The Theory of Evolution A theory… Explains the current observations and predicts new observations. Present day organisms are similar, but not identical, to fossil organisms. •Explain this observation •What predictions can we make? •We should see observable differences and similarities between fossils and living things. •Others? A Theory… What types of evidence would help us confirm these predictions? •Fossils •Embryology •DNA sequence •Similar Body Structures Variation & Adaptation OBJECTIVES: • Define species • Distinguish between variation and adaptation • Understand the concept of a niche • Explain how fossils are used to study ancient life forms What is a species? A species is a group of organisms that can mate and produce fertile offspring. Recall: Species • Organisms that can mate and produce fertile offspring. • Variation can exist within a species: – Polymorphism- 2 or more distinct forms. Ex; peppered moth; males and females. – Geographic variation – Unique local environments favor particular traits. – Individual variation Variations can be dramatic (fur color of foxes) or subtle (different blood types of penguins) Most variations are genetically determined and therefore inheritable. These inherited variations are the result of two random processes: 1) Mutation – a change in genetic information 2) Recombination – shuffling the genes or alleles Genetic Determination of Variations • Mutations: Changes in sequence of DNA bases. May occur during – replication – meiosis Sickle Cell Anemia Mutation in the gene that codes for hemoglobin. Beneficial in some environments because being heterozygous for sickle cell = immune to malaria Most variations, however, result not from mutations but instead from the RECOMBINATION of existing alleles. Alleles can be recombined by three different mechanisms. 1) Independent assortment of chromosomes 2) Crossing-over during meiosis 3) Combination of genes from egg & sperm ADAPTATIONS Variations create a population with diverse traits. These traits ultimately determine whether an individual will survive in an environment. An adaptation is an inherited trait that increases a population’s chances of survival and/or reproduction in a particular environment. Adapatations are traits that help organisms survive in their niche. Therefore all members of the population possess the trait (adaptation). All giant anteaters have the same noticeable adaptations. 1) long snout for prodding ant hills 2) long tail for balance 3) sharp claws for tearing through (Myrmecophaga tridacttyla) termite mounds AND 4) long sticky tongue for capturing insects Types of Adaptations Anything that helps an organism • Hide from/defend against predators • Attract a mate/reproduce (sexual selection) • Catch food Broadly speaking, a niche is a habitat and the role a population plays in that habitat. A niche includes where organisms live, what and how they eat, how they raise their offspring, and what their predators are. When niches overlap, competition ultimately results. Lions & hyenas Summary of Variation & Adaptation : 1) Most variation in a population results from mutation and recombination of existing alleles. 2) Some variations are adaptations that increase a population’s chances for survival and reproduction. 3) Each species in an environment has its own niche. Niches are created and destroyed by environmental changes. Darwin and His Theory OBJECTIVES: • Explain the theories that led to the theory of evolution. • Understand the importance of Darwin’s Voyage on the HMS Beagle. • Analyze Darwin’s theory of evolution by natural selection • Distinguish between gradualism and punctuated equilibrium Beginning in the late 1700’s paleontologists were beginning to discover fossils of animals that no longer existed. Charles Bonnet 1769, Swiss naturalist Observed that fossils do not resemble modern organisms. Theorized periodic catastrophes: affect the entire planet killing all life, which began anew after each catastrophe. First to use the term “evolution” to mean change over time. Jean-Baptiste Lamarck Lamarck believed that fossils of extinct animals were the ancestors of animals living today. 1. Organisms constantly strive to improve themselves. 