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PRIX CENTRE D’ETUDES PRINCESSE
JOSÉPHINE-CHARLOTTE 2011
10 NOVEMBRE 2011
CONTACT :
Christel Buelens
christel.buelens@frs-fnrs.be
02/504.92.96
F.R.S. – FNRS
Fonds de la Recherche Scientifique-FNRS
REMISE DU « PRIX CENTRE D’ETUDES PRINCESSE JOSÉPHINE-CHARLOTTE 2011 »
LE JEUDI 10 NOVEMBRE 2011 À 11H30
En vue de soutenir la recherche scientifique dans le domaine de la lutte contre les infections
virales, le Centre d’Etudes Princesse Joséphine-Charlotte a chargé le Fonds de la Recherche
Scientifique – FNRS d’attribuer, tous les deux ans, un Prix de 12.500 €.
Ce 10 novembre 2011, le F.R.S. – FNRS procède, en présence de représentants du monde
universitaire et du Centre d’Etudes Princesse Joséphine-Charlotte, à la remise du douzième
Prix biennal.
Le Prix 2011 a été attribué à :
Herman FAVOREEL,
Bio-Ir. Cel- en Genbiotechnologie (U.Gent),
Dr. Diergeneeskundige wetenschappen (U.Gent),
Hoofddocent (U.Gent),
pour son travail :
Alphaherpesvirus cell-to-cell spread through manipulation of the actin cytoskeleton by the viral
US3 protein kinase
Herman FAVOREEL – Ghent University (UGent)
Viruses are obligate intracellular parasites, only able to replicate inside a host cell. To be able
to reach and infect new host cells, viruses must exit a previously infected cell and travel
through the extracellular milieu to a new host cell. However, during passage in the
extracellular space, viral pathogens face hostile components of the immune system such as
neutralizing antibodies. Alternatively, some viruses have developed strategies to spread
directly from one cell to another (direct cell-to-cell spread), thereby avoiding the inhospitable
extracellular space.
We have discovered a new mode of such direct cell-to-cell spread by herpesviruses, which
constitute some of the most successfull and important viral pathogens worldwide. We have
found that the US3 protein kinase of herpesviruses forces the infected cell to remodel its actin
cytoskeleton, resulting in the formation of long cell projections. These cell projections contact
distant uninfected cells and firmly attach to these uninfected cells. Virus particles use these
cell projections as tunnels to efficiently reach and infect the contacted distant cells. Inside
these ‘tunnels’, the viruses are shielded from virus-neutralizing antibodies in the extracellular
milieu. We found that the US3 protein kinase triggers the actin changes and cell projections in
infected cells by activating cellular p21-activating kinases, which are key components of the
cellular signaling pathways that regulate the actin cytoskeleton. These findings shed new light
on the complex and fascinating interaction of herpesviruses with host cells and the immune
system, and identify novel potential targets for the development of antiviral strategies.
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Monsieur Herman FAVOREEL
Hoofddocent à l’Universiteit Gent
“Alphaherpesvirus cell-to-cell spread through manipulation of the actin
cytoskeleton by the viral US3 protein kinase”
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“Alphaherpesvirus cell-to-cell spread through manipulation of the actin cytoskeleton by the
viral US3 protein kinase”
Herman FAVOREEL – Ghent University (UGent)
Viruses are obligate intracellular parasites. In other words, upon infection of a host, they can
only replicate inside a cell of this host since they need the enzymatic machinery and building
blocks of a host cell for their own replication. Once replication is completed, often with the
production of hundreds or thousands of new virus particles, viruses will leave the infected cell
and infect a new cell to start another replication cycle.
In order to reach a new host cell, viruses must travel some time through the extracellular
milieu. During this passage in the extracellular space, the virus is vulnerable, and may be
intercepted and/or destroyed by components of the antiviral immune system of the host,
including virus-neutralizing antibodies.
