Summer School in NCSR Demokritos
Summer 2005
Methods in micro – nano technology and nanobiotechnology
Organizer: National Center for Scientific Research “Demokritos”
Participating Institutes:
Microelectronics,
Radioisotopes and Radiodiagnostic Products,
Physical Chemistry
Information: www.imel.demokritos.gr,
Contact: Evangelos Gogolides, evgog@imel.demokritos.gr
Rational:
Modern Research takes advantage of Micro and Nanotechnology developments.
Merging areas of research (Nanobiotechnology) demand interdisciplinary skills.
Necessary for researchers from Life Sciences, Chemistry, and Engineering to
acquire skills in Micro and Nanotechnologies.
Target: Establish common language between the various disciplines-promote
interdisciplinary research
Content: Five (5) day intensive summer school
Offers: classroom and laboratory experience on:
micro and nano-technology processes / materials / applications
Targeted in: Nanobiotechnology
Who should attend:
Group leaders involved in molecular biology or biotechnology,
Post Doctoral Fellows
Graduate students with Engineering, Science or Life Science background,
All those who wish to apply micro-technology in their research.
Maximum number of registrants 20 persons.
Fees: 1000 Euro (accommodation NOT included)
Dates: 6-10 June 2005.
Synergies with other schools in Greece and Nano2life:
Back to back with Practical Course on Advanced techniques in molecular biology
organized by FORTH/ICEHT, Date: 13-18 June 2005
http://mb_school.iceht.forth.gr
A small reduction in the fee for those taking both seminars is being considered.
Syllabus 1:
Lecture 1:
Introduction to micro and nano-technology and
nanobiotechnology
Lecture 2:
Patterning technologies (lithography and plasma etching)
for Si, glass, and plastic substrates in the fabrication of
analytical/bioanalytical microdevices
Laboratory 1: Fabrication of microfluidic devices on plastic substrates by
novel lithographic techniques.
Syllabus 2:
Laboratory 2: Fabrication of plastic Capillary Electrophoresis Devices by
Lithography and plasma etching techniques.
Lecture 3:
Principles of Integrated Biosensing Devices
Laboratory 3: Operation of a lab-on-a-chip optical device using model
assays and real time measurements
Monolithic silicon optocouplers
PMMA Capillaries
Syllabus 3:
Lecture 4:
Patterning of biomolecules and other biological
substances
Laboratory 4a:
Part A: Fabrication of protein microarrays using
biocompatible photoresist-based lithography.
Laboratory 4b:
Part B. Fluorescence detection of protein arrays.
Twelve rows of different protein spots fabricated
in 12 succesive lithographic steps
Fluorescence picture of the rabbit γ-globulins and biotinylated-BSA spot arrays
after a 2 h immunoreaction with a mixture of AF 546 labeled streptavidin (red
spots) and AF 488 labeled anti-rabbit IgG antibody (green spots). The spot size is
approximately 4 μm.
Syllabus 4:
Lecture 5:
Biomolecules as building blocks of molecular
electronics
Laboratory 5:
Nano-patterning by electron beam lithography
Lecture 6:
Protein and DNA arrays: fabrication, detection and
applications.
Laboratory 6:
Demonstrating a capillary fluoroimmunosensor
Syllabus 5:
Lecture 7:
Drug Delivery, Liposomes, Dendrimers, Cyclodextrins
Laboratory 7: Dynamic light scattering and Z-potential measurements,
Video enhanced optical microscopy of Liposomes and
Atomic Force Microscopy
Atomic Force Microscopy
Formation of DNA nanoparticles of ~40 nm diameter
Syllabus 6:
Laboratory 7: Dynamic light scattering optical microscopy
5μm
5μm
5μm
PC-CHOL-ODPG /
DHP Liposomes
PEG 0%
PEG 5%
PEG 15%
5μm
5μm
5μm
10
100
1000
r (nm)
10000 100000
5μm
Liposome-liposome interactions
Correlation of Optical Microscopy and Dynamic Light Scattering results
Syllabus 7:
Laboratory 8: Drug inclusion monitoring in situ by NMR spectroscopy
X-ray diffraction characterisation of drug inclusion in
Cyclodextrins and 3-D visualisation
NH2
6
(OH)n
ppm (t1)
5.00
4.50
4.00
3.50