BCH 3033
General Biochemistry Flipped
Welcome to the Fall-2013
Inverted or Flipped General Biochemistry Course
The “Lectures” are Online
You Need to Gain Biochemical Knowledge BEFORE Class
Class Time = Quizzes + Problem Solving + Case Studies
CLASS TIME IS TO EXERCIZE
YOUR KNOWLEDGE
© 2013 W. H. Freeman and Company
Syllabus on Blackboard Learn
And at… http://www.fiu.edu/~bch3033
Advantages of Blackboard Learn:
1. The “Lectures” = Narrated Power Points
2. Case studies as pdf files.
3. You can create your Discussion Groups
4. Grade Book…keeps updating your grades
with each class…Clicker Scores and
Exams..includes class statistics
You Need to get an iClicker…the graded part of class
time begins next week.
What’s Involved ?
LEARNING BIOCHEM
The Standard 3 Credit Course generally needs 6-8 hours SERIOUS
study/week
You will need to LEARN the “Lectures” and Solve End of Chapter
Problems BEFORE Class Time
You will see that some “Lectures” are short, ~35 minutes, others are
about an hour. BEFORE CLASS you will need to practice what you
learned from the “lecture” by doing end of chapter problems.
Study ? Gain Knowledge ?
What is the “trick”?
DO IT BEFORE CLASS !!
BEFORE CLASS LEARNING to develop Deep Knowledge….
not shallow knowledge !
Study ? Gain Knowledge ?
Class
a
What is the Deal? Each Flipped Class requires Mindful Study.
Flipped Classes: EXERCISE Your Knowledge IN CLASS
This Inverted or Flipped Class
All the “Lectures” are on-line narrated PowerPoints…..
You need to LEARN the PowerPoints and DO Selected
End of Chapter Problems BEFORE CLASS
Class time will be used doing “advanced homework”:
problem solving using chemistry, math, and biochemical
concepts.
Every Class has a Graded Component = 1/3 of Total Grade,
Class Grade comes from iClickers
The Syllabus is on Blackboard Learn
Answers and Short Solutions to EOC Problems start
after page 1198 in the text.
Class Response System: iClickers
iClickers come in different forms
You can buy new or used iClickers
from the bookstore or used
ones from friends, older
models will work fine as long
as the batteries are working.
or get an App for your Smart
Phone, Tablet or Laptop
iClicker Registration
You need to REGISTER your iClicker: go to iClicker.com
You need:
Your name,
PantherID and the
iClicker Serial Number (on the back of
the iClicker)
If you get a used iClicker, make sure the Serial Number
is clearly visible and not rubbed off.
General Biochemistry has 3 Parts
PART 1: Chemical Structure of: Proteins (amino
acids), Polysaccharides (sugars), Nucleic Acids
(nucleotides) and Lipids (glycerol and fatty acids,
steroids, ceramides)…and chemical methods of their
purification and their structure determination.
PART 2: Mechanisms: Ionic dissociation, pH, enzyme
kinetics, thermodynamics, signaling mechanisms. This
part integrates into Parts 1 and 3.
PART 3: Metabolic Pathways and Their Control. We will
focus on Central Metabolism: Glycolysis,
Gluconeogenesis, Pentose Phosphate Pathway, Citric
Acid Cycle, Fatty Acid and Amino Acid catabolism and
Electron Transport.
General Biochemistry has 3 Parts
PART 1: Chemical Structure of: Proteins (amino
acids), Polysaccharides (sugars), Nucleic Acids
(nucleotides) and Lipids (glycerol and fatty acids,
steroids, ceramides)…and chemical methods of their
purification and their structure determination.
PART 2: Mechanisms: Ionic dissociation, pH, enzyme
kinetics, thermodynamics, signaling mechanisms. This
part integrates into Parts 1 and 3.
PART 3: Metabolic Pathways and Their Control. We will
focus on Central Metabolism: Glycolysis,
Gluconeogenesis, Pentose Phosphate Pathway, Citric
Acid Cycle, Fatty Acid and Amino Acid catabolism and
Electron Transport.
Biochemistry Celebrates Biological Diversity
What about the Microbes ?
Biochemistry Students ?
The Tree of Life: Three Domains
The Six Kingdoms of Life
Six kingdoms
• Archaea
• Bacteria
• Protista
• Fungi
• Plantae
• Animalia
Cellular organization
Unicellular prokaryote
Unicellular prokaryote
Unicellular eukaryote
Uni- or Multicellular eukaryote
Multicellular eukaryote
Multicellular eukaryote
Prokaryote and Eukaryote Cells
Biochemists Grind-Up Cells to get the Molecules of Life
Centrifugation Separates Light from Heavy
The Liver
Cell Fractionation
Fractionation by Density
Molecules sediment
based on mass + friction
(shape)…measured in
Svedbergs (a
multidimensional unit)
OK, Lets Do Some Practice Clicker Questions
1. You have a solution of 4 nM bovine serum albumin. The
concentration of bovine serum albumin is:
A. 4 x 10-6 M. B. 40 x 10-8 M. C. 0.4 x 10-10 M. D. 4 x 10-9 M.
OK, Lets Do Some Practice Clicker Questions
1. You have a solution of 4 nM bovine serum albumin. The
concentration of bovine serum albumin is:
A. 4 x 10-6 M. B. 40 x 10-8 M. C. 0.4 x 10-10 M. D. 4 x 10-9 M.
m = milli
μ = micro
n = nano
p = pico
f = femto
a = atto
10-3
10-6
10-9
10-12
10-15
10-18
Lets Do Some Practice Clicker Questions
2.
