Option C - Biochemistry Cell Respiration Use the videos to help you answer questions: Cell Respiration Overview: http://www.youtube.com/watch?v=iXmw3fR8fh0&feature=related Songs: http://www.youtube.com/watch?v=jJvAL-iiLnQ&feature=related http://www.youtube.com/watch?v=sCNc0OY__tM Glycolysis http://www.youtube.com/watch?v=ub1zTkZL5sE&feature=related http://www.youtube.com/watch?v=x-stLxqPt6E Link Reaction http://www.youtube.com/watch?v=-LO9pP3dF3Q KREBS CYCLE http://www.youtube.com/watch?v=3wCGkmfEV9I&feature=related http://www.youtube.com/watch?v=aCypoN3X7KQ ATP Synthesis http://www.youtube.com/watch?v=KXsxJNXaT7w&feature=related http://www.youtube.com/watch?v=1engJR_XWVU&feature=related http://www.youtube.com/watch?v=QHmdJtiaNYg&feature=related http://www.youtube.com/watch?v=Idy2XAlZIVA&feature=related http://www.youtube.com/watch?v=StXlo1W3Gvg&feature=related 1. Draw and label a mitochondrion (use the diagram as a guide) 2. List the four stages of Cellular respiration: i. ii. iii. iv. 1 3. State where glycolysis occurs. 4. In the table, indicate the four stages of glycolysis. Stages of Glycolysis Outline each stage of Glycolysis Two phosphate groups are added to 1 glucose molecule to create 1 hexose biphosphate molecule. The phosphate groups are provided by 2 ATP molecules. The hexose biphosphate molecule is broken into 2 molecules of triose phosphate. Two atoms of hydrogen are removed from each triose phosphate molecule and added to 2 molecules of NAD+. Pyruvate is formed by transferring 2 phosphate groups from each triosephosphate molecule to 4 molecules of ADP. 5. Put all these pieces together to show the reactions and products of glycolysis. 6. Complete the table to show the number of each product of glycolysis Product of Glycolysis Number of molecules produced Pyruvate ATP NADH H+ 2 7. Fill in the blanks: hint OIL RIG oxidation is the ………..……………….. of electrons from an element reduction is the ………………………… of electrons oxidation frequently involves gaining ………………….. or losing …………………… reduction frequently involves losing ………………………. or gaining ……………………. 8. Put all these pieces together to show the reactions and products of glycolysis. 9. Quiz: True or False i. Glycolysis occurs in the mitochondrion ii. Glycolysis produces 1 pyruvate molecule. iii. Most of the energy that was stored in the glucose molecule is still present in the 2 pyruvate molecules. iv. When oxygen is present, pyruvate is transported to the mitochondrial matrix v. vi. vii. viii. The link reaction forms a link between glycolysis and the Krebs cycle. The link reaction involves oxidative decarboxylation of pyruvate The link reaction involves the reduction of NADH to NAD+ The link reaction produces acetyl coenzyme A (acetyl CoA) and CO2. 10. Put all these pieces together to show the citric acid cycle. 3 11. Complete the table to show the number of each product of glycolysis Product of Glycolysis Number of molecules produced CO2 ATP NADH + H+ FADH2 12. Quiz: True or False i. ii. iii. iv. v. vi. The Krebs cycle is a series of reactions that occurs in a mitochondrion. The Krebs cycle begins with the entry of a 4-carbon molecule. The first 6-carbon product in the Krebs cycle is citrate. CO2 is removed in 1 of the reactions of the Krebs cycle ATP is produced directly in 2 of the reactions (substrate-level phosphorylation) For each glucose molecule (at the start of glycolysis) there will be 2 turns of the Krebs cycle. 13. Fill in the blanks The last step of aerobic respiration is called ………………………………………….. In this step electrons and hydrogens from ……………………….. and …………………………. are passed along an ……………………………………………chain from one carrier molecule to another. The electron carriers are strategically located in the ……………………………………. membrane of the …………………………….. They are mainly proteins that shift between ……………… and ………… states as they accept and donate electrons. The final electron carrier is ………………………….. which bonds with H+ to form water. As the electrons are passed down the electron transport chain, ……………………….. are pumped across the membrane into the ……………………………… space. This creates a ……………………………., which is a store of potential energy, and in turn powers the synthesis of ATP. The inner membrane of a mitochondrion is impermeable to protons but they flow back into the matrix through ATP …………………………………. molecules that are embedded in the membrane. As the ions flow down the gradient, …………………………… is released and used to phosphorylate ADP. When ATP synthesis is coupled to a concentration gradient of H+ it is called…………………….. 4 14. Put all these pieces together to show the electron transport chain. 15. Complete the table to show the functions of the mitochondrion’s structures. Mitochondrion Function . Matrix Inter- membrane space Cristae 5 16. Complete the cellular respiration crossword 6