MICROBIOLOGY SYLLABUS
Tulsa Community College, Southeast Campus
Spring 2013
Biol 2164 Microbiology Section 307 and 308
Lecture 307 & 308: T&R 5:30 - 6:50 PM SE 8296
Lab: SE 8204
Section 307 Lab: T 7:00-9:50PM (call # 21540)
Section 308 Lab: R 7:00-9:50PM (call # 21544)
Hours of Credit: 4 semester hours
Instructor: Marva Volk
Office Room Number: SE 8281
Office Hours: T & R 11:00 PM - 4:00 PM
NOTE: I am usually in the Prep room next to lab
SE 8204 if I am not in my office
Science and Mathematics Division SE 8125
Office Hours: 8:00 AM - 5:00 PM
Associate Dean: Lyn Kent
Phone: 918-595-7742
Phone: 595-7687 Office (try this one first)
918-595-7958 Prep room (no messages)
Email: marva.volk@tulsacc.edu
COURSE PREREQUISITES
BIO1224 (Intro to Biology for Majors) or equivalent* AND CHE1114 (Principles of Chemistry) or CHE1315
(General Chemistry). This course will be taught with the assumption that you have a solid grasp of basic
chemical and biological concepts and terminology. *With approval of SEC Associate Dean of Math and Science.
COURSE DESCRIPTION
Included will be a survey of the viruses, protozoa and fungi of medical significance, with an in-depth survey of
bacteria, their morphology, metabolism, pathogenic capabilities and classification. Laboratory will emphasize
those techniques concerned with isolation and pure culture of microorganisms and the use of biochemical criteria
in taxonomy. Lecture 3 hours. Laboratory 3 hours.
TEXTBOOKS AND OTHER MATERIALS (See Blackboard for ISBN numbers)
REQUIRED: Microbiology, 3rd Edition, Robert W. Bauman, Benjamin Cummings Publishing Co.
A Photographic Atlas for the Microbiology Laboratory 4th Edition, Leboffe and Peirce. Morton Publishing
Lab Exercises for Microbiology 2164, TCC Southeast Campus,* Marva Volk, Neil Enis, Dusti Sloan, Ivan
Lurz,; Revised January 2013, TCC Publishing (Includes printed copies of Blackboard quizzes.)
Labcoat* (disposable), “sunglass style” safety goggles*, slides (1 package)*, non-latex gloves, colored pencils,
permanent marker (fine tip), large 3 ring binder for lab notebook and handouts. Coverslips will be provided.
*Best to purchase these items from the Southeast Campus bookstore.
SUPPLEMENTAL: USB flash drive (highly recommended); AIDS Update 2013, Stine, Prentice Hall
COURSE OBJECTIVES
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To provide the student with a thorough knowledge of the anatomy and physiology of microorganisms, with
emphasis on the bacteria.
To indicate bacterial diversity in a survey of the major orders of bacteria and the use of morphological and
physiological differences in establishing taxonomic schemes.
To survey physical and chemical techniques for microbial control, including sterilization, disinfection, and
chemotherapy.
To introduce the concept of pathogenesis and the resultant immune response in the infected host.
To provide a laboratory experience which will make the student proficient in culturing and identifying
bacteria by using staining, biochemical tests, selective and differential media, etc.
INCLEMENT WEATHER AND CLASS CANCELLATION POLICY
If severe weather is forecast, you should assume that TCC will be open unless you receive a TCC Alert. Signing
up for TCC Alerts is required; see TCC Homepage. TCC closings may also be announced on television and radio
stations. TCC is often not closed when the public schools are. However, your safety is of primary concern.
