OF NIRUPMA VED Contact Information Nirupma ved Email: vaidnirupama@gmail.com Ph: +91-9907093302, Nirupma VED Email:vaidnirupama@gmail.com Experience in Research : More than 6 years experience of research in reputed Research Institutes (Indian Veterinary research UniversityGwalior) InstituteMukteswar campus, National Dairy Research Experience in Teaching: Professional profile: Skills: Proficient with isolation of nucleic acid from blood, body fluids, tissues Institute- Karnal, Jiwaji More than 2.5 years teaching experience inboth colleges(Bsc,Msc students) and school (Sophia College, Gwalior; Subhash Chandra Bose College, Gwalior and Navodaya Vidhyalaya, Pichhore, Dabra, (M.P) As a biotechnologist have extensive experience in laboratory techniques, able to successfully apply broad knowledge base in modern molecular biology to novel research challenges . and clinical samples, PCR, RTPCR, Real time PCR, , cultivation and isolation of viruses, cell culture, development of enzyme immunoassays, viral protein characterization, sequencing,reporting research findings. Academic Record Qualifications School/College/University University/ Year Board CANCER HOSPITAL AND RESEARCH Jiwaji uni 2011INSTITUTE Jiwaji uni Gwalior(M.P. Gwalior UGC Regulation 2009 SCBC College Gwalior Jiwaji 2001-2003 M.Sc.(Biotechnology) Universiy Gwalior Bundelkhand 1998-2001 B.Sc.(Zoology,chemistry,Botany D.V.C. College, Orai University, ) Jhansi G.G.I.C., Orai U.P. Board 1996-1998 Intermediate (Allahabad) D.A.V. College, Orai U.P. Board 1996 Higher Secondary (Allahabad Ph.D Biotechnology Marks (%) Submitted in Dec 2014 68.0% 58% 51% 60% Ph.D is submitted in DEC 2014 with course work and according to UGC regulation ( 2009). PhD TOPIC: “MOLECULAR CHARACTERISATION AND GENOTYPING OF HUMAN AND ANIMAL ROTAVIRUES ISOLATES FROM NORTHERN INDIA” Employment Record Name of organization Position held, with Brief resume of duties date National Institute of Scientist B july 31 Worked in BSL2,3 lab ….. virology Pune (MH) 2014 –oct 7 2014 National institute of high Senior. Research fellow, security animal disease 20May2013- 7July 2014 laboratory, BHOPAL (BSL-3)LAB Research on Project entitled-“Surveillence of Avain Influenza Programme in india”. 1.Inoculation, Isolation and cultivation of viruses . 2.HA-HI test 3.Diagnostic PCR 4.Cloning and Sequencing 5.Data collection ,Report writing Bioinformatics Anlaysis. Indian Veterinary Senior Research Fellow, Dec’08- 18 june 2012 Research Institute,Mukteswar campus Research in NAIP (World Bank) funded project on “Toll-like receptors in Farm animals and Applications in Evolutionary Lineages in Disease Resistance” Institution Project On “Prevalence characterization and development of Indigenous Diagnostics for Animal Rotaviruses” Report writing, Findings, Data collection ,Bioinformatics analysis, Primer designing. Teaching TO Intermediate students . Navodaya Vidhyalaya,, PGT in Biotechnology, SEPT 2006 to MARCH Pichhore, Dabra,(M.P) 2007 Lecturer in Biotech Teaching TO (PG&UG) Sophia College, Gwalior Department, AUG2005 to FEB 2006 Subhash Chandra Bose Lecturer, Sept.2003 to TeachingTO (PG&UG) July 2004 College, Gwalior Research Experience at Master’s Degree Master’s Research: “Cloning of Ovine Inhibin Gene in a Suitable cloning vector” Master’s Guide: Dr. S. De, Senior Scientist, Genomics Lab,Animal Biotechnology center National Dairy Research Institute- Karnal Industrial Training One month Training at Sanchi Dugdh Sangh Banmore, Gwalior. Conducted test on Adulteration in various milk products. Conference\workshop\Training\ symposium attended Participated in International biosafety Awareness Programme Including Workshop On Culture of Responsibility on January 11, 2014 at High Security Animal Disease Laboratory,IVRI,Bhopal (M.P.) Training cum workshop on 'Conventional and Molecular techniques for diagnosis of trans-boundary animal diseases', Phase 1: 18-25 February, 2014; Phase 2: 28 Feb - 7 March, 2014 at HSADL, IVRI, Bhopal Participated in ICAR-Sponsored short-term training under Niche Area of Excellence on” RECENT ADVANCES IN DISEASE DIAGNOSIS OF LIVESTOCK” Organized at IVRI ,Campus Mukteswar from Feb 22-March 2,2012 Participated in 3 days Hands on Training in” TECHNIQUES IN PROTEOMICS” Jointly Organized by College of Life sciences gwalior and Helini Biomolecules ,Chennai during May 5-7,2011. Attended National Symposium on” FRONTIERS IN