Page # 129-130 131-132 133-134 135-136 137-138 139-140 141-144 145-146 147-150 151-152 153-154 155-156 157-158 159-168 169-172 173-178 179-180 Unit 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 Description Transcription & Translation Review Notes: Translation Notes: Mutations DNA to Disease Activity Lab: Sickle Cell Anemia Notes: Prokaryotic Genetics & Gene Regulation Operon Study Guide Notes: Eukaryotic Gene Regulation Modeling Regulatory Switches of the PITX1 gene Notes: Eukaryotic Cell Differentiation Thalidomide Article Notes: Virology Restriction Enzyme Activity Notes: Biochemistry Transformation Lab Restriction Enzyme Analysis Lab Microarray and PCR virtual Labs Virtual Labs – Link on my website For both labs answer the following questions: Describe the purpose & applications of the technique. Summarize the procedure for how the test works For Microarray ONLY: How can you tell if a gene is turned “on” or “off”? What What’s cells? specifically is being measured by the microarray? the difference between cancerous and healthy For PCR ONLY: of: primers, (Thermus aquaticus) Taq polymerase, & nucleotides. Why do you perform a negative control? Role From: http://learn.genetics.utah.edu/content/labs/