Be sure you have created an account. The Class Code is: 9268676
 Go to wikispace and click link to site: Fruit Fly Lab
 Do the following crosses (pay attention to Gender!) and be sure to record in your lab notebook each time! o You will do the Parental Cross to get the F1 generation - Be sure to check "Use in New Mating"; and "Add to Notebook" EACH TIME
 o Once that is done, you will do the F1 cross to get the F2 generation - "Add to Notebook" only
 Open your notebook to get the numbers
Add your data to class data (will be posted on Wiki later)
 Make a table for EACH CROSS and record your data. Example table is below:
Cross
Female parent’s phenotype
P
F1
F2
Wild (+)
Wild (+)
# female offspring:
Phenotype
Male parent’s phenotype
Vg
587: wild
(+)
429: wild
(+)
176: vg
Wild (+)
# male offspring:
Phenotype
596: wild (+)
436: wild (+)
158: vg
Keep in mind that YOUR TABLE MAY NEED TO HAVE ADDITIONAL COLUMNS
TO ACCOMODATE ALL OF THE VARIOUS PHENOTYPES!
Crosses to perform - if you do not finish in one day, you will need to split them up and each person will need to complete AND add info to the wiki page!
Cross Female Traits
#1
Male Traits
Wild Type - + (do not select ANY traits - just "Add to Shopping
Wing Size - Vestigial (vg)
Cart"
#2 Eye Color - White (w) Wild Type
#3
Body Color - Black (bl) AND Eye Color - Sepia (se) (yes, you can select 2 traits at once!)
Wild Type
#4
#5
#6
#7
Wild Type
Wing Shape - Dumpy (dp)
Body Color - Yellow (y) AND Eye Color - Brown (bw)
Wild Type
Body Color - Ebony (e) AND Eye
Color - Sepia (se)
Wing Angle - Dichaete (d)
Wild Type
Eye Shape - Bar (b)
Created by: Jennifer Forsyth, Woodstock High School

Go back to your page of fly data. You will do a Chi-Square Analysis of the following crosses ONLY!
Cross #1, #2, #3, #6, and #7
Observed # Expected # (o - e)
2
/e
BEFORE you do the analysis, you must do a punnett square to determine the expected ratios of your F2
flies!
THEN, total your flies and find the expected. If you do not remember how we did this in class, ASK
ME!!!!
Note: For the first Chi-Square analysis, do NOT separate out your males/females - just do it by trait. It may be necessary to separate out later (see below).
You are under the assumption that all traits were 1) simple dominant/recessive inheritance, 2) on separate chromosomes if a dihybrid cross and 3) not sex-linked. Now, at LEAST 2 of your crosses will NOT support your hypothesis for the cross (hypothesis was either just monohybrid or dihybrid). In that case, look at your males/females and see if there are discrepancies - that will tell you if it's sex-linked. If it is dihybrid and not working, you may be looking at either 1) a normal chromosome and sex-linked chromosome OR 2) linked genes on a non-sex chromosome. REDO your Punnett Squares for these crosses and REDO the chi-square analysis to see if the new expected numbers are correct. DO NOT remove your original tables - just add another one.
Created by: Jennifer Forsyth, Woodstock High School