Austrian monk, born 1822, who carried out work with various plants that contributed to the understanding of genetics Most notable of the plants was his work with pea plants Fertilization: production of a new cell True-breeding: plants that, if allowed to self-pollinate, would produce offspring identical to the parents Mendel cross-pollinated the pea plants by removing the male reproductive parts and dusting pollen from another plant onto the flower This produced seeds with two different parents Mendel was able to study the results of different characteristics these plants had Genes and Dominance Mendel studied seven different pea plant traits Trait: specific characteristic (seed color, height, etc.) that varies from one individual to another Mendel crossed seven contrasting characteristics and studied the results Hybrids: offspring of crosses between parents with different traits 1. Biological inheritance is determined by factors that are passed from one generation to the next 2. Principle of dominance – some alleles are dominant and others are recessive Alleles: different forms of a gene A recessive allele will only be evident when a dominant allele is not present Chromosome Number Basics.. = each chromosome from the male parent has a corresponding chromosome from the female parent (a match!) A cell that contains both sets of homologous chromosomes is said to be diploid. The number of chromosomes in a diploid cell is sometimes represented by the symbol 2N. For Drosophila, the diploid number is 8, which can be written as 2N=8. Chromosome Number Basics.. The gametes of sexually reproducing organisms contain only a single set of chromosomes, and therefore only a single set of genes. These cells are haploid. Haploid cells are represented by the symbol N. For Drosophila, the haploid number is 4, which can be written as N=4. Meiosis is a process of reduction division in which the # of chromosomes per cell is cut in half through the separation of homologous chromosomes in a diploid cell Meiosis involves two divisions, meiosis I and meiosis II. By the end of meiosis II, the diploid cell that entered meiosis has become 4 haploid cells (gametes). Copyright Pearson Prentice Hall Phases of Meiosis I Meiosis I Interphase I Meiosis I Prophase I Metaphase I Anaphase I Telophase I and Cytokinesis Prophase I (Meiosis I) Chromosome replication occurs during interphase Tetrad – formed when a chromosome pairs with its homologous chromosome There are 4 chromatids in a tetrad. When homologous chromosomes form tetrads in meiosis I, they swap portions of their chromatids in a process called crossing-over. Crossing-over produces new combinations of alleles. Copyright Pearson Prentice Hall Spindle fibers attach to the chromosomes, which are lined up in the middle of the cell Copyright Pearson Prentice Hall The fibers pull the homologous chromosomes toward opposite ends of the cell. Copyright Pearson Prentice Hall Nuclear membranes form The cell separates into two cells End result of Meiosis I: two cells that have chromosomes and alleles different from each other The two cells produced by meiosis I now enter a second meiotic division. Unlike meiosis I, neither cell goes through chromosome replication. Meiosis II Telophase I and Cytokinesis I Meiosis II Prophase II Metaphase II Copyright Pearson Prentice Hall Anaphase II Telophase II and Cytokinesis Nuclear Membrane dissolves Spindle forms Just like old times! Copyright Pearson Prentice Hall The chromosomes line up in the center of cell. Copyright Pearson Prentice Hall The sister chromatids separate and move toward opposite ends of the cell. Copyright Pearson Prentice Hall Meiosis II results in four haploid (N) daughter cells. Copyright Pearson Prentice Hall Gamete Formation Meiosis produces 4 genetically different haploid cells In male animals, meiosis results in four equal-sized gametes called sperm. Copyright Pearson Prentice Hall In many female animals, only one egg results from meiosis. The other three cells, called polar bodies, are usually not involved in reproduction. Copyright Pearson Prentice Hall Questions! (Page 278) 1. 4 haploid cells genetically different from one another and the original cell 2. Mitosis produces two genetically identical diploid cells; meiosis produce for genetically different haploid cells 3. Diploid: two sets of chromosomes Haploid: one set of chromosomes 4. Homologous chromosomes pair up and form tetrads, which may exchange portions of their chromatids results in the exchange of alleles between the homologous chromosomes 5. Both sperm and egg cells have 23 chromosomes because they are gametes, which are haploid cells. A white blood cells has 46 chromosomes because it is a diploid body cell Questions! (page 266) 1. Dominant: form of an allele who trait always shows up if it is present. Recessive: form of an allele whose trait shows up only when the