BIOCHEMISTRY AND BIOTECHNOLOGY” held on March 16-17,2012 organised by school of studies in Biochemistry and Biotechnology, Jiwaji university Gwalior(Madhya Pradesh) Participated in workshop on” Molecular & Immunological methods”(ELISA FLOW Cytometry,PCR,RFLP,RAPD and Microarrays) Sponsored by Society for Immunology and Immunopathology at Central jalma Institute for leprosy and other Mycobacterial Diseases(ICMR)Agra ,Feb 15,2004 MEMBER OF SOCIETY Life member of National science congress Publication in peer reviewed journals (9) INTERNATIONAL (2014) Chakradhar Tosh, S Nagarajan, PhD; H V Murugkar, PhD; M Kumar, PhD; M Pandey, M.V.Sc.; D K Jolhe, Ph.D.; S Tripathi, M.Sc.; S Shukla, M.Sc.; N Vaid, M.Sc.; A Mishra, M.Sc.; R C Ghosh, Ph.D.; D D Kulkarni, Ph.D.” Emergence of M2 Val27Ala mutation associated with amantadine-resistance in clade 2.3.2.1 H5N1 highly pathogenic avian influenza virus in India” Research in Veterinary Science(communicated Feb 3) Y.P.S. Malik, V. Rawat, K. Sharma, N. Kumar, Nirupama vaid,, R. Rakholia, and B. Ganesh “Molecular evidence of Multiple Viral Infections in an Infant Hospitilised with Acute Gastroenteritis in India” Journal of Pure and Applied Microbiology,Feb 2014 Vol.8(1)p.733-737 (2013) “YASHPAL SINGH MALIK1, KULDEEP SHARMA2, NAVEEN KUMAR3, ADIL A HAQ4 and NIRUPMA VAID “Determination of bovine rotavirus genotypes (G and P) circulating inUttarakhand (2010–2012) by polymerase chain reaction” Indian Journal of Animal Sciences 84 (2): 42–00, February 2014/Article “Yashpal S. Malik*, Kuldeep Sharma, Nirupama Vaid, Munish Batra, Minakshi Prasad, Gaya Prasad, Baldev R. Gulati, and Kiran N. Bhilegaonkar “Designing and validation of new primers for effi cient genotyping of animal G3 rotaviruses” VETERINARSKI ARHIV 83 (6), 611-622, 2013 (2012) Malik YPS, Sharma K, Vaid N, Chakravarti S, Chandrashekar KM, Basera SS, Singh R, Minakshi, G. Prasad, B.R. Gulati, KN Bhilegaonkar, and A.B. Pandey (2012).” Frequency of group A rotavirus mixed G and P genotypes in bovines: Predominance of G3 genotype and its emergence in combination with G8/G10 types”. Journal of Veterinary Sciences(International) JOURNAL OF VETERINARY SCIENCES. (2012), 13(3), 271-278 (2011) YPS Malik, D Singh, KM Chandrashekar, S Shukla, K Sharma, N Vaid and S Chakravarti (2011). “Occurrence of dual infection of peste -des petits-ruminants and goat pox in indigenous goats of central India, phylogenetically relatedness of PPR virus with Tibet and Chinese strains”. Transboundary and Emerging Diseases (PMID: 21235732: published).Page no-1-6(International) 2011 Jun;58(3):268-73. doi: 10.1111/j.1865-1682.2011.01201.x. Epub 2011 Jan 16 YPS Malik, S. Chakravarti, K. Sharma, N. Vaid, K. K. Rajak, V. Balamurgan, S. Biswas, B. Mondal, R.S. Kataria and R.K. Singh. (2011). “Genomic analyses of Toll-like receptor 4 and 7 exons of Bos indicus from temperate subHimalayan region of India”. Asian Australasian Journal of Animal Sciences vol.24.no.7: Page no 10191025(International) YPS Malik, KM Chandrashekar, K. Sharma, Adil Haq, N Vaid, S. Chakravarti, M Batra and AB Pandey (2011). Picobirnavirus detection in buffalo calves suffering from acute diarrhoea. Tropical Animal Health Production 21 March 2011 DOI: 10.1007/s11250-011-9834-0.Pageno.1475-1478(International) NATIONAL (2010) S. S. Basera, R. Singh, N. Vaid, K. Sharma, S. Chakravarti and Y P S Malik (2010). “Detection of Rotavirus infection in bovine calves by RNA-PAGE and RT-PCR”. Indian Journal of Virology page no-144-147(National) Abstracts in National/ International Conferences 2014 C.TOSH, S.Nagarajan,H.V.Murugkar,M.Kumar,S.Shukla,S.Tripathi,N.Ved,A.Mishra and D.D.Kulkarni.Isolation and genetic characterisation of avain influenza(sub-type H5N1) viruses Isolated from chicken,duck and crow in Odisha,2012 and 2014 at 2nd Annual conference and National Symposium on “Integrating poultry Health and Food Safety” AAHP-2014 7-8Nov 2014 Pune India. 