Lab Safety for Dissections 1. Safety goggles must be worn at all times because the preservative is very harmful to your eyes. If you are injured immediately notify the teacher. No horseplay or running around the room. Be careful because scissors are sharp. Absolutely no body parts leave the room. Dispose of them properly. Be sure to clean up and wash hands when you are done. No vomit in Stew’s room!! 2. 3. 4. 5. 6. 7. Earth Worm Dissection 1. Insert the point of your scissors about 1 cm posterior to the clitellum of the worm just to the left of center on the dorsal side. Be sure the scissors are just under the skin. Carefully cut through the skin to the mouth. Using a dissecting needle, sever the muscles between the segments, opening up the worm. Use pins to attach the skin to the dissecting tray. Find the organs listed in your notes. Research their functions for your dissection journal. 2. 3. Materials 1. 2. 3. 4. 5. 6. Tray Two paper towels Gloves Scissors Pins Goggles Worm organs Brain: Is located in the nervous system and is the control center of the body. It is located under the pharynx. Aortic arches: It has five of them and is another word for heart. Crop: It is used to store food. Gizzard: It is used to grind up organic food and it is the area of the digestive tube which is quite muscular. Intestine: It extends, relatively unchanged, from about segment 20 to the posterior end of the worm. Dorsal blood vessel: The dorsal blood vessel is used to carry blood from the tail to the head of the worm. Seminal vesicles: They are used to store sperm which are produced by the worm. Seminal receptacles: These are only visible when the worm is in reproductive condition. Esophagus: It is a muscular tube that is used to take food and water from the mouth to the stomach. It serves as a passageway for food. Mouth: The mouth chews up the food and makes it easier to digest. Anus: It is used to excrete liquid and solid wastes from the body. Clitellum: An organ that is responsible for mucus production during reproduction. Skin: It is used to absorb oxygen. Crayfish dissection procedure 1. 2. 3. Observe the external anatomy of the crayfish. Locate the body parts that are external. Is your crayfish a male or a female? How do you know this? Locate the mouth of the crayfish. Observe the live crayfish in the tank. Make a cut in the exoskeleton of the cephalothorax on the dorsal side from the back of the cephalothorax to the rostrum, cutting down the midline. Separate the 2 halves of the exoskeleton to reveal the internal organs. After you find all the internal organs clean up thoroughly! Materials 1. 2. 3. 4. 5. 6. 7. Tray Two paper towels Gloves Scissors Probe Goggles Pins Fish dissection procedure 1. 2. Identify all the fins of the fish. Find the lateral line, operculum and gills. Make a cut from the vent on the ventral side of the fish to an area just below the operculum, cutting between the pelvic fins. Then cut up along the operculum to the lateral line. Cut parallel to the lateral line until you are even with the vent, then cut down to the vent and remove the side of the fish. Locate the internal organs and then clean up thoroughly. Fish organs Operculum: A lid or flaps covering an opening, such as the gill cover in some fish. Stomach: It is used to store and digest food. Intestine: Liver: Heart: It is responsible for pumping blood through the body. Swim bladder: Eye: Ovaries: Testes: It is used to produce gametes. Caudal fin: Dorsal fin: Pectoral fin: Ventral fin: Pelvic fin: Lateral line: Scales: Frog dissection questions 1. How many chambers does a frog heart have? Three chambers 2. How many chambers does a human heart have? Two chambers 3. Which heart is more efficient, and why does it have to be more efficient? A frog’s heart is more efficient and it has to because in order for it to jump and catch insects with its tongue. 4. Why does a frog have such small lungs? A frog has such small lungs because 5. How is the tongue of a frog attached in its mouth and why is it attached like this. The tongue of a frog is attached in its mouth in the front because that way it can extend out further so it can catch different types of insects at an farther distance. 6. Why is a frog classified as the following? a. ectotherm b. amphibian c. chordate d. animal e. heterotroph 7. Why are amphibian populations declining, and what can we do to save them? Amphibian populations are declining because of pollution, hunting them, and killing of them just for the fun of it. What we can do to save them is 8. Explain how a frog is camouflaged. A frog is camouflaged because if it was on to-p of the water and there was another animal that was looking up to the surface of the water they would just see yellow which looks like the sun. 9. Is a frog an herbivore, omnivore or carnivore? Explain your answer. 10. Why do scientists dissect organisms? The reason why scientists dissect organisms is to find out what kind of food they eat and more information about them that they didn’t know. Materials 1. 2. 3. 4. 5. 6. 7. Tray Two paper towels Gloves Scissors Probe Goggles 10 pins Frog organs Skin: It is used for camouflage and protection. Lungs: It is used to function so it can remove the carbon dioxide from the blood and provide it with oxygen. Heart: It is responsible for pumping blood through the body. Brain: Is located in the nervous system and is the control center of the body. Ovaries: Testes: It is used to produce gametes. Tongue: It is used to catch insects. Cloaca: Spleen: It is used to dispose of red blood cells. Pancreas: Stomach: It is used to digest food. Small intestine: It is used to serve to digest and absorb nutrients. Large intestine: It is used to absorb water from and eliminate the residues of digestion. Liver: Gall bladder: Kidney: Bladder: Fat Bodies: