1 Think about… 22.1 Basic concepts of respiration 22.2 Site of respiration 22.3 Aerobic respiration 22.4 Anaerobic respiration 22.5 Relationship between respiration and photosynthesis Recall ‘Think about…’ Summary concept map 2 Food like corn can provide energy for the body. 3 The sugar in it can also undergo fermentation (發酵) to produce an alcohol. 4 The alcohol can be used as a fuel to power vehicles. 5 This ‘biofuel’ (生物燃料) releases less air pollutants upon burning. 6 1 How does our body obtain energy from the food we eat 7 2 How is alcohol produced from corn by fermentation 8 3 The sugar in corn is made by photosynthesis. What is the relationship between respiration and photosynthesis 9 22.1 Basic concepts of respiration What is respiration? 10 22.1 Basic concepts of respiration What is respiration? • when food is burnt, it reacts with oxygen (oxidation 氧化): heat O2 light CO2 + H2O glucose 11 22.1 Basic concepts of respiration What is respiration? • when food is burnt, it reacts with oxygen (oxidation 氧化): - one step reaction - takes place anywhere - no enzyme involved - fast and violent reaction 12 22.1 Basic concepts of respiration What is respiration? • when food is burnt, it reacts with oxygen (oxidation 氧化): - all stored energy is released at once - energy is released in the forms of heat and light 13 22.1 Basic concepts of respiration What is respiration? • the large amount of heat released in burning kills living cells organisms undergo respiration (呼吸作用) the process by which organisms release energy from food through the controlled oxidative breakdown of food 14 22.1 Basic concepts of respiration What is respiration? • glucose is the most common substrate heat ATP O2 CO2 + H2O glucose (in the cell) 15 22.1 Basic concepts of respiration What is respiration? • respiration: - a series of reactions - takes place in all living cells all the time - controlled by many enzymes - slow and gradual reactions 16 22.1 Basic concepts of respiration What is respiration? • respiration: - stored energy is released gradually - energy released is used to form ATP while some is lost as heat 17 22.1 Basic concepts of respiration What is respiration? • overall equation: glucose CO2 O2 enzymes H2O energy 18 22.1 Basic concepts of respiration What is the role of ATP? • as energy carrier energy released from respiration ATP phosphorylation (磷酸化) P ADP 19 22.1 Basic concepts of respiration What is the role of ATP? • as energy carrier energy released from respiration releases energy to cells ATP breakdown ADP P 20 22.1 Basic concepts of respiration What is the role of ATP? • ATP releases energy for metabolic activities: - cell division - muscle contraction - transmission of nerve impulse 21 22.1 Basic concepts of respiration What is the role of ATP? • ATP releases energy for metabolic activities: - synthesis of biomolecules amino acids protein - absorption of food molecules or minerals by active transport 22 22.1 Basic concepts of respiration Types of respiration 1 Aerobic respiration (需氧呼吸) • requires oxygen • glucose is completely broken down • a large amount of energy is released 23 22.1 Basic concepts of respiration Types of respiration 2 Anaerobic respiration (缺氧呼吸) • does not require oxygen • glucose is only partly broken down • much less energy is released • products are different from aerobic respiration 24 22.1 Basic concepts of respiration 1 Respiration is the process by which organisms release chemical energy from food through the controlled oxidative breakdown of food . 25 22.1 Basic concepts of respiration 2a Similarities between burning and respiration: i Both are an oxidative process. 26 22.1 Basic concepts of respiration 2a Similarities between burning and respiration: ii Both need oxygen . 27 22.1 Basic concepts of respiration 2a Similarities between burning and respiration: iii Carbon dioxide and water are produced. 28 22.1 Basic concepts of respiration 2b Differences between burning and respiration: i Burning is a one-step reaction, while respiration is a series of reactions. 29 22.1 Basic concepts of respiration 2b Differences between burning and respiration: ii Burning takes place anywhere while respiration takes place only inside cells . 