Laboratory service : Media & reagents : Consulting
Blood agar plate (BA)
Use : For the isolation, cultivation and detection of hemolytic activity of
streptococci, pneumococci and other particular fastidious microorganisms.
Control organisms :
Streptococcus pneumoniae : Good growth, Alpha - hemolysis
Streptococcus pyogenes : Good growth, Beta-hemolysis
Incubate: aerobic 35° C, 24 hrs.
Shelf life : 4 weeks
Price (Baht) : 12 (return plate) , 15 (disposable plate)
Chocolate agar plate (CA)
Use : For the isolation and cultivation of a variety of fastidious microorganism.
Control organisms :
Hemophilus influenzae and Neisseria gonorrhoeae : Good growth
Incubate : CO2 ,35° C ,24 hrs.
Shelf life : 4 weeks
Price (Baht) : 12 (return plate) , 15 (disposable plate)
MacConkey agar plate (MC)
Use : For the selective isolation, cultivation and differentiation of coliformsand
enteric pathogens based on the ability to ferment lactose. Lactose–fermening
organisms appear as red to pink colonies. Lactose-nonfermenting organisms
appear as colorless or transparent colonies
Control organisms :
Escherichia coli : Pink colonies (lactose fermentation)
Proteus mirabilis : Colorless colonies ,no spreading
Enterococcus sp. : No growth
Incubate : Aerobic, 35° C, 24 hrs.
Shelf life : 4 weeks
Price (Baht) : 12 (return plate) , 15 (disposable plate)
Mueller-Hinton agar plate (MHA)
Use : For antimicrobial susceptibility testing of a variety of
nonfastidious, rapidly-growing microorganisms.
Control organisms :
Escherichia coli ATCC25922 , Staphylococcus aureus ATCC 25923,
and Pseudomonas aeruginosa ATCC 27853 : Growth ,Inhibition Zone
followed Standard interpretative
Incubate : Aerobic 35° C, 24 hrs
Shelf life : 4 weeks
Price (Baht) : 12 (return plate) , 15 (disposable plate)
Salmonella-Shigella agar plate (SS)
Use : For the selective isolation and differentiation of pathogenic enteric bacilli,
especially those belonging to the genus Salmonella. This media is not
recommended for the primary isolation of Shigella species. Lactosefermenting bacteria such as Escherichia coli or Klebsiella pneumoniae appear
as small pink or red colonies. Lactose-nonfermenting bacteria such as
Salmonella species, Proteus species and Shigella species appear as colorless
colonies. Production of H2S by Salmonella species turns the center the colonies
black.
Control organisms :
Salmonella typhi : Colorless colonies, black centers.
colonies
Escherichia coli : Pink
Incubate : Aerobic 35° C, 24 hrs
Shelf life : 4 weeks
Price (Baht) : 12 (return plate) , 15 (disposable plate)
Eosin Methylene Blue Agar (EMB)
Use : For the isolation, cultivation and differentiation of Gramnegative enteric bacteria based on lactose fermentation. Bacteria that
ferment lactose, especially the coliform bacterium Escherichia coli, Appear
as colonies with green metallic sheen or blue–black to brown color.
Bacteria that do not ferment lactose appear as colorless or transparent light
purple colonies
Control organisms :
Escherichia coli : Good growth, green metallic sheen.
Klebsiella pneumoniae : Good growth, purple colonies, no sheen.
Shigella flexneri : Good growth, transparent colonies (lactose negative)
Incubate : Aerobic 35° C, 24 hrs
Shelf life : 4 weeks
Price (Baht) : 12 (return plate) , 15 (disposable plate)
Thiosulfate Citrate Bile Salt Sucrose agar plate (TCBS)
Use : For the selective isolation of Vibrio cholerae and Vibrio parahaemolyticus
from a variety of clinical specimens and in epidemiological investigations.
