UNIT 5
DAY 3
LAB RESULTS
EUKARYOTIC REGULATION
START LAB REPORT
OPENER
• In the lab, each group had four petri dishes,
labeled like this:
• +pGlo, LB/Amp
LB=Nutrients
• +pGlo, LB/Amp/Ara
Amp=Ampicillin
Ara=Arabinose
• -pGlo, LB
• -pGlo, LB/Amp
1. On which of the four plates do you expect to
see bacterial growth? Why?
2. On which plates do you expect to see bacteria
that glow under UV light? Why?
AGENDA
• Lab Results
• Eukaryotic Regulation vs. Prokaryotic
Regulation
• HOX genes
• Lab Report writing time
LAB RESULTS
In your notebook, answer the following questions
about EACH of the four petri dishes
1. Did bacteria grow on the plate?
2. How many colonies? (Estimate if necessary)
3. Does the bacteria glow when exposed to UV
light?
PROKARYOTIC VS.
EUKARYOTIC
REGULATION
WHAT SHOULD I DO?
1. MAKE A T-CHART
2. LIST THE STEPS OF EACH REGULATORY
PROCESS
3. THEN, GO BACK AND LOOK FOR SIMILARITIES
AND DIFFERENCES
THE LAC
OPERON
ANIMATION STYLE
SIMILARITIES?
DIFFERENCES?
QUESTION #1
WHAT ROLE DO
TRANSCRIPTION
FACTORS PLAY IN THE
REGULATION OF GENE
EXPRESSION?
QUESTION #2
WHAT ROLE DO HOX
GENES PLAY IN THE
REGULATION OF GENE
EXPRESSION?
Hox Gene
Gene 1
Gene 2
Gene 3
Gene 5
Gene 6
Gene 4
Gene 7
Gene 8
Gene 10
Gene 11
Gene 9
QUESTION #3
WHAT IS THE
CONNECTION BETWEEN
HOX GENES AND
TRANSCRIPTION
FACTORS?
Hox Gene
Gene 1
Gene 2
Gene 3
Gene 5
Gene 6
Gene 4
Gene 7
Gene 8
Gene 10
Gene 11
Gene 9
THE LAB REPORT
• 4 sections:
1. Purpose and background information
1.
2.
Bacterial Transformation
Operon Function
2. Procedure
3. Data
4. Analysis and conclusion
• You will be demonstrating your understanding of operon
function in TWO sections:
•
Purpose and background information: basic description of operon
function. To earn a 4, compare to eukaryotic regulation
•
Analysis and conclusion: explain your results, based on
understanding of operon function. To earn a 4, answer the
following question: “Could you do a similar experiment with
eukaryotic cells? Why or why not?”
WRITE
MULTIPLE
CHOICE?