2012 Z. Nongothung Ezung, Rashmi Singh Kuldeep Sharm, Naveen Kumar, Amit Kumar Nirupama Vaid and Yashpal S. Malik Molecular typing of the structural genes of bovine and human group A Rotavirus at virocon 2012 Nov 8-10 Indian Vetrinary Research institute Muktewar campus Naveen Kumar, Kuldeep Sharma, Amit Kumar Sharma, Nirupama Vaid, Subhankar Sircar and Y.P.S. Malik Expression and purification of polyhistidine-tagged truncated NSP-4 protein of rotavirus E2 genotype in prokaryotic expression system at virocon 2012 Nov 8-10 Indian Vetrinary Research institute Muktewar campus Adil Anamul Haq, Y.P.S. Malik, Kuldeep Sharma, Nirupama Vaid and S. Chakarverdi Development and evaluation of real time PCR assay for rapid diagnosis of bovine group A rotaviruses at virocon 2012 Nov 8-10Indian Vetrinary Research institute Muktewar campus Adil Anamul Haq, Y.P.S. Malik, Kuldeep Sharma, Nirupama Vaid and S. Chakarverdi Determination of P and G genotypes in bovine rotaviruses: A need to update the genotyping primers at virocon 2012 Nov 8-10 Indian Vetrinary Research institute Muktewar campus Amit Kumar Sharma, Kuldeep Sharma, Naveen Kumar, Nirupama Vaid and Y.P.S. Malik Bovine Picobirnaviruses: Detection, phylogenetic analysis and development of RdRp gene based RT-PCR diagnostic assay at virocon 2012 Nov 8-10Indian veterinary Research institute Muktewar campus 2011 Poster presentation Malik YPS, Chakravarti S, Vaid N, Sharma K, Sen A, Venkatesan G, Bhilegaonkar KN and AB Pandey (2011). Development of rapid nucleodiagnostic assay for detection for rotaviruses: Assessment of clinical significance in farm animals. In: X Agriculture Science Congress on “Soil. Plant and Animal health for Enhanced and sustained Agricultural Productivity” held at National Bureau of Fish Genetic Resources, Lucknow from10-12 Feb 2011. Yashpal .S .Malik, Kuldeep sharma .Nirupama vaid ,S.chakravarti ,Naveen kumar and AB Pandey(2011)Development and Evaluation of real-time Multiplex RT-PCR for detection and NSP4 gene based genotyping of group A Rotavirus Virocon 2011 Yashpal .S .Malik, Kuldeep sharma. Nirupama vaid, Rashmi singh &Awadh B Pandey(2011) Occurrence of group A rotavirus mixed G and p genotypes in Bovines: Predominance of G3 genotype in Combination with G8\g10 types Virocon 2011 2010 Malik YPS, Chakravarti S, Vaid N, Sharma K, Kataria RS, Raj GD,Singh RK and AB Pandey (2010). Genetic Characterization and Evolutionary Lineages Analysis of TLR2 and TLR4 of Bos grunniens (Yak) from North Eastern region, India. In: XVII Annual Convention of Indian Society of Veterinary Immunology & Biotechnology held at Department of Veterinary Microbiology & Biotechnology, College of Veterinary & Animal Science, Rajasthan University of Veterinary & Animal Sciences, Bikaner-334001, Rajasthan. Malik YPS, Chakravarti S, Vaid N, Sharma K, Singh RK and Bhanuprakash V and AB Pandey (2010). Molecular characterization and evolutionary lineage analysis of Toll like Receptor gene 7 of Bos grunniens (Yak) and Bos indicus of Uttarakhand, India. In: 5th Uttarakhand State Science & Technology Congress held at Doon University, Dehradun, Uttarakhand from 10th to 12th November 2010. S. Chakaravarti, S.K. Biswas, K. Sharma, N. Vaid, R.S. Kataria, G.D. Raj, V. Bhanuprakash, R.K. Singh, B. Mondal and YPS Malik. Characterization of Toll-like Receptor 4 and 7 exon fragments from Bos indicus of Kumaon (Hill) region, Uttarakhand. In 10th Indian Veterinary Congress, XVIIth Annual Conference of IAAVR and National Symposium on Newer Challenges in Veterinary Research and Education vis-à-vis Safe Animal, Food and Human Health” held from 11-12 March 2010 at College of Veterinary Science & Animal Husbandry, Jabalpur-482 001 (M.P.). YPS Malik, S Chakaravarti, SK Biswas, K Sharma, N Vaid, RS Kataria, GD Raj, V Bhanuprakash, B Mondal and RK Singh. Genetic characterization and evolutionary lineage analysis of Toll-like receptor 7 of Bos grunniens (Yak) from Himalayan region of India. In XIX National Conference of VIROCON on “Recent trends in viral disease problems and management” held at Dept. Of Virology, Sri Venkateswara University, Trupati- 517502, Andhra Pradesh, from 18-20 March 2010. 2009(1) Malik YPS, Vaid N, Chakravarti S, Sharma K, Biswas SK, Mondal B, Balamurgan V, Singh RK and Bhanuprakash V. Genetic characterization and evolutionary lineage analysis of Toll Like Receptor gene 7 of Bos indicus from Hill region of Kumaon, Uttarakhand. In: 4th Uttarakhand State Science & Technology Congress held at GB Pant Agricultural University, Pantnagar, Uttarakhand from 10th to 12th November 2009, p325. Book/Manual Chapter contribution (3) 2012 Y.P.S.Malik,K sharma,N.Yadav,N.Vaid,and Amit kumar Approches in the Diagnosis of Rotavirus Infection, Indian Veterinary Research Institute Mukteswar,Nainital(uttarakhand) 2011 Malik YPS, Chakravarti S, Sharma K, Vaid N and A.B.Pandey (2011). Toll like innate immune receptors: Potential Clinical Applications. In: ICAR sponsored 3 day workshop under NAIP project (Component 4) entitled “Toll-like receptors in Farm animals and Applications in Evolutionary Lineages in Disease Resistance” on “Real-time PCR and SNP analysis” from 2-4th Feb,2011 at Dept. of Animal Biotechnology, Madras Veterinary College, TANUVAS, Chennai. 