30 22.1 Basic concepts of respiration 2b Differences between burning and respiration: iii Burning involves no enzymes while respiration is controlled by many enzymes. 31 22.1 Basic concepts of respiration 2b Differences between burning and respiration: iv Burning is a fast and violent reaction while respiration is a slow and gradual reaction. 32 22.1 Basic concepts of respiration 2b Differences between burning and respiration: v In burning, stored energy is released at once while in respiration, stored energy is released gradually . 33 22.1 Basic concepts of respiration 2b Differences between burning and respiration: vi In burning, energy is released in the forms of heat and light while in respiration, energy released is used to form ATP while some is lost as heat . 34 22.1 Basic concepts of respiration 3 ATP acts as an energy carrier . When it breaks down into ADP and a phosphate group, energy is released and is readily usable by cells for metabolic activities . 35 22.1 Basic concepts of respiration 4a Aerobic respiration requires oxygen . Glucose is completely broken down into carbon dioxide and water . A large amount of energy is released. 36 22.1 Basic concepts of respiration 4b Anaerobic respiration does not require oxygen. Glucose is partly broken down. Much less energy is released and different products are formed. 37 22.2 Site of respiration • some reactions occur in the cytoplasm, some in the mitochondria 38 22.2 Sites of respiration Adaptive features of a mitochondrion 3D model • bound by a double membrane • outer membrane controls the movement of substances outer membrane 39 22.2 Sites of respiration Adaptive features of a mitochondrion • inner membrane is highly folded provides a large surface area to pack more enzymes inner membrane 40 22.2 Sites of respiration Adaptive features of a mitochondrion • mitochondrial matrix (基質) provides a fluid medium for reactions to take place • it also contains enzymes mitochondrial matrix 41 22.2 Sites of respiration Adaptive features of a mitochondrion • most energy in food is released inside mitochondria active cells contain many mitochondria muscle cells 42 22.2 Sites of respiration 22.1 Examination of the structure of mitochondria 1 Examine the photomicrograph or live cell image of a mitochondrion. 43 22.2 Sites of respiration 22.1 Examination of the structure of mitochondria 2 Identify various structures of the mitochondrion and draw a high power diagram. 44 22.2 Sites of respiration 1 Some reactions of respiration occur in the cytoplasm and some in the mitochondria . 45 22.2 Sites of respiration 2 Structure of a mitochondrion adapted to respiration: a The inner membrane is highly folded provides a large surface area to pack more enzymes that catalyse the reactions of respiration 46 22.2 Sites of respiration 2 Structure of a mitochondrion adapted to respiration: b There is mitochondrial matrix contains enzymes for respiration 47 22.2 Sites of respiration 2 Structure of a mitochondrion adapted to respiration: b There is mitochondrial matrix provides a fluid medium for reactions to take place 48 22.3 Aerobic respiration • takes place in the presence of oxygen • three stages: glycolysis (糖酵解) Krebs cycle (克雷伯氏循環) oxidative phosphorylation (氧化磷酸化) 49 22.3 Aerobic respiration Glycolysis • occurs in the cytoplasm • does not require oxygen • two main steps: 1 Breakdown of glucose to triose phosphate 2 Oxidation of triose phosphate to pyruvate (丙酮酸鹽) 50 22.3 Aerobic respiration Glycolysis 1 Breakdown of glucose to triose phosphate glucose (6-C) 2 ATP 2 ADP + P 2 triose phosphate (3-C) 51 22.3 Aerobic respiration Glycolysis 2 Oxidation of triose phosphate to pyruvate 2 triose phosphate (3-C) 2 NAD 4 ADP + 4 P 2 NADH 4 ATP 2 pyruvate (3-C) 52 22.3 Aerobic respiration Glycolysis 2 Oxidation of triose phosphate to pyruvate 2 triose phosphate (3-C) 2 NAD 4 ADP + 4 P 2 NADH as hydrogen 4 ATP donor 2 pyruvate (3-C) 53 22.3 Aerobic respiration Glycolysis • overall equation: 2 NAD glucose (6-C) 2 ADP + 2 P 2 NADH 2 pyruvate (3-C) 2 ATP transported to mitochondrion 54 22.3 Aerobic respiration • before entering the Krebs cycle: 2 NAD pyruvate (3-C) 2 NADH acetyl-CoA (2-C) CO2 coenzyme A (CoA) 55 22.3 Aerobic respiration Kerbs cycle • occurs in the mitochondrial matrix • two main steps: 1 Combination of acetyl-CoA