Synthesis, spectral and antimicrobial studies of thioiminium iodides and amine hydrochlorides (Vol 120, pg 489, 2014) By:Britto, S (Britto, Sebastian)[ 1 ] ; Renaud, P (Renaud, Philippe)[ 2 ] ; Nallu, M (Nallu, Maruthai)[ 3 ] SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY Volume: 127 Pages: 550-550 DOI: 10.1016/j.saa.2014.03.017 Published: JUN 5 2014 View Journal Information Author Information Reprint Address: Britto, S (reprint author) St Josephs Coll, Dept Chem, Tiruchchirappalli 620002, India. Addresses: [ 1 ] St Josephs Coll, Dept Chem, Tiruchchirappalli 620002, India [ 2 ] Univ Bern, Dept Chem & Biochem, CH-3012 Bern, Switzerland [ 3 ] Bharathidasan Univ, Sch Chem, Tiruchchirappalli 620024, Tamil Nadu, India E-mail Addresses:brittoseba@yahoo.co.in Author Identifiers: Publisher PERGAMON-ELSEVIER SCIENCE LTD, THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND Categories / Classification Research Areas:Spectroscopy Web of Science Categories:Spectroscopy Document Information Document Type:Correction Language:English Accession Number: WOS:000336110500068 ISSN: 1386-1425 Other Information IDS Number: AH4PM Cited References in Web of Science Core Collection: 1 Times Cited in Web of Science Core Collection: 0 Defense role of the cocoon in the silk worm Bombyx mori L. By:Pandiarajan, J (Pandiarajan, Jeyaraj)[ 1 ] ; Cathrin, BP (Cathrin, Britto P.)[ 1 ] ; Pratheep, T (Pratheep, Thangaraj)[ 1 ] ; Krishnan, M (Krishnan, Muthukalingan)[ 1 ] RAPID COMMUNICATIONS IN MASS SPECTROMETRY Volume: 25 Issue: 21 Pages: 3203-3206 DOI: 10.1002/rcm.5213 Published: NOV 15 2011 View Journal Information Abstract Silk from the domesticated silk worm Bombyx mori procures foreign body response naturally, so it has been utilized as a biomaterial for decades. In India the prime focus of the sericulture industry is to improve silk production with high quality silk. Naturally, the silk worm builds its cocoon not only with silk proteins, but also with antimicrobial proteins to avoid infection since the cocoon is non-motile and non-feeding. The aim of the present study is to elucidate the antimicrobial proteins that persist in the cocoon of the silk worm Bombyx mori. At the pupal stage, the silk worm cocoon shell extract was prepared from the day of pupation (P0) to the day of natural rupture of the cocoon for the eclosion of moth (NR). Using the cocoon shell extract a microbial susceptibility test was performed by the disc diffusion method against the microbes Escherchia coli, Bacillus cereus, Staphylococcus aureus, Pseudomonas aeruginosa, and Klebsiella pneumoniae. The development of a zone of inhibition against the microbes confirmed the presence of antimicrobial/immunogenic activity of the cocoon shell extract. For further analysis, the cocoon shell extract was subjected to 7-15% sodium dodecyl sulfate/polyacrylamide gel electrophoresis (SDS-PAGE). The protein profile of the cocoon extract revealed the coomassie blue stained bands resolved from the 150 - 15 kDa molecular range. Interestingly, a polypeptide localized at around 29 kDa showed remarkable expressional changes during the development of pupa. To characterize the 29 kDa protein, it was eluted from the gel, digested with trypsin and analyzed by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). The trypsin-digested peptide peaks were analyzed through MASCOT and peptides were matched with the NCBI nr database. The peptides were very well matched with the 18 wheeler protein, which is reported to be responsible for innate immunity, belonging to the Toll family in insects and