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INSTITUTIONAL STATEMENT, TOBACCO POLICY AND DISABILITY RESOURCES
Each student is responsible for being aware of the information contained in the TCC Catalog, TCC Student
Handbook, and Student Policies and Resources Handbook (on the TCC website), and semester information
listed in the Class Schedule and this Syllabus. In compliance with Oklahoma State law, Tulsa Community
College must maintain a Tobacco Free Environment. Tulsa Community College prohibits smoking or the use
of tobacco products on all property owned, leased or contracted for use by Tulsa Community College. It is the
policy and practice of Tulsa Community College to create inclusive learning environments. Accommodations for
qualifying students in compliance with the Americans with Disabilities Act (ADA) and Section 504 of the
Rehabilitation Act are available. To request accommodations, contact the Education Access Center (EAC) at
eac@tulsacc.edu or call (918) 595-7115 and advise the instructor of the accommodations needed at least a week
before a test or assignment date.
COMPUTER ASSISTED INSTRUCTION AND COMPUTER SERVICES ACCEPTABLE USE
Blackboard will be used for quizzes, announcements, project directions and other instructional materials. You
should check our Blackboard site DAILY and your TCC Outlook email often. For Blackboard and other internet
assignments, you may use the Math Lab SE 8150 (918-595-7751), the Computer Lab SEC 4231, the LRC, or you
may work from home if you have internet access. Access to computing resources is a privilege granted to all
TCC faculty, staff, and students. Use of TCC computing resources is limited to purposes related to the College’s
mission of education, research, and community service. See Student Policies and Resources Handbook.
LEARNING SUPPORT SERVICES
The SE Math Lab has printed copies of lecture notes and other material available in at the main desk for you to
copy. There are copies of our textbook and Bergey’s Manuals for use in the Math Lab (which you MAY NOT
copy) and in Micro lab and the LRC (which you MAY copy.) The SE Writing Center offers help building analytical
reading skills, solid research and effective writing skills and provides information on writing styles (SE 1102, call
918-595-7749 for appointment). Tutoring is free of charge to all TCC students.
ATTENDANCE, CELL PHONES AND OTHER ELECTRONIC DEVICES
ATTENDING LECTURES REGULARLY AND READING THE ASSIGNED TEXT AND LAB MATERIALS PRIOR
TO CLASS are among the most important factors in insuring your success in this class. Some materials covered
in lecture may not be presented in the textbook. You are responsible for all outside materials covered,
handouts, assignments, and announcements made during lecture. Information missed must be obtained
from the class website or your classmates. Excessive absences or failure to complete your unknown
identification, lab reports, and other assigned projects will lower your grade and may lead to administrative
withdrawal. Make sure you TURN OFF cellular phones, pagers and PDAs, etc., in class and lab. Text messaging
will lower your class attendance grade and will result in loss of credit for a lecture or lab exam. If you use a laptop
for editing/taking notes during lecture, email your notes to me right after lecture.
WITHDRAWAL, AUDIT AND INCOMPLETE POLICY
It is the student's responsibility to drop or withdraw by the scheduled deadline. Failure to withdraw may result in
the student receiving a grade of “F”. Non-attendance DOES NOT constitute official withdrawal; however excess
absence may result in administrative withdrawal. You must have a passing grade, instructor approval and
complete a contract to change from credit to audit or receive an incomplete. Contact the Counseling Office at any
TCC campus to initiate withdrawal from a course ('W' grade) or to change from Credit to Audit. See the
information about course withdrawal on Blackboard. The last day to withdraw with a “W” or change from
credit to audit is Friday, Nov. 9.
LABORATORY SAFETY
We will be working with live cultures in the laboratory. It is imperative that you follow all safety recommendations.
A lab coat is REQUIRED. You should wash your hands before and after each lab. Absolutely NO food or drink
is allowed in the laboratory. Please inform your instructor of any aspect of your health status (i.e. pregnancy,
medications, convulsions, etc.) which might impact your lab participation or performance.
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ACADEMIC DISHONESTY OR MISCONDUCT
Academic dishonesty or misconduct is not condoned or tolerated at campuses within the Tulsa Community
College system. Academic dishonesty is behavior in which a deliberately fraudulent misrepresentation is
employed in an attempt to gain undeserved intellectual credit, either for oneself or for another. Academic
misconduct is behavior that results in intellectual advantage by violating specific standard, but without deliberate
intent or use of fraudulent means. The student should review the relevant sections of the Student Policies and
Resources Handbook.