Control organisms :
Vibrio cholerae : Growth (yellow colonies)
Vibrio parahaemolyticus : Growth (green colonies)
Staphylococcus aureus : No growth
Incubate : Aerobic 35° C, 24 hrs
Shelf life : 4 weeks
Price (Baht) : 15 (return plate) , 18 (disposable plate)
Sabouraud Dextrose Agar plate (SDA)
Use : For the cultivation of pathogenic and nonpathogenic fungi, especially
dermatophytes. The medium may be made more selective for fungi by
the addition of specific antibiotics such as chloramphenicol. For the cultivation
of yeast and filamentous fungi.
Control organisms :
Aspergillus flavus, Candida albicans : Growth
Streptococcus pneumoniae : No growth
Incubate : Aerobic 35° C, 24 hrs
Shelf life : 4 weeks
Price (Baht) : 15 (return plate) , 18 (disposable plate)
Trypticase soy broth for Hemoculture
Use : For the cultivation of a wide variety of fastidious and
nonfastidious microorganisms from blood specimen and in epidemiological
investigations. Also used for rapid estimation of the bacteriological quality of
water.
Control organisms :
Staphylococcus aureus : Good growth
Uninoculated tube : No growth and clear
Incubate : 5% CO2, 35° C , 24 hrs
Shelf life : 8 weeks
Price (Baht) : 10 for 45 ml of TSB (return bottle) , 8 for 27 ml of TSB (return
bottle)
Triple Sugar Iron agar slant (TSI slant)
Use : For the differentiation of members of the Enterobacteriaceae base on
their fermentation of lactose, sucrose and glucose and the production of H2S.
Control organisms :
Escherichia coli : A/A (G)
Shigella flexneri : K/A
Proteus vugaris : K/A, H2S
Pseudomonas aeruginosa : K/N
Incubate : Aerobic 35° C, 24 hrs
Shelf life : 8 weeks
Price (Baht) : 5 (return tube)
Motility Indole Lysine Medium (MIL slant)
Use : For the cultivation and differentiation of members of the
Enterobacteriaceae on the basis of motility, lysine decarboxylase activity, lysine
deaminase activity and indole production.
Control organisms :
Proteus vulgaris : Motile / Indole/ LDC/ LDM ; +/+/-/+
Klebseilla pneumoniae : Motile / Indole/ LDC/ LDM ; -/-/+/Incubate : Aerobic 35° C, 24 hrs
Shelf life : 8 weeks
Price (Baht) : 5 (return tube)
Christensen Urea Agar slant
Use : For the differentiation of a variety of microorganisms, especially
members of the Enterobacteriaceae, aerobic actinomycetes, streptococci and
nonfermenting Gram-negative bacteria, on the basis of urease production.
Control organisms :
Proteus mirabilis : Pink throughout (positive)
Klebsiella pneumoniae : Pink slant (partial positive)
Escherichia coli : Yellow (negative)
Incubate : Aerobic 35° C, 24 hrs
Shelf life : 8 weeks
Price (Baht) : 5 (return tube)
Simmons’ Citrate Agar slant
Use : For the differentiation of Gram-negative bacteria on the basis of citrate
utilization. Bacteria which can utilize citrate as sole carbon source turn medium
blue.
Control organisms :
Klebsiella pneumoniae : Growth and blue color (positive)
Escherichia coli : No growth, remains green (negative)
Incubate : Aerobic 35° C, 24 hrs
Shelf life : 8 weeks
Price (Baht) : 5 (return tube)
Bile Esculin Agar slant
Use : For the isolation and presumptive identification of enterococci (Group D
streptococci).
Control organisms :
Enterococcus spp. : Good growth, black
Alpha-Hemolytic Streptococcus, not group D : No growth, colorless on
media
Incubate : Aerobic 35° C, 24 hrs
Shelf life : 8 weeks
Price (Baht) : 5 (return tube)
6.5% NaCl
Use : For for cultivation and differentiation of bacteria based on their ability
to growth on 6.5% NaCl tolerance.
