State of Israel / Ministry of Agriculture and Rural Development ARO – Agricultural Research Organization Breeding and engineering nonfood crops for biodiesel Yedidya Gafni – Institute of Plant Sciences Agricultural Research Organization (VOLCANI Center) P.O.B. 6, Bet-Dagan, 50250 Israel Tel: +972-3-9683111 / Fax: +972-3-9665327 Using biomass for energy Bio-Fuels • Type of Renewable Energy (derived from the Sun). • Biofuels are liquid fuels made from esters, alcohols, ethers, and other biomass chemicals (Source: US Dept. of Energy). • Common biofuels include: ethanol and biodiesel. • Ethanol is made from starches / sugars. • Biodiesel is an ester made from vegetable oils, animal fats or other types of biomass. Biodiesel (bio-petroleum diesel) • B100 = 100% biodiesel / B20 = 20% / B5 = 5% / B2 = 2% • Production process: Transesterification (of oil) • Main crops used for producing biodiesel: • • • • • • Rapeseed (Europe) Soybean (US) Jatropha (Africa, India) Castor Bean (Africa, China, S. America) Palm Oil (Tropical areas) More • Other oil-enriched crops: Coconut, Brazil nuts, Jojoba, Peanuts, Cotton seed, Sunflower, and many more. Schematic of the Transesterification process BIODIESEL – Final Product Biodiesel 100% Glycerin BioDiesel is here From the Farmer to the Fuel Tank Oilseed Meal Farming Energy Crop R&D Crushing Biodiesel Biodiesel Production Glycerin Market Crop Oil BioDiesel – A better fuel vs. Diesel Features Higher cetane Greater lubricity Superior detergency Higher flash point Benefits More mileage Greater horsepower Less smoke Smoother running engines Quicker starts Longer engine life Reduced maintenance Biodiesel Advantages • Produced from renewable materials – eco friendly / closed CO2 cycle. • Local & self production – less reliance on foreign oil. • Contains practically no sulfur (0.001%) – non toxic. • Considerably decreases emissions (up to 50%). • Easily decomposes – does not harm soil or ground water. • Biodiesel is not hazardous material (flashpoint above 110°C). • Eligible as fuels under international standards & specifications (world-wide). • Eligible for CDM (Clean Development Mechanism - Kyoto Treaty). • More… Clean Development Mechanism (CDM) – Carbon Credits (CER) “.. the mechanism of the Kyoto Treaty (CDM) is now in force to reduce industrial & commercial greenhouse gas emissions – planting of bio-fuel crops may well create carbon sinks that can earn cash through their sale of emissions credits to polluting industries in developed countries.” The potential of acquiring GHG reductions, in the form of CER, for bio-diesel projects is about 3 tons of CO reduction per year (3 CER) for every ton of petroleum replaced. 2 Current price for 1 CER is about US$ 5 - 7. Biodiesel Drawbacks • Biodiesel can be corrosive to rubber materials. • Biodiesel is not necessarily more economic than regular diesel. Depend on the plants, their related processes and, other incentives. • Biodiesel can last up to 6 months. In 6-12 months need to be treated. Biodiesel Refineries / Plants (EU) 75% of total biodiesel production belong to 4 corporations: ADM (Archer Daniels Midland), Cargill, Bunge & Saipol US – Existing BD Plants US Biodiesel Market US Biodiesel Consumption US Biodiesel Plants: 65 operating plants (capacity: 400M gal/yr) 50 - construction (capacity: 700M gal/yr) US Biodiesel Production US Biodiesel Production Gallons (millions) (Source: National Biodiesel Board, Apr. 2006) 400 350 300 250 200 150 100 50 0 1999 2000 2001 2002 2003 2004 2005 2006 2007 Proj Proj The EU Biodiesel Market Total European Biodiesel Market: Unit Shipment and Revenue Forecasts (Europe), 1997-2007 Units (Million Tonnes) Revenues ($ Million) 5 3000 4.5 2500 4 3 2.5 1500 2 1000 1.5 1 500 0.5 0 0 1997 1998 1999 2000 2001 2002 2003 2004 Year Source: European Biodiesel Board, 2006 2005 2006 2007 Units (Million Tonnes) Revenues ($ Million) 3.5 2000 Projections: 2006 – 5B L/yr 2010 – 12B L/yr 2020 – 25B L/yr World Production of Biodiesel 2005 - 2010 US – 18% 75% Source: Biodiesel 2020 Global Market Survey, Oct. 2006 2005 Western Europe (D, FR, IT, UK, etc.) – main producers and consumers – 75% of