Translation
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In This Lesson: Transcription and Translation (Lesson 2 of 3) Today is Monday, rd November 23 , 2015 Pre-Class: Which DNA base(s) is/are purine(s)? Which is/are pyrimidine(s)? Which is/are neither? Today’s Agenda • • • • Transcription Translation Bingo! The longest protein (and longest “word”) yet known. • Where is this in my book? – P. 190 and following… By the end of this lesson… • You should be able to identify the detailed structures of RNA. • You should be able to describe the process by which DNA is transcribed into mRNA and is then ultimately translated into proteins. The Story • It’s the year 1830. • You live in Bucks County, but own a factory in Lancaster County. The factory is waiting for your instructions before they begin producing anything. • How will you tell your factory to begin without going all the way over there yourself? Think of a way with your partner and be detailed but efficient. The Cell’s Version • • • • • • You: Nucleus Factory: Ribosome Messenger: mRNA Message: Gene Product: Protein The Process of Writing and Delivering the Message: Transcription • The Process of the Factory Making Product: Translation The Big Idea • Today we’re talking about transcription and translation. • Transcription is the process of creating RNA (specifically mRNA). • Translation is using mRNA (and other kinds of RNA) to make proteins. • We’re going to start with transcription. The Big Idea (or Central Dogma) Transcription DNA Translation RNA Protein Trait Replication NOTE: DNA Replication does not have to happen before transcription. Just one more… • Transcription takes place where DNA is, obviously. – In which part of the cell does transcription occur? – The nucleus! Whoo! • Translation takes place in the cytoplasm with the help of a ribosome. The Big Idea (or Central Dogma) Transcription DNA Nucleus Translation RNA Protein Cytoplasm @ Ribosome Trait But first… • Try numbers 1 and 2 on your Transcriptlate Lab. • Find your group’s DNA strand and record it in line 1 on the worksheet. • Then, in line 2, record the complementary strand. – Remember, A to T, T to A, C to G, G to C. About RNA… • RNA is another kind of nucleic acid. – Ribonucleic acid. • It is usually singlestranded, DNA is double-stranded. • Instead of having Deoxyribose, it has Ribose. • Instead of having Thymine, it has Uracil. Mmm, sugar! • Sugar differences: – Deoxyribose is missing an oxygen. http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/P/Pentose.gif Compare and Contrast • Using these pictures and your notes, organize a T-chart comparing DNA and RNA. Here’s what I got… DNA RNA Bases A, C, G, T A, C, G, U Sugar Deoxyribose Ribose Strands Two One Transcription Specifics • Really, you can think of transcription as the nucleus’s way of sending a message to the ribosome to make protein. • It just needs to transcribe the message into ribosome language first. That language is called mRNA. • mRNA stands for “messenger RNA” – makes sense, huh? Transcription • Just like in replication, the DNA is unwound, but this time by RNA Polymerase. • RNA nucleotides begin to be added to a new mRNA strand. – This time, not by DNA Polymerase but……………….. – RNA Polymerase again! (the enzyme that does it all) • Difference: Every time the mRNA molecule would have a T, a U for Uracil is placed instead. – Uracil is also a pyrimidine. Transcription P Deoxyribose ---H--Thymine---H--- P Deoxyribose P P P ---H--Cytosine---H--- Ribose Guanine Guanine Deoxyribose P mRNA DNA exits re-coils nucleus RNA RNAPolymerase Polymerase Strands move breaks makes apart H-bonds mRNA Deoxyribose P P Ribose Thymine Uracil ---H--Adenine---H--- Deoxyribose Deoxyribose Ribose Adenine Adenine Examples • Board examples • Videos – HHMI – Biologix Transcription Steps Know these! 