Lecture 27 Special Topics - Drug Discovery: Synthetic Research: Natural Product Isolation Molecular Modification Anesthetics This Week in Lab: Spec Unknown (Ch 12) Final Report Next Week in Lab: Synthetic #2 Final Report; Lab Clean-up & Check-out! Bring in your graded PreLabs! Synthesis Research H O Nakadomarin A N H N Why an attractive target? • Unusual ring system • Anticancer, antibacterial and antifungal properties Nakadomarin A H O N H N Isolated in 1997 by Kobayashi and co-workers from an Okinawan marine sponge: 1. Extracted sponge (1 kg) with methanol 2. Extracted methanol layer with ethyl acetate 3. Purified 51.1 g by column chromatography 3X: (a) chloroform/methanol mobile phase (b) cyclohexane/acetone/diethyl amine mobile phase (c) chloroform/methanol mobile phase Only 6.0 mg of Nakadomarin A was isolated! Other related compounds were also isolated from this procedure. Nakadomarin A H O Structure Determination: N H N 1. Molecular formula was established as C26H36N2O by mass spectrometry 2. 1H and 13C NMR data 3. Advanced two-dimensional NMR studies to confirm structure and determine stereochemistry of stereocenters Nakadomarin A Only 6.0 mg of Nakadomarin A was isolated from 1 kg of sponge! Therefore, synthesis is needed to produce mass quantities. Retrosynthetic Analysis: H O N H O O N 1 HN P O SO2Ar N N H P' N 2 3 P SnBu3 N N P' 4 O2N O N + H N SiMe3 5 6 P' Taxol® AcO O Ph O NH OH O Ph O H O OH OH Treatment of: • Lung, ovarian, and breast cancers • Prevention of restenosis Marketed by Bristol-Myers Squibb in 1993 2000 annual sales - $1.6 billion OAc OCOPh Taxol® AcO O Ph NH O OH O Ph O H O OH OH OAc OCOPh • Discovered in 1967 • Isolated from the bark of the Pacific yew tree • Need six 100-year old trees to treat one patient! Thus, a need for synthesis! • Can be made from 10-deacetylbaccatin (an extract from various yew-related species) in several steps (semisynthesis) OR • Can be made from plant cell fermentation technology: (1) propagation of a specific taxus cell line (2) taxol is isolated via extraction, purified by chromatography and crystallization A Taxol Derivative 1H NMR Spectrum: QuickTime™ and a TIFF (LZW) decompressor are needed to see this picture. Molecular Modification Process: 1. A compound is isolated from nature 2. Its basic structure serves as a prototype (a.k.a. a lead compound) in search for other biologically active compounds 3. Analogs of the lead compound are synthesized and tested for biological activity Classic example: cocaine Cocaine Amine (tertiary) methyl ester H3C N 7-membered ring CO2CH3 ester O O • Isolated from the leaves of Erythroxylon coca (South American Andes) • Effective local anesthetic • Produces disturbing effects on the central nervous system (CNS); initial euphoria to severe depression • Goal: identify the portion of cocaine that serves as the local anesthetic; make an anesthetic with no CNS effects Molecular Modification of Cocaine Identify the beneficial portion: Degrade the cocaine molecule step by step by: 1. Removing the CO2CH3 group 2. Cleaving the 7-membered ring Result: An improved lead compound: 1. Aromatic ester 2. Terminal tertiary amine H3C N CO2CH3 O O CH3 N O H3C O Molecular Modification of Cocaine Hundreds of esters synthesized: • Putting groups on aromatic ring • Changing the alkyl groups bonded to nitrogen • Changing the length of the alkyl chain CH3 N O H3C O New lead compound Successful Anesthetics NH2 H3CH2C O N CH2CH3 Procaine (Novocainīĸ) O • Contains an ester that is easily hydrolyzed, thus a short lifetime. • First synthesized in 1905 • Used primarily in dentistry • Primary ingredient in the preparation of Gerovital H3 (remedy aging effects), but this claim was studied and discredited in the 1960’s Successful Anesthetics H3C H3CH2C H3CH2C N Lidocaine (Xylocaine) HN C O H3C • Contains amide that is not so easily hydrolyzed - faster acting and longer lasting than Novocain • Popular local anesthetic used in dentistry or topically • Developed in 1943