Hence, it may come as no surprize that many of the most successfull viruses have developed
alternative modes of transmission from an infected to an uninfected cell. These consist of
direct cell-to-cell spread, thereby limiting or avoiding passage through the inhospitable
extracellular space.
Herpesviruses indisputably belong to the most successfull viral pathogens worldwide. They
most likely have been around for millions of years, co-evolving with their hosts along the way.
This has allowed each of the herpesviruses to establish lifelong dormant infections in their host.
Specific stimuli may reactivate these dormant herpesviruses, leading to lifelong periods of
recurrent symptoms and transmission to new hosts. A typical herpesvirus in humans is the
herpes simplex virus, causing recurrent oral and genital lesions. Recurrent reactivation and
disease caused by herpesviruses showcases the ability of these pathogens to circumvent
efficient elimination by the immune system of the host, including virus-neutralizing antibodies.
Identifying the strategies employed by herpesviruses to avoid elimination by the immune
system is pivotal, not only in gaining fundamental insights in the biology of these important
and highly successfull viruses, but also to design new antiviral strategies and vaccines.
We have discovered an entirely novel mode of herpesvirus direct cell-to-cell spread from an
infected to an uninfected cell. We have found that a viral protein, the US3 protein kinase,
forces the infected host cell to reorganize its actin cytoskeleton. The actin cytoskeleton
constitutes a fundamental aspect of the ‘skeleton’ of a cell, and changes in the actin
cytoskeleton may therefore drive substantial changes in the shape and behaviour of a cell.
We have found that these virus-induced changes in the actin cytoskeleton result in the
formation of very long cell projections. These cell projections make contact with other, distant
cells, and firmly attach to these cells. Video microscopy of fluorescently labeled viruses
showed that the viruses use these cell projections as a kind of tunnel. They rapidly migrate
inside the projections, followed by transmission into the contacted, previously uninfected host
cells. Addition of virus-neutralizing antibodies could not prevent this mode of spread,
indicating that using these cellular ‘tunnels’, the virus does not expose itself to the virusneutralizing antibodies in the extracellular milieu. We have demonstrated that the US3 protein
of herpesviruses induces these actin and cell shape changes by phosphorylating and thereby
activating p21-activated kinases, which are central components in the cellular signaling
pathways that regulate the actin cytoskeleton.
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These findings create new insights in the complex and fascinating interactions between
herpesviruses and their host cells, and open up new avenues in the design of antiviral
strategies, by targeting the viral proteins responsible for triggering these events (i.e. the US3
protein kinase) and/or the cellular proteins that drive the resulting actin and cell shape
changes (i.e. p21-activated kinases).
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CURRICULUM VITAE
EDUCATION & CAREER
Bio-ingenieur in de cel- en genbiotechnologie
Ghent University, Faculty of Applied Biological Sciences
1990 – great distinction
Doctoraat in de diergeneeskundige wetenschappen
Ghent University, Faculty of Veterinary Medicine
Promoter: H. Nauwynck
1999 – greatest distinction
Postdoctoral fellow
Princeton University, Department of Molecular Biology
Fullbright and B.A.E.F. Postdoctoral Fellow
PI: L. Enquist
2000 – 2001
Postdoctoral researcher
Ghent University, Faculty of Veterinary Medicine
PI: M. Pensaert/H. Nauwynck
2001 – 2004
Assistant professor (docent – BOF-ZAP)
Ghent University, Faculty of Veterinary Medicine
2004 – 2010
Associate professor (hoofddocent – BOF-ZAP)
Ghent University, Faculty of Veterinary Medicine
2010 – present
HONORS & AWARDS
2000:
2000:
2007:
Fulbright postdoctoral fellowship
Belgian American Educational Foundation postdoctoral fellowship
Prijs van de Koninklijke Academie voor Geneeskunde – afdeling Diergeneeskunde
2004-2007
PROFESSIONAL EXPERIENCE
2011 – present: Editor of PLoS ONE
2005 – present: Board Member of the Belgian Society for Microbiology
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2005 – present: Chair ‘Veterinary Satellite Meeting’ of the annual International
Herpesvirus Workshop (IHW)
2011: Member of the Scientific Committee of the IHW, Gdansk,
Poland, July 22-28
2005: Chair ‘2nd ESVV Veterinary Herpesvirus Symposium’, March 2-4
2005 – present: coordinator ‘Research’ option Master in Veterinary Medicine
SELECTED PUBLICATIONS
Favoreel, H.W., Van Minnebruggen, G., Adriaensen, D., & Nauwynck, H.J. (2005). Cytoskeletal
rearrangements and cell extensions induced by the US3 kinase of an alphaherpesvirus are
associated with enhanced spread. PNAS (Proceeding of the National Academy of
Sciences of the USA), 102, 8990-8995.