1. Unzip DNA Helix with RNA Polymerase. – [DNA Replication uses DNA Helicase] 2. Create strand of mRNA with RNA Polymerase. – Where you would put a T for Thymine, place a U for Uracil. – [DNA Replication uses DNA Polymerase] Transcriptlate Lab • Now try number 3 on your Transcriptlate Lab. • Remember, use your original strand (printed on your worksheet) to TRANSCRIBE the mRNA and anytime you would put a T, put a U instead. • Note: DO NOT use Strand 2 here. Use Strand 1 to make a complementary Strand 3 (of mRNA), and put it on the lowest line for #3. Sense and Antisense Strands • This is confusing! • The antisense strand is the one being transcribed. – The antisense strand is sometimes called the nonsense strand. • The sense strand is the other one. It looks a lot like the mRNA but has nothing to do with it directly. – Why? ---H--Cytosine---H--- Ribose Deoxyribose Uracil ---H--Adenine---H--- hymine Deoxyribose P uanine P Ribose Guanine P P P ---H--Thymine---H--- Ribose Adenine Deoxyribose Sense Strand denine Antisense Strand Transcription Animation • [Video] Now for Translation • Translation is when the ribosome uses the mRNA strand to make a protein. – The ribosome links together amino acids based on the sequence found in the mRNA. • Quick Reminder: – Protein Monomer: Amino Acid – Protein Polymer: Polypeptide (a string of amino acids) • Quick Summary: – The two halves of the ribosome assemble around mRNA. – The ribosome “reads” the mRNA and calls over the appropriate amino acids. Why do we need translation? • Just as the word “translation” normally means converting between languages, it means almost the same thing for the cell. • It’s sort of like the nucleus speaks in DNA and the rest of the cell speaks in proteins. • RNA and the ribosomes play the role of the translator. Get those Amino Acid tables out! • Every three mRNA bases the ribosome reads is called a codon. – For example: • AUG • UUU • UCG • Each codon corresponds to a particular amino acid. – With a few exceptions. One last thing… • Protein translation always starts with the codon called AUG. To which amino acid does this correspond on your table? – By the way, methionine can also appear in the middle of the amino acid sequence. It just has to be at the front. • Protein translation always stops on one of three codons called “stop codons”: – UAA – UAG – UGA Translation Example • Here’s my mRNA sequence: – CGGAUGCUGAAGUGAGGC • Now here it is with the codons separated: – CGG | AUG | CUG | AAG | UGA | GGC • Now here it is when the amino acids linked together: – Methionine-Leucine-Lysine • Notice how I did not start at the beginning (not a start codon) and stopped at the stop codon. Let’s try it… • Try #4 on your Transcriptlate Lab. • Remember, regardless of how your strand starts, your first amino acid must be methionine. • No matter how your strand ends, stop when you reach a STOP codon. • Write your amino acids above your mRNA strand in Line 3. Let’s try it… • Two Activities: – First is a review – Build a DNA Molecule • http://learn.genetics.utah.edu/content/begin/dna/builddna/ • Do this until you get a message about how fast your rate is. – Second is new information – Transcribe and Translate