Myeloproliferative Neoplasms 2015 Robert J. Jacobson MD, FACP , FRCP(C). Florida Cancer Specialists Affiliate Professor of Biological Sciences Charles E. Schmidt School of Medicine Florida Atlantic University, Boca Raton, FL 1 Stem Cells and Hematopoietic Differentiation • Hematopoietic stem cells capable of – Self-renewal – Differentiation • Differentiation and proliferation controlled by molecular signals – Contact with stromal cells in bone marrow – Growth factors 2 Myeloproliferative Neoplasms (MPN) - Clonal Hematopoietic stem cell diseases - Overproduction of terminally differentiated cells of the myeloid lineage • Ph+ Chronic Myeloid Leukemia (CML) • Polycythemia Vera (P. Vera) • Essential Thrombocythemia (ET) • Primary Myelofibrosis (PMF) 3 CML Epidemiology and Etiology In the US, there were 4,870 cases in 2010 and an expected 5,430 cases in 2012 15% of all adult leukemias Incidence increases significantly with age – Median age: ~ 67 years – Prevalence increasing due to current therapy – Most patients present in CP Majority of CML-related deaths due to progression to AP/BC – 50% of CML patients are asymptomatic at diagnosis Risk factors – Radiation exposure 4 Most CML Patients Are Diagnosed in the Chronic Phase Chronic Phase 80% Blast Phase 10% 5 CML with Philadelphia Chromosome t (9; 22) 6 CML Pathogenesis: Philadelphia (Ph) Chromosome CML first cancer demonstrated to have underlying genetic abnormality • Associated with Ph chromosome Result of translocation between chromosomes 9 and 22 Detected in approx. 95% of patients with CML 7 Clinical Presentation of Ph+ CML 8 Treatment of CML • Imatinib (Gleevec), developed by Dr. Brian Druker at Oregon Health & Sciences University and Novartis pharmaceutical company in mid-1990. • Also found to be effective in other blood cancers and GIST by inhibiting different TK proteins. • World-wide sales now $28 billion in the 10 years from 2001-2011. • Now 4 drugs, second generation tyrosine kinase inhibitors available to treat CML. • Drugs continue throughout life. 9 Imatinib Mechanism of Action 10 Treatment of CML • 4 Targeted drugs which block the action of tyrosine kinase (TKIs): – Imatinib (Gleevec) – Dastinib (Sprycel) – Nilotinib (Tasigna) – Bosutinib (Bosulif) • Side Effects – Skin rash – Fluid retention – Diarrhea – Vascular thrombosis • Omacetaxine (Synribo) or Homoharringtonine (alkaloid) approved use when 2 TKIs have failed 11 Event-Free Survival and Survival Without AP/BC on First-Line Imatinib 12 Survival Rates for Stem Cell Transplantation 13 ‘Inverted Iceberg’ Schematic of CML burden and reduction over time. 14 CML Treatment Cessation of TKIs • If relapse occurs, it develops within the first 6 months after the treatment stopped • About 40%—50% of patients have no recurrence if their initial leukemic burden was low and they had been on treatment for a long duration (> 10 years) • Ongoing studies investigating second generation TKIs e.g. nilotinib and achieving more rapid MMR > 4 – 5 logs • Current recommendation is to continue with TKI treatment for life • Patients may not be able to tolerate TKI and alternative drug chosen 15 Ph Negative MPN Majority of the patients have the gene mutation JAK2V617F • Polycythemia vera • Essential Thrombocythemia • Primary Myelofibrosis 16 Diagnosis of Polycythemia Vera Major Criteria 1. Hgb level > 18.5 g/dL in men, >16.5 g/dL in women 2. Presence of the JAK2V617F mutation (in 95% of patients) Minor Criteria 1. Bone marrow biopsy showing hypercellularity 2. Serum Epo level below normal range 3. Endogenous erythroid colony formation in vitro