Ribosome Structure
1. Outline the structure of
a ribosome based on the
diagram:
●
●
●
●
A site
Ribosome Structure
1. Outline the structure of
a ribosome based on the
diagram:
● large and small subunits
● A, P, E sites in large
subunit
● mRNA binding site in
small subunit
● composed of protein and
rRNA (ribosomal RNA)
Polyribosomes
2. Define polyribosome
based on the diagrams.
Polyribosomes
2. Define polyribosome
based on the diagrams.
A cluster of ribosomes
translating the same
mRNA transcript.
Peptide Bonds
Ribosomes catalyze peptide bonds between
amino acids.
3. Is this a condensation or hydrolysis
reaction?
Peptide Bonds
Ribosomes catalyze peptide bonds between
amino acids.
3. Is this a condensation or hydrolysis
reaction?
Peptide Bonds
4. Draw the product of peptide bond
formation below.
Peptide Bonds
4. Draw the product of peptide bond
formation below.
Peptide Bonds
5. Which of the following correctly shows a
peptide bond?
Peptide Bonds
5. Which of the following correctly shows a
peptide bond?
tRNA Structure
6. Outline the structure of
a tRNA molecule based on
the diagram:
●
●
●
tRNA Structure
6. Outline the structure of
a tRNA molecule based on
the diagram:
● amino-acid attachment
site (3’ end)
● anticodon (base pairs
with codon)
● clover-leaf, 3 loop
structure (due to Hbonding)
tRNA Activating Enzymes
Amino acids must be bound to the correct tRNA
molecules by activating enzymes.
7. What is the energy source for this process?
tRNA Activating Enzymes
Amino acids must be bound to the correct tRNA
molecules by activating enzymes.
7. What is the energy source for this process? ATP
tRNA Activating Enzymes
8. How is enzyme specificity important in this
process? (Hint: There are 20 different
activating enzymes.)
tRNA Activating Enzymes
8. How is enzyme specificity important in this
process? Each enzyme can bind only 1 type of
tRNA and 1 type of amino acid - this ensures
that the genetic code is followed.