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Personal well being
Well being of others in the lab.
The safety of equipment.
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First to call numbers
 Health Center
6666
▪
▪ 0-312-290 6666
 Security
1234
▪
▪ 0-312-290 1234
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Fire
General alb accidents
Chemical accidents
Optical accidents
Biological accidents
Nuclear accidents
Earthquakes
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Close doors and windows
Shut down air conditoning.
Sound the fire alarm
Notify (290) 1234
Secure injured
Notify (290) 6666 vya 1666 for injured
Attempt to extinguish the fire only if you are not in danger
yourself. Do so with a fire extinguisher.
 Early intervention with small fires can prevent
catastrophic big fires.
 Do you know how to use a fire extiguisher?
PECVD
Gas emergency Stop
Fire Alarm
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Shut down air conditioning in case of gaseous, or liquid
chemical contamination.
Notify authorities.
In case of splash on your garments, take them off
immediately. Wash affected area with running water for at
least 15 minutes.
Call 6666. Use acid neutralization kit.
If the area is safe, use eye wash or body wash showers.
Cleaning up is done with professional teams. But if you can
shut down the source of the contamination without putting
your self in danger, please do so.
Do you know how to use and oxygen cylinder or a flee mask?
First aid chemicals
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Know where the lab exit doors are.
Know the location and the use of safety showers.
Know the location of fire alarm buttons.
Know the location of the nearest phone.
Know the hazards of the materials and equipment you use.
Read MSDES files for the materials you use. Consult internet
for further info.
Do NOT work before taking necessary precautions.
Do NOT use materials you do not know and equipment you
are not trained for.
Consult M. Gure when necessary.
Those who are not approved by Murat Güre can not enter
class 100 lab.
Body and eye showers
Push Handle
Pull
MSDS files and flee mask
CLASS 100 escape doors
Fire Exits
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Do NOT enter the lab without gloves, gogoles, clean room
garmentsEldiven, gözlük, elbise gibi gerekli teçhizatı
kullanmadan laboratuvara girmeyiniz.
Do NOT eat, drink and smoke in the lab. You are discouraged
from wearing makeup in the lab.
Long hair must be tied in the back and taken into a hair net.
Drooping metal necklesses, arm bands or bracelets should
NOT be worn in the lab.
Short slacks are NOT allowed in the lab. Use a lab garments.
Do NOT use your mouth for pipette action .
After you leave the lab, wash your hands and check yourself
for possible chemicl splashes.
Do NOT use short cuts during the experiment. Use
equipment suitable for the task.
Mandatory equipment during use of chemicals
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Do NOT touch any equipment without the permission ofthe
user.
Food and drinks are stored in the refrigerator in the kitchen.
No chemicals in this refrigerator please.
NO food in the refrigerators in the lab.
If you use chemicals that takes too long to finish the job,
notify M. Gure the nature of the chemical process before
starting the experiments and get approval.
Read MSDS of cheimicals that you are using the first time.
Do NOT leave chemicals out in the open without marking
their identity and last use date.
Do NOT work alone in the lab during after hours. Notify the
security guard when wroking at night.
Working after midnight requires special written request by
your academic advisor from ARL Coordinator prior to any
work. Lab security is required to check that this is done.
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No jokes in the lab.
Leave the lab clean.Do NOT leave hazradous or flammable
materials out in the
Use minimum quantities of chemicals when working. Do
NOT store chemicals in your office or lab. Use chemical
storage area for excess chemical storage. See M. Gure for
this.
Please notify possible hazardous situations to lab personel.

Eye protection
 The use of glasses.
 The use of masks.
 The use of oxygen masks.
 The users of contact lenses. Beware!
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Respiratory protection
 Notify and get approval from M. Gure for experiments that
may release gasses requiring protection.
 Masks in the lab are flee masks. They should not be use for
long periods of time.
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Skin and body protection
 The use of gloves
 The use of lab coats and aprons
 The use of shoes.