responsible for cellular mediated immunity. Copyright (C) 2011 John Wiley & Sons, Ltd. Keywords KeyWords Plus:DROSOPHILA TOLL PROTEIN; GENE; FAMILY; MEMBER; 18WHEELER; INSECTS Author Information Reprint Address: Krishnan, M (reprint author) Bharathidasan Univ, Insect Mol Biol Lab, Dept Environm Biotechnol, Sch Environm Sci, Tiruchchirappalli 620024 Addresses: [ 1 ] Bharathidasan Univ, Insect Mol Biol Lab, Dept Environm Biotechnol, Sch Environm Sci, Tiruchchirappalli 620 E-mail Addresses:profmkrish@yahoo.com Funding Funding Agency UGC Grant Number F.4-3/2006 (BSR) 11-73/2008/BSR View funding text Publisher WILEY-BLACKWELL, COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA Categories / Classification Research Areas:Biochemistry & Molecular Biology; Chemistry; Spectroscopy Web of Science Categories:Biochemical Research Methods; Chemistry, Analytical; Spectroscopy Document Information Document Type:Article Language:English Accession Number: WOS:000296428600002 PubMed ID: 22006381 ISSN: 0951-4198 Other Information IDS Number: 840GY Cited References in Web of Science Core Collection: 24 Times Cited in Web of Science Core Collection: 6 Using mass spectrometry to detect buffalo salivary odorantbinding protein and its post-translational modifications By:Rajkumar, R (Rajkumar, R.)[ 1,2,5 ] ; Karthikeyan, K (Karthikeyan, K.)[ 1 ] ; Archunan, G (Archunan, G.)[ 1 ] ; Huang, PH (Huang, P. H.)[ 2 ] ; Chen, YW(Chen, Y. W.)[ 3,4 ] ; Ng, WV (Ng, W. V.)[ 6 ] ; Liao, CC (Liao, C. C.)[ 2 ] RAPID COMMUNICATIONS IN MASS SPECTROMETRY Volume: 24 Issue: 22 Pages: 3248-3254 DOI: 10.1002/rcm.4766 Published: NOV 2010 View Journal Information Abstract A large number of mammalian odorant-binding proteins, which are lipocalins, have been studied. These proteins participate in pen-receptor events by selecting and carrying odorant molecules. The present study aimed at identifying the buffalo salivary odorant-binding protein (sOBP), and to determine its post-translational modification using mass spectrometry. The buffalo salivary 21 kDa protein was initially separated adopting sodium dodecyl sulfate-polyacrylamide gel electrophoresis and it was identified as sOBP with high statistical reliability using liquid chromatography/tandem mass spectrometry (LC/MS/MS) and SEQUEST, for the first time. Further, the post-translationally modified peptides were screened adopting MS/MS. A total of four post-translational modifications, namely glycation at lysine-(59), hydroxylation at lysine-(134), ubiquitination at lysine-(121), and dihydroxylation in lysine-(108), were recorded. Moreover, these modifications have not been identified in buffalo salivary odorant-binding protein. Copyright (C) 2010 John Wiley & Sons, Ltd. Keywords KeyWords Plus:AMINO-ACID-SEQUENCE; PHEROMONEBINDING; BOVINE; CLONING; COMMUNICATION; PURIFICATION; PATHWAY; FLEHMEN;LIGAN D; ESTRUS Author Information Reprint Address: Archunan, G (reprint author) Bharathidasan Univ, Dept Anim Sci, Ctr Pheromone Technol, Tiruchchirappalli 620024, Tamil Nadu, India. Addresses: [ 1 ] Bharathidasan Univ, Dept Anim Sci, Ctr Pheromone Technol, Tiruchchirappalli 620024, Tamil Nadu, India [ 2 ] Natl Yang Ming Univ, Prote Res Ctr, Taipei 112, Taiwan [ 3 ] Natl Tsing Hua Univ, Coll Life Sci, Dept Life Sci, Hsinchu 30013, Taiwan [ 4 ] Natl Tsing Hua Univ, Coll Life Sci, Inst Bioinformat & Struct Biol, Hsinchu 30013, Taiwan [ 5 ] City Univ Hong Kong, Dept Biol & Chem, Prote Core Facil, Hong Kong, Hong Kong, Peoples R China [ 6 ] Natl Yang Ming Univ, Inst Biotechnol Med, Taipei 112, Taiwan E-mail Addresses:archunan@bdu.ac.in; ccliao@ym.edu.tw