2. The most-used body structures develop, unused structures waste away. “Use and Disuse” 3. Once a structure was modified by use or disuse, modification is inherited by offspring. This third principle is called the “inheritance of acquired characteristics (Lamark stated that a giraffe’s neck gradually became longer due to stretching it during its lifetime. Their longer necks could then be passed on to their offspring.) Lamark’s hypothesis about the inheritance of acquired characteristics was later disproved by German biologist August Weismann Through experiments with mice, Weismann concluded that changes in an individual during its lifetime DO NOT usually affect its reproductive cells or its offspring. Darwin’s Story On January 10th, 1831, Darwin just 22 years old, set sail around the world on the H.M.S. Beagle as .its naturalist. The voyage lasted longer than he expected. He returned to England five years later! . The Voyage of the Beagle (1831-1836) The Beagle’s mission was to undertake a survey voyage to South America in order to produce more accurate maps. One of the locations they visited that influenced Darwin the most was the Galapagos Islands. “They are situated under the equator, and between five and six hundred miles westward of the coast of South America.” Aboard the “H.M.S. Beagle”, Darwin spent five weeks exploring the Galapagos Islands Each of the islands, although having similar climates and environments and being only about 50 miles apart, has its own fauna and flora - similar to but distinct from those of the neighboring islands. This suggested to Darwin that the similar species might have developed from a common ancestor rather than each having been created separately. Influences on Darwin The Earth is very old. Thomas Malthus “Human populations are growing so fast, that the supply of resources cannot keep up with demand. This results in war, starvation and disease.” Darwin realized that this principle applied to all species, not just humans. Thomas Malthus (Population Biologist) All organisms compete for resources. “In October, 1838, I happened to read Malthus for amusement. Being well prepared to appreciate the struggle for existence, which everywhere goes on, from long continued observation of the habits of animals and plants, it at once struck me that under these circumstances favorable variations would tend to be preserved and unfavorable ones to be destroyed. The result of this would be the formation of a new species.” Artificial Selection Evolution can be seen experimentally through artificial selection. Artificial Selection is an experiment in Quantitative Trait Loci A single trait affected by multiple genes and measured on a continuous scale. Ex: size, feather color, weight Over generations of artificial selection, gene pool is altered. Several genes are affected. Corn has been selected to produce larger and larger ears over the past 200 years. All dogs breeds were created from wolves by humans over a 10,000 year period. Darwin’s Theory of “Natural Selection” 1. There is variation within populations. Some variations are favorable. 2. More offspring are born than can possibly survive due to limited resource. 3. Individuals that survive and reproduce are those with favorable traits. 1858, Darwin received a manuscript from Alfred Russel Wallace entitled “On the Tendency of Varieties to Depart Indefinitely from the Original Type.” Wallace had independently reached the same conclusion that natural selection had played a major role in the origin of new species. Dismayed, Darwin offered to withdraw his own manuscript, but a joint paper by the two men was read before the Linnaean Society of London on July 1, 1858. Wallace had also gone on an observational expedition to an area of the world filled with isolated islands. On November 24, 1859, Darwin’s book, “On the Origin of Species ” is published. Chapter 16 Population Genetics • • • • • OBJECTIVES: Relate the study of genetics to that of population genetics and discuss factors that can affect gene-pool equilibrium Explain the Hardy-Weinberg model Discuss evolution through natural selection Explain genetic drift and contrast its effects on large and small populations. Discuss the role of quantitative traits in microevolution. Population Genetics Recall: variation among individuals allows populations to adapt to new environmental conditions or to be selectively bred for desirable traits. 2 Types of Evolution • Microevolution: change within a species. Occurs over dozens or hundreds of generations* • Macroevolution: Much longer time period. Results in a new species A More Precise Definition Microevolution is a change in the genetic composition of populations. Studied by population geneticists. Gene pool All alleles in a population of organisms. Allele frequency Percentage of a particular allele in one population. Ex: In a population of pea plants that are all homozygous for purple flowers, allele freq. for purple flowers is 100% A change in an allele frequency is an indication of evolutionary change. Allele Frequencies within Beetle Population Polymorphic Populations • Have 2 or more alleles for a particular trait. • Ex: humans are polymorphic for blood type. • Ex: apple trees are polymorphic for fruit color. Hidden Genetic Variations • Mutation in non-coding regions of DNA • Silent mutations code for the same amino acid • Unseen polymorphisms The Hardy-Weinberg Model An idealized mathematical model of gene pools. •Mathematician Godfrey H. Hardy •Physicist Wilhelm Weinberg Hardy-Weinberg Equilibrium Allele and genotype frequency will stay constant in the absence of disturbing influences. p2 + 2pq + q2 = 1 • Good news for Darwin! (Assumed blending inheritance.) Hardy-Weinberg Model Makes some assumptions about the population. No “disturbing influences.” • random mating • no mutation (the alleles don't change) • no migration or emigration • infinitely large population size • no selective pressure for or against any traits. Hardy-Weinberg Equilibrium Predictions of the model 1. Predicts allelic and genotypic frequencies 2. Genetic variation remains in the population (unless selective pressures) Genetic equilibrium: a constant state of allele frequency The following conditions must be met in order that genetic equilibrium not be disrupted. 1) No natural selection 2) Random mating 3) No migration 4) No mutation 5) Large population size Note: does not occur in nature. So, why use Hardy-Weinberg model? Quick Quiz 1. Would a change in allele frequencies be more likely to produce microevolution or macroevolution? 2. What is the difference between gene pools and allele frequencies? 3. Why does the concept of gene pools apply to populations but not to species? A Normal Distribution results from Stabilizing Selection: Natural selection that favors average individuals in a population. Disruptive Selection: Natural selection that favors either extreme trait. Disruptive Selection in Snails Limpets with light-colored shells blend in with light rocks and sand. Dark shells blend in with dark rocks. Limpets with medium-colored shells are easily seen on both rocks and eaten by birds. Disruptive Selection in Spiders • When spiders are small, they are not as easily seen by predators. • When spiders are large, they are often too big to be eaten. • Spiders in the middle are the most vulnerable to predators. Other Factors Affecting Gene Pools • Gene Flow: Migration to a new population, organism may bring new alleles with it. • Mutation: If beneficial, will be favored by natural selection and gradually increase in frequency • Genetic Drift: Spontaneous changes in allele frequencies. Small populations only. Other Factors Affecting Gene Pools • Inbreeding: Gradual increase of homozygotes. Ex: California Condor • Population bottleneck: Population size reduced for a few generations. Inbreeding results. Ex: Buffalo in the 1800’s. • Inbreeding increases frequency of harmful recessive alleles. Leads to Inbreeding Depression: reduced fertility and survival. Did you know… The average human is estimated to have 7 alleles that would be lethal if they were homozygous? In inbred populations, inheriting 2 of these alleles is more likely. (BSCS Biology: A Molecular Approach) Population Genetics CONCEPT REVIEW: • Evolution results from a disruption in genetic equilibrium. • The normal distribution of variations in a population can be changed by natural selection, gene flow, mutations, and genetic drift Chapter 17 The Origin of Life Objectives: • Describe the origin of the universe and probable conditions on early Earth • Evaluate hypotheses about the origin of life and identify the probable characteristics of early life-forms • Distinguish between chemical and biological evolution • Describe the fossil record for prokaryotes and eukaryotes. The Big Bang The Expanding Universe • Edwin Hubble, 1920. The Hubble Telescope was named for him. • Wavelengths of light can be measured, spread out as objects move farther away • The rate of expansion is known, used to calculate the time when universe was tiny. The Big Bang • 15 million years ago • Universe was condensed into a tiny “singularity” • An infinitely hot, dense mass. • When it exploded, The Big Bang, hurled energy and mass into space. • What was there before the Big Bang? Early Earth • 4.6 Billion years ago • Meteorites and the oldest rocks from the Moon confirm this History of the Earth Era Million Years Ago First evidence of Cenozoic 7-5 65 Human-like apes Primates Mesozoic 140 220 235 Flowering Plants Mammals Dinosaurs Paleozoic 300 360 400 430 520 Reptiles Amphibians Land Animals Land Plants Vertebrates Precambrian 2100 2500 3500 Eukaryotes Free O2 in atmosphere by prok. Prokaryotes The Early Atmosphere • Gasses from volcanoes: N2, CO2, H2O, H2, CO, probably ammonia (NH3) and methane (CH4) • No O2 • No ozone layer – intense radiation, extreme temperature changes. The First Living Things • Anaerobic Organisms • 1 billion years later, some photosynthetic organisms began releasing free oxygen. How did those living things come to be? 3 possible explanations: 1. Life originated on some other planet, then traveled to Earth through space. 