with 4-C compound 2 Regeneration of 4-C compound 56 22.3 Aerobic respiration Kerbs cycle 1 Combination of acetyl-CoA with 4-C compound acetyl-CoA (2-C) 4-C compound CoA 6-C compound 57 22.3 Aerobic respiration Kerbs cycle 2 Regeneration of 4-C compound 4-C compound 6-C compound ATP ADP + P FADH FAD 2 CO2 3 NAD 3 NADH 58 22.3 Aerobic respiration Kerbs cycle • each glucose molecule generates two pyruvate molecules a total of six NADH, two FADH and two ATP are formed 59 22.3 Aerobic respiration Oxidative phosphorylation • occurs on the inner membrane of the mitochondrion • two main steps: 1 Regeneration of NAD and FAD 2 Formation of ATP 60 22.3 Aerobic respiration Oxidative phosphorylation 1 Regeneration of NAD and FAD intermembrane space inner membrane mitochondrial matrix 61 22.3 Aerobic respiration Oxidative phosphorylation 1 Regeneration of NAD and FAD H hydrogen carrier NADH NAD 62 22.3 Aerobic respiration Oxidative phosphorylation 1 Regeneration of NAD and FAD H H NADH NAD FADH FAD H2O H + O 63 22.3 Aerobic respiration Oxidative phosphorylation 2 Formation of ATP H H NADH NAD ADP+P ATP H FADH FAD + O H2O 64 22.3 Aerobic respiration Oxidative phosphorylation 2 Formation of ATP • one NADH can generate three ATP • one FAD can generate two ATP 65 22.3 Aerobic respiration Oxidative phosphorylation 2 Formation of ATP Glycolysis: 2 NADH Pyruvate to acetyl-CoA: 2 NADH Krebs cycle: 6 NADH 2 FADH = 6 ATP = 6 ATP = 22 ATP Total: 34 ATP 66 22.3 Aerobic respiration Let’s summarize the overall process of aerobic respiration. 67 22.3 Aerobic respiration 68 22.3 Aerobic respiration Overall equation: C6H12O6 enzymes 6 O2 6 CO2 6 H2O 38 ATP 69 22.3 Aerobic respiration 22.2 Video Investigation of carbon dioxide production in a living mouse Carbon dioxide turns lime water milky. The air breathed out by a mouse is passed through lime water to see whether the mouse has produced carbon dioxide or not. 70 22.3 Aerobic respiration 22.2 Investigation of carbon dioxide production in a living mouse 1 Place a mouse on a glass plate and cover it with a bell jar. Smear the edge of the bell jar with vaseline to make the bell jar airtight. 2 Set up the apparatus as shown. Note the colours of the solutions in flasks A, B and C. 71 22.3 Aerobic respiration 22.2 Investigation of carbon dioxide production in a living mouse air in bell jar KOH lime water A B to suction pump lime water C 72 22.3 Aerobic respiration 22.2 Investigation of carbon dioxide production in a living mouse 3 Turn on the suction pump to draw a stream of air through the apparatus for 30 minutes. Record any colour changes in the flasks. 73 22.3 Aerobic respiration 22.2 Results and discussion The potassium hydroxide solution in flask A does not change its colour. It absorbs carbon dioxide in the air. This prevents carbon dioxide from entering the bell jar. The lime water in flask B remains clear. This shows that the air entering the bell jar contains no carbon dioxide. 74 22.3 Aerobic respiration 22.2 Results and discussion The lime water in flask C turns milky after some time. This shows that carbon dioxide is present in the air leaving the bell jar, which must have been given out by the living mouse. 75 22.3 Aerobic respiration 22.3 Video Investigation of carbon dioxide production in germinating seeds Seeds germinate when conditions are favourable. During germination, their respiration greatly speeds up to release more energy to support growth. 76 22.3 Aerobic respiration 22.3 Investigation of carbon dioxide production in germinating seeds Hydrogencarbonate indicator can be used to detect any carbon dioxide produced by germinating seeds. Its colour changes with the concentration of carbon dioxide. 77 22.3 Aerobic respiration 22.3 Investigation of carbon dioxide production in germinating seeds Concentration Colour of of CO2 hydrogencarbonate indicator > 0.03% Yellow ~ 0.03% Red/orange < 0.03% Purple 78 22.3 Aerobic respiration 22.3 Investigation of carbon dioxide production in germinating seeds 1 Set up the apparatus as shown. Leave the tubes for a few hours. A soaked germinating seeds moist cotton wool wire gauze hydrogencarbonate 79 indicator 22.3 Aerobic respiration 22.3 Investigation of carbon dioxide production in germinating seeds 1 Set up the apparatus as shown. Leave the tubes for a few hours. B boiled seeds moist cotton wool wire gauze hydrogencarbonate 80 indicator 22.3 Aerobic respiration 22.3 Investigation of carbon dioxide production in germinating seeds 2 Record any colour changes in the hydrogencarbonate indicator of tubes A and B. 81 22.3 Aerobic respiration 22.3 Results and discussion The hydrogencarbonate indicator in tube B remains red while that in tube A turns yellow after a few hours. These results show that germinating seeds give out carbon dioxide. 