Favoreel, H.W., Enquist, L.W., & Feierbach, B. (2007). Review: Actin and Rho GTPases in
herpesvirus biology. Trends in Microbiology, 15, 426-433.
De Regge, N., Nauwynck, H.J., Geenen, K., Krummenacher, C., Cohen, G., Eisenberg, R.,
Mettenleiter, T.C., & Favoreel, H.W. (2006). Alpha-herpesvirus gD interaction with sensory
neurons triggers formation of varicosities that serve as virus exit sites. Journal of Cell Biology,
174, 267-275.
Van den Broeke, C., Radu, M., Deruelle, M., Nauwynck, H., Hofmann, C., Jaffer, Z., Chernoff,
J., & Favoreel, H.W. (2009). Alphaherpesvirus US3-mediated reorganization of the actin
cytoskeleton is mediated by group A p21-activated kinases. PNAS (Proceeding of the
National Academy of Sciences of the USA), 106, 8707-8712.
Van den Broeke, C., Radu, M., Chernoff, J., & Favoreel, H.W. (2010). An emerging role for p21activated kinases (Paks) in viral infections. Trends in Cell Biology, 20, 160-169.
PEER-REVIEWED PUBLICATIONS
Favoreel, H.W., Nauwynck, H.J., Van Oostveldt, P., Mettenleiter, T.C. & Pensaert, M.B. (1997).
Antibody-induced and cytoskeleton-mediated redistribution and shedding of viral
glycoproteins, expressed on pseudorabies virus infected cells. Journal of Virology 71, 82548261.
Duan, X., Nauwynck, H.J., Favoreel, H.W. & Pensaert, M.B. (1998). Identification of a putative
receptor for porcine reproductive and respiratory syndrome virus on porcine alveolar
macrophages. Journal of Virology 72, 4520-4523.
Favoreel, H.W., Nauwynck, H.J., Halewyck, H.M., Van Oostveldt, P., Mettenleiter, T.C. &
Pensaert, M.B. (1999). Antibody-induced endocytosis of viral glycoproteins and major
histocompatibility complex class I on pseudorabies virus-infected monocytes. Journal of
General Virology 80, 1283-1291.
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Favoreel, H.W., Nauwynck, H.J. & Pensaert, M.B. (1999). Role of the cytoplasmic tail of gE
during antibody-induced redistribution of viral glycoproteins, expressed on pseudorabies
virus-infected cells. Virology 259, 141-147.
Nauwynck, H.J., Duan, X., Favoreel, H.W., Van Oostveldt, P. & Pensaert, M.B. (1999). Entry of
porcine reproductive and respiratory syndrome virus into porcine alveolar macrophages
via receptor-mediated endocytosis. Journal of General Virology 80, 297-305.
Favoreel, H.W., Nauwynck, H.J., Van Oostveldt, P. & Pensaert, M.B. (2000). Role of anti-gB and
-gD antibodies in antibody-induced endocytosis of viral and cellular cell surface
glycoproteins on pseudorabies virus-infected monocytes. Virology 267,151-158.
