CELLS! CELLS! PROKARYOTIC vs. EUKARYOTIC (prokaryotes vs. eukaryotes) Prokaryotes (bacteria) Simple organism Smaller Cytoplasm Cell Membrane Cell Wall Circular DNA (“nucleoid region”) Some have flagella or pili No membrane-bound organelles Harvest light (photosynthesis, decompose/recycle dead organisms, some cause disease, involved in important industrial processes Eukaryotes ★ Complex organism ★ Larger ★ Cytoplasm ★ Cell membrane ★ Cell Wall (plants, fungi and some protists) ★ Some have flagella or cilia ★ Have membrane-bound organelles CELLS! PROKARYOTE vs. EUKAROTE Common Features CELLS! PROKARYOTE vs. EUKAROTE Electron Micrograph http://www.stolaf.edu/people/giannini/cell/nuc.htm EUKARYOTES ★ ★ Greek = “true nucleus” Compartmentalized with organelles (membranebound structures) ★ Eukaryotic cells include: plant, animal, fungi, protist PLANT vs. ANIMAL EUKARYOTES PLANT vs. Electron Micrograph ANIMAL EUKARYOTIC ORGANELLES PLANT ANIMAL ★ Cell Wall ★ Centriole ★ Chloroplast ★ Cilia or Flagella ★ Large central vacuole ★ Many small vacuoles ★ Cell/Plasma Membrane ★ Cell/Plasma Membrane ★ Cytoplasm/Cytosol ★ Cytoplasm/Cytosol ★ Mitochondria ★ Mitochondria ★ Ribosomes ★ Ribosomes ★ Nucleus ★ Nucleus ★ Nucleolus ★ Nucleolus ★ Nuclear Envelope/Membrane ★ Nuclear Envelope/Membrane ★ Rough Endoplasmic Reticulum (E.R.)★ Rough Endoplasmic Reticulum ★ Smooth E.R. ★ Smooth E.R. ★ Lysosomes ★ Lysosomes ★ Golgi Body/Apparatus ★ Golgi Body/Apparatus ★ Cytoskeleton ★ Cytoskeleton EUKARYOTIC ORGANELLES Structure Nucleus Nucleolus Endoplasmic Reticulum (Smooth & Rough) Golgi Apparatus Lysosomes Mitochondria Chloroplasts (Plant) Description Function EUKARYOTIC ORGANELLES Structure Centrioles (Animal) Cytoskeleton Flagella/Cilia (Animal, fungi and some protists) Central Vacuole (Plant) Cell Wall (Plant) Cytoplasm/Cytosol Ribosomes Description Function EUKARYOTIC ORGANELLES THE NUCLEUS ★ Latin = “kernel” or “nut” ★ First described by Robert Brown (1831) ★ Most eukaryotic cells contain one central nucleus ★Fungi have many nuclei ★ Contains Nucleolus (where synthesis of ribosomal RNA takes place) ★ Nucleus protected by Nuclear Envelope/Membrane (which contains nuclear pores) Structure Description Function Nucleus Usually spherical surrounded by double membrane that contains chromosomes Control center of cell; directs protein synthesis and cell reproduction. Site of genes for ribosomal RNA (rRNA) synthesis Assembles ribosomes Nucleolus EUKARYOTIC ORGANELLES THE NUCLEUS http://www.cartage.org.lb/en/themes/Sciences/Zoo logy/AnimalPhysiology/Anatomy/AnimalCellStruct ure/Nucleus/Nucleus.htm http://www.frontiers-ingenetics.org/en/pictures/nucleus_1.jpg EUKARYOTIC ORGANELLES THE ENDOPLASMIC RETICULUM ★ Endoplasmic = “within the cytoplasm” ★ Reticulum = Latin for “a little net” ★ ROUGH E.R.: Contain many ribosomes, which appear pebbly or “rough”, and are destined to be exported from the cell; synthesizes proteins. ★ SMOOTH E.R.: Relatively few or no ribosomes; may contain enzymes which can 1) carry out lipid synthesis (testes, intestine, and brain) or; 2) carry out detoxification of drugs (liver) Structure Endoplasmic Reticulum (Smooth & Rough) Description Network of highly folded phospholipid membranes within the cytoplasm Function Forms compartments and vesicles; participates in protein and lipid synthesis; transports synthesized proteins EUKARYOTIC ORGANELLES THE ENDOPLASMIC RETICULUM http://www.ccs.k12.in.us/chsBS/kons/kons/eukaryotic%2 0cell/cytoplasm_and_its_associated_str_files/image002.j pg http://academic.brooklyn.cuny.edu/biology/bio4fv/page/r ougher.htm EUKARYOTIC ORGANELLES THE GOLGI BODY/APPARATUS ★ Flattened stacks of tubular membranes ★ Smooth, membranous structure located near the middle of the cell ★ Receives proteins and lipids from the E.R. ★ Abundant in glandular cells ★ Modifies and sorts proteins ★ Packages proteins into membrane-bound structures, called vesicles, to be sent to appropriate destinations ★ Similar to a post office Structure Golgi Body/Apparatus Description Flattened stacks of tubular membranes Function Sorts, packages and transports proteins for export from the cell EUKARYOTIC ORGANELLES THE GOLGI BODY/APPARATUS