H&S Reference
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Summary of the Assessment / materials used
precipitation of asphaltenes from heavy crude and the preparation of
metal adducts
precipitation of asphaltenes from heavy crude and the preparation of
metal adducts
reaction of raney nickel with hydrogen peroxide
use of liquid nitrogen to powder fruits and vegetables
use of 0.1M sodium azide stock solution to remove antibodies from
nitrocellulose membranes
preparation of a diet containing 0.005% (or similar concentration) of
Perfluorodecanoic acid (PFDA) and administration to rats
using liquid nitrogen for demonstrations
sol-gel preparation
preparation of Mn exchanged kaolinite, laponite and montmorillonitebased catalysts
synthesis and preparation of alumina and zinc-oxide by co-precipitation
from their mixed nitrate solutions
preparation of manganese-cobalt spinel
preparation of Nickel on charcoal powder
distillation of N,N,N',N'-Tetramethylhexane-1,6,diamine (TMHDA)
analysis of nickel from potassium hydroxide using a solvent extraction
method
long term testing of fuel cell electrodes
addition of potassium carbonate to koh and water to determine
concentrations in koh being used in a fuel cell half cell before and after
testing conditions
to electroplate silver onto a carbon surface using a silver rod and silver
nitrate in water
making of calibration standards to amnalyse the amount of carbonate
dissolved in the koh
acid base titrations to determine ion exchange capacity of different
materials
preparation of tween-80-expanded and brij-series-expanded molybdenum
trioxide
preparation of tween-80-expanded and brij-series-expanded vanadium
oxide
preparation of tween-series-expanded molybdenum trioxide
phenethylthiourea preparation for lc/ms analysis
preparation of au/c/alkaline electrodes for use with alkaline membrane
preparation of tween-series-expanded vanadium oxide
staining agarose gels by gelred nucleic acid gel stain
drying bacterial samples for sem analysis
preparation of tween-60 expanded vanadium oxide
to electroplate silver onto a carbon surface using a silver rod and silver
nitrate in water
analysis of ti-doped apatite materials for their ability to decompose
acetaldehyde in dark, natural and uv-lit conditions.
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infection of tetrahymena pyriformis with mycobacterium bovis
preparation of pt/c/alkaline electrodes for use with solvay membrane
addition of potassium carbonate to koh and water to determine
concentrations in koh being used in a fuel cell half cell before and after
testing conditions
acid base titrations to determine ion exchange capacity of different
materials
cadmium chloride to dose live mammalian cells
grafting of kaolinite clay
influence of lead concentration on iron uptake by caco-2 cells
preparation of magnetic cellulose sponges containing charcoal
adsorbents
fatty acid analysis by gc. transesterification of oil using sodium methylate
and mtbe.
determination of total carbohydrate by colorimetry, using 5% phenol and
h2so4
tert-butyl methyl ether (mtbe) for tranesterification of lipids, fatty acid
analysis by gc.
analysis of arsenic using leucocrystal violet uv/vis assay
analysis of arsenic using rhodamine-b uv/vis assay
analysis of arsenic using azure b uv/vis assay
reaction of n-acetyl-s-(2,3,5-trio-benzoyl-d-ribofuranosyl)-l-cysteine,
workup, tlc and 1h nmr.
risk assessment of a replication-competent reovirus (reolysin tm) vector
for an in-vivo study.
making rossman's fluid and subsequent use to fix liver tissue.
risk assessment of a replication-competent reovirus (reolysin tm) vector
for an in-vivo study.
to produce an etfe-alkaline anion exchange membrane (etfe-aaem) by
converting etfe-g-(poly)vbc to etfe-g-(poly)vinylbenzyltrimethylammonium
chloride
solgel synthesis of hydroxyapatite systems
synthesis of silver doped hydroxyapatite
the precipitation of lanthanum compounds
heating of a polymer sheet in koh and water to see any changes
digestions 1) wet ashing (kjedahl tubes, waterbath and squat beakers) 2)
dry ashing (muffle furnace)
synthesis of proton conducting pval/h3po2/h2o electrolyte membranes
barium chloride in detection of sulphate (4500-so4 e. turbidimetric
method. 1998.