Favoreel, H.W., Nauwynck, H.J. & Pensaert, M.B. (2000). Immunological hiding of herpesvirusinfected cells. Archives of Virology 145, 1269-1290.
Nauwynck, H., Favoreel, H., & Pensaert, M. (2000). Functions of viral glycoproteins in the
pathogenesis of Aujeszky’s disease virus in pigs. Veterinary Research 31, 116-117.
Favoreel, H.W., Nauwynck, H.J., Van Oostveldt, P., & Pensaert, M.B. (2000). Antibody-induced
endocytosis of viral glycoproteins, expressed on pseudorabies virus-infected monocytes
protects these cells from complement-mediated lysis. Veterinary Research 31, 136-137.
Van de Walle, G.R., Favoreel, H.W., Nauwynck, H.J., Van Oostveldt, P. & Pensaert, M.B. (2001).
Involvement of cellular cytoskeleton proteins in antibody-induced internalization of viral
glycoproteins in pseudorabies virus-infected monocytes. Virology 288, 129-138.
Van Minnebruggen, G., Van de Walle, G.R., Favoreel, H.W., Nauwynck, H.J. & Pensaert, M.B.
(2002). Temporary disturbance of actin stress fibers in swine kidney cells during a
pseudorabies virus infection. Veterinary Microbiology 86, 89-94.
Van de Walle, G.R., Favoreel, H.W., Nauwynck, H.J., Van Oostveldt, P. & Pensaert, M.B. (2002).
Antibody-induced internalization of viral glycoproteins in pseudorabies virus-infected
monocytes and role of the cytoskeleton: a confocal study. Veterinary Microbiology 86, 5157.
Favoreel, H.W., Van Minnebruggen, G., Nauwynck, H.J., Enquist, L.W. & Pensaert, M.B. (2002).
A tyrosine-based motif in the cytoplasmic tail of pseudorabies virus glycoprotein B is
important both for antibody-induced internalization of viral glycoproteins and efficient
cell-to-cell spread. Journal of Virology, 76, 6845-6851.
Favoreel, H.W., Van de Walle, G.R., Nauwynck, H.J. & Pensaert, M.B. (2003). Virus complement
evasion strategies. Journal of General Virology, 84, 1-15.
Van de Walle, G.R., Favoreel, H.W., Nauwynck, H.J., Mettenleiter, T.C. & Pensaert, M.B. (2003).
Transmission of pseudorabies virus from the immuno-masked monocyte to endothelial cells
in vitro. Journal of General Virology, 84, 629-637.
Van de Walle, G.R., Favoreel, H.W., Nauwynck, H.J. & Pensaert, M.B. (2003). Antibodyinduced internalization of viral glycoproteins and gE-gI Fc receptor activity protect
pseudorabies virus-infected monocytes from complement-mediated lysis. Journal of
General Virology, 84, 939-947.
Van Minnebruggen, G., Favoreel, H.W., Nauwynck, H.J., Jacobs, L.. & Pensaert, M. B. (2003).
Pseudorabies virus US3 protein kinase mediates actin stress fiber breakdown. Journal of
Virology, 77, 9074-9080.
Vanderheijden, N. Delputte, P., Favoreel, H.W., Vandekerckhove, J., Van Damme, J., Van
Woensel, P.A. & Nauwynck, H.J. (2003). Sialoadhesin can serve as an endocytic receptor
mediating porcine arterivirus entry in alveolar macrophages. Journal of Virology, 77, 82078215.
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Favoreel, H.W., Van de Walle, G.R., Nauwynck, H.J. & Pensaert, M.B. (2003). Pseudorabies
virus-specific antibodies suppress intracellular viral protein expression in pseudorabies virusinfected monocytes. Journal of General Virology, 84, 2969-2973.
