Unit 1: Biology “Unity and Diversity” WHAT YOU NEED Nelson Biology VCE Units 1&2 Student Resource and Activity Manual 2014 Biozone (leave in classroom) Nelson Biology Unit 1&2 Practical Guide (leave in classroom) Pens, Pencils, Highlighters, Ruler Exercise Book ASSESSMENT • You will be examined against the following criteria in Unit 1&2; • Knowledge of biological terms and conventions • Understanding of key biological concepts, processes and principles • Application of biological understanding to unfamiliar situations • Evaluation of experimental procedures and results • Analysis of information to solve problems, draw conclusions and/or make predictions ALSO... You MUST attend 85% of classes (10 approved, 3 unapproved) You MUST participate in all practical activities To achieve an “S” ALL aspects of the outcomes must be addressed Topic tests will be conducted throughout the semester It is YOUR responsibility to ensure that deadlines are met ASSESSMENT You will be assessed from A+ to E. If you hand your work in late you will receive an Ungraded mark. You will be assessed through: practical reports, multimedia presentations, oral presentations and posters. You will have a topic test at the conclusion of each chapter and you will have an examination at the end of each unit. ASSESSMENT Your report will contain the following: - SAC 1: practical report average - SAC 2: student designed practical - SAC 3: PowerPoint presentation Test average Review questions CANNOT STRESS THIS ENOUGH! If you are not going to make a deadline for what ever reason come and see me BEFORE the due date. CHAPTER REVIEW QUESTIONS Review Questions are required to be completed for each chapter of your book. The answers for these questions must be well presented and well researched using your books to help you. You will be graded A+ to UG Questions CHAPTER 1 ALL CHAPTER 2 1, 2, 7, 8, 10, 14, 15, 17, 19, 24, 25, 26, 29 CHAPTER 3 1, 3, 4, 5, 8, 9, 12, 14, 15, 16 CHAPTER 4 2, 7, 8, 9, 10, 14, 18, 19, 23, 26 CHAPTER 5 1, 2, 5, 7, 12, 14, 20, 21, 27, 29, 37, 42, 44, 49, 54 NEED HELP? See me if you need help before due dates. If you think you aren’t going to finish on time see me before the due date Having trouble: see me in class, or come to my staffroom either at lunch or during a spare period (staffroom 1) As we near examination times I will run lunchtime revision classes. Ask questions TIPS TO SUCCEED PLAN! Learn glossary terms! Go over work in class! Start homework early! Revise your work – using different methods! See me if you have any problems!!! Your in VCE now!!!!!! TOPIC 1: CELL STRUCTURE & ORGANISATION By the end of this topic, you should know and understand: Cell structure for both prokaryotic and eukaryotic cells. Cell Organisation Cell Functioning – organelle function Biochemical processes – photosynthesis and cellular respiration The role of enzymes 5 kingdoms of life Monera Bacteria (Ecoli) Protista Algae (Amoeba) Fungi Fungi (Mushrooms) Plantae Plants (Gum Tree) Animalia Animals (Humans) Monera Protista Fungi Plantae Animalia TYPES OF ORGANISMS Single celled – made up of one cell Multicellular – made up of many cells Every organism (multicellular) is made up of: 1. Systems – a group of organs that work together to perform a specific function eg: Digestive system, reproductive system, root system. 2. Organs – a collection of tissues which work together to ensure a particular function is performed. eg - Digestive System: Stomach, Liver, small & large Intestines Stomach – made of tissues such as epithelium, smooth muscle cells and blood. 3. Tissues – made up of specialised cells that work together to perform a similar Cardiac function Muscle Tissue Red Blood Cells Nerve Cells Plant Cells Cells. • The basic structural & functional unit of any organism. • Can survive on its own (or has the potential to do so.) • Has a highly organised structure, and has many chemical processes and reactions occurring within it. • Senses and responds to changes in its environment. • Has the potential to reproduce itself. • Differ in shape, size and activities depending on what their role is. Life span of cells. • - The average life spans of some human cells: Stomach cells – 2 days Mature sperm cells – 2 – 3 days Skin cells – 20 – 35 days Red blood cells – about 120 days Why this is possible … but these are generally not replaced during a persons lifetime…. Cell Theory • All living things are composed of one or more cells. • The cell is the smallest form of life. • All cells come from pre-existing cells, via cell division. There