S1
SYNTHESIS, CHARACTERIZATION, THERMAL AND
ANTIBACTERIAL STUDIES OF ORGANOTIN(IV) COMPLEXES OF
INDOLE-3-BUTYRIC ACID AND INDOLE-3-PROPIONIC ACID
Asha Chilwal1, Priti Malhotra2 and A. K. Narula1*
1
School of Basic and Applied Sciences, Guru Gobind Singh Indraprastha University, Dwarka,
Delhi 110078, India
2
Chemistry Department, Daulat Ram College, University of Delhi, Delhi 110007, India
Email: aknarula58@gmail.com
Supplemental Materials
Figure S 1 The IR spectra of organotin(IV) complexes: (a) Ph3SnIB (3), and (b) Ph3SnIP (6).
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Figure S 2 The IR spectra of organotin(IV) complex: Bu2SnIP2 (5).
Figure S 3 1H NMR for complex 3.
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Figure S 4 13C NMR for complex 3.
Figure S 5 119Sn NMR for complex 3.
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Determination of Antibacterial Activity
Chloramphenicol was used as standard drug. Using sterile microtitre plates, 0.2 mL of Mueller
Hinton Broth was added to each of the 96 wells. Doubling dilutions of each compound were made in the
wells, thus, a plate contained 0.5-100 µg mL-1 dilutions of six different complexes and of
chloramphenicol. In each plate one well was kept as positive control (broth + inoculum) and another as
negative control (broth only). The inoculum was adjusted to a turbidity equivalent to McFarland 0.5
turbidity standard. The inoculum was suitably diluted so as to get a final concentration of approximately
5×105 CFU mL-1 of bacteria in each well. Each well was inoculated with 0.01 mL of the prepared
inoculum using a multichannel micropipette and the plates were incubated overnight at 37°C. The MICs
of the organotin(IV) derivatives and chloramphenicol were determined by using the standard protocol of
NCCLS Broth Microdilution MIC method.
Table S 1 Antibacterial activity data for organotin(IV) complexes by MIC method in μgmL-1.
Bacteria
Compound
Standard drug
1
2
3
4
5
6
Chloramphenicol
Staphylococcus
aureus
50
28
20
55
32
16
70
Staphylococcus
epidermidis
50
28
16
60
32
24
70
Micrococcus
luteus
35
30
21
55
35
24
75
Escherichia
70
55
25
75
55
32
80
Pseudomonas
aeruginosa
70
55
30
75
55
32
85
Enterobacter
aerogenes
55
35
30
75
60
30
77
Coli