Genetics QUIZ GENETIC CORN LAB Carolina Biological 1: Pollination Carolina Biological 1: Pollination FILE Carolina Biological: 2 Harvesting Carolina Biological: 2 Harvesting FILE Carolina Biologica: 3 Preparing the seeds Carolina Biologica: 3 Preparing the seeds - FILE Where do seeds come from? Tassel (flower with pollen) Silk (flower with eggs) 1. Describe the corn plants – at least three facts. 2. Ask at least one question. 3. Write two characteristics – one inherited, one that is not. 4. Two traits? Phenotypes? 6.Letters? 5. Which phenotypes are recessive and which – dominant? Explain. 7. COUNT each phenotype. Ratio. 8. Compare to Mendel’s predictions. Trait 1: Trait 2: Phenoty Phenot Phenoty Phenoty pe 1: ype 2: pe 1: pe 2: Num ber of plant s: Rati o: Chlorophyll A Chlorophyll-binding protein Dwarf No GA effect Gibberrelinic acid (GA) Normal GA effect LAB: Answer questions 1-8, including your counts. 1. Describe the corn plants – at least three facts. 2. Ask at least one question. 3. Write two characteristics – one inherited, one that is not. 4. Two traits? Phenotypes? 6.Letters? 5. Which phenotypes are recessive and which – dominant? Explain. 7. COUNT each phenotype. Ratio. 8. Compare to Mendel’s predictions. Journal: Two Tall Green corn plants were crossed, giving rise to the seeds we had planted. 1. Looking at the seedlings, what must be the genotypes of the parents? (Choose the letters) 2. What would be the expected phenotypic ratio in the children? Explain. G g G GG Gg g Gg gg Green: White ¾:¼ T t T TT Tt t Tt tt Tall: Dwarf ¾:¼ G - Green g - White T – Tall, t – Dwarf For each trait, the phenotypic ratio is ¾:¼ Is this what your counts show? NOTES Notes: Mendel’s Law of Independent Assortment Looking at two traits at a time “Allele pairs separate independently of each other during gamete formation by meiosis.” When does this law work? Only when the genes are situated on different chromosomes (not linked) Assort Independently: A and C; B and C Do NOT assort Independently: A and B - they are on the same chromosome. This will affect the ratios between the phenotypes in F2 generation… Are the two genes, for color and for height sitting on the same chromosome? If they are NOT, and they assort independently, then we can allow ourselves to: A. List ALL the possible gamete combinations in the punnet sq. B. Multiply individual probabilities. GgTt x GgTt G - Green g - White Possible phenotype T – Tall, Combinations: t – Dwarf Green, Tall Green, Dwarf White, Tall White, Dwarf If the two traits are inherited independently, what are their expected ratios? A. PUNNET SQUARE G = Green, g = White plant For Corn…. T = Tall, t = Dwarf GgTt x GgTt All the possible gametes: GT Gt gT gt GT Step 2: Placing gametes in Gt the punnet square gT gt What is the expected phenotypic outcome? GT YySs x YySs GT GGTT Gt GGTt gT GgTT gt GgTt Gt GGTs GGtt GgTt Ggtt gT GgTT gt GgTt GgTt ggTT ggTt Ggtt ggTt ggtt Expected phenotypic outcome: Green Tall 9/16 : Green Dwarf White Tall 3/16 : 3/16 : White Dwarf 1/16 Now, compare these predictions to your actual counts! B. MULTIPLYING PROBABILITIES G g G GG Gg g Gg gg G - Green g - White T – Tall, t – Dwarf Green: White ¾:¼ For each trait, the T t phenotypic ratio is ¾ : T TT Tt ¼ t Tt tt Is this what your Tall: Dwarf counts show? ¾:¼ Are the probabilities of the combined phenotypes, the products of the individual chances? Green, Tall Green, Dwarf White, Tall White, Dwarf ¾ x ¾ = 9/16 ¾ x ¼ = 3/16 ¼ x ¾ = 3/16 ¼ x ¼ = 1/16 Is this what we got in our counts? 9 3 3 1 Expected phenotypic outcome: Green Tall 9/16 : Green Dwarf White Tall 3/16 : 3/16 : White Dwarf 1/16 Now, compare these predictions to your actual counts! 1. Write the possible gametes in the following parent: AaBbCc (notice: one allele per gamete for each genes: ABC, Abc, …) 2. Repeat your answer considering the position of the genes on the chromosomes: Assort Independently: A and C; B and C Do NOT assort Independently: A and B - they are on the same chromosome. This will affect the ratios between the phenotypes in F2 generation… Assignment: Answer ALL question in the packet up to 15. For extra 40 points – continue to question 19! (For number 12 – draw the chart, calculate the ratios, by turning the largest count into 9, and dividing the rest by the same factor). ACTUAL COUNTS? Draw the following chart for the F2 generation, count the plants and fill in the numbers you got: Green Tall Green Dwarf White Tall White Dwarf Draw the following chart for the F2 generation, count the plants and fill in the numbers you got (2 classes): Green Green White White Tall Dwarf Tall Dwarf 82 27 30 4 / 9.11= / 9.11 = / 9.11 = / 9.11 = 9 Complete Lab – Part TWO Part THREE, looking at the REAL gene mapping on the corn chromosomes, can be done at home, for extra credit!