82 22.3 Aerobic respiration 22.4 Video Investigation of heat production in a living mouse 1 Set up the differential air thermometer as shown. 83 22.3 Aerobic respiration 22.4 Investigation of heat production in a living mouse graph paper clip arm A arm B test tube coloured liquid in U-shaped capillary tube cotton wool 84 22.3 Aerobic respiration 22.4 Investigation of heat production in a living mouse 2 Open the clips on both sides of the thermometer. Wait until the coloured liquid levels in arms A and B become the same. 3 Close the clips. Record any changes in the liquid levels after 10 minutes. 85 22.3 Aerobic respiration 22.4 Results and discussion At the end of the experiment, the liquid level in arm A rises and the liquid level in arm B falls. This is because the mouse releases heat which warms up the air in the test tube on the side where it is. The expansion of air inside the tube increases the pressure. This causes the liquid level in arm B to fall. The results therefore 86 show that a living mouse releases heat. 22.3 Aerobic respiration 22.4 Results and discussion The clips are opened before the experiment starts to equalize the pressure on both sides of the U-shaped capillary tube. The cotton wool is used as an insulator to prevent heat loss so that the result is more easily observed. 87 22.3 Aerobic respiration 22.5 Video Design an investigation of heat production in germinating seeds From Practical 22.4, we know that a living mouse produces heat when it carries out respiration. Do germinating seeds produce heat during respiration as well? Try to design and perform an investigation to find out the answer. 88 22.3 Aerobic respiration Different stages of aerobic respiration: 1 Glycolysis occurs in cytoplasm . • Glucose is split into two molecules of triose phosphate using energy from ATP 89 22.3 Aerobic respiration Different stages of aerobic respiration: 1 Glycolysis occurs in cytoplasm . • Triose phosphate is oxidized to pyruvate ; NADH and ATP are formed 90 22.3 Aerobic respiration Different stages of aerobic respiration: 1 Glycolysis occurs in cytoplasm . • Net amount of ATP formed: 2 91 22.3 Aerobic respiration Different stages of aerobic respiration: 2 Conversion of pyruvate to acetyl-CoA occurs in mitochondrial matrix . • Pyruvate is converted to acetyl-CoA; carbon dioxide and NADH are formed 92 22.3 Aerobic respiration Different stages of aerobic respiration: 2 Conversion of pyruvate to acetyl-CoA occurs in mitochondrial matrix . • Net amount of ATP formed: 0 93 22.3 Aerobic respiration Different stages of aerobic respiration: 3 Krebs cycle occurs in mitochondrial matrix. • Acetyl-CoA combines with a 4-C compound to form a 6-C compound 94 22.3 Aerobic respiration Different stages of aerobic respiration: 3 Krebs cycle occurs in mitochondrial matrix. • The 6-C compound is oxidized step by step to regenerate 4-C compound; carbon dioxide, NADH, FADH and ATP are formed 95 22.3 Aerobic respiration Different stages of aerobic respiration: 3 Krebs cycle occurs in mitochondrial matrix. • Net amount of ATP formed: 2 96 22.3 Aerobic respiration Different stages of aerobic respiration: 4 Oxidative phosphorylation occurs in inner membrane of mitochondrion. • NADH and FADH lose hydrogen . They are oxidized to regenerate NAD and FAD 97 22.3 Aerobic respiration Different stages of aerobic respiration: 4 Oxidative phosphorylation occurs in inner membrane of mitochondrion. • The oxidation of NADH and FADH releases energy to form ATP by phosphorylation 98 22.3 Aerobic respiration Different stages of aerobic respiration: 4 Oxidative phosphorylation occurs in inner membrane of mitochondrion. • Hydrogen is finally accepted by oxygen to form water 99 22.3 Aerobic respiration Different stages of aerobic respiration: 4 Oxidative phosphorylation occurs in inner membrane of mitochondrion. • Net amount of ATP formed: 36 100 22.4 Anaerobic respiration • does not require oxygen • all reactions occur in the cytoplasm only • starts with glycolysis but will not proceed to the Kerbs cycle and oxidative phosphorylation 101 22.4 Anaerobic respiration How does anaerobic respiration occur? 