Inquiry into Life Twelfth Edition

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Enzymes B11

• Reference: chapter 5 of your text

• Quiz Wed March 31

• Test Thurs April 1 st

• Very short unit, builds on what you already know

• Pay close attention to the study guide you were given (with answer key)

Metabolic Reactions and

Energy Transformations

• Metabolism is the sum of all the chemical reactions that occur in a cell.

A + B C + D

(reactants) (products)

6.2 Metabolic Reactions and

Energy Transformations

• ATP: Energy for Cells

– ATP stands for adenosine triphosphate , the common energy currency for cells.

– ATP is generated from ADP (adenosine diphosphate) + an inorganic phosphate molecule ( P )

The ATP Cycle

Metabolic Reactions and

Energy Transformations

• Structure of ATP

– ATP is a nucleotide that is composed of:

• Adenine (a nitrogen-containing base)

• Ribose (a 5-carbon sugar)

• Three phosphate groups

Metabolic Reactions and

Energy Transformations

• Structure of ATP

– ATP is a “high energy” compound because a phosphate group can easily be removed.

Metabolic Reactions and

Energy Transformations

• Coupled Reactions

– The energy released by an exergonic reaction is used to drive an endergonic reaction.

– Exergonic releases energy, endergonic needs energy “put in”

Coupled Reactions

Metabolic Pathways and

Enzymes

• Metabolic pathways are a series of linked reactions.

– These begin with a specific reactant and produce an end product

Metabolic Pathways and

Enzymes

• Enzymes are usually proteins that function to speed a chemical reaction.

– Enzymes serve as catalysts

A Metabolic Pathway

Metabolic Pathways and

Enzymes

• The Energy of Activation (E a

) is the energy that must be added to cause molecules to react with one another.

Energy of Activation

Metabolic Pathways and

Enzymes

• How Enzymes Function

– Enzyme binds substrate to form a complex

– E + S  ES  E + P

Enzymatic Action

Metabolic Pathways and

Enzymes

• How Enzymes Function

– Enzyme binds substrate to form a complex

– E + S  ES  E + P

– Induced fit model

• Substrate and active site shapes don’t match exactly

• Active site is induced to undergo a slight change in shape to accommodate substrate binding

Induced Fit Model

Metabolic Pathways and

Enzymes

• Factors Affecting Enzymatic Speed

– Substrate Concentration

– Temperature and pH

– Enzyme Activation

– Enzyme Inhibition

– Enzyme Cofactors

Metabolic Pathways and

Enzymes

• Substrate Concentration

• Enzyme activity increases as substrate concentration increases because there are more collisions between substrate and enzyme

• Maximum rate is achieved when all active sites of an enzyme are filled continuously with substrate

Metabolic Pathways and

Enzymes

• Temperature

– Enzyme activity increase as temperature rises

– Higher temperatures cause more effective collisions between enzymes and substrates

– High temperatures may denature an enzyme, inhibiting its ability to bind to substrates

The Effect of Temperature on the Rate of Reaction

Metabolic Pathways and

Enzymes

• pH

• Each enzyme has an optimal pH

• Enzyme structure is pH dependent

• Extremes of pH can denature an enzyme by altering its structure

Effect of pH on the Rate of

Reaction

Metabolic Pathways and

Enzymes

• Enzyme Activation

– Cell regulates metabolism by regulating which enzymes are active

– Genes producing enzymes can be turned on or off to regulate enzyme concentration

– In some cases a signaling molecule is used to activate an enzyme

Metabolic Pathways and

Enzymes

• Enzyme Inhibition

– Occurs when enzyme cannot bind its substrate

– Activity of cell enzymes is regulated by feedback inhibition

– Ex: when product is abundant it binds to the enzyme’s active site and blocks further production

– When product is used up, it is removed from the active site

– In a more complex type of inhibition, product binds to a site other than the active site, which changes the shape of the active site

– Poisons are often enzyme inhibitors

Feedback Inhibition

Metabolic Pathways and

Enzymes

• Enzyme Cofactors

– Molecules which help enzyme function

– Copper and zinc are examples of inorganic cofactors

– Organic non-protein cofactors are called coenzymes

• Vitamins are often components of coenzymes

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