Christine Ann Lynch clynch13@gmail.com, cell 865-385-2444 2823 Winam Ave Apt I1, Honolulu, HI 96816 EDUCATION PhD in Entomology, Washington State University Pullman, WA 99164 GPA 3.64/4.0 January 2010- May 4, 2013 Masters of Science in Entomology, University of Tennessee Knoxville, TN 37996 GPA 4.0/4.0 December 17, 2006 Bachelors of Science in Entomology/Nematology, University of Florida Gainesville, FL 32611 GPA 3.25/4.0 June 19, 2004 Saint Thomas Aquinas High School Fort Lauderdale, FL 33312 May 2000 ADDITIONAL EDUCATION Completed distance learning class ENY 6203 and ENY 6203L Insect Ecology & lab University of Florida Gainesville, FL 32611 GPA 4.0/4.0 Grant writing workshop Washington State University October 27, 2012 WORK EXPERIENCE Junior Researcher (Postdoctoral researcher), University of Hawai‘i Honolulu, HI August 1, 2013 to present Collaborated with USDA APHIS PPQ CPHST to change current export restrictions and regulations by determining if a specialty crop like moringa pods was a fruit fly host Contacted growers on small farms to set up meetings and moringa sampling schedule Set up field experiment to monitor moringa tree growth and insect pests weekly by removing all the pods from 88 moringa trees and trimming 40 of these trees for the study Collected moringa pods monthly from five commercial growers and two UH research stations Brought moringa pods from farms back to laboratory, set up cages with pods inside and observed insect emergence Identified insect specimens that emerged from moringa pods held in the laboratory Collected rotting fruit for establishment of fruit fly colonies for Bactrocera dorsalis (oriental fruit fly) and B. curcurbitae (melon fruit fly) and set up breeding colonies Helped mentor three undergraduate students who were conducting independent research projects in the laboratory and greenhouse Wrote an extension publication, quarterly updates for USDA APHIS, and grants for future research Graduate Research Assistant, Washington State University Pullman, WA January 2010-May 2013 Set up field experiment by measuring, staking out plots and helping to plant potatoes Manipulated Myzus persicae (green peach aphids) and Leptinotarsa decemlineata (Colorado potato beetles) by spraying pesticides in specific plots and spraying water in control plots to determine if generalist predators would be more attracted to a one or both target prey species Sampled insect and spider community on experimental potato field using D-vac suction sampler Manipulated green peach aphids densities and predator combinations in greenhouse and large scale field cage experiments Reared green peach aphids on potatoes in greenhouse and 8 m3 field cages Reared Acyrthosiphon pisum (Harris) (pea aphids), Frankliniella occidentalis (Pergande) (western flower thrips), and Tetranychus urticae Koch (two-spotted spider mites) for feeding trials Ran feeding trials with Geocoris bullatus (Say) (Large Big-eyed bug) and Nabis alternatus Parshley (Western damsel bug) to determine how long western flower thrip DNA is detectable at 0, 2, 4, 6, 8, 16, and 24 hours after consumption Grew potatoes from seed and transplanted young plants in greenhouse Released green peach aphids into experimental field plots Counted insects present on ten plants in each field plot and recorded data Identified insect specimens from field survey and experiment samples Conducted commercial potato field surveys in 32 total fields over two field seasons where soil samples, leaf samples, insect community, and generalist predators for molecular gut content analysis were collected Sampled commercial potato fields for two-spotted spider mites and processed samples to determine if twospotted spider mites were present Investigated Geocoris bullatus (Say) and Nabis alternatus Parshley behavior and consumption of green peach aphids at different densities and predator diversity (single or both species) in greenhouse experiments Examined predator location and consumption when green peach aphids were clumped or dispersed in large scale field cage experiment (ninety 8 m3 cages) Entered data from field experiment and commercial field surveys and kept a lab notebook with experimental data and procedures Used farm maps to navigate and find correct commercial potato fields Trained and supervised 8 undergraduate student employees over 3 summer field seasons Supervised and trained 3 student employees in sorting insect samples