2010 Chakravarti S, Shrivastava S, Malik YPS, Vaid N, Sharma K, Haq AA and Pandey AB (2010). TLR agonsits: Novel prospective for developing smarter vaccines. In: Applied Aspects of Biotechnology, Eds. Shrivastava S, Sonal and Kumar S, Indian Veterinary Research Institute, Izatnagar 243122 (UP), India, pp 229-237. Technical/Popular Article (1) S Chakarvarti, N Vaid, K Sharma, SK Biswas, YPS Malik, B Mondal and V Bhanuprakash (2009). Amplification and cloning of full length Toll-like receptor 7 gene of Bos indicus from hilly terrains of Kumaon region, Uttarakhand. IVRI News letter. 31( 2):3-4 ; July-December, 2009 Nucleotide NCBI GenBank Submission Rotavirus Chakravarti, S., Vaid, N., Sharma, K., Venkatesan, G., Sen, A., Singh, R., Bhilegaonkar, K.N., Bhanuprakash, V. and Malik, Y.P.S. Bovine group A rotavirus strain Kumaon (UKD) 01 VP6 gene, partial cds (GU592182) Chakravarti, S., Sharma, K., Vaid, N., Mohapatra, J.K., Pandey, L.K., De, A., Venkatesan, G., Sen, A., Bhanuprakash, V., Bhilegaonkar, K.N. and Malik, Y.P.S. Bovine rotavirus strain Bov/Ind/UKD/09/M-1(RV-A) VP7 gene, partial cds (HM235505) Malik, Y.P.S., Chakravarti, S., Sharma, K., Vaid, N., Venkatesan, G., Sen, A., Mohapatra, J.K., Bhilegaonkar, K.N., Pandey, L.K., De, A and Bhanuprakash, V. Bovine rotavirus strain Bov/Ind/UKD/09/M-1(RV-A) VP4 gene, partial cds (HM235506). Chakravarti, S., Vaid, N., Sharma, K., Sen, A., Venkatesan, G., Mohapatra, J.K., Pandey, L.K., De, A., Bhilegaonkar, K.N. and Malik, Y.P.S. Bovine rotavirus strain Bov/Ind/UKD/09/M-1(RV-A)VP6 gene, partial cds (HM235507). Malik, Y.P.S., Chakravarti, S., Sharma, K., Vaid, N., Sen, A., Venkatesan, G., Mohapatra, J.K., Pandey, L.K., De, A., Bhilegaonkar, K.N. and Pandey, A.B. (2010). Bovine rotavirus strain Bov/Ind/UKD/IVRI/09/M-1(RV-A) VP6 inner capsid gene, complete cds (HM235508). Chakravarti, S., Vaid, N., Sharma, K., Venkatesan, G., Sen, A., Mohapatra, J.K., Pandey, L.K., De, A., Bhilegaonkar, K.N., Pandey, A.B. and Malik, Y.P.S. (2010). Rotavirus strain Hu/Ind/UP/09/BE-1(RV-A) NSP4 enterotoxin gene, complete cds (HM235509) Malik, Y.P.S., Chandrashekar, K.M., Chakravarti, S., Sharma, K. and Vaid, N. Rotavirus strain Buffalo/Ind/MP/08/B46(RV-A) VP7 gene, complete cds (HM235510). Malik, Y.P.S., Chandrashekar, K.M., Chakravarti, S., Kadam, M. and Vaid, N. Rotavirus strain Buffalo/Ind/MP/08/B48 (RV-A) VP7 outer capsid gene, partial cds (HM235511). Malik, Y.P.S., Chandrashekar, K.M., Kadam, M., Chakravarti, S., Sharma, K. and Vaid, N. Rotavirus strain Buffalo/Ind/MP/08/B-68(RV-A) VP7 gene, partial cds (HM235512) Malik, Y.P.S., Chandrashekar, K.M., Kadam, M., Sharma, K., Vaid, N. and Chakravarti, S. Bovine group A rotavirus Bov/Ind/MP/08/C-32(RV-A) VP7 gene, partial cds (HM355547) Malik, Y.P.S., Chandrashekar, K.M., Kadam, M., Chakravarti, S., Vaid, N. and Sharma, K. Rotavirus group A Hu/Ind/MP/08/H-14 VP7 outer capsid protein gene, partial cds (HM355548) Malik, Y.P.S., Chandrashekar, K.M., Kadam, M., Sharma, K., Vaid, N. and Chakravarti, S. Rotavirus group A Hu/Ind/MP/08/H-16 VP7 gene, partial cds (HM355549) Malik, Y.P.S., Chakravarti, S., Sharma, K., Vaid, N., Sen, A., Venketesan, G., Mohapatra, J.K., Pandey, L.K., De, A., Bhilegaonkar, K.N., Bhanuprakash, V. and Pandey, A.B. Bovine rotavirus A isolate Bov/Ind/UKD/IVRI/09/M-1 Non Structural Protein 1(NSP1)gene (A11 genotype), complete cds (HM363560) Malik, Y.P.S., Chakravarti, S., Vaid, N., Sharma, K., Sen, A.,Venketesan, G., Mohapatra, J.K., Pandey, L.K., De, A., Bhilegaonkar, K.N., Bhanuprakash, V. and Pandey, A.B. Bovine rotavirus A isolate Bov/Ind/UKD/IVRI/09/M-1 Non Structural Protein 2 (NSP2) gene (N2 genotype), complete cds (HM363561) Malik, Y.P.S., Chakravarti, S., Sharma, K., Vaid, N., Sen, A.,Venketesan, G., Mohapatra, J.K., Pandey, L.K., De, A., Bhilegaonkar, K.N., Bhanuprakash, V. and Pandey, A.B. Bovine rotavirus A isolate Bov/Ind/UKD/IVRI/09/M-1 Non Structural Protein 3 (NSP3)gene (T6 genotype), complete cds (HM363562) Malik, Y.P.S., Chakravarti, S., Vaid, N., Sharma, K., Sen, A.,Venketesan, G., Mohapatra, J.K., Pandey, L.K., De, A., Bhilegaonkar, K.N., Bhanuprakash, V. and Pandey, A.B. Bovine rotavirus A isolate Bov/Ind/UKD/IVRI/09/M-1 Non Structural Protein 4(NSP4)gene (E2 genotype), complete cds (HM363563). Malik, Y.P.S., Chakravarti, S., Vaid, N., Sharma, K., Sen, A., Venketesan, G., Mohapatra, J.K., Pandey, L.K., De, A., Bhilegaonkar, K.N., Bhanuprakash, V. and Pandey, A.B. Bovine rotavirus A isolate Bov/Ind/UKD/IVRI/09/M-1 Non Structural Protein 5 (NSP5)gene (H3 genotype), complete cds (HM363564) Malik, Y.P.S., Chakravarti, S., Sharma, K., Vaid, N., Sen, A., Venketesan, G., Mohapatra, J.K., Pandey, L.K., De, A., Bhilegaonkar, K.N. and Pandey, A.B. Bovine rotavirus strain Bov/Ind/UP/10/ME-8 (RV-A) VP7 gene, partial cds (HM567170) Malik, Y.P.S., Chandrashekar, K. M., Chakravarti, S., Sharma, K., Vaid, N., Mohapatra, J. K., Pandey, L. K., De, A., Venketesan, G., Sen, A., Bhilegaonkar, K. N. and Pandey, A.B. Bovine rotavirus A isolate Bov/Ind/MP/08/B-47 Non Structural Protein 4(NSP4)gene (E2 genotype), complete cds (HM591492) Malik, Y.P.S., Chandrashekar, K.M., Chakravarti, S., Vaid, N., Sharma, K., Sen, A., Venketesan, G., Mohapatra, J.K., Pandey, L.K., De, A., Bhilegaonkar, K.N. and Pandey, A. B. Bovine rotavirus A isolate Bov/Ind/MP/08/B-48 Non Structural Protein 4 (NSP4) gene (E2 genotype), complete cds (HM591493) Malik, Y.P.S., Chandrashekar, K.M., Chakravarti, S., Sharma, K., Vaid, N., Venketesan, G., Sen, A., Mohapatra, J.K., Pandey, L.K., De, A., Bhilegaonkar, K.N. and Pandey, A.B. Bovine rotavirus A isolate Bov/Ind/MP/08/B-100 Non Structural Protein 4 (NSP4) gene (E2 genotype), complete cds (HM591494) Malik, Y.P.S., Chandrashekar, K.M., Chakravarti, S., Vaid, N., Sharma, K., Sen, A., Venketesan, G., Mohapatra, J.K., Pandey, L.K., De, A., Bhilegaonkar, K.N. and Pandey, A.B. Bovine rotavirus A isolate Bov/Ind/MP/08/B-72 outer capsid VP7 glycosylated protein gene (G10 genotype), partial cds (HM591495) Chakravarti, S., Basera, S.S., Vaid, N., Sharma, K., Venketesan, G., Sen, A., Mohapatra, J.K., Pandey, L.K., De, A., Bhilegaonkar, K.N., Pandey, A.B., Madan, R.S. and Malik, Y.P.S. Bovine rotavirus A isolate Bov/Ind/UKD/PTN/09/P-43 Outer Capsid VP7 Structural Protein gene (G6 genotype), partial cds (HM591496 ) Malik YPS, Chakravarti S, Vaid N, Sharma K, Batra M, Sen A, Bhilegaonkar KN, Bhanuprakash V, Venketesan G and Pandey AB (2010). Bovine rotavirus A isolate Bov/Ind/UKD/PTN/09/P-14 Non-structural Protein gene (NSP4 E2 genotype), partial cds. (HQ157344). Malik YPS, Chakravarti S, Prasad M, Sharma K, Vaid N, Prasad G, Sen A, Venketesan G, Bhilegaonkar KN and Pandey AB (2010). Bovine rotavirus A isolate Bov/Ind/HAR/BRV-133 Non-Structural Protein gene (NSP4 E2 genotype), partial cds (HQ157345). Malik YPS, Chakravarti S, Chandrashekar KM, Vaid N, Sharma K, Sen A and Pandey AB (2010). Bovine rotavirus A isolate Bov/Ind/MP/JBP/08/B-72 Non-Structural Protein gene 4 (E2 genotype), partial cds (HQ157346). Chakravarti S, Malik YPS, Sharma K, Vaid N, Bhilegaonkar KN, Sen A, Venketesan G and Pandey AB (2010). Bovine rotavirus A isolate Bov/Ind/UP/10/ME-8 Non-Structural Protein gene 4 (E2 genotype), partial cds (HQ157347). Malik YPS, Chakravarti S, Basera S, Sharma K, Vaid N, Singh R, Bhilegaonkar KN and Pandey AB. 2010. Bovine rotavirus A isolate Bov/Ind/UKD/PTN/09/P-970 Non-Structural Protein gene 4 (E2 genotype), partial cds (HQ157348). Malik YPS, Minakshi P, Chakravarti S, Vaid N, Sharma K, Sen A, Venketesan G, Pandey AB and Prasad G. 2010. Bovine rotavirus A isolate Bov/Ind/HAR/CC-156 Non- Structural Protein gene 4(E2 genotype), partial cds (HQ157349). Malik YPS, Chandrashekar KM, Chakravarti S, Sharma K, Vaid N, Sen A, Venketesan G, Bhilegaonkar KN and Pandey AB. 2010. Bovine rotavirus A isolate Bov/Ind/MP/JBP/08/C-51 Non-Structural Protein gene 4 (E2 genotype), partial cds (HQ157350). Chakravarti S, Malik YPS, Vaid N, Sharma K, Basera S, Singh R, Bhilegaonkar KN, Sen A, Venketesan G and Pandey AB. 2010. Bovine rotavirus A isolate Bov/Ind/UKD/PTN/09/15E Non-Structural Protein gene 4 (E2 genotype), complete cds. (HQ171910). Malik YPS, Chakravarti S, Basera S, Singh R, Sharma K, Vaid N, Sen A, Venketesan G, Bhilegaonkar KN and Pandey AB. 2010. Bovine rotavirus A isolate Bov/Ind/UKD/PTN/09/970 major Intermediate capsid protein VP6 (I2 genotype), complete cds. (HQ171911). Malik YPS, Chakravarti S, Vaid N, Sharma