Name_Ryanadams___________________________Date______18/02/11________Form____myp4_ ________ Answer your questions in red Save file as FirstName_LastName_BiochemWebquest Example: Kevin_Hall_BiochemWebquest.docx Biochemistry: WebQuest The Chemistry of Life Go to: http://www.biocab.org/Biology.html#anchor_26 Read the chemistry of life section. You may have to scroll up or down a bit to reach it! 1. How many naturally occurring elements are there? __92 elements are naturally occurring____________________ 2. About how many of these elements are found in living matter? _____25_________ 3. Which 4 are present in 97% of the molecules of living matter? 1) _carbon_____, 2) __hydrogen______, 3) ___oxygen_____, 4) _nitrogen_____ 4. What are the main elements in the remaining 3%? __phosphorus sulfur calcium potassium_________________________________ 5. Define an organic molecule. ______a molecule containing carbon_______________________________________________________ 6. Name the 4 main organic molecules? 1) __carbohydrates__________, ___protiens_______ 2) ______lipids_______, 3) ____nucleic acid_________ 4) Organic Molecules lab Go to: http://www.okc.cc.ok.us/biologylabs/ Click on Organic Molecules Click on Carbohydrates Look at the example of a carbohydrate; a monosaccharide (simple sugar). 1. What 3 elements make up this molecule? 1)__carbon_____ 2)_____hydrogen____ 3)__oxeygen________ 2. Count the number of hydrogen and oxygen atoms. What is the ratio of H to O atoms? ______12:5__________ Do the experiment 3.Which color is the positive test for a simple sugar (a carbohydrate) when heated with Benedict’s reagent? _______ orange________________________ 4. Which color is the positive test for starch (a larger carbohydrate)? __________brownish _________________ Click on continue or on organic molecules. Then click on Proteins. 5. Observe the portion of the protein molecule. What are the main elements (R is not an element, it represents a side chain!)? __hydrogen,carbon,nitrogen,oxeygen______________________________________________ Do the experiment. 6. What is a positive test for protein using biuret reagent? ___violet___ ________________________ Click on continue or on organic molecules. Then click on Fats (lipids). 7. Observe the fat (lipid) molecule. What elements make up this molecule? ______mostly hydrogen and carbon but there is oxygen as well____________________ 8. Is the ratio of Hydrogen atoms to Oxygen atoms the same as in the carbohydrate? Yes or No No there is more hydrogen Do the experiment. 9. What color appeared when vegetable oil fat (lipid) was mixed with the Sudan IV? _______redish at the top but more white at the bottom.______________ Click on continue then click on Foods Test the salmon – circle the correct answer 10. Simple Sugar – yes or no, Starch – yes or no, Protein – yes or no, Fat – yes or no Test the milk – circle the correct answer 11. Simple Sugar – yes or no, Starch – yes or no, Protein – yes or no, Fat – yes or no Protein Structure Animation Go to http://www.stolaf.edu/people/giannini/flashanimat/proteins/protein%20structure.swf This animation represents a protein molecule. View the animation and answer the questions 1. A protein is made up of a chain of ______amino acids______ __________. 2. After viewing the animation do you think the shape of a protein molecule is complex? Circle one: Yes or No Explain ____ ____________________________________ 3. Which of the following occurs to form a protein? (circle the correct answer) A) forms a chain of amino acids B) forms a spiral (helix) C) folds on itself (bends) D) More than one chain joins together E) all of these Enzymes Go to http://science.howstuffworks.com/cell2.htm Read the text and answer the following questions 1. What is the purpose of enzymes? ____to carry out chemical reactions rapidly _______________________________________________________ 2. What type of organic molecules (carbohydrate, lipid, protein, nucleic acid) are most enzymes? _proteins (amino acids)_______________________________ 3. What does the shape of an enzyme have to do with how well the enzyme works? ______________________________________________________________ Scroll down to the pink graphic. 4. What is the function of the active site on the enzyme? ____to break the bonds and in that picture it is ro break maltase bonds _________________________________________ 5. In this example the maltase enzyme is speeding up what reaction? – Fill in the answers! The breakup of ________maltase_____ into two ______glucose_________ molecules. The Maltose (a sugar) lands on the ___active__________ site of the enzyme. The enzyme has to have a specific _________ in order for the maltose to connect there. Acids & Bases Go to http://lrs.ed.uiuc.edu/students/erlinger/water/background/ph.html View the first two sections of the page & answer the following questions 1. What is an acid & give 2 examples? __________________________________________________________________ 2. What is a base & give 2 examples? __________________________________________________________________ The pH Scale Go to http://staff.jccc.net/pdecell/chemistry/phscale.html View the page & answer the questions 1. Since atoms have charge and molecules are made of bonded atoms in a specific shape what can a high concentration of positive Hydrogen ions (H+) do to the shape of a molecule? _________________________________________________________________ 2. Can changing their shape change their function (what they do)? Give an example from what you learned about enzymes and their active site. __________________________________________________________________ __________________________________________________________________ 3. What does the pH scale measure? ______________________________________________________________ 4. A low pH corresponds to a high or low hydrogen ion (H+) concentration? Circle the correct answer. 5. A high pH corresponds to a high or low hydrogen (H+) concentration? Circle the correct answer. 6. What substance has a neutral pH of 7? _________________________________ What is the pH of saliva? ___________ Is that slightly acidic or basic? ___________ What is the pH of the acid secreted by your stomach lining? _________________________ What is the pH of soapy water?___________ Is soapy water a base or an acid? _____