a Gene • http://learn.genetics.utah.edu/content/begin/dna/transcrib e/ • Do this until you finish it, then put your computer(s) away. Last details on translation… • The amino acids that the cell strings together are originally each attached to a second kind of RNA. – tRNA (for transfer RNA) • tRNA delivers amino acids to the ribosome. – The ribosome adds the amino acids to the growing polypeptide chain (with peptide bonds) and the tRNA leaves. • The ribosome is also made of RNA (and proteins), but a different kind. – rRNA (for ribosomal RNA) • Picture next slide… tRNA and Anticodons • tRNA has a funny shape in most diagrams, but the important thing to know is that at one end is an anticodon. • An anticodon is three tRNA bases that are the opposite of the codon. Translation Steps Know these! 1. Ribosome arrives at mRNA. 2. mRNA is pulled through the ribosome. 3. Start codon (methionine) signals tRNA to start arriving. 4. One tRNA matches up to the codons on mRNA to deposit an amino acid. 5. Another tRNA comes in at the next codon and adds another amino acid to the chain. 6. Process continues until stop codon is reached. At that point the polypeptide is complete. Translation Mechanism MET MET ISO PRO tRNA tRNA Start Codon (Methionine) E Site UP Site A C This process continues until a stop codon is reached, at which point the mRNA strand, tRNA units, and rRNA subunits all are released. A U GA Site U G G Large Ribosomal Subunit (rRNA) A G C A A U G A U A C C C mRNA U G A A U A Small Ribosomal Subunit (rRNA) rRNA (Ribosome) Sites • A Site: – Aminoacyl tRNA site • Where the new amino acid/tRNA complex arrives (except the first one). • P Site: – Peptidyl tRNA site • Where the growing peptide chain is held. • E Site: – Exit site. Side Note: Polyribosomes • Polyribosomes, also known as polysomes or ergosomes, are “structures” made of one mRNA strand and multiple ribosomes. • So in reality, when translation happens, it’s not just one ribosome per mRNA strand. – That would be too slow. • Polyribosomes speed up translation! http://medical-dictionary.thefreedictionary.com/_/viewer.aspx?path=dorland&name=polyribosome.jpg Replication, Transcription, Translation DNA DNA Antisense Strand Sense Strand mRNA Codon tRNA Anticodon Amino Acid TTG AAC AAC UUG Asparagine AGT TCA UCA AGU Serine TCG AGC AGC UCG Serine ACA TGT UGU ACA Cysteine ACC TGG UGG ACC Tryptophan The Big Idea • Don’t forget the big idea: What are Transcription and Translation? – Think of getting a prescription. • Transcription is when the doctor gives you the note and you take it to the pharmacy. – mRNA is made in the nucleus and moves to the ribosome. • Translation is when the pharmacist gives you the medicine. – mRNA is read by the ribosome and proteins are made. And now it’s time for… • Codon Bingo! • Everyone needs a copy of the universal genetic code and a blank bingo card. • Write all twenty amino acids on the card. There are more than twenty spaces so some will have to be written twice (none can be written more than twice). – Don’t include stop. • I’ll give you a DNA, mRNA, or tRNA sequence – you transcribe/translate it to an amino acid. – Be sure you’re correct! Transcription/Translation Key Terms • • • • • • • • • • Codon Anticodon mRNA rRNA tRNA Start codon Stop codons Transcription Translation What else? Closure • Translate the following mRNA codons and submit your results in the turn-in box. – AUG (don’t forget the amino acid name) – CAA – AUC – UGU – CCC – UGA Closure Notes • Where does translation