from commercial potato fields Interviewed students for one of the 2011 summer positions and for all 3 fall 2011 positions Biological Science Technician (Insects), USDA Agricultural Research Service, U. S. Horticultural Research Laboratory: Subtropical Insects Research Unit Fort Pierce, Florida December 26, 2007- December 22, 2009 April 1, 2009- December 22, 2009 (GS-0404, 07, Step 3-4) Started and maintained Phyllocnistis citrella Stainton (citrus leafminer) colonies from field collected specimens Collected new flush branch samples and counted the incidence of canker, leaf mines, and canker in leaf mines on leaves Set up and ran field experiment to determine if canker was found more frequently in citrus leafminer trails or on the rest of the leaf Entered data from branch sample collection and kept a lab notebook with experimental data and procedures Dissected Diaphorina citri Kuwayama (Asian citrus psyllids) and collected psyllid organs such as midgut and salivary glands that were tested for presence or absence of HLB using qPCR Put out SPLAT CLM for citrus leafminer mating disruption and citrus leafminer traps in commercial citrus grove trial December 26, 2007- March 31, 2009 (GS-0404, 07, Step 2, 3) Maintained and reared Diaphorina citri Kuwayama (Asian citrus psyllid), Toxoptera citricida (Kirkaldy) (brown citrus aphids), and brown citrus aphid parasitoid colonies Setup and completed experiment on the effects of PFR-97 fungus on a parasitoid of brown citrus aphids Extracted RNA and DNA from Asian citrus psyllids Performed PCR, gel electrophoresis, and DNA cleaning of samples after extraction of DNA 2 Dissected over 400 Asian citrus psyllids and collected psyllid organs such as midgut, ovaries, testes, mycetocytes, and fat bodies for later use in different cDNA libraries Completed Asian citrus psyllid midgut cDNA library WHDcMg and testes cDNA library WHDcT using the Zap-cDNA Synthesis Protocol from Stratagene Wrote protocol for creating cDNA libraries that included dissections of Asian citrus psyllids for tissue or organs and made modifications to RNA extraction, DNase treatment, RNA clean up kit, and zap library protocol Edited the sequences for midgut and testes libraries in Sequencher 4.8 and sent EST sequences to NCBI for posting Removed vector, assembled sequence data, and created FASTA files using Sequencher 4.8 to use for blastx and blastn searches on the NBCI website Used digital photography to take pictures of different psyllid eye colors and stained or unstained tissues/organs from psyllid dissections Illustrated psyllid anatomy from digital images for use in presentations and papers Dissected, stained, and photographed psyllid salivary glands under microscopes Established inbred psyllid colonies by collecting the correct stage of psyllid nymphs, pairing emerged adults for inbreeding, maintaining colonies, continuing to further inbreed pairs, and maintaining records of eggs laid, nymph and adult emergence times, and other pertinent information Processed hundreds of DNA/RNA samples, maintained sample identity, and kept experimental data on computer and in notes under tight deadlines for the Multivirus project on bee viruses Coordinated field experiment and sampling with the University of Florida, Beeologics personnel and Florida Department of Agriculture and Consumer Services beekeepers for Israeli Acute Paralysis Virus field trial to determine if the RNAi product called Remebee would prevent colony collapse disorder Fed honey bee hives sucrose for controls and added IAPV, Remebee, or IAPV and Remebee to sucrose in mason jars for field trial Helped work honey bee hives to sample live bees, count hive forager activity, and determine hive health on each frame for 100 hives over 6 weeks Prepared materials for the IAPV field experiments and recorded notes on each session Maintained accurate experimental data on computer and lab notebooks for Multivirus project Extraction of RNA, DNase treatments, SuperScript III First Strand Synthesis system for RT-PCR, PCR and gels, process samples for Multivirus project Trained one summer volunteer how to use the Nano Drop ND-1000 Spectrophotometer for quantification of nucleic acids, pick cDNA library colonies for sequencing, water insect colonies down in the insectary and the lab, and how to use the autoclave Instructed two summer volunteers on RNA extraction, RNeasy kit use, and Multivirus samples Provided