Favoreel, H.W., Mettenleiter, T.C. & Nauwynck, H.J. (2004). Co-patching and lipid raft
association of different viral glycoproteins, expressed on the surface of pseudorabies virusinfected cells. Journal of Virology, 78, 5279-5287. + cover
Pensaert, M., Labarque, G., Favoreel, H., & Nauwynck H. (2004). Aujeszky's disease
vaccination and differentiation of vaccinated from infected pigs. Developments in
Biologicals, 119, 243-254.
Van Minnebruggen, G., Favoreel, H.W., & Nauwynck, H.J. (2004). Internalization of
pseudorabies virus glycoprotein B is mediated by an interaction between the YQRL in its
cytoplasmic tail and the clathrin-associated AP-2 adaptor complex. Journal of Virology,
78, 8852-8859.
Geenen, K., Favoreel, H.W., Olsen, L.-A., Enquist, L.W., & Nauwynck, H.J. (2005). The
pseudorabies virus US3 protein kinase possesses anti-apoptotic activity that protects cells
from apoptosis during infection and after treatment with sorbitol or staurosporine. Virology,
331, 144-150.
Geenen, K., Favoreel, H.W., & Nauwynck, H.J. (2005). Higher resistance of porcine trigeminal
ganglion neurons towards pseudorabies virus-induced cell death compared to other
porcine cell types in vitro. Journal of General Virology, 86, 1251-1260.
Ficinska, J., Nauwynck, H.J., Van Minnebruggen, G., Bienkowska-Szewczyk, K., & Favoreel,
H.W. (2005). Pseudorabies virus glycoprotein gD contains a functional endocytosis motif
that acts in concert with an endocytosis motif in gB to drive internalization of antibodyantigen complexes from the surface of infected monocytes. Journal of Virology, 79, 72487254.
Favoreel, H.W., Van Minnebruggen, G., Adriaensen, D., & Nauwynck, H.J. (2005). Cytoskeletal
rearrangements and cell extensions induced by the US3 kinase of an alphaherpesvirus are
associated with enhanced spread. PNAS (Proceeding of the National Academy of
Sciences of the USA), 102, 8990-8995.
Ceelen, L.M., Haesebrouck, F., Favoreel, H., Ducatelle, R., & Decostere, A., (2006). The
cytolethal distending toxin among Helicobacter pullorum strains from human and poultry
origin. Veterinary Microbiology, 113, 45-53.
van der Meulen, K.M., Favoreel, H.W., Pensaert, M.B. & Nauwynck, H.J. (2006). Immune
escape of equine herpesvirus 1 and other herpesviruses of veterinary importance.
Veterinary Immunology and Immunopathology, 111, 31-40.
Geenen, K., Favoreel, H.W., & Nauwynck, H.J. (2006). Cell-type specific resistance of
trigeminal ganglion neurons towards apoptotic stimuli. Veterinary Microbiology, 113, 223229.
Favoreel, H.W., Van Minnebruggen, G., Van de Walle, G., Ficinska, J., & Nauwynck, H.J.
(2006). Herpesvirus interference with virus-specific antibodies: bridging antibodies,
internalizing antibodies, and hiding from antibodies. Veterinary Microbiology, 113, 257-263.
De Regge, N., Favoreel, H.W., Geenen, K., & Nauwynck, H.J. (2006). A homologous in vitro
model to study interactions between alphaherpesviruses and trigeminal ganglion neurons.
Veterinary Microbiology, 113, 251-255.
Favoreel, H.W. (2006). The why’s of Y-based motifs in alphaherpesvirus envelope proteins. Virus
Research, 117, 202-208.
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De Regge, N., Nauwynck, H.J., Geenen, K., Krummenacher, C., Cohen, G., Eisenberg, R.,
Mettenleiter, T.C., & Favoreel, H.W. (2006). Alpha-herpesvirus gD interaction with sensory
neurons triggers formation of varicosities that serve as virus exit sites. Journal of Cell Biology,
174, 267-275.