are two types of cells: 1. Prokaryotic 2. Eukaryotic. The structure of these cells provides the groupings of all organisms into 5 ‘kingdoms’. Cells of living things (ref: pg 6) Prokaryotic Cells Monera Plantae Eukaryotic Cells Animalia Protista Fungi Common to all cells…. • Each cell is a small compartment with an outer boundary – cell membrane/ plasma membrane - controls entry of dissolved substances into and out of the cell. • Inside each living cell is fluid – cytosol • Cells also all contain genetic material that controls all metabolic activitiesDNA (deoxyribonucleic acid) 1. Prokaryotic Cells • • • • – • • • Organisms known as prokaryotes Simple internal structure. No membrane-bound organelles No membrane-bound nucleus circular DNA Ribosomes Non cellulose cell wall Kingdoms – Monera (bacteria) Generalised Prokaryotic Cell 2. Eukaryotic Cells • Single celled and multi-celled organisms (eukaryotes). • Complex internal structures • Membrane-bound organelles in the cytosol (compartmentalise functions) • Membrane-bound nucleus (nuclear membrane) Kingdoms – Protista - Plantae - Animalia - Fungi A eukaryotic . animal cell • A simple line drawing is the most common way to draw a cell and its organelles. A eukaryotic PLANT cell. Glossary: Your glossary should be in the back of your notebook. Use the glossary in the text, and also the information within the text to write your definition of the following terms: * System * Organs * Tissues * Prokaryotic cells * Eukaryotic cells * Organelles Units of measurement • • • • Metre m = 1m Millimetre mm= 10-3 m Micrometre um = 10-6m Nanometre nm = 10-9m The discovery of cells… • Several key events occurred to construct our understanding of cells: - Galileo Galilei (1609) put glass lenses in a cylinder and found they magnified objects, studying eyes of an insect. - Robert Hooke (1665) used a microscope to observe thinly sliced cork cells. - Anton van Leeuwenhoek (1674) created improved microscopes so magnification was up to 300 times. - Robert Brown (1831) used improve lenses to view plant cells. He noticed the cells contained a nucleus. Microscopes • Light (optical) microscope – Uses light rays to enlarge an image of a specimen through glass lenses – Advantage: can be used to view living cells (provides magnification of up to 400 times), cheap – Disadvantage: limited magnification; stains & dyes need to be used to enhance cell detail but these kill the cell, samples need to be very thin • Electron microscope – Uses an electron beam and electromagnets (instead of glass lenses) – Advantage: extremely clear resolution at a very large magnification (up to 500,000 times) – Disadvantage: specimens must be dead, expensive, images black and white Compound Light Microscopes The ocular lens is 10x magnification. If you use 4x magnification (objective) then the total magnification will be 40x (10 x 4 = 40) Always write the magnification next to your diagram. Dissecting Microscope Laser Scanning Microscope Electron Scanning microscopes • Magnification 80,000 - 500,000x Transmission Electron Microscope Magnification (up to 1500x) The ocular lens is 10x magnification. If you use 4x magnification (objective) then the total magnification will be 40x (10 x 4 = 40) Always write the magnification next to your diagram. Drawing diagrams in Biology • Use Pencil • Never colour-in diagrams (no shading) • All diagrams should use proper titles and labels • Labels should be written horizontally Practical Activity 1.1 Purpose: • to revise and refine microscopic use. • To explore some of the structures of unicellular and multicellular organisms. Cell size & Specialisation • Cell specialisation = cells that have taken on special features to enable them to carry out their task. (eg: nerve cell, red blood cell…) • Size is an important factor in the functioning of cells – the cell’s volume to surface area ratio is crucial. - The cell must be able to efficiently remove wastes and obtain its requirements. The rate of outward movement of wastes and inward movement of requirements will influence the size to which the cell will grow. Cell Size • Cells are measured in: * microns – micrometer (μm = 10-6 of a metre (0.000001m = 0.001mm or 1mm = 1000μm) * nanometres – nm = 10-9 of a metre (or 1μm = 1000nm) Why are cells generally small? • Rheanon’s answer Cells are usually very small because as a cell grows it generally increases more quickly in volume than in surface area, and it will eventually reach a point where the inward movement of requirements and the outward movement of wastes