happen? – The cytoplasm • What structure is responsible for performing translation? – The ribosome Closure Protein • The longest protein known to us right now is called Titin in short. • It’s got between 27,000 and 33,000 individual amino acids on it. • Multiply this by three to get a rough estimate of how many mRNA nucleotides are involved. • What does it do? – Helps you contract muscles. • And now…the full chemical name, which…again…was shortened to “Titin.” – Note: Next slide in size 1.9 font. – http://www.sarahmcculloch.com/luminaryuprise/2009/longest-word/ Methionylthreonylthreonylglutaminylarginyltyrosylglutamylserylleucylphenylalanylalanylglutaminylleuc yllysylglutamylarginyllysylglutamylglycylalanylphenylalanylvalylprolylphenylalanylvalylthreonylleucylgl 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hreonylthreonylalanylglutamylthreonylleucylaspartylalanylthreonylarginylarginylvalylaspartylaspartylal anylthreonylvalylalanylisoleucylarginylserylalanylasparaginylisoleucylasparaginylleucylvalylasparagin ylglutamylleucylvalylarginylglycylthreonylglycylleucyltyrosylasparaginylglutaminylasparaginylthreonylp henylalanylglutamylserylmethionylserylglycylleucylvalyltryptophylthreonylserylalanylprolylalanyltitinme thionylglutaminylarginyltyrosylglutamylserylleucylphenylalanylalanylisoleucylcysteinylprolylprolylaspar tylalanylaspartylaspartylaspartylleucylleucylarginylglutaminylisoleucylalanylseryltyrosylglycylarginylgly cyltyrosylthreonyltyrosylleucylleucylserylarginylalanylglycylvalylthreonylglycylalanylglutamylasparagin ylarginylalanylalanylleucylprolylleucylasparaginylhistidylleucylvalylalanyllysylleucyllysylglutamyltyrosyl asparaginylalanylalanylprolylprolylleucylglutaminylglycylphenylalanylglycylisoleucylserylalanylprolylas partylglutaminylvalyllysylalanylalanylisoleucylaspartylalanylglycylalanylalanylglycylalanylisoleucylseryl glycylserylalanylisoleucylvalyllysylisoleucylisoleucylglutamylglutaminylhistidylasparaginylisoleucylglut amylprolylglutamyllysylmethionylleucylalanylalanylleucyllysylvalylphenylalanylvalylglutaminylprolylmet hionyllysylalanylalanylthreonylarginylacetylseryltyrosylserylisoleucylthreonylserylprolylserylglutaminyl phenylalanylvalylphenylalanylleucylserylserylvalyltryptophylalanylaspartylprolylisoleucylglutamylleucyl leucylasparaginylvalylcysteinylthreonylserylserylleucylglycylasparaginylglutaminylphenylalanylglutami nylthreonylglutaminylglutaminylalanylarginylthreonylthreonylglutaminylvalylglutaminylglutaminylpheny lalanylserylglutaminylvalyltryptophyllysylprolylphenylalanylprolylglutaminylserylthreonylvalylarginylphe nylalanylprolylglycylaspartylvalyltyrosyllysylvalyltyrosylarginyltyrosylasparaginylalanylvalylleucylaspar 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ylalanylalanylleucylprolylleucylasparaginylhistidylleucylvalylalanyllysylleucyllysylglutamyltyrosylaspar aginylalanylalanylprolylprolylleucylglutaminylglycylphenylalanylglycylisoleucylserylalanylprolylaspartyl glutaminylvalyllysylalanylalanylisoleucylaspartylalanylglycylalanylalanylglycylalanylisoleucylserylglycy lserylalanylisoleucylvalyllysylisoleucylisoleucylglutamylglutaminylhistidylasparaginylisoleucylglutamyl prolylglutamyllysylmethionylleucylalanylalanylleucyllysylvalylphenylalanylvalylglutaminylprolylmethion yllysylalanylalanylthreonylarginylacetylseryltyrosylserylisoleucylthreonylserylprolylserylglutaminylphen ylalanylvalylphenylalanylleucylserylserylvalyltryptophylalanylaspartylprolylisoleucylglutamylleucylleuc