http://www.bu.edu/histology/i/20303ooa.jpg http://media-2.web.britannica.com/eb-media/52/116252004-9615DB80.jpg EUKARYOTIC ORGANELLES THE LYSOSOME ★ Membrane-bounded digestive vesicles ★ Arise from the Golgi Apparatus ★ Contain degrading enzymes which break down old organelles ★ Enzymes also function to eliminate harmful or foreign cells through phagocytosis ★ Include microbodies (glyoxysome, peroxisome) Structure Lysosome Description Vesicles derived from Golgi apparatus that contain digestive enzymes Function Digests worn-out organelles and foreign or harmful cells; acts like a “garbage disposal”; plays a role in cell death (“suicide sacs”) EUKARYOTIC ORGANELLES THE LYSOSOME http://www.cartage.org.lb/en/themes/sciences/zoology/A nimalPhysiology/Anatomy/AnimalCellStructure/Lysosom es/lysosome.jpg http://www.stolaf.edu/people/giannini/cell/lys/autophag.j pg EUKARYOTIC ORGANELLES EUKARYOTIC ORGANELLES THE MITOCHONDRION ★ Plural = Mitochondria ★ Peanut-shaped with outer membrane and highly folded inner membrane (cristae) ★ Have their own DNA (mitochondrial DNA or mDNA) important for oxidative metabolism ★ Transform energy for the cell; “powerhouse” of the cell ★ Similar to a battery, generator or power plant ★ Cells have many mitochondria (ex: liver cells have up to 2000!) ★ Each time the cell divides, a mitochondrion divides in two Structure Mitochondria Description Peanut shaped structure with double membrane (outer membrane and highly folded inner membrane called cristae) Function “Powerhouse” of the cell; sites of oxidative metabolism EUKARYOTIC ORGANELLES MITOCHONDRIA http://academic.brooklyn.cuny.edu/biology/bio4fv/page/ mito.gif EUKARYOTIC ORGANELLES THE CHLOROPLAST ★ Greek: chloro = “green”; plasts = “form “ or “entitiy” ★ Commonly in plant cells ★ Contain pigment called chlorophyll (gives plants their green color) ★ Carry out photosynthesis ★ Typically contain one to several hundred ★ Contain two membranes; closed compartment of stacked membranes called grana which have disk-shaped structures called thylakoids ; surrounding thylakoid is a fluid matrix called stroma ★ Other DNA-containing organelles in plants = plastids (leicoplasts; amyloplasts) Structure Chloroplast Description Flat, disk-shaped green organelle with two membranes; contain chlorophyll, a photosynthetic pigment Function Site of photosynthesis; produces food for the cell EUKARYOTIC ORGANELLES CHLOROPLAST http://virtualbiologytutor.co.uk/images/chlo roplast.jpg http://upload.wikimedia.org/wikipedia/commons/4/49/Pl agiomnium_affine_laminazellen.jpeg EUKARYOTIC ORGANELLES THE CENTRIOLES ★ Microtubule Assembly Centers ★ Composed of 9 triplets of microtubules ★ Microtubules = long, hollow cylinders which influence cell shape, move the chromosomes in cell division, and provide structure for flagella and cilia ★ Barrel-shaped organelles found in animals and most protists ★ Plants and fungi lack centrioles ★ Occur in pairs, usually at right angles Structure Centrioles Description Occur in pairs and made of microtubules; in animal cells and most protists Function Important in cell division, cell shape and structure for flagella and cilia EUKARYOTIC ORGANELLES CENTRIOLES http://images.protopage.com/view/721389/3 ydo50yjpdnqy8ag4flqbd1un.jpg http://upload.wikimedia.org/wikipedia/commons/4/49/Pl agiomnium_affine_laminazellen.jpeg EUKARYOTIC ORGANELLES THE CYTOSKELETON ★ Interior framework of the cell ★ Network of protein fibers that support the shape of the cell and anchor organelles to fixed locations ★ Stretches the plasma membrane like the poles on a circus tent! ★ Allows cells to alter rapidly alter shape Structure Cytoskeleton Description Network of protein filaments Function Provides internal structural support; helps in cell movement EUKARYOTIC ORGANELLES CYTOSKELETON http://www.ibri.org/RRs/RR05 1/51cytoskeleton.gif http://www.noble.org/press_release/plantbi o/blancaflornasa/cytoskeleton.jpg http://migration.files.wordpress.com/2007 /07/cytoskeleton02.jpg EUKARYOTIC ORGANELLES FLAGELLA/CILIA ★ Made of microtubules Aid the cell in locomotion or feeding Motion is similar to that of oars in a rowboat Flagella are longer projections that move with a whip-like motion Cilia are shorter, numerous