Microarrays
Microarrays

Used to observe which genes are expressed in cells

1-DNA segments are taken of specific genes and placed
on a DNA “chip” or slide

2-mRNA from tissue samples are then converted back to
DNA and each type of tissue is then “labeled” usually with
a color-cDNA

3-Hybridize the genes-mix the cDNA with the gene
segments on the slide, if they match the color will appear
and that gene is expressed. If there is no match, the
color will not appear. If there is a slight match, a color will
appear but will not be the color the cDNA was labeled
with.
Microarray movie clip

http://www.youtube.com/watch?v=VNsThMNjKhM

https://www.youtube.com/watch?v=2RQnY1NrkhA

http://learn.genetics.utah.edu/content/labs/microarray/
How is this helpful?
 Can
determine genes involved in
cancer
 Can make a “pre-test” for cancer
 Can determine genes expressed in
two types of tissues under different
conditions
 **Also used to determine if you have
been infected with a virus…
DNA Microarray Lab Results
Gene Description
Gene is expressed more in lung cancer cells than in normal lung cells
Gene is expressed in both lung cancer cells and normal lung cells
Gene is not expressed in in either lung cancer cells or normal lung
cells
Gene is expressed more in normal lung cells than in lung cancer cells
Color of Spot
Pink
Purple
Colorless
Blue