Funding Funding Agency Grant Number DBT UGC-SAP, Government of India National Science Council of Taiwan View funding text Publisher JOHN WILEY & SONS LTD, THE ATRIUM, SOUTHERN GATE, CHICHESTER PO19 8SQ, W SUSSEX, ENGLAND Categories / Classification Research Areas:Biochemistry & Molecular Biology; Chemistry; Spectroscopy Web of Science Categories:Biochemical Research Methods; Chemistry, Analytical; Spectroscopy Document Information Document Type:Article Language:English Accession Number: WOS:000284023400004 PubMed ID: 20972998 ISSN: 0951-4198 Other Information IDS Number: 677VZ Cited References in Web of Science Core Collection: 42 Times Cited in Web of Science Core Collection: 9 Detection of alpha(2u)-globulin and its bound putative pheromones in the preputial gland of the Indian commensal rat (Rattus rattus) using mass spectrometry By:Rajkumar, R (Rajkumar, R.)[ 1,2 ] ; Ilayaraja, R (Ilayaraja, R.)[ 1 ] ; Liao, CC (Liao, C. C.)[ 2 ] ; Archunan, G (Archunan, G.)[ 1 ] ; Achiraman, S (Achiraman, S.)[ 3 ] ; Prakash, S (Prakash, S.)[ 5 ] ; Ng, WV (Ng, W. V.)[ 4 ] ; Tsay, YG (Tsay, Y. G.)[ 2 ] RAPID COMMUNICATIONS IN MASS SPECTROMETRY Volume: 24 Issue: 6 Pages: 721-728 DOI: 10.1002/rcm.4437 Published: MAR 2010 View Journal Information Abstract The role of pheromones and pheromone-binding proteins in the laboratory rat has been extensively investigated. However, we have previously reported that the preputial gland of the Indian commensal rat produces a variety of pheromonal molecules and preputial glands would seem to be the predominant source for pheromonal communication. The presence of pheromone-binding proteins has not yet been identified in the preputial gland of the Indian commensal rat; therefore, the experiments were designed to unravel the alpha(2u)-globulin (alpha 2u) and its bound volatiles in the commensal rat. Total preputial glandular proteins were first fractionated by sodium dodecyl sulfate/polyacrylamide gel electrophoresis (SDS-PAGE) and subsequently analyzed by mass spectrometry. Further, we purified alpha 2u and screened for the presence of bound pheromonal molecules with the aid of gas chromatography/mass spectrometry (GC/MS). A novel alpha 2u was identified with a high score and this protein has not been previously described as present in the preputial gland of Indian commensal rats. This novel alpha 2u was then characterized by tandem mass spectrometry (MS/MS). Peptides with m/z values of 969, 1192, 1303 and 1876 were further fragmented with the aid of MS/MS and generated de novo sequences which provided additional evidence for the presence of alpha 2u in the preputial gland. Finally, we identified the presence of farnesol 1 and 2 bound to alpha 2u. The present investigation confirms the presence of alpha 2u (18.54 kDa) in the preputial gland of the Indian commensal rat and identifies farnesol 1 and 2 as probably involved in chemo-communication by the Indian commensal rat. Copyright (C) 2010 John Wiley & Sons, Ltd. Keywords KeyWords Plus:MICE Author Information Reprint Address: Archunan, G (reprint author) Bharathidasan Univ, Dept Anim Sci, Ctr Pheromone Technol, Tiruchchirappalli 620024, Tamil Nadu, India. Addresses: [ 1 ] Bharathidasan Univ, Dept Anim Sci, Ctr Pheromone Technol, Tiruchchirappalli 620024, Tamil Nadu, India [ 2 ] Natl Yang Ming Univ, Prote Res Ctr, Inst Biochem & Mol Biol, Taipei 112, Taiwan [ 3 ] Bharathidasan Univ, Dept Environm Biotechnol, Tiruchchirappalli 620024, Tamil Nadu, India [ 4 ] Natl Yang Ming Univ, Inst Biotechnol Med, Taipei 112, Taiwan [ 5 ] Indian Inst