Glorieux, S., Van den Broeck, W., van der Meulen, K., Van Reeth, K., Favoreel, H.W., &
Nauwynck, H.J. (2007). In vitro culture of porcine respiratory nasal mucosa explants for
studying the interaction of porcine viruses with the respiratory tract. Journal of Virological
Methods, 142, 105-112.
Mateusen, B., Van Soom, A., Maes, D.G.D., Favoreel, H., & Nauwynck, H.J. (2007). Receptordetermined susceptibility of preimplantation embryos to pseudorabies virus and porcine
reproductive and respiratory syndrome virus. Biology of Reproduction, 76, 415-423.
Nauwynck, H., Glorieux, S., Favoreel, H., & Pensaert, M. (2007). Cell biological and molecular
characteristics of pseudorabies virus infections in cell cultures and in pigs with emphasis on
the respiratory tract. Veterinary Research, 38, 229-241.
Geenen, K., Nauwynck, H.J., De Regge, N., Braeckmans, K., & Favoreel, H.W. (2007). Brn-3a
suppresses pseudorabies virus-induced cell death in sensory neurons. Journal of General
Virology, 88, 743-747. + cover
Desplanques, A.S., Nauwynck, H.J., Tilleman, K., Deforce D., & Favoreel, H.W. (2007). Tyrosine
phosphorylation and lipid raft association of pseudorabies virus glycoprotein E during
antibody-mediated capping. Virology, 362, 60-66. + cover
Van Deun, K., Haesebrouck, F., Heyndrickx, M., Favoreel, H.W., Dewulf, J., Ceelen, L., Dumez,
L., Messens, W., Van Immerseel, F., Ducatelle, R., & Pasmans, F. (2007). Virulence properties
of Campylobacter jejuni isolates from poultry and human origin. Journal of Medical
Microbiology, 56, 1284-1289.
Deruelle, M., Geenen, K., Nauwynck, H.J., & Favoreel, H.W. (2007). A point mutation in the
putative ATP-binding site in the pseudorabies virus US3 protein kinase prevents Bad
phoshorylation and cell survival following apoptosis induction Virus Research, 128, 65-70.
Favoreel, H.W., Enquist, L.W., & Feierbach, B. (2007). Review: Actin and Rho GTPases in
herpesvirus biology. Trends in Microbiology, 15, 426-433.
Boyen, F., Pasmans, F., Van Immerseel, F., Morgan, E., Botteldoorn, N., Heyndrickx, M., Volf, J.,
Favoreel, H., Hernalsteens, J.-P., Ducatelle, R., & Haesebrouck, F. (2008). A limited role for
SsrA/B in persistent Salmonella Typhimurium infections in pigs. Veterinary Microbiology, 128,
364-373.
Desplanques, A.S., Nauwynck, H.J., Vercauteren, D., & Favoreel, H.W. (2008). Plasma
membrane cholesterol is required for efficient pseudorabies virus entry. Virology, 376, 339345.
Glorieux, S., Favoreel, H.W., Van den Broeke, W., & Nauwynck, H.J. (2008). Different replication
characteristics of historical pseudorabies virus strains in porcine respiratory nasal mucosa
explants. Veterinary Microbioogy, 136, 341-346.
Ceelen, L.M., Haesebrouck, F., D’Herde, K., Krysko, D.V., Favoreel, H., Vandenabeele, P.,
Ducatelle, R., & Decostere, A. (2008). Mitotic catastrophe as a prestage to necrosis in
mouse liver cells treated with Helicobacter pullorum sonicates. Journal of Morphology, 270,
921-928.
Goossens, K., Van Soom, A., Van Zeveren, A., Favoreel, H., & Peelman, L.J. (2009).