K, Chandrashekar KM, Sen A, Venketesan G, Bhilegaonkar KN and Pandey AB. 2010. Bovine rotavirus A isolate Bov/Ind/MP/JBP/08/B100 major intermediate capsid protein VP6 (I2 genotype), complete cds. (HQ171912). Malik YPS, Chakravarti S, Chandrashekar KM, Sharma K, Vaid N, Sen A, Bhilegaonkar KN and Pandey AB. 2010. Bovine rotavirus A isolate Bov/Ind/MP/JBP/08/B-72 major intermediate VP6 capsid protein (I2 genotype), complete cds. (HQ171913). Chakravarti S, Vaid N, Sharma K, Chandrashekar KM, Venketesan G, Sen A, Bhilegaonkar KN, Pandey AB and Malik YPS. 2010. Rotavirus group B Hu/Ind/MP/08/K VP6 intermediate capsid protein gene, partial cds. (HQ199896) Malik YPS, Chakravarti S, Vaid N, Sharma K, Basera S, Singh R, Sen A, Venketesan G, Bhilegaonkar KN and Pandey AB. 2010. Bovine group A rotavirus Bov/India/UKD/PTN/P-970 VP7 major outer capsid protein gene, complete cds. (HQ199897) Chakravarti, S., Chandrashekar, K.M., Sharma, K., Vaid, N., Haq, A., Bhilegaonkar, K.N., Pandey, A.B. and Malik, Y.P.S. 2010. Bovine group A rotavirus Bov/India/MP/2008/B68 non-structural protein 4 (NSP4) gene, Genotype E2, complete cds. GARVB68 739bp RNA 21-OCT-2010 (HQ433435). Chakravarti, S., Chandrashekar, K.M., Sharma, K., Vaid, N., Haq, A., Bhilegaonkar, K.N., Pandey, A.B. and Malik, Y.P.S. 2010. Bovine group A rotavirus Bov/India/HAR/2008/B54 non-structural protein 4 (NSP4) gene, Genotype E1, partial cds. (HQ433436) Malik, Y.P.S., Chandrashekar, K.M., Chakravarti, S., Vaid, N., Sharma, K., Haq, A., Bhilegaonkar, K.M. and Pandey, A.B.2010. Human group B rotavirus JBP/K/2008 non-structural protein 4 (NSP4) gene, complete cds. (HQ433433). Malik, Y.P.S., Chandrashekar, K.M., Chakravarti, S., Vaid, N., Sharma, K., Haq, A., Bhilegaonkar, K.M. and Pandey, A.B.2010. Human group B rotavirus JBP/K/2008 outer capsid structural protein 7 (VP7) gene, complete cds. (HQ433434). Malik, Y.P.S., Chakravarti, S., Vaid, N., Sharma, K., Haq, A., Sen, A., Venketesan, G., Bhilegaonkar, K.N. and Pandey, A.B. 2010. Bovine group A rotavirus India/MP/2008/B85 outer capsid VP4 protein gene, genotype P3, partial cds. (HQ440223). Malik, Y.P.S., Chakravarti, S., Vaid, N., Sharma, K., Haq, A., Sen, A., Venketesan, G., Bhilegaonkar, K.N. and Pandey, A.B. 2010. Bovine group A rotavirus India/MP/2008/B85 outer capsid VP7 protein gene, genotype G3, complete cds. (HQ440224). Malik, Y.P.S., Chakravarti, S., Vaid, N., Sharma, K., Haq, A., Sen, A., Venketesan, G., Bhilegaonkar, K.N. and Pandey, A.B. 2010. Bovine group A rotavirus India/MP/2008/B85 enterotoxin non-structural protein 4 (NSP4) gene, genotype E1, complete cds. (HQ440225). Malik, Y.P.S., Chakravarti, S., Sharma, K., Vaid, N., Haq, A., Bhilegaonkar, K.N., Mohapatra, J.K., Pandey, L.K., De, A. and Pandey, A.B. 2010. Bovine group A rotavirus India/UKD/M1/2010 VP1 gene, genotype R2, complete cds. (HQ440220) Malik, Y.P.S., Chakravarti, S., Sharma, K., Vaid, N., Haq, A., Bhilegaonkar, K.N., Mohapatra, J.K., Pandey, L.K., De, A. and Pandey, A.B. 2010. Bovine group A rotavirus India/UKD/M1/2010 core protein 2 (VP2) gene, genotype C2, complete cds. (HQ440221). Malik, Y.P.S., Chakravarti, S., Sharma, K., Vaid, N., Haq, A., Bhilegaonkar, K.N., Mohapatra, J.K., Pandey, L.K., De, A. and Pandey, A.B. 2010. Bovine group A rotavirus India/UKD/M1/2010 VP3 gene, genotype M2, partial cds. (HQ440222). Malik, Y.P.S., Chakravarti, S., Sharma, K., Vaid, N., Haq, A., Bhilegaonkar, K.N., Mohapatra, J.K., Pandey, L.K., De, A. and Pandey, A.B. 2010. Bovine group A rotavirus India/UKD/M1/2009 outer capsid protein 7(VP7) gene, genotype G3, partial cds. (HM235505). Chakravarti, S., Sharma, K., Vaid, N., Haq, A., Sen, A., Venketesan, G., Bhilegaonkar, K.N., Pandey, A.B. and Malik, Y.P.S. 2010. Bovine group A rotavirus India/UP/Bov2/2008 inner capsid protein 6 (VP6) gene, genotype I2, complete cds. (HQ440218) Chakravarti, S., Sharma, K., Vaid, N., Haq, A., Sen, A., Venketesan, G., Bhilegaonkar, K.N., Pandey, A.B. and Malik, Y.P.S. 2010.Human group A rotavirus India/UP/H2/2008 non-structural protein 4 (NSP4) gene, genotype E2, complete cds. (HQ440219) Haq, A., Malik, Y.P.S., Chakravarti, S., Sharma, K., Vaid, N., Mohapatra, J.K., De, A. and Pandey, A.B. 2011. Bovine rotavirus group A isolate Bov/Ind/UP/10/BE-3 outer capsid VP4 protein (P1 genotype), partial cds. 856 bp. (JF689832) Haq, A., Malik, Y.P.S., Chakravarti, S., Sharma, K., Vaid, N., Mohapatra, J.K., De, A. and Pandey, A.B. 2011. Bovine rotavirus group A isolate Bov/Ind/UP/10/BE-4 outer capsid VP4 protein (P1 genotype), partial cds. 771 bp. (JF689833) Haq, A., Malik, Y.P.S., Chakravarti, S., Sharma, K., Vaid, N., Mohapatra, J.K., De, A. and Pandey, A.B. 2011. Bovine rotavirus group A isolate Bov/Ind/UP/10/Bov-2 outer capsid VP4 protein (P1 genotype), partial cds. 854 bp .