Sci, Dept Biochem, Mol Biophys Unit, Bangalore 560012, Karnataka, India E-mail Addresses:archunan@bdu.ac.in Funding Funding Agency Grant Number Commonwealth Commission, UK Heiwa Nakajima Foundation, Japan UGC UGC-SAP DST-FIST, Government of India View funding text Publisher WILEY-BLACKWELL, COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA Categories / Classification Research Areas:Biochemistry & Molecular Biology; Chemistry; Spectroscopy Web of Science Categories:Biochemical Research Methods; Chemistry, Analytical; Spectroscopy Document Information Document Type:Article Language:English Accession Number: WOS:000276111100003 PubMed ID: 20169559 ISSN: 0951-4198 Other Information IDS Number: 575YX Cited References in Web of Science Core Collection: 41 Times Cited in Web of Science Core Collection: 3 Cyanobacterial chlorophyll as a sensitizer for colloidal TiO2 By:Kathiravan, A (Kathiravan, A.)[ 1 ] ; Chandramohan, M (Chandramohan, M.)[ 2 ] ; Renganathan, R (Renganathan, R.)[ 1 ] ; Sekar, S (Sekar, S.)[ 2 ] SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY Volume: 71 Issue: 5 Pages: 1783-1787 DOI: 10.1016/j.saa.2008.06.031 Published: JAN 2009 View Journal Information Abstract Chlorophyll has been extracted from cyanobacteria. The adsorption of chlorophyll on the surface of colloidal TiO2 through electrostatic interaction was observed. The apparent association constant (K,,p) of chlorophyll-TiO2 obtained from absorption spectra is 3.78 x 10(4) M-1. The K-app value of chlorophyll-TiO2 as determined from fluorescence spectra is 1.81 x 10(4) M-1, which matches well with that determined from the absorption spectra changes. These data indicate that there is an interaction between chlorophyll and colloidal TiO2 nanoparticle surface. The dynamics of photoinduced electron transfer from chlorophyll to the conduction band of colloidal TiO2 nanoparticle has been observed and the mechanism of electron transfer has been confirmed by the calculation of free energy change (Delta G(et)) by applying Rehm-Weller equation as well as energy level diagram. Lifetime measurements gave the rate constant (k(et)) for electron injection from the excited state chlorophyll into the conduction band of TiO2 is 4.2 x 10(8) s(-1). (C) 2008 Elsevier B.V. All rights reserved. Keywords Author Keywords:TiO2 colloid; Fluorescence quenching; Chlorophyll KeyWords Plus:PHOTOINDUCED INTERACTION; ELECTRON-TRANSFER; PARTICULATE SYSTEMS; PHOTO-SENSITIZATION; FLASH-PHOTOLYSIS; PHOTOSYSTEM-II; HYPOCRELLINB; CYANINE DYE; PHOTOSENSITIZATION; SEMICONDUCTOR Author Information Reprint Address: Renganathan, R (reprint author) Bharathidasan Univ, Sch Chem, Tiruchchirappalli 620024, India. Addresses: [ 1 ] Bharathidasan Univ, Sch Chem, Tiruchchirappalli 620024, India [ 2 ] Bharathidasan Univ, Dept Biotechnol, Tiruchchirappalli 620024, India E-mail Addresses:rrengas@gmail.com Funding Funding Agency Grant Number 01(2217)/08/EMR-11 CSIR, Government of India dt.06/05/2008 Department of Science and Technology, New Delhi, Government of India View funding text Publisher PERGAMON-ELSEVIER SCIENCE LTD, THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND Categories / Classification Research Areas:Spectroscopy Web of Science Categories:Spectroscopy Document Information Document Type:Article Language:English Accession Number: WOS:000261900000026 PubMed ID: 18678524 ISSN: 1386-1425 Other Information IDS Number: 386RJ Cited References in Web of Science Core Collection: 33 Times Cited in Web of Science Core Collection: 9 An investigation on electron transfer quenching of zinc(II) meso-tetraphenylporphyrin (ZnTPP) by colloidal TiO(2) By:Kathiravan, A (Kathiravan, A.)