PV is likely if: A. Both major criteria and at least 1 minor criterion are met, or B. First major criterion and at least 2 minor criteria are met Epo = erythropoietin; Hgb = hemoglobin; JAK = Janus-associated kinase; PV= polycythemia vera 17 Clinical Features of P. Vera • Fever, weight loss • Pruritis • Plethoric facies • Cyanosed digits • Splenomegaly • Thrombotic events • Transformation to leukemia • Myelofibrosis 18 JAK2V617F and STAT Pathways 19 Treatment of Polycythemia Vera (PV) • • • • • Control and maintain Hct levels <45% Manage disease-related complications of PV Phlebotomy to maintain Hct levels <45% Low-dose aspirin in appropriate patients Hydroxyurea or IFNα as first-line cytoreductive therapy at any age • Patients with inadequate response to or intolerance of HU use Ruxolitinib (Jakifi) JAK2 inhibitor Hct = hematocrit; HU = hydroxyurea; IFNα = interferon- α 20 Essential Thrombocythemia • Elevated platelet count often in excess of 1 million/dL, RBC and WBC normal • JAK2V617F mutation in 50% of patients • Thrombosis/hemorrhagic complications • Splenomegaly can develop, especially if transformation to Myelofibrosis (MF) • Low dose aspirin, anegrelide, hydroxyurea used • Clinical trials underway with JAK2 inhibitors • Patients can live >10 years 21 JAK2 and Other Mutations in MPN 22 Myelofibrosis with Myeloid Metaplasia • JAK2 mutation in 60% of patients • Myelofibrosis is a clonal hematologic malignancy with reactive marrow fibrosis • Primary myelofibrosis, or PMF, entered the scientific discourse in 1879 with varied nomenclature –Agnogenic myeloid metaplasia –Myeloid metaplasia with myelofibrosis –Chronic idiopathic myelofibrosis • Patients with an antecedent diagnosis of PV or ET are diagnosed with PPV-MF or PET-MF 23 Peripheral Blood and Bone Marrow in Myelofibrosis Red blood cells, tear-drop shape Bone marrow fibrosis A leukoerythroblastic blood smear Reticulin stain of marrow 24 Understanding Myelofibrosis • Myelofibrosis can be risk stratified by: low risk intermediate risk 1 & 2 high risk –Bone marrow fibrosis –Symptom burden –Splenomegaly –Cytopenias • Although the exact cause of myelofibrosis is unknown, multiple mechanisms are key features of the disease –Genetic mutations: JAK2, CALR and MPL –Excessive production of cytokines –Increased JAK1 signaling 25 Cytokines Play a Key Role in Myelofibrosis 26 Overactive JAK-STAT Signaling is Present in all Patients with Myelofibrosis 27 Clinical Relevance of the Progressive Pathology of Myelofibrosis 28 Diagnosing primary Myelofibrosis: All major criteria plus ≥2 minor WHO criteria are required for diagnosis 29 Risk Stratification in Myelofibrosis 30 Burden of Disease: Symptoms of Myelofibrosis 31 Examining the causes of mortality in PMF 32 Diagnosing PPV-MF or PET-MF 33 Geyer and Mesa, ASH 2014 34 Myelofibrosis Status Agent ↓ ↓ ↑ Spleen Const. HB Sympt . +++ +++ - Approved Ruxolintinib (MF) (INCB18424) Phase III (PV) Phase III (MF) Pacritnib+++ (SB1518) Phase III (MF) Momelotinib +++ Phase II (CYT387) (PV/ET) Polycythemia Vera ↑ ↓ ↓ SURV Phleb Const. Sympt . ++ +++ +++ +++ - Unk +++ - Unk Unk Unk ↓ Vasc Event s Unk Unk 35 Summary & Conclusions • Myeloproliferative neoplasms are characterized by distinct and specific genetic findings and clinical presentations • The translocation t(9:22) and mutation JAK2V617F are responsible for the abnormal clonal production of cells derived from the myeloid lineage • Therapies are now available directed against the specific tyrosine kinases (BCR-ABL) or genetic pathways (JAK-STAT) that cause the uncontrolled marrow cellular growth 36