to produce an etfe-alkaline anion exchange membrane (etfe-aaem) by
converting etfe-g-(poly)vbc to etfe-g-(poly)vinylbenzyltrimethylammonium
chloride
long term testing of fuel cell electrodes
2-bromoethanol in ethanol
analysis of nickel from potassium hydroxide using a solvent extraction
method
preparation of polyurethane foam containing charcoal and iron filings
sorption of phenanthrene by charcoal
grafting of vinylbenzene chloride (vbc) onto electron beam activated etfe
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film
simulated gastrointestinal digestion of soy and fish proteins by pepsin and
pancreatine and determination of the angiotensin converting enzyme
(ace) activity of the derived peptides
synthesis of surfactant expanded mn02
synthesis of surfactant expanded vo2
synthesis of vo2 nanotubes
preparation of phosphoranilidates from alcohols
preparation of phosphoranilidates from alcohols
arsenic by molybdenum blue method for uv/vis detection
astm method d2972-03: standard test method for arsenic in water by
uv/vis
mycobacterium tuberculosis (28, 28a, 29ax02)
ethidium bromide solution used for staining nucleic acids (23ax02)
chloroform used in genomic dna extraction (23ax02)
tween 80, used in culture media (23ax02)
working in hplc (dan lab) and probably in 29ay01
nitric acid for use in digestions/sample dilutions/cleaning
analysis of boron using azomethine uv/vis assay -12az03
digestion of samples containing trace elements 28az03
staining of lipids in cultured cells with oil red o
culture of adherent cell lines (e.g. cos-7, at3-1), including use of liquid
nitrogen and virkon
cell culture of 3t3-l1 fibroblast cell line and differentiation into adipocytes
preparation of a nafion mea using pt and pt/ru electrodes
membrane immobilisation of glucose oxidase, membrane casting,
voltammetry
synthesis of poly (ethylene glycol) dimethacrylate
analysis of boron using azomethine uv/vis assay
use of nitric acid in digestion
digestion of supplements in squat beakers
use of ionic liquids for separations and analysis
making up small molecule standards for accurate mass lc-ms
operation and maintenance of ilab 650
automated analysis of creatinine, triglycerides, glucose (oxidase) and
cholesterol using instrumentation laboratory (il) kits, calibrators and quality
control (qc)
preparation of blood samples taken for f2 isoprostane analysis
measurement of ldl and hdl subclasses using iodixanol density gradient
ultracentrifugation
automated analysis of glucose, triacylglyerol and total cholesterol using
randox/ alfa wasserman test kits, calibrators and quality controls.
automated analysis of apolipoproteins, ldl cholesterol and hdl cholesterol
using randox/alfa wasserman test kits, calibrators and quality controls.
use of dry ice (frozen co2) as cooling aid transportation
isolation of human plasma and serum by low speed ultracentrifugation
making up standards for gc and gc-ms
long term testing of fuel cell electrodes
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sodium thioglycollate (incorporated into growth media)
stimulation of gonadotroph cells with [des-gly10, d-ala6]-gnrh ethylamide
herbs extraction (volatile oil and fixed oil) using different solvents
pot trial using soils contaminated with heavy metals, including aresenic,
lead, cadmium, zinc and copper.
synthesis of pigments
low temperature synthesis of apatites, coprecipitation of solutions with
refluxing
inhibition of transcription using actinomycin d
barium chloride and barium sulfate
using resazurin to monitor the redox potential in anaerobic media
formation of aluminium keggin ion and subsequent insertion into layered
clays to form pillared species
methanol-sunflower oil interaction with koh catalyst
drying solvent by refluxing over calcium hydride
synthesis of hydroxyapatite
synthesis of hydroxyapatite 2
chn analysis
making up matrices for maldi-mass spec
preparation and use of carrez a (zinc sulfate heptahydrate) and carrez b
(potassium hexacyanoferrate(ii) trihydrate) reagents, which are used for
protein precipitation and clarification of samples destined for analysis by
hplc or lc-ms(-ms).