A.
C.
E.
Membrane transport proteins are found in:
Cell membrane. B. Endoplasmic reticulum membrane.
Mitochondrial membrane. D. Nuclear Membrane
All of the above.
Lets Do Some Practice Clicker Questions
3. Which pair below is wrong in showing oxidized/reduced forms
of carbon.
A. R-COOH/R-CHO.
B. R-CH2OH/R-CHO
C. R-CHO/R-CH3
D. R-CH=CH2/R-CH2-CH3
E. All are wrong.
Practice Graphing Questions
Here is a pH titration of a purified but unknown organic molecule in
100mL of water. Examine in the titration carefully.
Graphing Practice Question
4. Which titration graph is the most
meaningful?
A. 1.
B. 2.
Graph 1
Graph 2
Same Data in
Excel
pH Titration Unknown Compound
14
12
Clicker Question: What is
going on? (back to
General Chemistry !)
8
pH
The unknown is a:
a. strong base.
b. strong acid.
c. ester.
d. aldehyde.
e. none of these
10
6
Water
Unk Cpd in
Water
4
2
0
0
100
200
300
400
μL 2M KOH
500
600
Clicker Practice Questions
5. Water at Room Temperature is a(n):
A. Acid. B. Organic compound. C. Metal.
D. Solid. E. None of these.
Clicker Practice Questions
6. 5 μmoles/mL is
A. 5 x 10-6 M. B. 5 x 10-3M. C. 5 x 10-9M.
D. 50 nM.
E. none of these.
Clicker Practice Questions
7. Arrange in order of reduced oxidized forms of nitrogen.
A. Amine/Amide/Nitro.
B. Nitro/Amide/Amine.
C. Nitro/Amine/Amide.
D. Amide/Amine/Nitro.
E. Amide/Nitro/Amine.
Clicker Practice Question
Here is the drug morphine. How many Hydrogen atoms does
it have ?
Where Are They
Here is the drug morphine. How many Hydrogen atoms does
it have ?
Where Are They
Here is the drug morphine. How many Hydrogen atoms does
it have ?
A. 7.
B. 19.
C. 14.
D. 18.
E. 17.
Where Are They
1
1
1
2
1
1
1
1 2
3
2
1
1
1
Here is the drug morphine. How many Hydrogen atoms does
it have ?
A. 7.
B. 19.
C. 14.
D. 18.
E. 17.
More Clicker Questions
9. “femto” is between:
A. milli and nano. B. micro and pico.
C. atto and pico. D. micro and macro.
E. sad and happy.
More Clicker Questions
10. A spherical cell, 1 µm in diameter, has eight molecules of the
protein lacR. What is the concentration of the protein in the cell?
More Clicker Questions
10. A spherical cell, 1 µm in diameter, has eight molecules of the
protein lacR. What is the concentration of the protein in the cell?
Solution: Cell volume = 4/3 πr3 = 4/3 π (5 x 10-7m)3 = 5.24 x 10-19m3
= 5.24 x 10-16 L
8 molecules / (6.02 x 1023 molecules/mole) = 1.33 x 10-23 moles
1.33 x 10-23 moles / 5.24 x 10-16 L = 2.5 x 10-8 M or ?
More Clicker Questions
10. A spherical cell, 1 µm in diameter, has eight molecules of the
protein lacR. What is the concentration of the protein in the cell?
What is 2.5 x 10-8 M?
A. 25 nM. B. 0.025 mM. C. 2.5 µM.
Class Periods
Can Start with an iClicker Quiz on the Lecture
and
End of Chapter Problems (EOC problems).
These are Graded Activities that are 1/3rd of your
Grade!
Some classes will have a Case Study.
The Case Studies will NOT be on any Exam, but will have
Graded iClicker Questions exercising what you learned
from Narrated Power Point and Interpreting Biochemical
Data and solving Problems for that Case Study.
STUDY to LEARN before EACH CLASS
1. Mindfully Examine (do not passively
listen) the Narrated PowerPoint. ..
Mindfully test yourself on it to make sure
you know this biochemistry…so you can
use it in Class.
2. Do the practice End of Chapter
Problems…be able to do them with the
book closed….
3. Check out the Case Study for that day on
Blackboard (some will not have a Case
Study, most do).
The Course is in Blackboard
and on the Web
http://www.fiu.edu/~bch3033
The Web version Lacks what Blackboard Has:
1. The Lectures as MP4 files on FIU Media
Site.
2. Grade book…you can keep track of how
you are doing.
3. and more.