projections that look like hairs. Some protists use a pseudopod (“false foot”) to crawl; similar to squeezing a water balloon at one end forces the balloon to bulge out at the other end. Structure Flagella/Cilia Description Cellular extensions made up of pairs of microtubules Function Aid the cell in locomotion (movement) or feeding EUKARYOTIC ORGANELLES CILIA FLAGELLA http://www.ibri.org/RRs/RR05 1/51cytoskeleton.gif http://img.spar knotes.com/fig ures/2/2faaa24 e75677b6732cd2 4bf35c357da/fla gella.gif http://upload.wikimedia. org/wikipedia/commons /thumb/8/82/Eukaryotic _cilium_diagram_pl.svg/ 120pxEukaryotic_cilium_diagr am_pl.svg.png http://www.bio.miami.edu/~cmalle ry/150/proceuc/c27x7x3flagella.jpg EUKARYOTIC ORGANELLES CENTRAL VACUOLE ★ In Plant cells: Large sac used to store mainly water and other materials (sugars, ions and pigments) Helps to increase the surface-to-volume ratio by applying pressure to cell membrane. In Animal cells: contain many small vacuoles for temporary storage Structure Central Vacuole Description In plant cells, large organelle filled with mainly water, and other materials; takes up most of cell Function Storage center for water and other materials; helps maintain volume of cell EUKARYOTIC ORGANELLES CENTRAL VACUOLE http://www.ibri.org/RRs/RR05 1/51cytoskeleton.gif http://micro.magnet.fsu.edu/cells/plants/imag es/plantvacuolesfigure1.jpg http://i mages. protopa ge.com /view/7 21661/d 2fz30gp lfcumnt whpv6jr 25g.jpg Plant Cell http://biology.unm.edu/ccouncil/Biology_124/Images/centralv acoule.bmp Animal Cell Animal Cell Food vacuoles (fv12, fv13, fv14 and contractile vacuole (cv) http://www5.pbrc.hawaii.edu/alle n/ch19/01h-vor710401-28.html EUKARYOTIC ORGANELLES CELL WALL ★ In Plant and bacterial cells: Composed of fibers made out of cellulose polysaccharide) Cell walls in plants are thicker and stronger than those in bacteria (which are primarily made out of protein polysaccharide) Made up of a primary and secondary wall Also present in fungi and some protists Structure Cell Wall Description In plant cells, outer layer of cellulose; in bacteria, outer layer of protein; also found in fungi and some protists Function Protection and support EUKARYOTIC ORGANELLES CELL WALL http://www.ibri.org/RRs/RR05 1/51cytoskeleton.gif http://library.thinkquest.org/C0045 35/media/cell_wall.gif http://www.learner.org/courses/essential/li fe/images/show2.leaf.jpg EUKARYOTIC ORGANELLES CYTOPLASM / CYTOSOL Clear, gelatinous fluid that fills the interior of the cell excluding the nucleus Contains sugars, amino acids and proteins that the cell uses to carry out its everyday activities Structure Cytoplasm/Cytosol Description Clear, gelatinous fluid that fills the inside of the cell Function Protection and support EUKARYOTIC ORGANELLES CYTOPLASM / CYTOSOL http://www.ibri.org/RRs/RR05 1/51cytoskeleton.gif http://www.cartoonstock.com/lowres/rjo0 896l.jpg http://www2.puc.edu/Faculty/Gilbert_Muth/art0063.jpg EUKARYOTIC ORGANELLES RIBOSOMES Sites of protein synthesis (where the cell produces proteins according to the directions of DNA) Consist of two subunits (small and large) composed of rRNA and protein The individual subunits are synthesized in the nucleolus and then move through the nuclear pores to the cytoplasm, where they assemble. Structure Ribosomes Description Small complex assemblies of proteins and RNA, often bound to endoplasmic reticulum Function Sites of protein synthesis EUKARYOTIC ORGANELLES RIBOSOMES http://www.ibri.org/RRs/RR05 1/51cytoskeleton.gif http://www.estrellamountain.edu/faculty/farabee/biobk/BioBookCELL2.ht ml#Ribosomes (Both images) CELL ANALOGIES FACTORY CELL ANALOGIES FACTORY THE CELL THEORY Classical Theory: Matthius Schleiden and Theodor Schwann (1838) 1. All organisms are composed of one or more cells. 2. The cell is the basic unit of structure and organization of living things. 3. All cells come from pre-existing cells (through cell division). Modern Additions: 4. All cells contain hereditary information which is passed from cell to cell during cell division. 5. All cells are basically the same in chemical composition. 6. All energy flow (metabolism and biochemistry) of life occurs within cells.