(JF689834) Haq, A., Malik, Y.P.S., Chakravarti, S., Sharma, K., Vaid, N., Mohapatra, J.K., De, A. and Pandey, A.B. 2011. Bovine rotavirus group A isolate Bov/Ind/MP/10/MF-10 major outer capsid neutralizing VP7 protein (G3 genotype), complete cds. 1062bp.(JF689835) Haq, A., Malik, Y.P.S., Chakravarti, S., Vaid, N., Sharma, K., Singh, R., De, U.K., Batra, M., Bhilegaonkar, K.N., Prasad, M. and Pandey, A.B. 2011. Bovine group A rotavirus isolate Ind/UP/09/BE3 VP7 protein gene (G6 genotype), partial cds. 791 bp. (JF689841) Haq, A., Malik, Y.P.S., Chakravarti, S., Vaid, N., Sharma, K., Singh, R., De, U.K., Batra, M., Bhilegaonkar, K.N., Prasad, M. and Pandey, A.B. 2011. Bovine group A rotavirus isolateInd/HR/10/BR108 VP7 protein gene (G6 genotype), partial cds. 736 bp.(JF689842) Haq, A., Malik, Y.P.S., Chakravarti, S., Vaid, N., Sharma, K., Singh, R., De, U.K., Batra, M., Bhilegaonkar, K.N., Prasad, M. and Pandey, A.B. 2011. Bovine group A rotavirus isolate Ind/UP/09/Bov1 VP7 protein gene (G3 genotype), partial cds. 1062 bp. (JF689843) Haq, A., Malik, Y.P.S., Chakravarti, S., Vaid, N., Sharma, K., Singh, R., De, U.K., Batra, M., Bhilegaonkar, K.N., Prasad, M. and Pandey, A.B. 2011. Bovine group A rotavirus isolate Ind/UP/09/Bov2 VP7 protein gene (G3 genotype), partial cds. 1062 bp. (JF689844) Haq, A., Malik, Y.P.S., Chakravarti, S., Vaid, N., Sharma, K., Singh, R., De, U.K., Batra, M., Bhilegaonkar, K.N., Prasad, M. and Pandey, A.B. 2011. Bovine group A rotavirus isolate Ind/UP/09/P9 VP7 protein gene (G3 genotype), partial cds. 1062 bp. (JF689845) Haq,A., Malik,Y.P.S., Chakravarti,S., Vaid,N., Sharma,K., Batra,M., Bhilegaonkar, K.N. and Pandey,A.B. 2011. Bovine rotavirus group A isolate Bov/Ind/UKD/10/P9 non-structural protein NSP4 gene (E1 genotype), complete cds. 744 bp. (JF689836) Haq,A., Malik,Y.P.S., Chakravarti,S., Vaid,N., Sharma,K., Batra,M., Bhilegaonkar, K.N. and Pandey,A.B. 2011. Bovine rotavirus group A isolate Bov/Ind/UP/10/Bov1 non-structural protein NSP4 gene (E2 genotype), complete cds. 726 bp. (JF6898370) Haq,A., Malik,Y.P.S., Chakravarti,S., Sharma,K., Vaid,N., Basera,S., Singh,R., Shukla,S., Batra,M. and Pandey,A.B. 2011. Bovine group A rotavirus Bov/Ind/MP/10/MF10 outer capsid VP4 protein gene (P1 genotype), partial cds. 854 bp. (JF689838) Haq,A., Malik,Y.P.S., Chakravarti,S., Sharma,K., Vaid,N., Basera, S., Singh,R., Shukla,S., Batra, M. and Pandey,A.B. 2011. Bovine group A rotavirus Bov/Ind/UKD/09/P9 outer capsid VP4 protein gene (P1 genotype), partial cds. 854 bp. (JF689839) Haq,A., Malik,Y.P.S., Chakravarti,S., Sharma,K., Vaid,N., Basera, S., Singh,R., Shukla,S., Batra,M. and Pandey,A.B. 2011. Bovine group A rotavirus Bov/Ind/UKD/09/15E outer capsid VP4 protein gene (P1 genotype), partial cds. 854 bp. (JF689840) Nucleotide gene bank Submission Toll Like Receptors Malik YPS, Chakravarti S, Sharma K, Vaid N, Singh RK, Mohapatra JK And Pandey AB. Bos grunniens (Yak) Toll Like Receptor 3 (TLR 3) Gene, complete cds.( Jf689830). Kataria RS, Goyal S, Kathiravan P, Mishra BP, Malik YPS, Chakravarti S, Vaid N, Sharma K (2010). Bos frontalis (Gaur) Toll-like receptor 7, exon3, partial cds (HQ327994) Goyal S, Dubey PK, Kathiravan P, Mishra BP, Malik YPS, Chakravarti S, Vaid N, Sharma K and Kataria RS (2010). Bos frontalis Toll-like receptor 4 (TLR4) gene exon 1, exon2 and exon3 (HQ327993). Chakravarti S, Malik YPS, Sharma K, Vaid N, Mohapatra JK, Pandey LK, De A, Singh RK and Pandey AB. 2010. Bos grunniens (Yak) Toll-like receptor 8 (TLR8) gene, complete cds, (HQ392515) Malik YPS, Chakravarti S, Sharma K, Vaid N, Mohapatra JK, Pandey LK, De A, Singh RK and Pandey AB. 2010. Bos frontalis (Mithun) Toll-like receptor 5 (TLR5) gene, complete cds. (HQ392514) Malik YPS, Chakravarti S, Sharma K, Vaid N, Mohapatra JK, Pandey LK, De A, Kataria RS, Raj GD, Singh RK and Pandey AB. 2010. Bos frontalis (Mithun) Toll like receptor 9 (TLR9) gene, complete cds. (HQ199895) Chakravarti S, Malik YPS, Vaid N, Sharma K, Mohapatra JK, Pandey LK, De A, Kataria RS, Raj