Today’s Plan: 9/16/09
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Bellwork:
Watch Video clip (10 mins)
Finish Mutations and Operons (15 mins)
Read/Pair/ Share about diabetes articles (15 mins) Read 1
insulin article. Your seat partner will do the other.
Compare info and do journal
– Why do diabetics need insulin?
– What does insulin do?
– What sources of insulin are available to diabetics?
– What are the risks of using animal-derived insulin?
• Notes on Recombinant DNA and restriction enzymes (20
mins)
• Agriculture article/questions (the rest of the period)
• Pack/Wrap-up (last few mins of class)
Today’s Plan: 9/17/09
• Bellwork: Ag applications article and questions
(20 mins)
• Notes on RFLP and other technology (30 mins)
• RFLP Activity (20 mins)
• The rest of the period:
– Gene Therapy Article and questions
• Pack/Wrap-up (last few mins of class)
Today’s Plan: 9/18/09
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Bellwork: Q&A (10 mins)
Vocab quiz and Unit 2 Test (as needed)
If you finish early, work on missing tasks
Pack/Wrap-up (last few mins)
Today’s Plan: 9/21/09
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Bellwork: Blue Diamond #5 (20 mins)
Portfolio Update (10 mins)
Go over Friday’s test (35 mins)
Finish test corrections and missing work
(the rest of class)
• Pack/Wrap-up (last few mins of class)
Today’s Plan: 9/24/08
• Bellwork: Re-cap on crime scene (10 mins)
• Crime Scene analysis (the rest of the
period)
• Pack/Wrap-up (last few mins)
Today’
Recombinant DNA
• DNA can be manipulated to contain certain
genes. Manipulations are performed by cutting
DNA, inserting what we want, and inserting the
DNA back into a cell.
• A manipulated piece of DNA, that contains
“spliced” pieces is called recombinant DNA
• The recombinant DNA comes from cloning
vectors-carrier DNA molecules that can be cut
and spliced with new genes
• Most cloning vectors in biotechnology are
bacterial plasmids
Transplanting Genes
• 1st –cloning vector is removed from bacterial
cells
• 2nd-restriction enzymes that recognize specific
base sequences on the DNA cut the plasmid at
pre-determined sites, creating sticky ends
• 3rd-the donor gene is inserted, closing the plasmid
back into its ring shape
• 4th-the new, recombinant DNA is inserted back
into the bacterium. When the bacteria goes
through binary fission, the donor gene is cloned
too. The bacteria is now called a transgenic
organism
Checking for Donor Gene
Uptake
• As you know, not all laboratory exercises work,
and scientists need a way to check to see if their
transgenic bacteria picked up the donor gene.
• To test this, an antibiotic resistant gene is often
also transferred with the desired donor gene.
• We can then grow the bacteria up on a plate
containing the antibiotic in the agar. Only
resistant bacteria survive, and these will also
contain the donor gene that we’re looking for.
Other Uses of Restriction
Enzymes
• Digesting DNA fragments for
Fingerprinting.
• DNA is digested, loaded into a gel, and
you end up with bands of DNA
• Bands are characteristic of each person,
and can be used as a “Fingerprint” (also
known as RFLP= Restriction Fragment
Length Polymorphism)
Uses of DNA Technology
• Gene Therapy-fixing genes with mistakes
in an adult organism
• PCR-Copying mass quantities of DNA
fragments
• Cloning-using nuclear switches of stem
cells to create identical organisms
• Human Genome Project (HGP)Sequencing and Mapping all of the genes
in the human genome.
Test Topics
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Steps to creating transgenic organisms
Purpose of each step
Steps of cloning
Uses of stem cells
Pros/Cons of human gene therapy
Steps to RFLP analysis
Uses for RFLP