Control organisms :
Enterococcus faecalis : Yellow (positive)
Group D nonenterococci : Remains orange (negative)
Incubate : Aerobic, 35° C , 24 hrs
Shelf life : 8 weeks
Price (Baht) : 5 (return tube)
Pigment medium
Use : For the cultivation and differentiation of Streptococcus agalactiae (Group
B Streptococcus)
Control organisms :
Streptococcus agalactiae : Orange pigment in stabbing (positive)
Beta-streptococcus not gr. A, B or D : Remains colorless (negative)
Incubate : Aerobic, 35° C , 24 hrs
Shelf life : 8 weeks
Price (Baht) : 5 (return tube)
Ornithine Decarboxylase slant (ODC)
Use : For the cultivation and differentiation of bacteria based on their ability
to decarboxylate the amino acid. Bacteria that decaboxylate ornithine turn the
medium turbid purple.
Control organisms :
Proteus mirabilis : Purple (positive)
Klebsiella pneumoniae : Yellow (negative)
Incubate : Aerobic, 35° C , 24 hrs
Shelf life : 8 weeks
Price (Baht) : 5 (return tube)
Selenite-F Broth (SF)
Use : For the isolation and cultivation of Salmonella species from feces
and other specimens.
Control organisms :
Salmonella spp. : Growth
Escherichai coli : No growth
Incubate : Aerobic, 35° C , 24 hrs
Shelf life : 8 weeks
Price (Baht) : 5 (return tube)
Thioglycollate Medium
Use : For the cultivation of both aerobic and anaerobic organisms in the
performance of sterility test.
Control organisms :
Staphylococcus aureus : aerobic growth
Clostridium spp. : anaerobic growth
Incubate : Aerobic 35° C, 24 hrs
Shelf life : 8 weeks
Price (Baht) : 10 (return tube)
Trypicase Soy Broth (TSB)
Use : For the cultivation of a wide variety of fastidious and nonfastidious
microorganisms from clinical specimen and in epidemiological investigations.
Also used for rapid estimation of the bacteriological quality of water.
Control organisms :
Staphylococcus aureus : Good growth
Uninoculated tube : No growth and clear
Incubate : Aerobic, 35° C , 24 hrs
Shelf life : 8 weeks
Price (Baht) : 5 (return tube)
Phenol Red Glucose (PR Glucose)
Use : For the determination of the ability of a microorganism to ferment
glucose. Fermentation is determined by the production of acid –medium turns
yellow.
Control organisms :
Staphylococcus aureus : Growth (yellow)
Micrococcus spp. : Growth (remain orange)
Incubate : Aerobic, 35° C , 24 hrs
Shelf life : 8 weeks
Price (Baht) : 5 (return tube)
Phenol Red Mannitol (PR Mannitol)
Use : For the determination of the ability of a microorganism to
ferment manitol. Fermentation is determined by the production of acid –
medium turns yellow.
Control organisms :
Staphylococcus aureus : Growth (yellow)
Staphylococcus epidermidis : Growth (remain orange)
Incubate : Aerobic, 35° C , 24 hrs
Shelf life : 8 weeks
Price (Baht) : 5 (return tube)
Phenol Red Sucrose (PR Sucrose)
Use : For the determination of the ability of a microorganism to ferment
sucrose. Fermentation is determined by the production of acid –medium turns
yellow.
Control organisms :
Vibrio cholerae : Growth (yellow)
Vibrio parahaemolyticus : Growth (remain orange)
Incubate : Aerobic, 35° C , 24 hrs
Shelf life : 8 weeks
Price (Baht) : 5 (return tube)
Phenol Red Arabinose (PR Arabinose)
Use : For the determination of the ability of a microorganism to ferment
arabinose. Fermentation is determined by the production of acid –medium
turns yellow.