2. Life originated by unknown means on Earth 3. Life evolved from nonliving substances through interaction with their environment. 2 of these cannot be tested, only one can be stated as a hypothesis. Which one? Chemical Evolution Life evolved from nonliving substances • Small, inorganic molecules were heated via cosmic radiation, volcanoes, radioactivity and lightning. • Gasses in the atmosphere react, forming organic compounds • Compounds accumulate in oceans, forming a hot soup • Life evolved by chemical reactions and transformations in the organic soup Chemical Evolution The oceans became “soup” of organic compounds The Heterotroph Hypothesis The first living things were probably heterotrophs that fed on organic compounds in the ocean. With no competition, autotrophs would not have an advantage over heterotrophs The Heterotroph Hypothesis (or Oparin-Haldane hypothesis) 3 Requirements 1. There had to be a supply of organic molecules, produced by nonbiological processes. 2. Some processes had to assemble those small molecules into polymers such as nucleic acids and proteins. 3. Other processes had to organize the polymers into a system that could replicate itself Evidence for the Heterotroph Hypothesis Stanley Miller’s experiment in 1950. Early Earth conditions were simulated in an airtight apparatus. • Water vapor • Lightning • CH4, NH3, H2O, H2 After circulating for a week, new compounds were found in the water, including some amino acids. Other Sources of Organic Molecules • Meteorites from space – amino acids have been discovered • Volcanic vents – release gases at high temperatures Remember 1st requirement: There had to be a supply of organic molecules, produced by nonbiological processes. The rest of the hypothesis: #2. Some processes had to assemble those small molecules into polymers such as nucleic acids and proteins. Clay – repeating crystalline structure that could attract then connect monomers #3. Other processes had to organize the polymers into a system that could replicate itself RNA – Can form spontaneously. Can reproduce itself. Probably came before DNA Biological Evolution • When did chemical evolution become biological evolution? • When organic molecules became living things • Self reproduction, mutation that can be inherited, and natural selection • Cells? Today all living things are made of cells. • It is unknown when/how cell membranes developed. • The first living things may have had membranes, or not. They may have been DNA, RNA, proteins…who knows? Prokaryotic Fossils 3.5 Billion years old. Single-celled prokaryotes. Suggest life was already diverse and thriving. Probably methanogens Fossils of Eukaryotes • 2.1 Billion years old • Lynn Margulis of UMass, Amherst developed the endosymbiont hypothesis: Chloroplasts and mitochondria were once free-living prokaryotes. Photosynthesis and respiration of the small cells have benefited the host cells. • Mitochondria probably evolved from aerobic, heterotrophic purple bacteria. • Plastids probably evolved from autotrophic cyanobacteria. Endosymbiont Hypothesis The evidence: Both have their own DNA and ribosomes, which are similar to other bacteria. Also both have a double membrane; their outer membranes may have evolved from vacuoles when host cells took them in. Quick Quiz 1. Why is it believed that methanogens might have been the first organisms? 2. How might mitochondria and plastids have originated? 3. What evidence supports the idea that mitochondria and plastids originated from free-living prokaryotes? Chapter 18 Diversity and Variation Outcomes • Explain homology and give examples of homologous structure • Describe how the general characteristics of the 5 kingdoms differ. The 5 (or 6) Kingdoms • • • • • • Archaebacteria Eubacteria Protista Fungi Plantae Animalia Bacteria/monera Bacteria • Prokaryotes • First organisms to evolve Protista • • • • • Earliest eukaryotes. Usually single celled. No organ systems Nucleus developed Mitochondria, flagellates, and plastids became incorporated. • Ex: amoeba, paramecium, algae Fungi • • • • Usually multicellular (except yeast) Eukaryotic Heterotrophs Evolved from fungus-like protists (slime molds) • Ex: mushroom, mold, yeast Plantae • Multicellular, with complex body systems (roots, stem, leaves) • Autotrophs • Eukaryotes • Evolved from photosynthetic bacteria • Ex: Flowering plants, Conifer, Mosses, Ferns Animalia • • • • Multicellular with complex systems Heterotrophic Eukaryotic Ex: Fish, Amphibian, Reptile, Bird, Mammal There are still lobe-finned fish today called mudskippers. 