• Little Calumet River Near O’Brien Lock • • • • • • and Dam Rotenone sample area = 2.6 miles of channel or 150 surface acres of water Eleven nets (total = 16,800 feet) set May 20, run and reset May 21 and 22, and pulled May 23 Water samples for eDNA analysis collected morning of May 20 DC electrofishing for sport fish for a total of 20 hours on May 12 No Asian carp captured or seen No Asian carp DNA detected Trammel and gill net locations for sampling conducted May 20 – May 23, 2010 by commercial fishermen Ron Brown and John Ahrling. Nets were 300-600 feet long x 5-12 ft. deep with bar mesh sizes ranging from 2.5 -3.5 inches. JA – 01 JA – 02 JA – 03 JA – 04 RB - 03 RB - 05 RB - 07 RB - 01 RB - 02 RB - 06 RB - 04 Rotenone Fish Recovery Summary Number of individuals Number of species Hybrid groups Total weight recovered (pounds) Standing stock (lbs./acre) 67,224 38 2 97,720 565 Commercial Net Sample Totals Species Number Percent Common carp 561 59.3 Freshwater drum 174 18.4 Gizzard shad 148 15.6 Channel catfish 33 3.5 Black buffalo 16 1.7 Goldfish 5 0.5 Carp x Goldfish hybrid 5 0.5 Yellow bullhead 2 0.2 Yellow bass 1 0.1 White perch 1 0.1 Electrofishing Sample Totals Species Number Percent Alewife 7 1.1 Black buffalo 2 0.3 Black bullhead 1 0.2 Bluegill 11 1.7 Bluntnose minnow 44 7 Channel catfish 17 2.7 Common carp 216 34.1 Emerald shiner 61 9.6 Fathead minnow 9 1.4 Freshwater drum 5 0.8 Gizzard shad 76 12 Golden shiner 1 0.2 44 3 Goldfish Green sunfish Species Number Percent Rainbow trout 1 0.2 Rock bass 3 0.5 46 7.3 Weather loach 1 0.2 White bass 2 0.3 White crappie 1 0.2 White perch 24 3.8 White sucker 7 1.1 Yellow bass 2 0.3 Yellow bullhead 2 0.3 7 Yellow perch 4 0.6 0.5 Total number 633 100 Round goby Rotenone Fish Recovery Species List Species Gizzard shad Common carp Ghost shiner Emerald shiner Alewife Freshwater drum Bluntnose minnow Goldfish Round goby Channel catfish Rock bass Pumpkinseed White perch Bluegill Spotfin shiner Golden shiner Yellow bullhead Black crappie Fathead minnow White crappie Number Percent 22298 33.2 9820 14.6 6945 10.3 4342 6.5 3485 5.2 3178 4.7 2374 3.5 2293 3.4 2109 3.1 1959 2.9 1244 1.8 1098 1.6 1059 1.6 783 1.2 464 0.7 434 0.6 366 0.5 333 0.5 325 0.5 319 0.5 Species White sucker Yellow perch Black bullhead Largemouth bass Spottail shiner Black buffalo Orangespotted sunfish White bass Goldfish x Common carp hybrid Green sunfish Smallmouth bass Grass carp Warmouth Smallmouth buffalo Yellow bass Brown bullhead Flathead catfish Grass pickerel Hybrid sunfish Number Percent 319 0.5 268 0.4 264 0.4 219 0.3 213 0.3 205 0.3 174 0.3 82 0.1 53 0.1 49 0.1 45 0.1 43 0.1 18 <0.1 12 <0.1 10 <0.1 8 0.0 8 <0.1 2 <0.1 2 <0.1