES
High to Low
NO
YES
PARTICLES
Low to High
YES
NO
DIFFUSION
DIFFUSION
Particles move from a
HIGH to LOW
concentration
They move WITH the
concentration gradient
OSMOSIS
OSMOSIS
WATER move from a
HIGH to LOW
concentration.
They move WITH the
concentration gradient
When does Passive Transport Stop?
• Passive transport will continue until
EQUILIBRIUM is reached.
• In other words, when there is the same
number of molecules on both sides
Check Point 3
1.
2.
3.
4.
5.
6.
What is a concentration gradient?
What is Active Transport?
Which direction does Active Transport move?
What is Passive Transport?
What direction does Passive Transport move?
What is Equilibrium?
HOW DOES THIS AFFECT THE CELL?
Water is LEAVING cell
Cell will SHRINK
No net movement
EQUILIBRIUM
Water is ENTERING cell
Cell will SWELL or BURST
HYPOTONIC
HYPERTONIC
ISOTONIC
This happens because the cell wants to balance out, if the particles cannot move the
Water must move to balance the concentration inside and outside of the cell.
REMEMBER: the water always moves from a HIGH to LOW concentration.

Hypotonic Solution: A solution that contains
less solute (more water) compared to the
cytoplasm of the cell.
Cell
Copyright © 2010 Ryan P. Murphy

Hypotonic Solution: A solution that contains
less solute (more water) compared to the
cytoplasm of the cell.

Water moves into the cell to equal out
concentrations.
Cell
Copyright © 2010 Ryan P. Murphy

Hypotonic Solution: A solution that contains
less solute (more water) compared to the
cytoplasm of the cell.

Water moves into the cell to equal out
concentrations. The cell swells
Cell
Copyright © 2010 Ryan P. Murphy

Hypotonic Solution: A solution that contains
less solute (more water) compared to the
cytoplasm of the cell.

Water moves into the cell to equal out
concentrations. The cell swells
Cell
Copyright © 2010 Ryan P. Murphy

Hypotonic Solution: A solution that contains
less solute (more water) compared to the
cytoplasm of the cell.

Water moves into the cell to equal out
concentrations. The cell swells
Cell
Copyright © 2010 Ryan P. Murphy

Hypotonic Solution: A solution that contains
less solute (more water) compared to the
cytoplasm of the cell.

Water moves into the cell to equal out
concentrations. The cell swells
Cell
Copyright © 2010 Ryan P. Murphy

Hypotonic Solution: A solution that contains
less solute (more water) compared to the
cytoplasm of the cell.

Water moves into the cell to equal out
concentrations. The cell swells
Cell
Copyright © 2010 Ryan P. Murphy

Hypotonic Solution: A solution that contains
less solute (more water) compared to the
cytoplasm of the cell.

Water moves into the cell to equal out
concentrations. The cell swells
Cell
Copyright © 2010 Ryan P. Murphy

Hypotonic Solution: A solution that contains
less solute (more water) compared to the
cytoplasm of the cell.

Water moves into the cell to equal out
concentrations. The cell swells
Animal cells
may
eventually
burst (lysis)
Cell
Copyright © 2010 Ryan P. Murphy
Cell will SHRINK
15% NaCL
85% H2O
ENVIRONMENT
CELL
5% NaCL
95% H2O
What is the direction of water movement?
copyright cmassengale
89
Cell at EQUILIBRIUM
10% NaCL
90% H2O
ENVIRONMENT
CELL
10% NaCL
90% H2O
NO NET
MOVEMENT
What is the direction of water movement?
equilibrium
The cell is at _______________.
copyright cmassengale
90
Cell will SWELL of BURST
10% NaCL
90% H2O
CELL
20% NaCL
80% H2O
What is the direction of water movement?
copyright cmassengale
91
Osmosis in Red Blood Cells
Isotonic
Hypotonic
copyright cmassengale
Hypertonic
92
1.COMPLETE STATION 1: ACTIVE VS.
PASSIVE TRANSPORT
• 2. COMPLETE STATION 2: PRACTICE WITH
TRANSPORT (HALF SHEET)
• 3. COMPLETE OSMOSIS PROBLEMS FOR
HOMEWORK
Concentration Gradient
What type of Transport?
DIFFUSION
OSMOSIS
ACTIVE
FACILITATED
MOVES PARTICLES
MOVES PARTICLES
MOVES HIGH TO LOW
MOVES HIGH TO LOW
USES A PROTEIN
NEEDS ATP
MOVES LOW TO HIGH
USES A PROTEIN
MOVES WATER
MOVES LOW TO HIGH
Three Forms of Transport Across the Membrane
copyright cmassengale
96
What will move?
• Water can move. Starch cannot.
What will move?
• Water can move. Salt cannot.
What will move?
• Carbon Dioxide Gas can move.
What will move?
• Salts are being moved using Active Transport!
PROTEIN
ATP
Exit Ticket
1. Which of the following correctly describes
diffusion?
a. It uses a protein and ATP energy
b. It uses a protein to move from a high to low
concentration
c. It moves water from high to low
d. It moves particles from high to low concentration
Data and Analysis
BEFORE
AFTER
1. Describe what happened.
2. Discuss diffusion and where in this experiment it occurred.
3. What part of the cell is responsible for maintaining homeostasis? What
material in this lab acted like this cell part?
Starch Diffusion Lab
• Purpose: We will observe how molecules
move from a high concentration to a low
concentration, through a membrane.
• Hypothesis: IF a bag with starch and water is
placed in a beaker of Iodine,
THEN_________________________________
_____________________________________
_
because______________________________.
Day 2:
Warm up:
Draw two cells. In once, show an example of
DIFFUSION and in the other show and
example of OSMOSIS!