ACADEMIC ACCOUNTABILITY AND PLAGIARISM POLICY
Students will have opportunities for learning in both group settings and as individuals in this class. While I
encourage you to study with your classmates, you are expected to complete your own labs, reports and research
projects. There will be zero tolerance for academic dishonesty or misconduct or deliberate plagiarism. No credit
plus an additional penalty will be given for work that is not your own or work of your own that you allow to be
copied. A student guilty of academic dishonesty or misconduct or deliberate plagiarism may receive an “F” in the
course. “Plagiarism is claiming, indicating, or implying that the ideas, sentences, or words of another writer are
your own; it includes having another writer do work claimed to be your own, copying the work of another and
presenting it as your own, or following the work of another as a guide to ideas and expression that are then
presented as your own.” Review the Plagiarism information on Blackboard.
EVALUATION TECHNIQUES
There will be online and in-class or take-home quizzes covering material presented in lecture and lecture exams
(see below.) In-class group discussion quizzes will follow or precede most lectures. Each discussion group will
be composed of 3 - 4 students. Students in the group will use their lecture notes and text to answer the
discussion questions; all members present will receive the same grade. Absent members may earn partial credit
for one missed in-class quiz per lecture exam by submitting a make-up quiz on Blackboard before the lecture
exam. Make-up quiz forms and directions are posted in Assignments on Blackboard. Include your name, group
number, and date of quiz missed on your make-up assignments. The last two lecture exams will be given during
the final exam period. Material covered in lecture, on Blackboard quizzes and in reading assignments will be
covered on lecture tests. Class projects will include writing a report on a pathogenic microorganism and using
Bergey’s Manual. Late assignments will not be accepted without instructor approval. A lecture test may be made
up only if the instructor is notified prior to, if possible, or on the day of the exam. Make-up tests may have
additional written (essay, short answer, etc.) questions and must be completed prior to attending the next lecture.
Students who miss an exam will not be eligible to receive bonus or test curve points. If you have questions about
any grade received in this class, such as your grade for a test, assignment, lab, or report; you must speak with
and send a MyTCC email to your instructor within one week after receiving that grade and work with your
instructor to resolve the issue as soon as possible. Lab definitions and research questions in italics and bold
print are to be completed BEFORE coming to lab. Lab reports will be checked at the beginning of lab for prelab preparedness and labs reports may be due in lab at the end of the period the lab is completed. Lab quizzes
may be given in lab prior to or following the lab. Contact your instructor immediately if you miss lab to
arrange a make up. Due to the special materials needed, missed laboratory classes may be made up only with
the instructor’s approval. Excessive absences from lecture, lab and/or failure to complete your Unknown may
result in Administrative Withdrawal from the course. No electronic devices, cell phones, ball caps, dark glasses or
notes (unless specifically stated) during tests. *Note: Study aids and possibly extra credit will be available on
Mastering Microbiology but access to this site is NOT REQUIRED for this course.
EVALUATIONS
Possible Points
Grading Scale
Lecture Test 1-3, 80 points each, Test 4&5 (final), 160 points*
400 points
A: 900-1000 points
Blackboard Quizzes: (10 points/Exam)
50 points
B: 800-899 points
In-Class Quizzes (10 points/Lecture Exam)
50 points
C: 700-799 points
Class Participation and Attendance
50 points
D: 600-699 points
Bergey’s Project (25) & Disease Report Research Paper (75)
100 points
F: below 600 points
Laboratory Reports
200 points
Lab Exam
100 points
Unknown
50 points
Total Points:
1000 points
Extra Credit: Disease Presentation, additional Unknown documentation, seminars (see BlackBoard)
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RESEARCH ASSIGNMENTS
Bergey’s Project. 25 Points. This short project is designed to help you become more familiar with how to use
Bergey’s manuals, which you will use to help identify your Unknown. A form to use for your Bergey’s project and
a sign-up sheet will be available in lecture.
Written Disease Report. 75 Points.
