Instructor: Colleen Malone Office Hours: After school by appointment only. E-mail Address: cmalone@conejo.k12.ca.us (email is the best way to contact me) Telephone: 498-3676 ext. 1307 Website: http://newburyparkhighschool.net/malone/ My Website Who is Mrs. Malone? Careers: Texas A&M Health Science Center UCLA Amgen Newbury Park High School Who is Mrs. Malone? Classes: Biology-CP Biology-H Marine Science-CP Biotechnology-CP Warm Up (to review or introduce new material) Introduction/Demonstration/Current Events Lecture and Classroom Discussion Laboratory Work Audio Visual Connection and Summary •Introduction to biotechnology •Biology review -(biochemistry, cells, DNA structure/replication; protein structure/synthesis) •Chemistry review -molarity, making solutions, concentrations Techniques: Micropipeting, Gel electrophoresis (agarose gels), Growing bacteria (LB agar plates/ LB broth) Gel Electrophoresis Bacterial Cell Cultures •Amgen-Bruce Wallace Program OR •Coastal Marine Biolabs (DNA Barcoding Project) Techniques: DNA extraction, Bacterial transformation, Polymerase Chain Reaction, DNA sequencing DNA Extraction/Purification Bacterial Transformation Special genes from fireflies, for instance, have been inserted into cells using the crown gall bacterium to produce glowing plants PCR DNA Sequencing Grading will be based on the following percentages & using category weights. Classwork/Homework LATE Passes? 10% Tests/Quizzes 55% Labs / Projects (includes lab notebook) 25% Final Exam/Project 10% Course grades will be determined by percent weights in several categories. The final grade will be based on the following percentages: 93 - 100 90-92 83 - 89 80-82 73-79 A AB BC 70-72 63-69 60-62 0-59 CD DF Homework will be assigned on a regular basis; Monday through Friday. Even if a homework assignment has not been given, time should be spent each night reviewing what we did in class that day. Students are responsible for following the formal procedure for make-up work after an absence. Reading scientific papers Writing scientific papers Organization Meeting deadlines Following directions Critical thinking Presenting data in public Take an interest Have students do homework before social time / computer time Encourage study sessions Check day planner for important dates Check grades on my website (Zangle) http://newburyparkhighschool.net/malone/ E-mail me with questions and/or concerns