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EXAM III
Chapters 4,13-16,18
Biotechnology
Biotechnology and review for Final Exam
Biotechnology topics:
1) DNA sequencing Figure 20.1 plus the related text
2) Green Fluorescent Protein = GFP not in textbook
3) PCR Figure 19.5 plus the related text
Cumulative Final Exam:
Wednesday, May 7th, 10:45-12:45,
room 303 Payson-Smith
Do not bring any of your
lecture notes to hand in at the
Final Exam.
1) All living things are composed of cells*
2) All cells come from pre-existing cells*
* The Cell Theory
3) All cells exist in a water environment
4) All cells are bounded by a membrane
5) All cells have similar chemistrys (proteins, proteins,
proteins)
6) DNA is the primary heritable molecule (DNA’s BIG job
is to code for proteins)
7) The concept of gene regulation and totipotency
1) DNA sequencing
Figure 20.1 plus the related text
This is the procedure for DNA sequencing.
BLAST! search at NCBI
http://www.ncbi.nlm.nih.gov/
National Center for Biotechnology Information
BLASTing the genome to search for a match with the
human insulin gene gives the following result:
We can BLAST the fruit fly genome with human insulin
and we find an ortholog:
BLASTing the genome to search for a match with the
human insulin gene gives the following result:
When we BLAST the human genome with the insulin gene, we
Find the insulin gene and we also find two other genes that are
very similar to insulin:
The major hormones that regulate growth in animals are
insulin and insulin-like growth factor (the insulin signaling
system.)
100+ ***
100 *
98
96 *
94 ***
92
90 *
88 *
86 *
84 ****
82 ***
80 **
78 ******
76 *****
74 **
72
70 *
68 ***
66
64 **
62 ****
60 **
58 ***
56
54 *
52
50
48
46 **
44
42
A
_______
A_______
B+
_______
B
MEAN = 77
_______
C
_______
C_______
D-