34 species have been identified. Unlike those we evolved from, most of today’s species have only 2 appendages (front lobe-fins) Fish to Amphibians Reptiles to Birds Evolution of Mammals Eventually, some mammals returned to the water. • Today’s whales had an ancestor similar to a wolf. Chapter 19 Changes in Species Outcomes: • Cite evidence from fossils, ecology, and homologies that support the theory of evolution • Discuss the genetic and molecular evidence for evolution • Discuss isolation mechanisms that can cause speciation • Describe the patterns in evolution such as punctuated equilibrium 1) When this fish dies, its body sinks to the bottom where it is gradually covered by mud (A). 2) . . . . Its body starts to decay (B), which is caused by bacteria metabolizing the organic molecules of the fish. If the decay goes on long enough there will be nothing left of the fish to be fossilized. This is one reason why fossils are rare; they decay before being fossilized. However, there are locations on the bottom of the ocean with few bacteria. If a fish lands on such a spot, and is quickly covered by mud (C), it may not decay very much. In the mud, the fish body is turned into a fossil by the process of mineral replacement. Water slowly carries away the organic fish molecules and replaces them with minerals (rock) until only mineral remains. . Like a book with pages of stone. Since the animals and plants are buried in sediments at the same time that the sedimentary rock is forming, the fossils in the rock layers must be the same age as the rock layers. By using radioactive dating techniques, scientists can determine the age of volcanic rock layers found between the sedimentary layers and thus the age of the fossils too. Soft-Tissue Fossils Ice in the Arctic has preserved some fossils for 1,000s of years. In 1999 a wooly mammoth was discovered intact. Can it be revived by crossing with an elephant? Different #s of chromosomes (58 and 56), but 95% similar DNA. Scientists have found 250,000 species of extinct orgnisms Estimate that only 1 in 10,000 have been found. Evolution of the Horse In these pictures, there appears to be a straight line progression from the first horse ancetor to the modern horse species. Such a progression implies an evolutionary goal, since there is a trend toward larger body size and fewer toes. Evolution of the Horse However, evolution rarely follows a straight line to a goal. Remember, There are no goals in evolution ! Evidence for Evolution: Body Structures 1) homologous 2) vestigial 3) analogous Homologous Structures: Traits that are similar in different species because they share a common ancestor. Note how the bones have adapted to different niches This is evidence of a common ancestor. Vestigal Structures: No longer used. The Human “Tailbone” This is evidence that humans evolved from an ancestor that had a tail. Vestigal Organ: human appendix appendix A whale has a pelvic bone too, and tiny leg bones. Analogous Structures: structures that are similar in function but are not inherited from a common ancestor. NOTE: Analogous structures indicate that organisms are not related. Embryology is also used as Evidence of Evolution: Similar development of the embryo is evidence of a common ancestor All three embryos have “gill pouches” in the folds of the neck. All three have tails. As these embryos develop, note how the last two continue to look similar. This indicates that they are more closely related to each other than they are to the first embryo The last two are both mammals! This suggests that if traced back far enough all three have a common ancestor because all three have similar looking embryos. This is also evidence of evolution. Perhaps the clearest biochemical evidence of the common origin of living things is the genetic code. The same nitrogen bases of adenine, thymine, guanine, and cytosine exist in every form of life. In addition, the genetic code itself – the codons for the amino acids – is almost universal. The genetic code is the same in every known organism. Every organism uses the same base codes for amino acids Degree of Relatedness Can be determined by • • • • Amino acid sequence Homologous proteins Nucleotide sequence Homologous genes. More Genetic Evidence: Pseudogenes Gene duplication: produces multiple copies of DNA sequences. Pseudogenes: gene copies that don’t function, so aren’t subject to natural selection. Mutations occur unchecked. According to natural selection, these noncoding sections should accumulate mutations faster than functional genes – and they do! Amino Acid Sequence • Can be used to determine relatedness How fast do evolutionary changes take place? Based upon Darwin’s theory it has long been believed that evolutionary changes were slow and gradual: Gradualism. 