ylasparaginylvalylcysteinylthreonylserylserylleucylglycylasparaginylglutaminylphenylalanylglutaminylt hreonylglutaminylglutaminylalanylarginylthreonylthreonylglutaminylvalylglutaminylglutaminylphenylala 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ltyrosylglutamylserylleucylphenylalanylalanylglutaminylleucyllysylglutamylarginyllysylglutamylglycylala nylphenylalanylvalylprolylphenylalanylvalylthreonylleucylglycylaspartylprolylglycylisoleucylglutamylglu taminylserylleucyllysylisoleucylaspartylthreonylleucylisoleucylglutamylalanylglycylalanylaspartylalanyll eucylglutamylleucylglycylisoleucylprolylphenylalanylserylaspartylprolylleucylalanylaspartylglycylprolylt hreonylisoleucylglutaminylasparaginylalanylthreonylleucylarginylalanylphenylalanylalanylalanylglycylv alylthreonylprolylalanylglutaminylcysteinylphenylalanylglutamylmethionylleucylalanylleucylisoleucylar ginylglutaminyllysylhistidylprolylthreonylisoleucylprolylisoleucylglycylleucylleucylmethionyltyrosylalany lasparaginylleucylvalylphenylalanylasparaginyllysylglycylisoleucylaspartylglutamylphenylalanyltyrosyl alanylglutaminylcysteinylglutamyllysylvalylglycylvalylaspartylserylvalylleucylvalylalanylaspartylvalylpro lylvalylglutaminylglutamylserylalanylprolylphenylalanylarginylglutaminylalanylalanylleucylarginylhistidy lasparaginylvalylalanylprolylisoleucylphenylalanylisoleucylcysteinylprolylprolylaspartylalanylaspartylas partylaspartylleucylleucylarginylglutaminylisoleucylalanylseryltyrosylglycylarginylglycyltyrosylthreonylt yrosylleucylleucylserylarginylalanylglycylvalylthreonylglycylalanylglutamylasparaginylarginylalanylalan ylleucylprolylleucylasparaginylhistidylleucylvalylalanyllysylleucyllysylglutamyltyrosylasparaginylalanyl alanylprolylprolylleucylglutaminylglycylphenylalanylglycylisoleucylserylalanylprolylaspartylglutaminylv alyllysylalanylalanylisoleucylaspartylalanylglycylalanylalanylglycylalanylisoleucylserylglycylserylalanyli soleucylvalyllysylisoleucylisoleucylglutamylglutaminylhistidylasparaginylisoleucylglutamylprolylglutam yllysylmethionylleucylalanylalanylleucyllysylvalylphenylalanylvalylglutaminylprolylmethionyllysylalanyl alanylthreonylarginylacetylseryltyrosylserylis oleucylthreonylserylprolylserylglutaminylphenylalanylvaly lphenylalanylleucylserylserylvalyltryptophylalanylaspartylprolylisoleucylglutamylleucylleucylasparagin ylvalylcysteinylthreonylserylserylleucylglycylasparaginylglutaminylphenylalanylglutaminylthreonylgluta minylglutaminylalanylarginylthreonylthreonylglutaminylvalylglutaminylglutaminylphenylalanylserylgluta minylvalyltryptophyllysylprolylphenylalanylprolylglutaminylserylthreonylvalylarginylphenylalanylprolylgl ycylaspartylvalyltyrosyllysylvalyltyrosylarginyltyrosylasparaginylalanylvalylleucylaspartylprolylleucyliso leucylthreonylalanylleucylleucylglycylthreonylphenylalanylaspartylthreonylarginylasparaginylarginyliso leucylisoleucylglutamylvalylglutamylasparaginylglutaminylglutaminylserylprolylthreonylthreonylalanylg lutamylthreonylleucylaspartylalanylthreonylarginylarginylvalylaspartylaspartylalanylthreonylvalylalanyli soleucylarginylserylalanylasparaginylisoleucylasparaginylleucylvalylasparaginylglutamylleucylvalylarg inylglycylthreonylglycylleucyltyrosylasparaginylglutaminylasparaginylthreonylphenylalanylglutamylser ylmethionylserylglycylleucylvalyltryptophylthreonylserylalanylprolylalanyltitinmethionylglutaminylarginy ltyrosylglutamylserylleucylphenylalanylalanylisoleucylcysteinylprolylprolylaspartylalanylaspartylaspart ylaspartylleucylleucylarginylglutaminylisoleucylalanylseryltyrosylglycylarginylglycyltyrosylthreonyltyros