dominant allele is not present 2. Separation of paired alleles Alleles are separated during gamete formation with the result that each gamete carries only a single allele from the original pair 3. Factors that are passed from one generation to the next 4. Mendel cut away the male parts of one flower; then dusted it with pollen from another flower 5. Only ¼ of the possible gamete formations did not have a dominant allele 6. True-breeding pea plants have two identical alleles for one gene, so in a genetic cross each parent contributed only one form of a gene, making inheritance patterns more detectable Mitosis results in the production of two genetically identical diploid cells. Meiosis produces four genetically different haploid cells. Copyright Pearson Prentice Hall Comparing Mitosis and Meiosis Mitosis Cells produced by mitosis have the same number of chromosomes and alleles as the original cell. Mitosis allows an organism to grow and replace cells. Some organisms reproduce asexually by mitosis. Copyright Pearson Prentice Hall Comparing Mitosis and Meiosis Meiosis Cells produced by meiosis have half the number of chromosomes as the parent cell. These cells are genetically different Meiosis is how sexually reproducing organisms produce gametes. Copyright Pearson Prentice Hall Parent F1 F2 •Parent (P) generation = the first 2 individuals that mate in a genetic cross •F1 generation = first offspring from a cross between the (P) generation •F2 generation = offspring from crosses among individuals of the F1 generation Genotype: A set of alleles that determines the expression of a particular characteristic or trait (MMRr) Phenotype: physical characteristics (tall, green, etc.) Homozygous dominant: MM (two capital); dominant trait expressed Heterozygous dominant: Mm (one capital, one lowercase); dominant trait expressed Homozygous recessive: mm (two lowercase); recessive trait expressed Eye color: Brown = dominant BB, Bb Blue, green, hazel = recessive bb Facial Features Freckles = dominant FF, Ff No freckles = recessive ff Dimples = dominant DD, Dd No dimples = dd Hair Color Dark hair = dominant HH, Hh Blonde hair = recessive hh What does the symbol 2N represent? What is our haploid number? How many cells does meiosis result in? Are these cells haploid or diploid cells? What are the female haploid cells created during meiosis? 6. What are the male haploid cells created during meiosis? 7. During what specific phase of meiosis do tetrads form? 8. What process during meiosis creates genetic variance across generations? 1. 2. 3. 4. 5. Genotype Punnett Squares Diagram that shows the result of a genetic cross Simple Punnett Square – one trait examined Two generations shown Letters represent alleles (genotype) Parent Generation F1 Generation Breakdown of cross results Let’s cross two of the offspring from the F1 generation genes that segregate independently do not influence each other’s inheritance Dihybrid Cross • Cross between 2 heterozygous dominant individuals crossed • 2 traits crossed • 16 box square The phenotype ratio predicted for dihybrid cross is 9:3:3:1. 9 offspring that exhibit both dominant traits 3 offspring that have one dominant, one recessive trait 3 offspring that have one dominant, one recessive (opposite of last) 1 offspring that is recessive for both traits Rr(Yy) take the first R and multiply it out with the two Y’s Ry RY Rr(Yy) rY ry now focus on the next r and multiply it out by the two Y’s Set up the Punnett Square with the allele combinations you got from the FOIL method. Begin to cross the parents to create the new generations If one parent is homozygous recessive, and other parent is homozygous dominant = F1 will be heterozygous dominant If crossing two heterozygous dominant, ratio will always be Break down genotype into Trait Lab Widows Peak Bent Little Finger Hitchhiker’s thumb Tongue Rolling Tongue Folding Dimple Chin Long Eyelashes Free Ear Lobe Three traits examined 64 box Punnett Square Ratio of a cross 27 : 9 : 9 : 9 : 3 : 3 : 3 : 1 27 = all dominant 9 = 2 dominant, 1 recessive 3 = 1 dominant, 2 recessive 1 = recessive heterozygous phenotype is somewhere in between the two homozygous phenotypes •Example: red, white, and pink flowers Codominance: situation in which both alleles contribute to the phenotype Example: black and white chicken feathers “erminette” Appear separately, not as a “blend” Multiple alleles: genes that have more than two alleles Example: blood type DOES NOT MEAN the individual can have more than two alleles (hh, AA, Aa) Type A – has the A antigen on red cells Type B – has the B antigen on red cells Type AB – has both A and B antigens on red cells Type O – has neither A nor B antigens on red cells Rh (rhesus) is a protein found on the surface of red blood cells. is a single gene with two alleles, “+” and “-” Rh+ is dominant Rh- is recessive Named after the monkeys used