Control organisms :
Enterococcus faecium : Growth (yellow)
Enterococcus faecalis : Growth (remain orange)
Incubate : Aerobic, 35° C , 24 hrs
Shelf life : 8 weeks
Price (Baht) : 5 (return tube)
Phenol Red Lactose (PR Lactose)
Use : For the determination of the ability of a microorganism to ferment
lactose. Fermentation is determined by the production of acid –medium turns
yellow.
Control organisms :
Incubate : Aerobic, 35° C , 24 hrs
Shelf life : 8 weeks
Price (Baht) : 5 (return tube)
Oxidative Fermentation Glucose (OF Glucose )
Use : For differentiating Gram-negative bacteria based upon determining the
oxidative and fermentative metabolism of glucose. Bacteria that ferment
glucose turn the medium yellow.
Control organisms :
Pseudomonas aeruginosa : Yellow (positive)
Acinetobacter lwoffii : Remains green
Incubate : Aerobic 35° C, 24 hrs
Shelf life : 8 weeks
Price (Baht) : 5 (return tube)
Oxidation Fermentation Maltose (OF Maltose)
Use : For differentiating Gram-negative bacteria based upon determining the
oxidative and fermentative metabolism of maltose. Bacteria that ferment
glucose turn the medium yellow.
Control organisms :
Stenotrophomonas maltophilia : Yellow
Pseudomonas aeruginosa : Remains green
Incubate : Aerobic 35° C, 24 hrs
Shelf life : 8 weeks
Price (Baht) : 5 (return tube)
Lowenstein-Jensen Medium
Use : For the cultivation and differentiation of Mycobacterium species.
M.tuberculosis appears as granular ,rough, dry colonies. M.kansasii appears as
smooth to rough photochromogenic colonies. M.gordonae appears as smooth
yellow- orange colonies. M.avium appears as smooth, colorless colonies.
M.smegmatis appears as wrinkled, creamy white colonies. Also used for the
cultivation and maintenance of Gordona species, Nocardia species,
Rhodococcus species, and Tsukamurella paurometabolum.
Control organisms :
Mycobacterium phlei : Good growth
Uninoculated slant : No growth
Incubate : Aerobic 35° C, 3 days
Shelf life : 4 weeks
Price (Baht) : 10 (return tube)
Stuart Transport medium
Use : For transport of swabs of clinical material containing upper
respiratorytract, enteric pathogens and other fastidious organisms.
Control organisms :
Streptococcus pyogenes : Growth
Uninoculated medium : No growth
Incubate : Aerobic 35° C, 24 hrs
Shelf life : 8 weeks
Price (Baht) : 5 (return tube)
Kovac's reagent
Use : For determination of bacteria that posses the enzyme tryptophanase are
capable of cleaving tryptophan, thereby producing indole, pyruvic acid and
ammonia. Indole can be detected in tryptophan medium by observing
the development of red color after adding a solution containing pdimethylamino-benzaldehyde. (Kovac's reagent)
Control organisms :
Escherichia coli : Red (Indole positive)
Klebsiella sp. : remained yellow (Indole negative)
Shelf life : 1 year
Price (Baht) : 400 per 100 ml
Gram's stain
Use : For differentiating Gram-positive and negative bacteria.
Control organisms :
Escherichia coli : red cell (Gram negative)
Staphylococcus aureus : dark purple cell (Gram positive)
Shelf life : 1 year
Price (Baht) : 400 per set (Crystal violet, Gram's iodine, Decolorizer and
Safranin ; each 100 ml)
Acid fast stain
Use : For the determination of the Mycobacterium, Nocardia, and
Corynebacterium
Control organisms :
Mycobacterium tubercurosis : red cell
Escherichia coli : blue cell
Shelf life : 1 year
Price (Baht) : 500 per set (Carbon fuchsin, 3% Acid alcohol and Methylene
blue ;each 100 ml) Carbon fuchsin only 3 per ml.