ylleucylleucylserylarginylalanylglycylvalylthreonylglycylalanylglutamylasparaginylarginylalanylalanylle ucylprolylleucylasparaginylhistidylleucylvalylalanyllysylleucyllysylglutamyltyrosylasparaginylalanylalan ylprolylprolylleucylglutaminylglycylphenylalanylglycylisoleucylserylalanylprolylaspartylglutaminylvalylly sylalanylalanylisoleucylaspartylalanylglycylalanylalanylglycylalanylisoleucylserylglycylserylalanylisole ucylvalyllysylisoleucylisoleucylglutamylglutaminylhistidylasparaginylisoleucylglutamylprolylglutamyllys ylmethionylleucylalanylalanylleucyllysylvalylphenylalanylvalylglutaminylprolylmethionyllysylalanylalan ylthreonylarginylacetylseryltyrosylserylisoleucylthreonylserylprolylserylglutaminylphenylalanylvalylphe nylalanylleucylserylserylvalyltryptophylalanylaspartylprolylisoleucylglutamylleucylleucylasparaginylval ylcysteinylthreonylserylserylleucylglycylasparaginylglutaminylphenylalanylglutaminylthreonylglutamin ylglutaminylalanylarginylthreonylthreonylglutaminylvalylglutaminylglutaminylphenylalanylserylglutamin ylvalyltryptophyllysylprolylphenylalanylprolylglutaminylserylthreonylvalylarginylphenylalanylprolylglycy laspartylvalyltyrosyllysylvalyltyrosylarginyltyrosylasparaginylalanylvalylleucylaspartylprolylleucylisoleu cylthreonylalanylleucylleucylglycylthreonylphenylalanylaspartylthreonylarginylasparaginylarginylisoleu cylisoleucylglutamylvalylglutamylasparaginylglutaminylglutaminylserylprolylthreonylthreonylalanylglut 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ylalanylalanylisoleucylaspartylalanylglycylalanylalanylglycylalanylisoleucylserylglycylserylalanylisoleu cylvalyllysylisoleucylisoleucylglutamylglutaminylhistidylasparaginylisoleucylglutamylprolylglutamyllysyl methionylleucylalanylalanylleucyllysylvalylphenylalanylvalylglutaminylprolylmethionyllysylalanylalanylt hreonylarginylacetylseryltyrosylserylisoleucylthreonylserylprolylserylglutaminylphenylalanylvalylpheny lalanylleucylserylserylvalyltryptophylalanylaspartylprolylisoleucylglutamylleucylleucylasparaginylvalylc ysteinylthreonylserylserylleucylglycylasparaginylglutaminylphenylalanylglutaminylthreonylglutaminylgl utaminylalanylarginylthreonylthreonylglutaminylvalylglutaminylglutaminylphenylalanylserylglutaminylv alyltryptophyllysylprolylphenylalanylprolylglutaminylserylthreonylvalylarginylphenylalanylprolylglycylas partylvalyltyrosyllysylvalyltyrosylarginyltyros ylasparaginylalanylvalylleucylaspartylprolylleucylisoleucylt 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rolylleucylasparaginylhistidylleucylvalylalanyllysylleucyllysylglutamyltyrosylasparaginylalanylalanylprol ylprolylleucylglutaminylglycylphenylalanylglycylisoleucylserylalanylprolylaspartylglutaminylvalyllysylal anylalanylisoleucylaspartylalanylglycylalanylalanylglycylalanylisoleucylserylglycylserylalanylisoleucylv alyllysylisoleucylisoleucylglutamylglutaminylhistidylasparaginylisoleucylglutamylprolylglutamyllysylmet hionylleucylalanylalanylleucyllysylvalylphenylalanylvalylglutaminylprolylmethionyllysylalanylalanylthre onylarginylacetylseryltyrosylserylisoleucylthreonylserylprolylserylglutaminylphenylalanylvalylphenylala nylleucylserylserylvalyltryptophylalanylaspartylprolylisoleucylglutamylleucylleucylasparaginylvalylcyst einylthreonylserylserylleucylglycylasparaginylglutaminylphenylalanylglutaminylthreonylglutaminylgluta minylalanylarginylthreonylthreonylglutaminylvalylglutaminylglutaminylphenylalanylserylglutaminylvalyl 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