world Eastern Europe & N. America – 2nd largest markets Asia – although significant market yet, relatively small 38% 30% 2010 Western Europe (D, FR, IT, UK) – main producers and consumers – 38% of world Asia – will become the 2nd largest market (China, India – consumption & production) Eastern Europe & N. America – 3rd largest markets, with US as the single largest consumer – 18% of world Biodiesel – Crops & Yields Crop Kg oil/ha Liters oil/ha US gal/acre Oil Content Castor beans 1188 1413 151 50% - 55% Coconut 2260 2689 287 70% Corn (maize) 145 172 18 12% Cotton 273 325 35 13% - 15% Jatropha 1590 1892 202 30% - 35% Palm oil 5000 5950 635 35% Peanuts 890 1059 113 36% Rapeseed 1000 1190 127 37% Soybean 375 446 48 15% Sunflower 800 952 102 32% Source: www.journeyforever.com – Jan. 2007 – This data is compiled from a wide variety of sources. The yield figures are most useful as comparative estimates, crop yields vary widely. ARO & Biodiesel The ARO (also known as ‘The VOLCANI Center‘) aims at becoming the 'ultimate knowledge base' regarding complete Seed – to – Harvest processes of two selected plant types: Jatropha curcas and Castor bean • Jatropha curcas small shrub native of C. America and a member of the Euphorbia family, it is a drought-resistant perennial, living up to 50 years and growing on marginal soils. • Oil content: ~35% • Oil: Not Edible. • Other oil uses: None • Castor bean - droughtresistant, annual plant of tropical origin with immense spread-out. The plant can grow between 2 to 5 m. • Oil: soluble in alcohol (no heat) – fit for biodiesel • Oil content: ~55% • Oil: Not Edible (high level of Ricinoleic Acid – 85%). • Other oil uses: Over 700 The Research Team Prof. Yedidya Gafni – Head of Team. In charge of developing tissue culture & transformation protocols. Dr. Yiftach Vaknin – In charge of the Jatropha curcas project. Dr. Oren Ostersetzer – In charge of the Castor bean project. Dr. Samuel Gan-Mor – In charge of developing mechanical harvesting machines. Prof. Dani Shteinberg – In charge of the studies of plant diseases. Dr. Asher Bar-Tal – In charge of the studies of soil chemistry and plant nutrition and fertilization. ARO's 'Biodiesel Plants – Package of Knowledge' include ICM (Integrated Crop Management) & complete research on the following process: Jatropha curcas Superior Varieties Development Complete Cultivation Process Genetics & Breeding -------Seed / Tissues Water & Soil Technologies -------Growth (cultivation) Harvest & Post Harvest IP Protection Complete Field Support -------Seeds Growth Yield Harvest ... Phytopathology & Entomology -------Growth (cultivation) Mechanization Engineering -------Harvest, Collection & Post-Harvest Castor bean Superior Varieties Development Complete Cultivation Process Harvest & Post Harvest IP Protection Complete Field Support -------Seeds Growth Yield Harvest ... ARO – Advantages for BD Crops • Unique & patented (PBR) varieties with superior characteristics for: – Fast growth rate. – Better yields. – Increased oil content (in the seeds). – Regulated fruition and harvest (opposed to wild, un-regulated fruition). – Lower toxicity of Castor bean plants (the oil is non-toxic). – Complete match to various climates, soil type & composition, water, etc. – Pre-determined plant's shape & size – suitable for mechanical harvesting. – High resistance to pests & diseases. • Development of mechanical harvesting machinery. • Complete professional support and guidance in cultivation of Pilot Plantation – at any location of potential project. Jatropha as living hedge in Ethiopia 158.987296 Castor varieties in our collection Ornamental Castor plant What can genetic engineering contribute? • Make plants give higher oil yields for making biodiesel • Enhance the oxidative stability of the oil • Render plants resistant to biotic stress • Resistance to viruses (ACMV?), fungi, bacterial diseases, nematodes and insects and more. • Render plants resistant to abiotic stress • Salinity, drought, heat, cold marginal soils and more. •Control plants height for mechanical harvesting Regeneration of Jatropha curcas in tissue culture Thank You 158.987296