102 22.4 Anaerobic respiration 1 Formation of lactic acid (乳酸) in muscles glucose (6-C) 2 ADP + 2 P 2 NAD 2 ATP 2 NADH 2 pyruvate (3-C) glycolysis 103 22.4 Anaerobic respiration 1 Formation of lactic acid (乳酸) in muscles 2 pyruvate (3-C) 2 NADH 2 NAD 2 lactic acid (3-C) 104 22.4 Anaerobic respiration 1 Formation of lactic acid (乳酸) in muscles • produces only two ATP through glycolysis • simple and can supply energy quickly 105 22.4 Anaerobic respiration 1 Formation of lactic acid (乳酸) in muscles • the formation of lactic acid by anaerobic respiration is called lactic acid fermentation (乳酸發酵) • overall equation: glucose energy (2 ATP) 2 lactic acid 106 22.4 Anaerobic respiration 1 Formation of lactic acid (乳酸) in muscles • anaerobic respiration provides additional energy in a very short time allows muscles to contract more powerfully and at a higher rate 107 22.4 Anaerobic respiration 1 Formation of lactic acid (乳酸) in muscles • lactic acid formed builds up in muscles and causes pain muscle fatigue (肌肉疲勞) 108 22.4 Anaerobic respiration amount of O2 breathed in 1 Formation of lactic acid (乳酸) in muscles rest exercise recovery rest time • after doing strenuous exercise, our breathing remains deep for some time 109 22.4 Anaerobic respiration amount of O2 breathed in 1 Formation of lactic acid (乳酸) in muscles oxygen debt (氧債) rest exercise recovery rest time • extra oxygen is used to break down lactic acid 110 22.4 Anaerobic respiration amount of O2 breathed in 1 Formation of lactic acid (乳酸) in muscles oxygen debt (氧債) rest exercise recovery rest time • lactic acid is broken down to CO2 and water or converted to glycogen 111 22.4 Anaerobic respiration 2 Formation of ethanol and carbon dioxide in yeast glucose (6-C) 2 ADP + 2 P 2 NAD 2 ATP 2 NADH 2 pyruvate (3-C) glycolysis 112 22.4 Anaerobic respiration 2 Formation of ethanol and carbon dioxide in yeast 2 pyruvate (3-C) 2 NADH 2 CO2 2 NAD 2 ethanol (2-C) 113 22.4 Anaerobic respiration 2 Formation of ethanol and carbon dioxide in yeast • the formation of ethanol by anaerobic respiration is called alcoholic fermentation (酒精發酵) • overall equation: glucose energy (2 ATP) 2 ethanol 2 CO2 114 22.4 Anaerobic respiration 22.6 Video Demonstration of anaerobic respiration in germinating seeds Germinating seeds can carry out anaerobic respiration when there is no oxygen. It is shown by removing oxygen from the set-up. The gas released is tested with hydrogencarbonate indicator. 115 22.4 Anaerobic respiration 22.6 Demonstration of anaerobic respiration in germinating seeds The production of carbon dioxide in the absence of oxygen indicates the occurrence of anaerobic respiration. 116 22.4 Anaerobic respiration 22.6 Demonstration of anaerobic respiration in germinating seeds 1 Put the seeds into a boiling tube and then place a piece of wire gauze. Fill the tube with paraffin oil. boiling tube wire gauze soaked germinating 117 seeds 22.4 Anaerobic respiration 22.6 Demonstration of anaerobic respiration in germinating seeds 2 Invert the tube and insert it into a container of paraffin oil. Do not trap any air between the seeds and the oil. paraffin oil 118 22.4 Anaerobic respiration 22.6 Demonstration of anaerobic respiration in germinating seeds 3 Leave the tube for a few hours and record the change in oil level. Collect the gas as pull the plunger to shown. collect the gas syringe gas collected rubber 119 tubing 22.4 Anaerobic respiration 22.6 Demonstration of anaerobic respiration in germinating seeds 4 Bubble the gas through hydrogencarbonate indicator as shown. push the plunger to gas force out the gas collected hydrogencarbonate indicator 120 22.4 Anaerobic respiration 22.6 Results and discussion The colour of the indicator changes from red/orange to yellow. This shows that the gas produced is carbon dioxide. The germinating seeds can respire anaerobically when oxygen is absent. 