[ 1 ] ; Renganathan, R (Renganathan, R.)[ 1 ] SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY Volume: 71 Issue: 3 Pages: 1106-1109 DOI: 10.1016/j.saa.2008.03.006 Published: DEC 1 2008 View Journal Information Abstract The interaction of zinc(II) rneso-tetraphenylporphyrin (ZnTPP) with colloidal TiO(2) was studied by absorption, steady state and time-resolved fluorescence spectroscopy. The quenching was found to obey the Stern-Volmer equation and the corresponding Stern-Volmer plots were linear in the range of quencher concentration used 0-5 x 10(-4) M. The bimolecular quenching rate constants (k(q)) were 20.5 x 10(10) M(-1) s(-1) (steady-state) and 2.85 x 10(10) M(-1) s(-1) (time resolved). The quenching process is suggested to involve electron transfer from the ZnTPP to TiO(2) considering the experimental evidences obtained. (C) 2008 Elsevier B.V. All rights reserved. Keywords Author Keywords:ZnTPP; Fluorescence quenching; Colloidal TiO(2) KeyWords Plus:FLASH-PHOTOLYSIS; HYPOCRELLINB; PHOTOSENSITIZATION; SEMICONDUCTOR; PHOTOOXIDATION; DYES; ABSORPTION;THYM INE Author Information Reprint Address: Renganathan, R (reprint author) Bharathidasan Univ, Sch Chem, Tiruchchirappalli 620024, Tamil Nadu, India. Addresses: [ 1 ] Bharathidasan Univ, Sch Chem, Tiruchchirappalli 620024, Tamil Nadu, India E-mail Addresses:rrengas@gmail.com Funding Funding Agency Grant Number DST (Government of India) View funding text Publisher PERGAMON-ELSEVIER SCIENCE LTD, THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND Categories / Classification Research Areas:Spectroscopy Web of Science Categories:Spectroscopy Document Information Document Type:Article Language:English Accession Number: WOS:000261118400062 PubMed ID: 18424228 ISSN: 1386-1425 Other Information IDS Number: 375MQ Cited References in Web of Science Core Collection: 23 Times Cited in Web of Science Core Collection: 21 NIR-FT Raman and FT-IR spectral investigations of the nonlinear optical chromophore p-bromoacetanilide By:Jothy, VB (Jothy, V. Bena); Vijayakumar, T (Vijayakumar, T.); Jayakumar, VS (Jayakumar, V. S.); Udayalekshmi, K (Udayalekshmi, K.); Ramamoorthy, K (Ramamoorthy, K.); Joe, IH (Joe, I. Hubert) JOURNAL OF RAMAN SPECTROSCOPY Volume: 38 Issue: 9 Pages: 1148-1158 DOI: 10.1002/jrs.1743 Published: SEP 2007 View Journal Information Abstract Vibrational spectral analysis of the hydrogen-bonded nonlinear optical (NLO) material p-bromo acetanilide (PBA) was carried out using NIR-FT-Raman and FT-IR spectroscopy. Ab initio molecular orbital computations were performed at HF/6-31G (d) level to derive equilibrium geometry, vibrational wavenumbers, intensities and first hyperpolarizability. The lowering of the imino stretching wavenumbers suggests the existence of strong intermolecular N-H center dot center dot center dot O hydrogen bonding, which was substantiated by the natural bond orbital (NBO) analysis. The vibrational spectra confirm that the charge-transfer interaction between the -NHCOCH3 group and Br through phenyl ring is responsible for simultaneous strong IR and Raman activation of the ring mode 8a. Vibrational analysis indicates that the lowering of stretching wavenumbers of methyl group due to electronic effects simultaneously caused by induction and hyperconjugation is due to the presence of the oxygen atom. The presence of blue-shifting H-bonds of CH stretching wavenumbers, simultaneous activation of carbonyl stretching mode, the strong activity of low-wavenumber H-bond