instruction to post-doctoral researcher on how to import, trim and align sequences in Sequencher 4.8 Created a poster for demonstration, helped set up poster and table area, greeted the public and answered questions about demonstration for ARS Open House 2008 Informed the public about importance of HLB to citrus production, the role of Asian citrus psyllids as vectors and current research done by different technicians in the lab Helped collect psyllids from citrus trees in the field for various research projects Provided psyllids from my colony for virus testing and different organs from dissected psyllids for postdoctoral research projects Recorded and developed protocols, conducted experiments, kept accurate and neat lab notebooks, plus daily watering and lab maintenance Produced new flush by pruning Carrizo and Mexican lime plants periodically to ensure insect numbers for experimental needs 3 PPQ Technician (Surveyor), USDA APHIS PPQ CHRP Melbourne, FL October 30, 2006 - December 22, 2007 (GS-0421, 07) Identified citrus canker, Huanglongbing (HLB), and other target pest suspects in commercial citrus groves Used maps and aerial photos to determine which grove blocks were to be surveyed Documented inspection of each grove and any suspect samples Updated text submissions from contributors, revised assigned sections, and edited the layout of the CHRP standard operating procedure for interim and proposed citrus quarantine rules Visited package shippers to inform them of citrus shipping regulations Inspected citrus fruit in commercial packing houses for citrus canker and followed the proper protocol when a suspect was found Sealed containers containing quarantine citrus for transport to Canada or for export Completed paperwork for daily reports and truck manifests Entered shipping manifest information into CHRP database Maintained professional relationship with packing house management and informed them about regulatory procedures Entomology Research Assistant, University of Florida’s Fort Lauderdale Research & Education Center Fort Lauderdale, Florida September 2006 – October 2006 Extracted DNA from Homaledra sp. (Lepidoptera) specimens on palms from Florida and Louisiana using Dneasy kit Performed PCR, gel electrophoresis, and DNA cleaning of samples after extraction of DNA Created procedural handout that combined all of the protocols for processing DNA samples Located, mapped, numbered and took GPS coordinates for field colonies of Pheidole megacephala (Fabricius) (Big-headed ants) Set up pesticide assay using field colonies of Big-headed ants Counted the number of ants in each sample for past pesticide studies Prepared food and fed the field colonies of Big-headed ants Helped capture mega colony of Big-headed ants and set up laboratory colony Graduate Research Assistant, University of Tennessee Knoxville, TN August 2004 – August 2006 Recorded predators of Fiorinia externa Ferris (elongate hemlock scale) in the southern Appalachians Identified and assessed Coccinellidae, Mycetophagidae, and Coniopterygidae predators in forest and urban sites Evaluated behavioral feeding responses, consumption, and intraguild competition of six predators feeding on elongate hemlock scale in laboratory tests Collected predators from Tsuga canadensis (L.) Carrière (eastern hemlock) throughout the year using a beat sheet Assessed predator field damage on adult female scales from eastern hemlock branch samples counted in the laboratory Presented research data at scientific meetings Recorded GPS readings for precise locations of each block and site Used maps to navigate and separate blocks at sites Prepared data results for grant update papers Searched for scientific papers for use in thesis and presentations Documented sites photographically Photographed specimens under the microscope 4 Career Ambassador UF Career Research Center Gainesville, FL January 2003- May 2004 Advised students on careers, resumes, and cover letters Gave outreach presentations about career topics to classes and organizations Conducted mock interviews to assist students with interviewing skills Entomology/Nematology Research Assistant, University of Florida’s Fort Lauderdale Research & Education Center Fort Lauderdale, Florida June 2003 - August 2003 Maintained captive colonies of Technomyrmex difficilis Forel (white-footed ants) & carpenter ants for future experiments Prepared and replaced vials of sugar water and