Monkemeier
Honors Biology
January 5, 2015 – January 9, 2015
Microscope Skills / Lab Unit
Name _______________________ Date __________________
Microscope Skills / Microscope Lab Study Guide for Quiz
Quiz Dates:
Periods 2 and 4 – Friday, January 9, 2015
Periods 5 and 7 – Thursday, January 8, 2015
1. Identify and state the functions of the parts of the microscope highlighted within your Microscope
Lab.
2. Define and/or explain the following terms as they relate to a compound light microscope: Parfocal,
Magnification, Resolution, Depth of Field, Refraction, Field of View, Scanning Lens, Low Power
Objective, High Power Objective, Oil Immersion Lens.
3. Explain and/or demonstrate the proper handling methods: carrying, table placement, cleaning, and
putting away.
4. Explain the correct steps to create a wet mount slide.
5. Explain the correct steps to stain a slide.
6. Explain the correct steps to locate and focus an image starting with scanning lens through oil
immersion lens.
7. Explain the how the orientation of the specimen differs from the orientation of the image when
viewed under the microscope
8. Calculate the total magnification of the microscope. (see Mathematical Models below)
9. Explain what happens to the: field of view, magnification, resolution, brightness when switching
from lower magnification to higher magnification.
10. Explain how to determine different depths when using a microscope. Which depth will come into
focus first under scanning or low power? How will you determine which part of the image is closest
to the coverslip? Is closest to the slide?
11. Measure the field of view in mm under low power and convert to microns. See Below
12. Given the field of view under low power, calculate the field of view in microns under high power. See
Below
13. Explain why indicating the magnification on the drawing as well as maintaining the amount of field
of view that is occupied by image, are both important and required when sketching images of
specimens viewed under microscope.
14. Determine the approximate size of a cell, cell part, or image under the microscope when
information relating to the magnification and field of view are provided.
15. Explain why the coarse adjustment is never used under high power magnification.
16. Explain why the image viewed may be “lost” when moving from low magnification to high
magnification and provide the steps for correcting this problem.
17. Explain the relationship between the following terms: (THINK DEEPLY)
a.
b.
c.
d.
Refraction and the diaphragm or light
condenser
Resolution and magnification
Parfocal and coarse adjustment
Parfocal and fine adjustment
e.
f.
g.
Parfocal and high power magnification
Refraction and Oil Immersion lens
Field of view and diaphragm or light
condenser
18. MATHEMATICAL MODELS:
a.
b.
c.
Total Magnification = (Ocular Lens Magnification) x (Objective Lens Magnification)
1 millimeter = 1000 microns
High Power Field Size = lower power magnification
X
Low Power Field Size in Microns
High Power Magnification