to study the blood types Caucasians African American Hispanic Asian O+ 37% 47% 53% 39% O- 8% 4% 4% 1% A+ 33% 24% 29% 27% A- 7% 2% 2% 0.5% B+ 9% 18% 9% 25% B- 2% 1% 1% 0.4% AB + 3% 4% 2% 7% AB - 1% 0.3% 0.2% 0.1% To prevent this condition, she can take a drug that keeps her from developing the antibodies to the Rh+ blood group Range of eye color caused by particular combinations of alleles Locus (plural loci) is the specific location of a gene Eye color is found on different loci Alleles B/b and G/g Come together and give eye color Two eye colors Heterochromia Considered abnormal and may be pathological Born with two different colored eyes congenital heterochromia Height # of Fingers (d) Poison ivy susceptibility (R) Karyotype A set of photographs of chromosomes grouped in homologous pairs Used to analyze chromosomes 46 (diploid) chromosomes in humans Sperm (haploid) = 23 chromosomes Egg (haploid) = 23 chromosomes Pairs 1 through 22 = Autosomes 23rd pair = Sex Chromosomes Sex linkage is the phenotypic expression of an allele related to the chromosomal sex of the individual. Different from autosomal trait inheritance, where both sexes have the same probability of inheritance. In mammals: female is the homogametic sex, with two X chromosomes male is heterogametic, with one X and one Y chromosome A male or female child of a heterozygous mother affected with an X-Linked dominant trait has a chance of inheriting the mutation children of an affected father will be inherit the affected X chromosome (daughters possess their fathers' X-chromosome). children of an affected father will be affected (sons do not inherit their fathers' Xchromosome). Because the Y-chromosome is small and does not contain many genes, few traits are Y-linked, and Ylinked diseases are rare Since the only humans who have a Y chromosome are males, Y-linked traits are passed only from father to son X-Linked Genes Linked to the (no kidding?) No male will get an X-linked trait from his father, only from his mom Mom’s have 2 X’s to donate 50% probability of inheritance for males and females Y-Linked Genes Linked to the Small chromosome, not many traits Passed from father to son Examples of X and Y Linked Traits X-linked Hemophilia Rickets (dominant) bone deformity Rett Syndrome growth failure, small hands, feet, head Male pattern baldness (recessive) Colorblindness Y-linked Retinitis pigmentosa damaged retina, vision impairment Azoospermia immobility of the sperm or inability to produce sperm •RECESSIVE TRAIT Mendel’s 3 Laws Law of Dominance – he figured out that some traits are dominant, some are recessive Law of Independent Assortment – alleles separate independently during meiosis Homologous chromosomes lining up in the middle of the cell varies from cell to cell because there’s NO SET WAY they have to arrange. Law of Segregation – the two copies of genes present in an individual’s cells segregate during the formation of gametes (mom gives one set, dad gives another). Polygenic Traits Polygenic traits are controlled by two or more than two genes at different loci on different chromosomes. These genes are described as polygenes “poly” means many! Examples are human height, weight, skin color Eye color is a polygenic trait with multiple alleles. Probability and Mendelian Genetics Probability is the likelihood that an event will occur When using Punnett Squares, you are showing the probability (likelihood) of certain traits appearing in offspring with 2 parents are crossed Punnett Squares don’t show actual results – they show possible results. If enough is known about the family genetics, then Punnett Squares are much more accurate. methemoglobinemia Chart that shows the relationships within a family Family tree! Used to determine genotypes of family members for certain traits Interpreting a Pedigree Chart 1. Determine if the pedigree chart shows an autosomal or X-linked disease: If mostly males in the pedigree are affected the disorder is X-linked because males have only one X and therefore cannot be a carrier no dominant allele to cover up the recessive If it is a 50/50 ratio between men and women the disorder is autosomal. Is it Autosomal or X-linked? Autosomal – roughly 50/50 male and female X-linked Trait – males seen with it 2. Determine whether the disorder is dominant or recessive: If the disorder is dominant, one of the parents must have the disorder AND the disorder will be seen across all the generations If the disorder is recessive, neither parent has to have the disorder because they can be heterozygous AND the trait will NOT be seen across all generations Meiosis: 1 diploid cell 4 haploid cells 1 cell with 46 chromosomes 2 cells with 46 chromosomes 4 cells with 23 chromosomes 2 DIVISIONS! (meiosis I and meiosis II) Mitosis: 1 diploid cell 2 diploid cells 1 cell with 46 chromosomes 2 cells with 46 chromosomes Male and Female Gametes 23