Protection of hearing
Lanibar flow benches
Masks
Gloves
Body and eyes washes
Kits against chemical
contamination.
 Fire extinguishers
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Minimize hazards.
Safe use:
 Please also note the
properties of chemicals, alone
and in the reaction:
▪ What is the allowable
amount?
▪ What is the lethal or hurmful
dose?
▪ Possible ways to Chemical
exposure?
▪
▪
▪
▪
Contact with skin or eyes
Swallowing
Inhalation
Injection
 Type of safety threat?
▪ Corrosive
▪ Explosive
▪ Flammable
▪ Irritative
▪ Reactive
▪ Senstizer
▪ Poisonous
Safety symbols
(patlayıcı)
(oksitleyici)
(yanıcı)
(kanser yapıcı)
(zehirli)
(aşındırıcı)
(zararlı)
(tahriş edici)
 Type of safety hazard?
▪ Acute poisoning
▪ Carcinogen
▪ mutagenic
▪ Teratogenic
 Physical Properties Of Chemicals
▪ Aerosol
▪ Cryogenic
▪ Dust or particle
▪ Gas
▪ Liquide
▪ Solid
▪ Vapor density
▪ Vapor Pressure
▪ Flammability
 Chemical Compatibility
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Keep all chemicals closed and labeled.
If in the beaker don’t keep more than an hour.
Keep it at minimum amount if possible.
In Markup:
 Name of the chemicals
 Hazard Warning
 Name and tel. Of the user.
 Date.
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Do not store incompatible chemicals together.
Flammable Chemicals
 Flash point less then 38 C can be flammable.
 Flash point between 38-94C can burn.
Like:
 Benzene
 Alcohols
 Hydrogen sulfide
 Acetone
 Ethers
 Organic acids
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Corrosives
 Acids
▪ Oxidiser acids
▪
▪
▪
▪
Perchloric acid
Chromic acid
Nitric acid
Sulfric acid
▪ Mineral Acids
▪ Hydrochloric acid
▪ Hydrofloric acid
▪ Phosphoric acid
▪ Organic Acids
▪
▪
▪
▪
▪
▪
Acetic acid
Formic acid
Butyric acid
Propionic acid
Picric acid
Acrilic acid
 Keep away the acids from bases and active
metals. Like, potassium, magnesium.
 Keep away the acids from pissoning vapor
producer chemicals.
 Keep away the acids from tolune and xylene.
 Do not full up the bottles.
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Bases
 Reacts strongly with acids. They cause corrosion.
▪ Ammoniyum hidroksit
▪ Sodyum hidroksit
▪ Kalsiyum hidroksit
▪ Organik aminler
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Poisonous chemicals
 Should be kept in ventilated enclosures.
▪ Fosfin
▪ Arsin
▪ Osmiyum tetraoksit
▪ Dimetil sulfat
▪ Toluen 2,4-Diisosiyanat
▪ Sodyum azid
▪ 37% formaldahit
Separate oxidizing inorganic acids from organic
acids or flammable materials.
 Organic acids are flammable.
 Perchloric ascid ve picric acid requires special care. r.
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Reactive-Oxidizers
 Fire and explosion hazard
▪ Halogens
▪ Ammoniumpersulfate
▪ Hydrogen peroxide
▪ Sodium dichromate
▪ Potassium permanganate
▪ Perchloric acid
▪ Ammonium nitrate
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Reactive Reductants
▪
▪
▪
▪
▪
▪
▪
Ammonia
Carbon
Metals
Metalhydrades
Phosphorus
Silicon
Sulphur
 Keep away from the oxidisers.
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Water-reactive Chemicals
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Sodium
Potassium
Calcium carbide
Phosphoruspentachloride
Peroxide Producers
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Diethyl ether
Tetrahydrofuran
Acetaldehide
Isopropyl ether
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Avoid contact with eyes and skin. Do not use
liquid nitrogen without eye glasses or
protective mask . During the transportation
of LN use the protective gloves and eye
glasses to avoid skin touch and prevent the
face.
Equipment for handling liquid nitrogen
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Toxic
Flammable
 Phyrophoric
 Explosive
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Reactive
 Corrosive
 Oxidiser
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Choking
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High pressurized gases 100-2500 psig DANGEROUS!
Slow action to cylinders.
Do not move without covers!
The cylinder should be kept shackled to the wall!
Use proper ragulating valve.
Do not use damaged cylinders.
Label them as empty or full.
Threshold limit value - Time weighted average (TLVTWA): average exposure on the basis of a 8h/day,
40h/week work schedule
 Threshold limit value - Short-term exposure limit
(TLV-STEL): spot exposure for a duration of 15
minutes, that cannot be repeated more than 4 times
per day
 Threshold limit value - Ceiling limit (TLV-C):
absolute exposure limit that should not be exceeded
at any time
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 Take the chemicals safety. Valves, lasers, power
supplies should be closed.
 Depremde devrilecek malzeme olmamalı.
In Case Of Earthquake
 Please secure yourself first.
 Please do not leave the building during the
earthquake.
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Optical accidents:
 Eyes
 Skin
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High Voltage Sources
Current Sources
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-
Class 1:
Continuous watching harmless.
Packed as no one can reach.
Low-power lasers or high-power lasers in the indoor
environment.
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Class 2:
Normally harmless but during the long exposure it
would be harmful.
Power: < 1mw
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-
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Class 3a:
Harmless exposed to short term. But
dangerous if collimated.
Power: < 5mW (Laser pointers)
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Class 3b:
Direct or reftected exposure harmdfull but its
scttared light not as harmful.
Power: < 500mW
High power pointers
He-Ne lasers
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Class 4
Fully harmful
power >500mW
Eye and Skin damage. Fire causes.
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Eye or skin damage
Fire
Toxic gases in gas lasers
High Voltage
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Exposure to Skin or eye to the Laser
Direct exposure
Reflected light
(400nm – 1400nm) permanent damage to the
retina
.– Nd:YAG, Yb fiber, Cu-vapor, diode, HeNe,Ar+

Retinal damage more serious then cornea.
Savely use
Cornea damage
Retinal damage
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Fire hazard
Electrical circuits
Gases and flammable vapors
To solvents.
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Gas Hazards
Plastics!
Gas lasers gases

Lethal High Voltage
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Most of the accident!
ALTHOUGH power has been turned off the
shock!
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No metal goods on your body.
Do not stay on the wet floor.
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Protect your eyes, as appropriate.
Put the Lab entrance alert.
Put the optics low as much as possible.
Strong ambient lighting. So the narrow retina
diameter.
Keep airconditioners open.
Do not touch exposed cables, etc..
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While working in a laboratory security
ALWAYS should be kept at the forefront.
Otherwise your life (even worse of a friend)
can cost lives.
Please be careful!