Quantification of Fibronectin 1 (FN1) splice variants, including two novel ones, and analysis
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of integrins as candidate FN1 receptors in bovine preimplantation embryos. BMC
Developmental Biology, Jan 6, 9, 1.
Van den Broeke, C., Deruelle, M., Nauwynck, H.J., Coller, K., Smith, G.A., & Favoreel, H.W.
(2009). The kinase activity of pseudorabies virus US3 is required for its actin modulating
activity. Virology, 385, 155-160. + cover
Van den Broeke, C., Radu, M., Deruelle, M., Nauwynck, H., Hofmann, C., Jaffer, Z., Chernoff,
J., & Favoreel, H.W. (2009). Alphaherpesvirus US3-mediated reorganization of the actin
cytoskeleton is mediated by group A p21-activated kinases. PNAS (Proceeding of the
National Academy of Sciences of the USA), 106, 8707-8712.
Thys, M., Nauwynck, H., Maes, D., Hoogewijs, M., Vercauteren, D., Favoreel, H., & Van Soom,
A. (2009). Expression and putative function of fibronectin and its receptor (integrin alpha5
beta1) in male and female gametes during bovine fertilization in vitro. Reproduction, 138,
471-482.
Van de Walle, G.R., Cox, E., Nauwynck, H.J., & Favoreel, H.W. (2009). The role of dendritic cells
in alphaherpesvirus infections: archetypes and paradigms. Reviews in Medical Virology, 19,
338-358.
Deruelle, M., Van den Broeke, C., Nauwynck, H., Mettenleiter, T.C., & Favoreel, H.W. (2009).
Pseudorabies virus US3- and UL49.5-dependent and-independent mechanisms of MHC I
cell surface expression in different cell types.Virology, 395, 172-181.
Deruelle, M., De Corte, N., Englebienne, J., Nauwynck, H., & Favoreel, H.W. (2010).
Pseudorabies virus US3-mediated inhibition of apoptosis does not affect infectious virus
production. Journal of General Virology, 91, 1127-1132.
Van den Broeke, C., Radu, M., Chernoff, J., & Favoreel, H.W. (2010). An emerging role for p21activated kinases (Paks) in viral infections. Trends in Cell Biology, invited review, 20, 160-169.
Favoreel, H.W., Van den Broeke, C., Desplanques, A., Deruelle, M., Van Minnebruggen, G.,
Nauwynck, H., Glorieux, S., Van Opdenbosch, N., & De Regge, N. (2010). Alphaherpesvirus
use and misuse of cellular actin and cholesterol. Veterinary Microbiology, 143, 2-7.
Rasschaert, K., Devriendt, B., Favoreel, H., Goddeeris, B., & Cox, E. (2010). Clathrin-mediated
endocytosis and transcytosis of enterotoxigenic Escherichia coli F4 fimbriae in porcine
intestinal epithelial cells. Veterinary Immunology and Immunopathology, 137, 243-250.
Desplanques, A., Pontes, M., De Corte, N., Verheyen, N., Nauwynck, H., Vercauteren, D., &
Favoreel, H.W. (2010). Cholesterol depletion affects infectivity and stability of pseudorabies
virus. Virus Research, 152, 180-183.
Baré, J., Sabbe, K., Huws, S., Vercauteren, D., Braeckmans, K., van Gremberghe, I., Favoreel,
H., & Houf, K. (2010). Influence of temperature, oxygen and bacterial strain identity on the
association of Campylobacter jejuni with Acanthamoeba castellani. FEMS Microbiology
Ecology, 74, 371-381.
De Regge, N., Van Opdenbosch, N., Nauwynck, H., Efstathiou, S., & Favoreel, H.W. (2010).
Interferon alpha induces establishment of alphaherpesvirus latency in sensory neurons in
vitro. PloS ONE, 5, e13076.
Vandaele, L., Wesselingh, W., De Clercq, K., De Leeuw, I., Favoreel, H., Van Soom, A., &
Nauwynck, H. (2011). Susceptibility of in vitro produced hatched bovine blastocysts to
infection with bluetongue virus serotype 8. Veterinary Research, 42, 14.