121 22.4 Anaerobic respiration Applications of anaerobic respiration • the brewing of beer makes use of the alcohol formed when yeast ferments the sugar in barley (大麥) 122 22.4 Anaerobic respiration Applications of anaerobic respiration • the brewing of wine makes use of the alcohol formed when yeast ferments the sugar in grape juice 123 22.4 Anaerobic respiration Applications of anaerobic respiration • CO2 formed by alcoholic fermentation in yeast helps raise dough in bread-making 124 22.4 Anaerobic respiration Applications of anaerobic respiration • yoghurt contains lactic acid formed by anaerobic respiration in bacteria 125 22.4 Anaerobic respiration Applications of anaerobic respiration • lactic acid formed by anaerobic respiration in bacteria helps coagulate milk to form cheese 126 22.4 Anaerobic respiration Applications of anaerobic respiration • ethanol formed by the fermentation of sugar in crops can be used as a fuel to power vehicles 127 22.4 Anaerobic respiration 1 Anaerobic respiration in skeletal muscles: Glucose undergoes glycolysis and is oxidized to pyruvate . NADH and ATP are formed in the process. Pyruvate is reduced to lactic acid by NADH . 128 22.4 Anaerobic respiration 2 Anaerobic respiration in muscles provides additional energy in a very short time for muscle contraction . 129 22.4 Anaerobic respiration 3 During strenuous exercise, the lactic acid formed by anaerobic respiration accumulates in muscles and causes muscle fatigue . 130 22.4 Anaerobic respiration 3 We keep breathing deeply after exercise to take in extra oxygen . It is used to remove all lactic acid by breaking it down to carbon dioxide and water or converting it to glycogen . 131 22.4 Anaerobic respiration 4 Anaerobic respiration in yeast: Glucose undergoes glycolysis and is oxidized to pyruvate. NADH and ATP are formed in the process. Pyruvate is reduced to ethanol by NADH. Carbon dioxide is released in the process. 132 22.4 Anaerobic respiration 5a Similarities of aerobic and anaerobic respiration: Both release energy from the oxidative breakdown of organic substances . 133 22.4 Anaerobic respiration 5a Similarities of aerobic and anaerobic respiration: Both transfer energy to the energy carrier ATP , and some energy is lost as heat . 134 22.4 Anaerobic respiration 5a Similarities of aerobic and anaerobic respiration: Both consist of a number of reactions controlled by enzymes . 135 22.4 Anaerobic respiration 5b Differences between aerobic and anaerobic respiration: Aerobic respiration occurs in cytoplasm and mitochondria while anaerobic respiration occurs only in cytoplasm . 136 22.4 Anaerobic respiration 5b Differences between aerobic and anaerobic respiration: Aerobic respiration requires oxygen but anaerobic respiration does not. 137 22.4 Anaerobic respiration 5b Differences between aerobic and anaerobic respiration: In aerobic respiration, organic substances are completely broken down into carbon dioxide and water . 138 22.4 Anaerobic respiration 5b Differences between aerobic and anaerobic respiration: But in anaerobic respiration, organic substances are partly broken down to form lactic acid or ethanol and carbon dioxide. 139 22.4 Anaerobic respiration 5b Differences between aerobic and anaerobic respiration: In aerobic respiration, 38 ATP per glucose molecule is formed (a larger amount of energy is released). 140 22.4 Anaerobic respiration 5b Differences between aerobic and anaerobic respiration: In anaerobic respiration, 2 ATP per glucose molecule is formed (a much smaller amount of energy is released). 141 22.4 Anaerobic respiration 6 Alcoholic fermentation in yeast is used in brewing beer and wine , raising dough in bread-making and producing ethanol as a biofuel. 142 22.4 Anaerobic respiration 6 Lactic acid fermentation in bacteria is used in making yoghurt and cheese. 