1972: scientists Stephen Jay Gould and Niles Eldridge advanced a different explanation about the rate of evolution called Punctuated Equilibrium •Punctuated Equilibrium: populations remain genetically stable for long periods of time, interrupted by brief periods of rapid change. •sudden changes in the environment •increased mutation rate. stasis Speciation is the evolution of one or more species from a single common ancestor species. Patterns of Evolution How do species remain separate? (1) Potential mates do not meet. Grizzly and Polar bears. (2) Potential mates meet but do not breed. Nocturnal and diurnal birds. Leopard frog populations that breed during different months. (3) Potential mates meet and breed, but do not produce viable offspring. Divergent Evolution: Occurs when isolated populations of a species evolve independently. Grizzly Bear Polar Bear Divergent evolution is responsible for polar bears. A northern population of grizzly bears became isolated from others of the species and adapted to the Arctic regions. Coevolution: Interactions with other organisms effect evolution. Coevolution is responsible for mimicry one of the most fascinating topics in biological evolution. Coevolution The pronuba moth and the yucca flower Depend on one another for reproduction. Coevolution The Orchid Fly Coevolution: Cactus and Galapagos Tortoise Saddleback shell Adaptive Radiation: Many diversely related species from one common ancestor Polyploidy in Plants If plants inherit an extra chromosome from parents, they are said to be polyploidy. Often, these plants can only mate with other polyploids, or use asexual reproduction. Convergent Evolution: Unrelated species display similar features. No common ancestor. How does this happen? Convergent Evolution Similar niches usually contain similar evolutionary pressures (selective pressures). If ancient niches were similar to modern niches then organisms today could resemble organisms now long extinct. Similar niches found on different continents can produce organisms that are fairly similar. Modern dolphins and prehistoric ichthyeosaurs (marine reptiles) look very similar due to the types of niche they inhabited. Note how similar niches created long necks in both sauropods and giraffes. . . . Quick Quiz 1. What are isolating mechanisms, how do they operate? 2. What is polyploidy? What is its connection to speciation? 3. Explain the statement: “Populations evolve, not individuals within a population.” Origin of Species Concept Review: • New species can develop when populations become separated and isolated. • Similar traits can develop in unrelated species occupying comparable niches. • Interactions with other organisms affect evolution. • Many diverse species can evolve from one ancestral species. Chapter 20 Human Evolution Outcomes: • Describe how modern humans differ from other primates • Evaluate the techniques used to study evolutionary relationships in humans • Compare early hominids with Homo erectus and Homo sapiens • Give reasons for the difference in the gene pools of modern human populations. What are Primates? • • • • Opposable Thumbs Fingers and toes have nails, not claws Flexible shoulder and hip joints Binocular, 3-D vision for accurate depth perception • Color vision Humans vs. Other Primates • Bipedal: Hands are free • Have a hippocampus: brain region for memory and learning. Absent in most primates (not chimps and gorillas) • More fine motor control in hands • Language, well developed vocal chords Molecular Similarities Human vs. Chimpanzee Protein Number of amino acids Amino-acid difference Hemoglobin 579 1 Myoglobin 153 1 Cytochrome C 104 0 Serum Albumin 580 7 Molecular Similarities Among Primates Species compared Difference in DNA sequence (%) Chimpanzee vs. 0.7 Bonobo Human vs. 1.6 Chimpanzee Human vs. 2.3 Gorilla Gorilla vs. 2.3 Chimpanzee Estimated time since divergence 3 million years 7 million years 10 million years 10 million years Early Hominids • Lived in Africa • Genera in Hominid family: Homo and Australopithecus (larger teeth, smaller brains). The Hominids Hominids – The Human-like Primates Comparing Skeletons Skeletal fossils – clues to how organism moves, eats, behaves. Footprint fossils – How organism moved, how heavy it was. Who was Lucy? Comparing Skeletons Skeletal fossils – clues to how organism moves, eats, behaves. Footprint fossils – How organism moved, how heavy it was. Who was Lucy? Australopithecus afarensis found in Ethiopia, 1974 The First Humans Hominids