Select a bacterial, protozoan, fungal, viral or prion disease. Look in Chapters 19-25 in our text, in Topley &
Wilson’s Microbiology and Microbial Infections (one of the excellent resources available on reserve in the
reference section in our LRC) or in the Bacterial Pathogens, Mycology, and Parasitic Protozoans Exercises in our
lab manual if you need some suggestions for your topic. Each student must choose a different disease for his or
her report. A sign-up sheet will be available in lecture. The same bacteria may, but does not have to, be used for
your Bergey's project and your disease report. If you choose to do your disease report on a disease not
associated with the bacteria used for your Bergey's project, you may “release it” so that another student may use
that topic for their report.
Review the information on Plagiarism on Blackboard and Handouts before you begin writing. Write a paper on
your topic. Include the classification of your organism in standard format (“Bergey Project” format for bacteria but
no Roman numerals required), in your paper. The text of your paper EXCLUDING title page information (title,
your name, class, date), section headings, spaces between paragraphs, illustrations, charts and any other
materials that you did not write should be at least five pages double spaced, with 1 inch margins, 12 point font
(Times New Roman, Arial or similar font.) You are allowed 3 lines for your classification information. Your paper
should be in the format of review article and may have a 1/4 - 1/3 page abstract. You should have five (or more)
references, cited in the format used for scientific journals (such as Nature), for your bibliography.
Your references should include:
1. A full text peer reviewed article, with references, from a refereed journal. For ONE peer-reviewed article, turn
in a copy of ONLY the title (first) page of the peer-reviewed article and one page of the bibliography
(usually last or next to last page of the paper).
2. One scientific journal article that is at least 2 pages long.
3. A recent journal article (~1 year). NOTE: One article may, but does not have to, fulfill requirements 1. - 3.
4. An article from the CDC website (see link on Blackboard in Projects&Reports). See me for an acceptable
alternative if needed.
5. Other references may include Topley & Wilson’s Microbiology and Microbial Infections (available in our LRC)
your text (if applicable) and Internet websites.
6. NOTE: You may NOT use Wikipedia as one of you five required references. If you do use Wikipedia, you
must correct the classification scheme to be consistent with Bergey’s Manual.
You must submit your report to SafeAssignment on Blackboard and receive a plagiarism score of 25% or less.
Directions for submitting your paper to SafeAssignment will be posted on Blackboard. You should post your
disease report file on the “Disease Report Parking Spot” in Reports&Projects on Blackboard for safekeeping.
Turn in a printed copy of your paper, with references, in lecture on the due date. Keep a copy of your paper to
use for your presentation (and as a back-up in case the Lost Assignments monster eats it.) The paper you turn in
will not be returned.
You will have the opportunity to make a ~ 10 minute presentation of your disease report for 10 points extra credit
when we cover diseases in lecture. Following the discussion of your disease presentation, you will present four
objective questions to the class and post your questions on our MyTCC "My Consolidated Courses" site. Exam
questions will be selected from student presentation questions.
Some Recommended Journals (there are many more excellent journals)
Journal of Cell and Molecular Biology
Journal of Microbiology Science News
Discover
Emerging Infectious Diseases
J. of Infectious Disease Clinical Infections Nature
New England Journal of Medicine
Journal of Bacteriology Scientific American Lancet
Virology
Cell
Science
Many of these journals are in the TCC LRCs. Feel free to use journals from other libraries. Many journal articles
are available online. If you use Internet resources, also include the web address in your bibliography.
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MICROBIOLOGY BIO 2164 307 & 308 TENTATIVE CLASS SCHEDULE 1-13-13 INSTRUCTOR: MARVA VOLK
NOTE: The class schedule may be changed AT ANY TIME by updates posted on Blackboard
or verbal notification in class
Week
LECTURE TOPICS (Tues. AND Thurs.)
LABORATORY (Tues. OR Thurs.)
All Blackboard Quizzes & Make-Up Quizzes must be
Date
Lab coat & Safety Glasses Required
submitted before the Lecture Exam unless other (Monday)
(for Labs 2-9)
specific directions are given.