stretching vibrations and the role of intramolecular charge transfer in making the molecule NLO active have been analyzed on the basis of the vibrational spectral features. Copyright (C) 2007 John Wiley & Sons, Ltd. Keywords Author Keywords:Ab initio quantum chemical computations; intramolecular charge transfer; interand intramolecular ionic hydrogen bonds; first hyperpolarizability; natural bond orbitals KeyWords Plus:AB-INITIO CALCULATIONS; CENTER-DOT-O; INFRAREDSPECTRA; VIBRATIONAL-SPECTRA; HYDROGEN-BONDS; MATRIXISOLATION; CRYSTAL; SPECTROSCOPY; ACETANILIDE; DERIVATIVES Author Information Reprint Address: Joe, IH (reprint author) Mar Ivanios Coll, Dept Phys, Ctr Mol & Biophys Res, Thiruvananthapuram 695015, Kerala, India. Addresses: [ 1 ] Mar Ivanios Coll, Dept Phys, Ctr Mol & Biophys Res, Thiruvananthapuram 695015, Kerala, India [ 2 ] Womens Christian Coll, Dept Phys, Nagercoil 629001, Tamil Nadu, India [ 3 ] Bharathidasan Univ, Sch Phys, Tiruchchirappalli 620024, Tamil Nadu, India E-mail Addresses:hubertjoe@sancharnet.in Publisher JOHN WILEY & SONS LTD, THE ATRIUM, SOUTHERN GATE, CHICHESTER PO19 8SQ, W SUSSEX, ENGLAND Categories / Classification Research Areas:Spectroscopy Web of Science Categories:Spectroscopy Document Information Document Type:Article Language:English Accession Number: WOS:000249470600011 ISSN: 0377-0486 Other Information IDS Number: 210QL Cited References in Web of Science Core Collection: 55 Times Cited in Web of Science Core Collection: 44 Catalytic and biological activities of Ru(III) mixed ligand complexes containing N,O donor of 2-hydroxy-1naphthylideneimines By:Venkatachalam, G (Venkatachalam, G); Ramesh, R (Ramesh, R) SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY Volume: 61 Issue: 9 Pages: 2081-2087 DOI: 10.1016/j.saa.2004.08.008 Published: JUL 2005 View Journal Information Abstract A series of stable low spin Ru(III) complexes of the type [RuX2(EPh3)(2)(L)] (where E = P or As; X = Cl or Br; L = mono basic bidentate Schiff bases) have been synthesized and were characterized by analytical, spectral and electrochemical data. A distorted octahedral geometry has been proposed for all the complexes. These complexes catalyze oxidation of primary alcohols and secondary alcohol with high yields in the presence of N-methylmorpholine-N-oxide (NMO). The ruthenium(III) Schiff base complexes show growth inhibitory activity against the bacteria Staphylococcus aureus (209p) and E. coli ESS (2231). (c) 2004 Elsevier B.V. All rights reserved. Keywords Author Keywords:ruthenium(III) Schiff base complexes; spectra; redox properties; catalytic oxidation; antibacterial activity KeyWords Plus:SCHIFF-BASE LIGANDS; TRANSFER RADICAL POLYMERIZATION; RUTHENIUM(II) COMPLEXES; REDOX PROPERTIES;ANTIFUNGAL ACTIVITY; SALEN COMPLEXES; TRIPHENYLARSINE; TRIPHENYLPHOSPHINE; COORDINATION; OXIDATION Author Information Reprint Address: Ramesh, R (reprint author) Bharathidasan Univ, Dept Chem, Tiruchchirappalli 620024, Tamil Nadu, India. Addresses: [ 1 ] Bharathidasan Univ, Dept Chem, Tiruchchirappalli 620024, Tamil Nadu, India E-mail Addresses:ramesh_bdu@yahoo.com Publisher PERGAMON-ELSEVIER SCIENCE LTD, THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND Categories / Classification Research Areas:Spectroscopy Web of Science Categories:Spectroscopy Document Information Document Type:Article Language:English Accession Number: WOS:000229841100015 PubMed ID: 15911395 ISSN: 1386-1425 Other Information IDS Number: 936FO Cited References in Web of Science Core Collection: 49 Times Cited in Web of Science Core Collection: 19