insecticide in boxes on palm trees for testing of a new possible control of Technomyrmex difficilis Forel Collected new white-footed ant colonies from palm tree crowns Cleaned termite samples from Trinidad and Tobago May 2002 – August 2002 Cultured nematodes for collecting & DNA testing Monitored population of nematodes for stages & growth patterns Collected Oxyops vitiosa (Pascoe) (melaleuca weevil) off melaleuca to assist with augmentation of this biocontrol agent for USDA project Checked & maintained specimens in international termite collection Prepared food for white-footed ants study Organized and prepared specimens for taxonomically arranged Cornell drawers December 2001 – January 2002 Optimized photos and clarified drawings for nematode database May 2001 – August 2001 Collected & raised Rhynchophorus cruentatus Fabricius (palmetto weevil) Helped conduct experiment testing pesticide efficiency on palm weevil larvae Entered data on effectiveness of different concentrations of pesticides Worked on nematode database by scanning & optimizing slides and drawings February 2000 – August 2000 Set up & conducted nematode extractions, prepared culture media Recorded data, scanned transmission electron micrographs & slides Entered data into computer, updated chemical inventory POSTER PRESENTATIONS Xie, Y., P. Lambdin, J. Grant, and C. Lynch. 2008. Behavior and consumption of Scymnillus horni (Coleoptera: Coccinellidae) on the elongate hemlock scale, Fiorinia externa (Hemiptera: Diaspididae), pp. 205-211. in: Proc. XI International Symposium on Scale Insect Studies. Lynch, C., P. Lambdin, J. Grant, R. Reardon, & R. Rhea. 2006. Predators of Fiorinia externa Ferris (Hemiptera: Diaspididae) on eastern hemlock, Tsuga canadensis (L.) Carriere, in urban and forest sites. National Entomological Society of America meeting. Indianapolis, IN (Dec. 10-13). 5 Lynch, C., P. Lambdin, J. Grant, R. Reardon, & R. Rhea. 2006. Predators of Fiorinia externa (Hemiptera: Diaspididae) on eastern hemlock in southern Appalachians. North American Forest Insect Work Conference. Asheville, NC (May 22-25). Lambdin, P., C. Lynch, R. Reardon, B. Onken, R. Rhea & J Grant. 2005. EHS and its natural enemies in the southern Appalachians. 3rd Symp. HWA in Eastern U. S., Asheville, NC (Feb. 1-3). Lynch, C., P. Lambdin, P. McNicholas & J. Grant. 2005. Survey for parasitoids and predators of the elongate hemlock scale in the southern Appalachians. Southeastern Branch meeting of Entomological Society of America, Tunica, MS (Mar. 6-9). Lambdin, P., P. McNicholas, C. Lynch & J. Grant. 2005. Density and distribution of the elongate hemlock scale in the southern Appalachians. Southeastern Branch meeting of Entomological Society of America, Tunica, MS (Mar. 6-9). Lynch, C., P. Lambdin, J. Grant, R. Reardon, & R. Rhea. 2005. Predators of Fiorinia externa (Hemiptera: Diaspididae) on eastern hemlock in urban and forest sites. National Entomological Society of America meeting. Fort Lauderdale, FL (Dec. 15-18). ORAL PRESENTATIONS Lynch, C., H. Spafford, and N. Liquido. 2014. Commodity export regulation and emerging niche markets: moringa tree, Moringa oleifera Lam., and fruit fly species (Family Tephritidae) in Hawai‘i. Pacific Branch meeting of the Entomological Society of America, Tucson, AZ (April 6-9). Lynch, C. A., W. E. Snyder, E. G. Chapman & J. D. Harwood. 2012. Manipulation of Myzus persicae, green peach aphid, and Leptinotarsa decemlineata, Colorado potato beetle, to determine predator distribution in potatoes. National Entomological Society of America meeting, Knoxville, TN (November 11-15). Lynch, C. A., W. E. Snyder, E. G. Chapman & J. D. Harwood. 2012. Manipulation of Myzus persicae, green peach aphid, and Leptinotarsa decemlineata, Colorado potato beetle, to determine predator distribution in potatoes. Pacific Branch meeting of the Entomological Society of America, Portland, OR (March 25-28). Lynch, C. A., W. E. Snyder, E. G. Chapman & J. D. Harwood. 2011. Manipulation of Myzus persicae, green peach aphid, and Leptinotarsa decemlineata, Colorado potato beetle, in potatoes to determine predator food preference. National Entomological Society of America meeting, Reno, NV (November 13-16). Lynch, C., P. Lambdin, J. Grant, R. Reardon, & R. Rhea. 2006. Assessment of established predators on elongate hemlock scale populations. Southeastern Branch meeting of the Entomological Society of America, Wilmington, NC (Mar. 5-8). Lynch, C., P. Lambdin, J. Grant, R. Reardon, & R. Rhea. 2006. Asessment established predators on elongate hemlock scale populations. SAFEPS, Crossnore, NC (Mar. 9-10). Lynch, C., P. Lambdin, J. Grant, R. Reardon, B. Onken & R. Rhea. 2005. Natural enemies of the elongate hemlock scale in the southern Appalachians. SAFEPS, Crossnore, NC (Feb. 17-18). 