1
Chapter 4: Microscopy
Blackboard and LRC Orientation. Meet in
Chapter 1: A Brief History of Microbiology
14-Jan Micro Lab 8204 then Computer Rm 8152
2
Chapter 2 The Chemistry of Microbiology
Lab 1 Microscope & Bacterial Morphology
(No Classes MLK Day, Mon. Jan. 21)
Chapter 3 Cell Structure and Function
21-Jan
3
Chapter 4: Staining and Classification
Lab 2 Aseptic Technique & Isolation Streak
Lecture Exam #1 Ch1-4
28-Jan
4
Chapter 5: Metabolism (omit photosynthesis,
Lab 3 Gram Stain
biosynthesis p. 149-158)
Chapter 6: Microbial Nutrition and Growth
4-Feb
5
Chapter 7: Microbial Genetics
Lab 4 Spore Stain & Acid Fast Stain
Transformation Lab Appendix A-D
11-Feb
6
Chapter 8: Recombinant DNA
Begin Unknown Journal: Part I. Streak, Ana Jar
RNA Interference (handout, website)
Lab 5 Negative Stains: Nigrosin & Capsule
Lecture Exam #2 Ch 5-8
18-Feb
7
Chapter 9: Controlling Microbial Growth
Begin Lab 6 Selective & Differential Media
Chapter 10: Antimicrobial Drugs
Unknown Journal: Part II. Unknown Gram
25-Feb Stain, Catalase & Oxidase tests, (Morphology)
8
Chapter 11: Prokaryotes
Complete Lab 6 Selective & Differential Media
Chapter 12: Eukaryotes
Unknown Morphology
Sign-up for Bergey Project
4-Mar Notebook Check: Turn in Labs 1-6
9
Chapter 13:Viruses
Lab 7 Inoculate Biochemical Tests; Biochem
Exam #3 Ch 9-13
Charts Due at BEGINNING of Lab
Sign-up for Disease Report
11-Mar
10
Spring Break No Lecture
Spring Break No Lab
18-Mar
11 Chapter 14: Infection & Epidemiology
Lab 7 Read & Turn In Biochemical Tests
Gloves are REQUIRED for this lab!
Chapter 15: Non-Specific Immunity
Bergey Project Due Thursday March 28
Inoculate broth for Lab 8 (begin working on Lab
25-Mar 8; prelab due next week)
12 Chapter 16: Specific Immunity
Lab 8 prelab due. Begin Lab 8 Effect of UV
Radiation&Antibiotic Sensitivity Testing
Work on Unknown Identification in Lab
1-Apr Begin working on your Lab 9 Charts!!!
13 Chapter 17: Immunization
Finish & turn in Lab 8 UV & Antibiotics
Begin Lab 9 Eukaryotic Microorganisms: Fungi
Chapter 18: Immunological Disorders
Disease Paper SafeAssignment Due Wed, April 10
Last Day to withdraw/audit: Fri. April 12
8-Apr
14
Disease Research Paper Due Tuesday April 18
Complete Lab 9 Eukaryotes: Protzoans
Turn in Lab 9 AND Lab 9 Chart
Chapter 19, Chapter 25, HIV, Disease Presentations
15-Apr Turn in Unknown Identification
15 Disease Presentations:
Lab Exam Review
Selected Diseases from Chapters 19 – 25
22-Apr
16
Blackboard Quizzes & Group Quiz Make-Ups for
Lab Exam
Ch. 14- 18 (T4) due Monday 4-29 11:55 PM
Disease Presentations
29-Apr
Tues. May 7 5:00-6:50pm Lecture Exam #4&5
No Lab
Ch 14-20 HIV 25, Diseases & BB Quiz Review
Questions from Ch 21 – 24
6-May
This syllabus may be altered or added to by the instructor at any time with a written, oral or Internet revision to the
students. Students are to abide by the TCC Student Code of Conduct in “Student Policies and Resources
Handbook.”
“I DO NOT GIVE GRADES. I RECORD YOUR ACHIEVEMENTS.”
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