Name: _____________________
November 12, 2014
Gr.12 Biology Unit 2: Mechanisms of Inheritance (DNA – Ch.25 & 26)
Section A: Multiple Choice (17 marks)
1. _____The base adenine is always paired with ___.
A.
B.
C.
D.
Adenine
Guanine
Cytosine
Thymine
2.
_____The sequence of one strand of DNA is 5’ CGCATT 3’. The sequence of the complementary
strand would be
A.
B.
C.
D.
E.
5’ GCGTAA 3’
5’ GCGUAA 3’
5’ ATACGG 3’
3’ GCGTAA 5’
3’ GCGUAA 5’
3. _____In contrast to DNA, RNA:
A.
B.
C.
D.
E.
F.
Is single stranded
Contains uracil
Contains deoxyribose
Is a double helix
Only A and B are true
Only A and C are true
4. _____ The enzyme that “unzips” DNA is:
A.
B.
C.
D.
E.
DNA helicase
DNA convertase
DNA primase
DNA ligase
RNA primase
5. _____ Prokaryotic plasmid DNA differs from a eukaryotic DNA in that plasmid DNA:
A.
B.
C.
D.
Contains different complementary base-pairs
Cannot be used for cloning
Is circular
Guanine pairs with adenine
6. _____ The four nitrogenous bases incorporated into tRNA are
A.
B.
C.
D.
E.
Guanine, Uracil, Thymine, Alanine
Adenine, Guanine, Cytosine, Thymine
Cytosine, Uracil, Adenine, Guanine
Thymine, Uracil, Guanine, Cytosine
Inosine, Guanine, Adenine, Cytosine
7. _____ The process of producing a new complementary DNA strand from a DNA template is
called __.
A.
B.
C.
D.
replication
transcription
translation
duplication
8. _____ The process of producing an amino acid polymer (polypeptide) from a mRNA template is
called _____________.
A.
B.
C.
D.
replication
transcription
translation
duplication
9.
A.
B.
C.
D.
_____ The following is NOT true of tRNA
produced in the nucleus
DNA serves as its template
Contains codons
Carries amino acids to the ribosome
10. _____ The biggest difference between Prokaryotes compared with Eukaryotes in gene
expression is that for Prokaryotes:
A.
B.
C.
D.
Mitochondria are located outside the nucleus
RNA is not used in gene expression
Transcriptional control is the main method of control of gene expression
Post-translational control is the main method of control of gene expression
11. _____If the mRNA sequence is CGGUUA, which of the following represents the complimentary
tRNA:
A.
B.
C.
D.
GCCAAT
ACCGGT
ACCGGU
GCCAAU
12. _____ If the original DNA sequence is GGG TTA, the protein produced will be:
A.
B.
C.
D.
Gly-Leu
Pro-Leu
Pro-Asn
Gly-Ile
13. ______The “start” codon in a DNA codes for the amino acid(s):
a. Tryptophan
b. Threonine
c. Methionine
d. Arginine
14. Gene mutations are mainly caused by:
a. Errors in replication
b. Mutagens
c. Transposons
d. Nonfunctional proteins
15. Which of the following is NOT true of translation:
a. Enzymes are required at each step
b. Start codon is AUG
c. There are three binding sites: A, P and E
d. The four steps are initiation, elongation, propagation and termination
16. Which of the following in NOT true of ex vivo gene therapy:
a. Uses pancreatic stem cells
b. The genetic engineering takes place outside the person
c. Is used to treat bubble boy syndrome
d. Uses retroviruses to deliver normal genes to stem cells
17. Which of the following is NOT true of the human genome:
a. There are approximately 3.2 billion base pairs
b. There are approximately 20 000 genes
c. 99% of the human genome directly for amino acid sequences
d. Single nucleotide polymorphisms never have an effect on protein coding
Section B: Short Answer (21 marks)
1. (6) Transcribe and translate the following DNA.
5’ CCCTGGTTUCGTGTAGAA 3’
2. (3) Explain the difference between how point and frameshift mutations work, as well as which
on is more likely to result in a nonfunctional protein.
3. (2) What is a mutagen? Give an example.
4. (2) What are transposons and what is their most common function?
5. (3) What is comparative genomics, and why is it useful? Explain one application.
6. (5) Compare and contrast DNA and RNA (5 marks)
Section C: Long Answer (10 marks)
Answer ONE of the following questions. You may not answer the question that you already responded to in your
DNA technology response assignment.
1) Do you agree or disagree with the use of recombinant DNA technology? Explain how this technology works and
what risks and benefits are associated with it?
2) Do you agree or disagree with the use of gene therapy to treat disease (such as the bubble boy syndrome or
Cystic Fibrosis)? Explain how gene therapy works and what risks and benefits are associated with it.
3) Explain how the Hershey and Chase experiment identified DNA as the hereditary genetic material.
4) Explain the process of DNA replication in detail. Be sure to include all steps and enzymes required.
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