across the surface area are not fast enough to allow the cell to grow any more and still function efficiently. Surface – Area – To – Volume Ratio • The SA:V ratio of any object is obtained by dividing its area by its volume. • Area refers to the coverage of a surface - cm² • Volume refers to the amount of space taken up by an object - cm³ • The SA:V ratio of a shape identifies how many units of external surface area are available to ‘supply’ each unit of internal volume. • In general, as a particular shape increases in size, the SA:V ratio of the shape decreases. • Cells with outfoldings can exchange matter with their surroundings more rapidly than cells lacking this feature. ‘Taking on different jobs’ • • • • • • • • • Boundary – plasma membrane and cell wall Power Supply – mitochondria Building Cell Structures – ribosomes Supporting Cell Structures – cytoskeleton Transport with the Cell – endoplasmic reticulum Packaging & Distribution – golgi apparatus Recycling & Reuse – lysosomes Moving in & out – endocytosis & exocytosis Coordinating cell activities – nucleus Coordinating cell activities - Nucleus • Control centre of eukaryotes. • Coordinates all actions within the cell • Main physical feature of a eukaryotic cell – usually seen as a dark organelle. • Separated from the rest of cell by nuclear membrane/envelope (double membrane). • Contains DNA (Codes for the production of proteins that carry out different functions within a cell), and the nucleolus • Mature RBC – no nuclei Nucleolus • Made of protein and ribosomal RNA (ribonucleic acid) • Manufactures proteins in the cell. ‘Taking on different jobs’ 1a. Boundary - Plasma membrane • Outermost barrier in animal cells • Found in all living cells (prokaryotes and eukaryotes) • Seen using an electron microscope • Made of lipid (fat) molecules with tiny protein channels passing through it to allow movement of molecules (nutrients & wastes) in and out of cell. 1b. Boundary - Cell Wall • Outermost barrier in plant, fungi, bacterial and most algae cells. (not present in animal cells) • Provides extra support, shape & protection. (some larger plants have a double cell wall – ie. Cells in tree trunk) • Cell wall is made from: * Plants = cellulose * Fungi = chitin * Bacteria = proteins & polysaccharides (peptidoglycan) • When a plant cell’s contents die, it leaves a hollow tube where nutrients and water can flow. Cytoplasm • The fluid, dissolved substances and organelles within the cell between the plasma membrane & the nuclear membrane, where all the activities are carried out. • The fluid is called cytosol. 2. Power house- Mitochondria • Only in eukaryotes, seen with an electron microscope • Site of cellular respiration (aerobic respiration) releasing energy for the cell (form of ATP). Cellular respiration equation: • Glucose + Oxygen • C6H12O6 + 6O2 Carbon dioxide + water + heat energy 6C02 + 6H2O The inner membranes (cristae): * folded to provide a large surface area for the reaction to occur. * contain an enzyme that catalyses the reaction. Energy production • The energy released by mitochondria is called ATP (adenosine triphosphate) • ATP (chemical energy) powers all cell processes and therefore keeps us alive • Where would mitochondria be highly prevalent? 3. Building cell structures - Ribosomes • Present in prokaryotes and eukaryotes (not membrane bound) • Very small organelles composed of protein and RNA (ribonucleic acid) • Manufacture proteins from amino acids – helps cells grow, repair damage etc • Scattered throughout the cells cytoplasm OR • Can be attached to endoplasmic reticulum • Seen using an electron microscope 4. Supporting cell structure - Cytoskeleton • internal framework in cytoplasm- shape and structure Microtubules • Hollow cylindrical tubes, scaffold • ‘rails’ for organelles to move on. • Constant mixing and movement of the cytoplasm = cytoplasmic streaming • Can come apart and Reassemble. Microfilaments – *Solid & contractile *allow the cell to change shape. (eg: muscle cells) • Intermediate filaments provide tensile strength for attachment of cells to each other to support long nerve cell extensions and maintain tissue shape. Only in animal cells - Centrioles • Replicate before division to produce two pairs • Give rise to spindle fibres which chromosomes attach to • When spindle fibres contract, chromosomes are moved around the cell 5. Transport within the cell - Endoplasmic Reticulum • Only present in eukaryotes • An Intracellular (inside cell) transport system. • A system of membranous channels, allows substances