GD, Singh RK and Pandey AB. 2010. Bos frontalis (Mithun) Toll like receptor 3 (TLR3) gene, complete cds. (HQ199894) Malik YPS, Chakravarti S, Sharma K, Vaid N, Mohapatra JK, Pandey LK, De A, Kataria RS, Raj GD, Pandey AB and Singh RK. 2010. Bos frontalis (Mithun) Toll like receptor 1 (TLR1) gene, complete cds. (HQ199893) Malik YPS, Chakravarti S, Vaid N, Sharma K, Kataria RS, Raj GD Singh RK Pandey AB, Mohapatra JK, Pandey LK and De A. 2010. Bos grunniensis (Yak) Toll like receptor 9 gene, complete cds (HQ199892). Chakravarti S, Malik YPS, Vaid N, Sharma K, Kataria RS, Raj GD Singh RK and Pandey AB, 2010. Bos grunniensis (Yak) Toll like receptor 4 gene, complete cds (HQ199891). Malik YPS, Chakravarti S, Vaid N, Sharma K, Kataria RS, Raj GD Singh RK and Pandey AB, 2010. Bos grunniensis (Yak) Toll like receptor 3 gene, complete cds (HQ199890). Chakravarti S, Malik YPS, Vaid N, Sharma K, Kataria RS, Raj GD and Singh RK, 2010. Bos grunniensis (Yak) Toll like receptor 2 gene, complete cds (HQ199889). Malik YPS, Chakravarti S, Vaid N, Sharma K, Kataria RS, Raj GD and Singh RK, 2010. Bos grunniensis (Yak) Toll like receptor 1 gene, complete cds (HQ199888). Chakravarti, S., Sharma, K., Vaid, N., Rajak, K.K., Biswas, S., Balamurgan, V., Kataria, R.S., Raj, G.D., Mondal, B., Singh, R.K. and Malik, Y.P.S. Bos indicus Toll-like receptor 4 (TLR4) gene exon 3 (P1), partial cds (HM363554) Chakravarti, S., Balamurgan, V., Rajak, K.K., Biswas, S., Vaid, N., Sharma, K., Kataria, R.S., Raj, G.D., Singh, R.K., Mondal, B. and Malik, Y.P.S. Bos indicus Toll like receptor(TLR) 4 gene exon 3 (P2), partial cds (HM363555) Chakravarti, S., Biswas, S., Rajak, K.K., Balamurgan, V., Vaid, N., Sharma, K., Kataria, R.S., Raj, G.D., Mondal, B., Singh, R.K. and Malik, Y.P.S. Bos indicus Toll like receptor (TLR) 4 gene exon 3(P3), partial cds (HM363556). Chakravarti, S., Sharma, K., Vaid, N., Balamurgan, V., Rajak, K.K., Biswas, S., Kataria, R.S., Raj, G.D., Singh, R.K., Mondal, B. and Malik, Y.P.S. Bos indicus Toll like receptor (TLR) 7 gene, CDS(P3), partial cds (HM363557). Chakravarti, S., Balamurgan, V., Vaid, N., Sharma, K., Rajak, K.K., Biswas, S., Kataria, R.S., Raj, G.D., Singh, R.K., Mondal, B. and Malik, Y.P.S. (2010) Bos indicus Toll like receptor (TLR) 7 gene, CDS (P5), partial cds (HM363558). Chakravarti, S., Sharma, K., Vaid, N., Biswas, S., Rajak, K.K., Kataria, R.S., Raj, G.D., Balamurgan, V., Singh, R.K., Mondal, B. and Malik, Y.P.S. Bos indicus Toll like receptor (TLR) 7 gene CDS (P6), partial cds (HM363559) Malik, Y.P.S., Chakravarti, S., Mohapatra, J.K., Sharma, K., Vaid, N., Pandey, L.K. and De, A. (2010). Bos frontalis Toll like receptor 6 gene, partial cds (HM235504). Chakravarti, S., Vaid, N., Sharma, K., Pandey, L.K., Mohapatra, J.K., De, A., Kataria, R.K. and Malik, Y.P.S. (2010). Bos indicus Toll like receptor 7, partial cds (HM235503). Chakravarti,S., Sharma, K., Vaid, N., Biswas, S.K., Kataria, R.S., Raj, G.D., Bhanuprakash, V., Singh, R.K. and Malik, Y.P.S. Bos grunniens (Yak) Toll like receptor 5 (TLR5) from gDNA (GU647093). Sharma, K., Vaid, N., Chakarvarti, S., Biswas, S.K., Kataria, R.S., Raj, G.D., Singh, R.K., Bhanuprakash, V. and Malik, Y.P.S.Bos grunniens (Yak) Toll like receptor 7 (TLR7) gene, exon 3 (CDSP-5), partial cds from Arunachal Pradesh (GU247594). Vaid, N., Sharma, K., Chakarvarti, S., Biswas, S.K., Kataria, R.S., Raj, G.D., Singh, R.K., Bhanuprakash, V. and Malik, Y.P.S Bos grunniens (Yak) Toll like receptor 7 (TLR7) gene, exon 3, partial cds from Arunachal Pradesh (GU247593). Sharma, K., Vaid, N., Chakarvarti, S., Biswas, S.K., Malik, Y.P.S., Mondal, B., Kataria, R.S., Raj, G.D. and Singh, R.K. Bos indicus from Himalayan region Toll like receptor 7 (TLR7) gene, CDSP7, partial cds (GU143097). Mondal, B., Balamurugan, V., Chakravati, S., Rajak, K.K., Biswas, S.K., Sharma, K., Vaid, N., Malik, Y.P.S., Kataria, R.S., Bhanuprakash, V. and Singh, R.K Bos indicus (Himalayan cattle) Toll like receptor 7, mRNA, complete cds (GQ 438260). Mondal, B., Balamurugan, V., Biswas, S. K., Chakravarti, S., Vaid, N., Sharma, K., Malik, Y.P.S., Bhanuprakash, V., Kataria, R.S. and Singh, R.K. Bos grunniens (Yak) Toll like receptor 7, mRNA, complete cds (GQ438259). PERSONEL DETAIL’S NAME –NIRUPMA VED FATHER’S NAME-ASHOK VIKRAM VED DOB-4-09-80 PH-09907193302 Email-Vaidnirupama@gmail.com ADDRESS- BABAI HOUSE GOPAL GANJ ORAI DISTT_JALAUN(U.P.)