6 Lynch, C., P. Lambdin, J. Grant, and R. Rhea. 2005. Predators of Fiorinia externa (Hemiptera: Diaspididae) on eastern hemlock in urban & forest sites. TES meeting, Nashville, TN (Oct 13-14) PUBLISHED PAPERS Lynch, C. and H. Spafford. Determining fruit fly (Family Tephritidae) host status of moringa tree pods, Moringa oleifera Lam., in Hawai'i. Hānai‘Ai / The Food Provider. Volume 20: June/July/August 2014. http://www.ctahr.hawaii.edu/SustainAg/news/articles/V20-Lynch-Spafford-FruitFlyMoringa.pdf Ammar, D., R. G. Shatters, Jr., C. Lynch, and D. G. Hall. 2011. Detection and relative titer of Candidatus Liberibacter asiaticus in the salivary glands and alimentary canal of its vector Diaphorina citri (Hemiptera: Psyllidae) vector of citrus Huanglongbing disease. Annals of Entomological Society of America. 104(3): 526-533. Xie, Y., P. Lambdin, J. Grant, and C. Lynch. 2008. Behavior and consumption of Scymnillus horni (Coleoptera: Coccinellidae) on the elongate hemlock scale, Fiorinia externa (Hemiptera: Diaspididae), pp. 205-211. in: Proc. XI International Symposium on Scale Insect Studies. Lynch, C., P. Lambdin, J. Grant, R. Reardon, and R. Rhea. December 2006. Survey for potential predators of the elongate hemlock scale in Tennessee and North Carolina. Florida Entomol. 89 (4): 527-528. BOOK CHAPTER Lynch, C. A, D. W. Crowder, R. Jabbour, and W. E. Snyder. 2013. Spud web: Species interactions and biodiversity in potatoes, pp. 271-290. In P. Giordanengo and A. Alyokhin (eds.), Insect Pests of Potatoes: Global perspectives on biology and management. Academic Press (Elsevier) Oxford, UK. , Waltham, MA. TEACHING EXPERIENCE USDA APHIS Ag Discovery Program University of Hawai‘i July 21, 2014 Taught ten high school students with an interest in agriculture about the field of entomology, ecological role of insects, agricultural insect pests, careers in entomology and USDA, how to use a dissecting microscope and fruit fly management strategies Prepared lecture materials and directions for microscope use along Set up insect display Cornell drawers and live walkingsticks, cockroaches, monarch caterpillar, and fruit flies Showed slides with different arthropods and insects and had students guess which were insects after teaching them the distinction between insects and other arthropods Demonstrated two different types of fruit fly traps through using McPhail traps for collecting female fruit flies and bucket traps with methyl eugenol male pheromone at two outside locations on UH campus Created dichotomous key that separated the four fruit fly species in Hawai‘i by wing patterns that each student used to identify a live fruit fly to species Answered questions about the lecture material and other general questions about insects ENTOM 340 Agricultural Entomology Laboratory section Washington State University Spring 2011 Set up lab materials, assisted with lab, answered students’ questions, demonstrated cockroach dissection, and graded handouts Taught laboratory lecture on insects used in biological control 7 ENTOM 150 Insects, Science, and World Cultures Washington State University Fall 2010, 2011, & 2012 Assisted with aquatic insects field trip to Asotin creek in (Fall 2010 and 2011) Assisted with forensic entomology field trip to dead animal pit (Fall 2011) Taught two week section on biological control that included 2 lectures and 2 labs (Fall 2011) Created biocontrol experiment and writing assignment for lab using ladybeetles and aphids (Fall 2011) Helped judge final group projects (experiment with milkweed bugs, Fall 2011) Assisted with compost and organic farm field trip on WSU campus (Fall 2012) INFORMAL INSTRUCTION WSU potato field days (Warden, WA) 2010 and 2011 Gave one 10 minute talk to other researchers and growers in 2010 and two 10 minute talks in 2011 Other instruction Trained researchers at WSU and OSU in proper Dvac suction sampler use for collecting insects on potatoes Supervised and trained 8 student summer employees in greenhouse and field research techniques at WSU Supervised and trained 3 student