to move through the cell. • Small sacs (vesicles) can be pinched off, allowing molecules to be transported around the cell to other organelles * Two types – rough & smooth. (i) Rough Endoplasmic Reticulum. • Ribosomes are stuck to the ER making it look rough. • Proteins produced can move directly into ER and move around the cell. • Membrane factory • Excretory proteins – hormones, enzymes – move to other cells (ii) Smooth Endoplasmic Reticulum • Has no ribosomes attached to its membranes. • Syntheises fats, phospholipids, steroids… • transports proteins – vesicles ‘break off’ ends. 6. Packing & Distribution - Golgi • Only in eukaryotes • Works closely with smooth E.R. • ‘Packages’ and stores molecules (proteins, such as digestive enzymes) for their release. • Consists of a system of stacks of membranes. The ends ‘pinch off’ into vesicles, which can then move to the plasma membrane and fuse for release outside the cell. Apparatus 7. Recycling & reuse – Lysosomes (AKA – ‘suicide sacs’) • Only found in eukaryotes • Formed by the Golgi Apparatus. • Contains digestive enzymes which split large chemical compounds into simpler usable molecules. • Membrane breaks, enzymes released to destroy the cell by digesting the contents. • ‘Apoptosis’ – programmed cell death when the cells are old or no longer needed • Syndactyly – results if apoptosis doesn’t occur. 8a. Moving in – Endocytosis & 8b. Moving out - Exocytosis. • Molecules must be able to move into the cell (nutrients) and out of the cell (proteins/wastes). • Exocytosis – a small membrane-bound vesicle joins to the plasma membrane, and releases its contents to the outside of the cell. • Endocytosis – the plasma membrane sinks and forms a vesicle enclosing the material bringing it into the cell. • When the material is a solid food = phagocytosis. When the material is in solution = pinocytosis. Transportation within a cell. Specialised structures in Plants. 1. Adding colour - Plastids • A group of organelles containing colour pigments. Chromoplasts • carotenoid pigments (red-yellow) • turn green as they mature (produce chlorophyll) • found in flowers and leaves. Leucoplasts • colourless. • amyplasts - store starch grains. • found in roots (also root vegetables) Chloroplasts • • • • only found in plant and algae cells contain the green chlorophyll pigment. absorbs light energy for use in photosynthesis. Grana – folded membrane layers (lamellae) – provide large surface area where chlorophylls are located. • Stroma – fluid between the grana. • Photosynthesis occurs in the stroma and thylakoid membrane system. Chlorophyll • Green part of plants (plastid). • internal membranes are folded - maximise surface area. • Absorbs sunlight • Photosynthesises – the chemical reaction using sun energy to convert carbon dioxide and water into glucose and oxygen. Photosynthesis reaction: • • Carbon dioxide + water 6CO2 + 12H2O Glucose + oxygen + water C6H12O6 + 6O2 + 6H2O 2. Moving things about – Xylem & Phloem. • move water and nutrients around a plant in vascular tissue • series of hollow tubes. • give strength to plant stems and tree trunks. Xylem • • • • • Water & minerals Roots to leaves (UP) Tracheids and vessels Dead & hollow cells Strengthened with lignin rings Phloem • sugars in solution (from photosynthesis) • sieve & companion cells • Sugar flows through sieve cells/tubes • Companion cells control sieve cells Vascular tissue 3. Storage facility - Vacuoles • Membrane bound fluid filled spaces • Storage facility for various substances – mainly water and nutrients • expand - up to 90% of the cell’s volume. • cell wall prevents the plant cell from exploding. • Generally large in plants • In animals - food vacuoles are involved with intercellular digestion. Moving from place to place - Active Movement. Paramecium • Cilia – hair like structures that propel the cell forward. However, they are often found lining ducts, along which materials can be moved up or down by means of their rapid and rhythmical beating. (eg: lungs) • Flagellum – a long whip-like tail that pushes cell forward. Attached to cell membrane. (eg: sperm) • Corkscrew movement (prokaryote) • Wavy movement (eukaryote) • Both contain microtubules – Passive Movement. Moving from place to place • cells move by floating in something such as water or plasma (eg: red blood cells) Glossary Terms: Add these terms to your glossary. * Cellular Respiration * Vesicle * Apoptosis * Enzymes * Spindle Fibres * Intercellular * Intracellular * Stroma * Thylakoid * Lignin * Cytoplasmic Streaming Last Slide