143 22.5 Relationship between respiration and photosynthesis • exchange of molecules between respiration and photosynthesis bridges the flow of energy from the environment to organisms 144 22.5 Relationship between respiration and photosynthesis Exchange of molecules light H2O H2O photochemical reactions oxidative phosphorylation chloroplast mitochondrion O2 O2 145 22.5 Relationship between respiration and photosynthesis Exchange of molecules CO2 CO2 Calvin cycle Krebs cycle glycolysis glucose pyruvate 146 22.5 Relationship between respiration and photosynthesis Flow of energy oxygen glucose photosynthesis CO2 water respiration 147 22.5 Relationship between respiration and photosynthesis In energy transformation • ATP acts as the energy carrier energy stored in organic compounds ATP light energy energy for cellular metabolism ADP +P ADP +P ATP 148 22.5 Relationship between respiration and photosynthesis In energy transformation • ATP acts as the energy carrier energy stored in photosynthesis organic ADP compounds +P ATP light energy energy for cellular metabolism ADP +P respiration ATP 149 22.5 Relationship between respiration and photosynthesis Differences between respiration and photosynthesis: 1 Site of occurrence: Respiration occurs in all living cells while photosynthesis occurs in chloroplast-containing cells 150 22.5 Relationship between respiration and photosynthesis Differences between respiration and photosynthesis: 2 Type of metabolism: In respiration, catabolism occurs. Organic food is broken down by oxidation to release energy 151 22.5 Relationship between respiration and photosynthesis Differences between respiration and photosynthesis: 2 Type of metabolism: In photosynthesis, anabolism occurs. Organic food is built up by reduction to store energy 152 22.5 Relationship between respiration and photosynthesis Differences between respiration and photosynthesis: 3 Energy change: In respiration, chemical energy in food is converted to ATP and heat 153 22.5 Relationship between respiration and photosynthesis Differences between respiration and photosynthesis: 3 Energy change: In photosynthesis, light energy from the sun is converted to chemical energy in food 154 22.5 Relationship between respiration and photosynthesis Differences between respiration and photosynthesis: 4 Cyclic reactions: In Krebs cycle of respiration, carbon dioxide is removed from the substrate and ATP , NADH and FADH are formed 155 22.5 Relationship between respiration and photosynthesis Differences between respiration and photosynthesis: 4 Cyclic reactions: In Calvin cycle of photosynthesis, carbon dioxide is fixed into the cycle by a 5-C compound and NADPH and ATP are used 156 22.5 Relationship between respiration and photosynthesis Differences between respiration and photosynthesis: 5 Formation of ATP: In respiration, ATP is formed in glycolysis, Krebs cycle and oxidative phosphorylation 157 22.5 Relationship between respiration and photosynthesis Differences between respiration and photosynthesis: 5 Formation of ATP: In photosynthesis, ATP is formed in photophosphorylation 158 22.5 Relationship between respiration and photosynthesis Differences between respiration and photosynthesis: 6 Hydrogen donor: In respiration, NADH and FADH are the hydrogen donors while in photosynthesis, water is the hydrogen donor 159 22.5 Relationship between respiration and photosynthesis Differences between respiration and photosynthesis: 7 Final hydrogen acceptor: In respiration, oxygen is the final hydrogen acceptor while in photosynthesis, a 3-C compound in Calvin cycle is the final hydrogen acceptor 160 1 How does our body obtain energy from the food we eat? Our body releases energy stored in food by respiration. The energy is used to form ATP which drives all cellular activities. 161 2 How is alcohol produced from corn by fermentation? Sugar in corn is converted to ethanol by alcoholic fermentation in yeast. 162 3 The sugar in corn is made by photosynthesis. What is the relationship between respiration and photosynthesis? Respiration and photosynthesis together allow the flow of energy in the ecosystem. 163 Respiration is requires oxygen does not require oxygen anaerobic aerobic respiration respiration oxidative breakdown of food 164 oxidative breakdown of food releases chemical energy mostly as heat some stored in ATP 165 aerobic respiration anaerobic respiration both involve glycolysis occurs in cytoplasm 166 glycolysis if aerobic, then followed by Kerbs cycle occur in oxidative phosphorylation mitochondria 167 glycolysis if anaerobic, then followed by formation of lactic acid occur in formation of ethanol and carbon dioxide cytoplasm 168