employees in sorting insect samples from commercial potato fields Trained and supervised 2 interns at USDA ARS in laboratory techniques for RNA extraction PROFESSIONAL ASSOCIATIONS Entomological Society of America, member 2003-present Hawaiian Entomological Society, member 2013-present Florida Entomological Society, member 2005-2009 Tennessee Entomological Society, member 2005-2006 HONOR SOCIETY Gamma Sigma Delta (The Honor Society of Agriculture) inducted November 8, 2005 LEADERSHIP Treasurer of WSU Entomology Graduate Student Association 2010-2012 Treasurer of UT Entomology/Plant Pathology Graduate Student Association 2004-2006 Treasurer of Women’s University of Florida Roller Hockey & Gator Outdoor Clubs 2001-2004 AWARDS & HONORS Awarded George Tamaki Memorial Scholarship from WSU Entomology Department for travel to ESA National Meeting in November 2012 Awarded GPSA registration grant for oral presentation at ESA National Meeting in November 2012 Awarded Hallgarth Scholarship from WSU Entomology Department for travel to ESA National Meeting November 2011 Awarded GPSA travel money for travel and registration for oral presentation at ESA National Meeting November 2011 Graduate Research assistantship at Washington State University ($19,000 annually) Allen-Abrahamson Award, Second place in Masters poster competition at the North American Forest 8 Work Conference Student Competition Southeastern Branch of the Entomological Society of America's 2006 photomicroscopy photo salon winner Graduate Research assistantship at the University of Tennessee ($13,000 annually) Awarded travel money from GSA & CASNR for trip and oral presentation to Southeastern Branch of meeting in March 2006 Milledge Murphey, Sr. Scholarship for scholastic achievements in Entomology Selected to Serve as a University of Florida Career Ambassador Bright Futures Florida Academic Scholars Award (100%) COMPUTER SKILLS SAS 9.2 and 9.3, JMP 10, and Sigmaplot Adobe PageMaker 6.0, Illustrator 6.0, PhotoShop 5.0, Quark, Bryce 3-D 4.0 Corel Wordperfect, Microsoft Office 2003, 2007 & 2010; Windows 95, 98, 2000, XP, Vista, 7 Sequencher 4.7 and 4.8 CERTIFICATIONS First Aid CPR/AED for adults VOLUNTEER SERVICE Entomological Society of American Pacific Branch meeting Tucson, AZ April 8, 2013 Co-moderated General Paper II session Kept talks running on time, introduced speakers, and handled equipment problems University of Hawai‘i Ka Mea Kolo outreach UH Manoa Children’s Center November 22, 2013 Assisted with presentation about insects to preschool age children Answered questions about insect displays and live insects WSU Cult Classic March 26, 2010 Helped expand visitors’ knowledge of insects through hands-on exhibits including live insects Insect Expo March 27, 2010 and April 8 2012 Helped expand visitors’ knowledge of insects through hands-on exhibits including live insects Assisted with cockroach races Painted insects on children’s faces Spoil Island Project Indian River Lagoon work group April 14, 2007 to May 2009 Assisted with the removal of the invasive plants (Brazilian pepper and Australian pine) on recreational spoil islands in Indian River lagoon in Brevard and Indian River Counties Planted mangroves, sea grapes, and other native vegetation on spoil island IR-36 and others University of Tennessee Entomology/Plant Pathology outreach Ag Day September 1, 2005, Blooms Day June 26, 2005, & CASNR Open House April 2005 & 2006 Helped expand visitors knowledge of insects through hands-on exhibits including live insects Ijams River Rescue April 2, 2005 Picked up and disposed of trash from the banks of the Tennessee River University of Florida Marine Collection Department Fall 2002 Maintained marine teaching lab tanks, did collections & curations UF Entomology Nematology Student Organization outreach Morningside Nature Center Spring 2002 Expanded visitors knowledge of insects through hands-on exhibits including live insects 9 University of Florida Museum of Natural History Fall 2001 Assisted in excavation of Newberry Rhino Dig Museum of Discovery & Science, 1994-1999 800 Volunteer hours, educational programs & outreach LANGUAGE SKILLS Spanish: Novice reading, writing, and speaking OUTDOOR SKILLS Driving trucks over tough terrain Hiking, backpacking, rock climbing, sea kayaking, and whitewater kayaking Snorkeling, Scuba open water and Advanced certifications Cross country skiing, downhill skiing, and snowshoeing 10