Delputte, P.L., Van Gorp, H., Favoreel, H.W., Hoebeke, I., Delrue, I., Dewerchin, H., Verdonck,
F., Verhasselt, B., Cox, E., & Nauwynck, H.J. (2011). Porcine sialoadhesin (CD169/Siglec-1) is
11
an endocytic receptor that allows targeted delivery of toxins and antigens to
macrophages. PLoS ONE, in press.
Glorieux, S., Favoreel, H.W., Steukers, L., & Nauwynck, H.J. (2011). A trypsin-like serine protease
is involved in pseudorabies virus invasion through the basement membrane barrier of
porcine nasal respiratory mucosa. Veterinary Research, in press.
Deruelle, M., & Favoreel, H.W. (2011). Keep it in the subfamily: the conserved alphaherpesvirus
US3 kinase. Journal of General Virology, 92, 18-30.
Van den Broeke, C., Radu, M., Nauwynck, H.J., Chernoff, J., & Favoreel, H.W. (2011). Role of
group A p21-activated kinases in the anti-apoptotic activity of the pseudorabies virus US3
protein kinase. Virus Research, 155, 376-380.
Jacob, T., Van den Broeke, C., & Favoreel, H.W. (2011). Viral serine/threonine protein kinases.
Journal of Virology, 85, 1158-1173.
Vandaele, L., Wesselingh, W., De Clercq, K., De Leeuw, I., Favoreel, H., Van Soom, A., &
Nauwynck, H. (2011). Susceptibility of in vitro produced hatched bovine blastocysts to
infection with bluetongue virus serotype 8. Veterinary Research, 42, 14.
Delputte, P.L., Van Gorp, H., Favoreel, H.W., Hoebeke, I., Delrue, I., Dewerchin, H., Verdonck,
F., Verhasselt, B., Cox, E., & Nauwynck, H.J. (2011). Porcine sialoadhesin (CD169/Siglec-1) is
an endocytic receptor that allows targeted delivery of toxins and antigens to
macrophages. PLoS ONE, in press.
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MEMBRES DU JURY DU « PRIX CENTRE D’ETUDES PRINCESSE JOSÉPHINE-CHARLOTTE 2011 »
De h. GOOSSENS Herman
Hoogleraar aan de Universiteit Antwerpen
Medische Microbiologie
Mevr. MATHIEU Chantal
Buitengewoon hoogleraar aan de Katholieke
Universiteit Leuven
Laboratory for Experimental Medicine and
Endocrinology (LEGENDO)
M. MICHIELS Thomas
Professeur à l’Université Catholique de Louvain
Pathologie cellulaire et moléculaire
De h. MUYLDERMANS Serge
Hoofddocent aan de Vrije Universiteit Brussel
Cellulaire en Moleculaire Immunologie
De h. NAUWYNCK Hans
Hoogleraar aan de Universiteit Gent
Virologie, Parasitologie, Immunologie
De h. PELEMAN Renaat
Deeltijds hoofddocent aan de Universiteit Gent
Inwendige Ziekten
M. RENAULD Jean-Christophe
Professeur à l'Université Catholique de Louvain
Médecine expérimentale
M. THIRY Etienne
Professeur à l’Université de Liège
Virologie
M. van den BERG Thierry
Président du Jury
Directeur opérationnel du Centre d'Etudes et de
Recherches vétérinaires et agrochimiques (CERVA)
Virologie et Immunologie aviaires
Mme VAN LINT Carine
Directeur de recherches du F.R.S.-FNRS
Université Libre de Bruxelles
Chimie biologique, Virologie moléculaire
* * *
M. CONTENT Jean
Directeur honoraire de l'Institut Pasteur de Bruxelles
Administrateur du Centre d’Etudes
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