AN OVERVIEW OF NUTRIENTS Nutrients Carbohydrates Proteins Lipids minerals vitamins water Dry Matter 1. Organic compounds: Carbohydrates Lipids Proteins Nucleic acids Vitamins Organic acids 2. Inorganic Compounds: - Minerals Proximate Analysis Moisture Ash Crude Protein Ether Extract Crude Fiber Nitrogen Free Extract (NFE): Calculated Proximate Analysis Dried Sample Kjeldahl (CP) Ether Extraction (EE) Fat Free Residue Boil in acid Ash Crude Fiber Boil in alkali Crude Fiber + Ash Crude Fiber Mono-gastric Animals …… Ruminant Animals …..? Detergents • NDF (cell wall contents): gut fill • ADF (NDF minus H-cellulose): digestibility • ADL ??? Carbohydrates Primary component of livestock feed Renewable resource as they are converted to CO2 and H2O. Primary carbohydrate in plants is glucose Comprise 70% of forage dry matter and 80% of concentrates Carbohydrates are a source of energy but there is no specific requirement for them Carbohydrates Carbon, hydrogen, oxygen Monosaccharide five pentose or six carbons hexose. Disaccharides, polysaccharides Starch Carbohydrates 1. Sugars: Mono (# C) : C3, C4, C5, C6, C7 Pentoses: Ribose; Hexoses: Glu, Gal, Man, and Fru Disaccharides: Sucrose, Lactose, Maltose Tri: Raffinose, Kestose Tetra: Stachyose Carbohydrates Polysaccharides NonSugars Homoglycans Pectic Substances Heteroglycans Arabinans Xylans Glucans Hemicellulose Complex Carbohydrates Gums Starch, Dextrin, Chondroitin Glycogen, Glycolipids Glycoproteins Cellulose Lipids General Soluble in ether Contain Carbon, Hydrogen and Oxygen C, H, O High proportion of carbon and hydrogen ~2.25 times the energy of carbohydrate per gram Lipids Classification Simple True fat - esters of glycerol Waxes - esters of other alcohols Classification Compound - groups other than alcohol and FA's Phospholipids - phosphoric acid and nitrogen Glyco-lipids - Carbohydrates and nitrogen Lipoproteins- proteins and lipids Lipids Glycerol-based Complex Simple Non Glycerol-based Phosphoglycerids Fat/ Oil Waxes Glycolipids Steroids Lecithin Cephalin Galactolipids Glucolipids Terpens Sphingomyelin Prostaglandins Lipids Classification Derived lipids Fatty acids Sterols Fats and Oils Fat vs Oil - Melting point only difference Fat = glycerol + 3 fatty acids Lipids Lipids Characteristics of fat due to type and proportion of different fatty acids Chain length Longer chains result in higher melting points. Butyric C4H8O2 Palmitic C16H32O2 Stearic C18H36O2 Examples: Fatty acids Oleic C18:1 CH3-(CH2)7-CH=CH-(CH2)7-COOH Linoleic C18:2 CH3-(CH2)4-(CH=CH-CH2)2(CH2)6-COOH Linolenic C18:3 CH3-CH2-(CH=CH-CH2)3(CH2)6-COOH Arachidonic C20:4 CH3(CH2)4(CH=CHCH2)4(CH2)2COOH Lipids Iodine number- A measure of un-saturation. Saponification number- A measure of chain length. The amount of alkali required to saponify 100 grams of fat. Other lipids: Sterols Cholesterol. Mainly in animal tissues. Hormones. Very little quantitatively. Ergosterol. A sterol in plants which can form vitamin D through the action of ultra violet light Other lipids Carotenes. Yellow colored substances common in plants that can be converted by animals to vitamin A. Phospholipids. Most abundant in blood, brain, liver and egg yolk. Can be utilized. β-carotene Phospholipids Other lipids Chlorophyll Green colored substance in plants - No feed value for animals. Waxes Surface of leaves and fruits. Essential oils. Give plants their odors and tastes. Proteins Long chains of Amino Acids. Contain Nitrogen, carbon, hydrogen, oxygen, and sometimes sulphur. Lose tertiary structure when heated. Proteins Globular Fibrous Conjugated Structural Transporter Catalytic Amino acids Traditionally Indispensable Dispensable Totally Indispensable C-skeleton Conditional Immaturity Newer Definition Human Nutrition Acquired dispensable Disorders AA intake Other N compounds Non protein Nitrogen (NPN) Urea Nucleic acids DNA RNA Additives Not as nutrients Some examples Antibiotics: growth stimulator/ disease control Anti-parasitic drugs Synthetic Antioxidants Antifungal agents Enzymes: improved digestion Digestion Primary Enzymes for Carbohydrates Food Source Enzyme Origin Product Starch, glycogen, dextrin Amylase Saliva & pancreas Maltose & Glucose Maltose Lactose Sucrose Maltase Lactase Sucrase • Ruminants do not secrete much sucrase • Inducible enzymes • Lactose intolerance SI SI SI Glucose Glucose & galactose Glucose & fructose Primary Enzymes for Proteins Food Source Enzyme Origin Product Milk protein Rennin Gastric mucosa Curd Proteins Pepsin Gastric mucosa Polypeptide Trypsin Chymotrypsin Pancreas Pancreas Peptides Peptides Carboxypeptidase Aminopeptidase Pancreas Small intestine Peptides & amino acids Polypeptides Peptides Primary Enzymes for Lipids Food Source Lipids Enzyme Origin Product Lipase & colipase Pancreas Monoglycerides & free fatty acids Nutrient Digestion - Lipids Large Lipid Droplet Action of bile salts Lipid emulsion Small Bile salts & pancreatic lipase and colipase Water soluble micelles Digestion of Lipid Bile salts emulsify lipids Pancreatic lipase acts on triglycerides Triglycerides acids sn-2 Monoglyceride + 2 fatty Pancreatic colipase Activated by trypsin Interacts with triglyceride and pancreatic lipase Displaces bile Improves activity of pancreatic lipase Importance of Pancreas for Digestion Produces enzymes responsible for 50% of carbohydrate digestion 50% of protein digestion 90% of lipid digestion Produces bicarbonate for neutralization of chyme in duodenum Digestive enzymes; summary