hand gun residue testing
synthesis of mno2 nanotubes (permanganate route)
synthesis of g-mno2 nanotubes (permanganate route)
synthesis of mno2 nanotubes
synthesis of moo3 nanotubes
use of chn gases in 25az03
the preparation and testing of pt/c/alkaline electrodes
preparation of bromo resorcarene
making up standard solutions for calibration and specification checking of
mass spectrometers (new user)
antibiotic selection of transfected cells
cleaning and reinstallation of lct mass spec sample cone. 12ay03 and
13ay03
solid phase extraction and hplc analysis of drugs/pesticides
icp-ms analysis of lead and cadmium (nhs - 36ay04)
heat killing of m. tuberculosis/m. bovis for removal from the cl3 laboratory
preparation of diets containing 2500ppm (or similar concentration) of
clofibric acid and administration to rats
preparation of diet containing 1000ppm (or similar concentration) of
nicotinic acid (niacin) and administration to rats
growth and maintemnance of acanthamoeba castellanii
synthesis of calix[4] pyrrole derivative
making up standard solutions for calibration and specification checking of
mass spectrometers
dismantling, cleaning and reassembly of lct mass spectrometer source
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immunisation of mice with neisseria meningitidis
acid and base baths to clean glassware and utensils
formalin disinfection of small samples of mycobacterium
tuberculosis/bovis/bcg pateur
continuous and batch cultures of mycobacterium tuberculosis/bovis/bcg
pateur
cress pytotoxicity trial for diesel contaminated soil
pot trial using contaminated soil from a site contaminated with metals
handling and mixing of contaminated soils
growth of campylobacter jejuni in minimal media with iron sources
growth of campylobacter jejuni in minimal media with carbon sources
staphylococcus aureus (growth and manipulation in the laboratory
listeria monocytogenes (growth and manipulation in the laboratory)
vibrio parahaemolyticus (growth and manipulation in the laboratory
salmonella sp. (growth and manipulation in the laboratory)
enterobacter sakazakii (growth and manipulation in the laboratory)
use of thimerosal (also called thiomersal) as an antibacterial agent
viscosity experiments using acid tar and charcoal
soxhlet extraction: extracting pyrene and phenanthrene from charcoal background compounds
growth of shaken liquid cultures of mycobacteria
preparing gc-ms standards: pahs
preparing gc-ms standards: diesel
isolating polycyclic aramatic hydrocarbon (pah) degrading bacteria from
innoculated charcoal
adding polycyclic aromatic hydrocarbons (pahs) and minerals to charcoal
and innoculating it with pah degrading bacteria
Spraying soil / charcoal isolation plates with PAHs dissolved in ethyl ether
Establishing a large community of diesel (hydrocarbon) degrading
bacteria in charcoal
Forensic Practical "Bloodstains on the ground"
Forensic Chemistry Practical "A Pool of Evidence"
Forensic Chemistry Practical "White Powders"
Preparation of Delipidated Foetal Bovine Serum
Solutions to be reacted with Cu2+
Measurement of hydrogen peroxide using the o-dianisidine colorimetric
method
General risk assessment for the use of atomic absorption spectroscopy
Preparation of diets containing ciprofibrate
3-morpholinosydnonimine in a disc diffusion assay against Campylobacter
jejuni
Cadmium chloride in a disc diffusion assay against Campylobacter jejuni
Chitinase assay of bacteria using 1% chitin TSA plates
Manipulaion of Plesiomonas shigelloides
Measurement of the rate of entry of metal ions into the skin
Analysis of diesel emmitted particles in water as a suspension
Treatment of cells in culture with rapamycin
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Minimum inhibitory concentrations using uric acid with Campylobacter
jejuni and disc diffusion assays with uric acid and C. jejuni
Rapid screening method for the quantition of bacterial cell lipids from
whole cells
Entrapment of sodium tetraphenylborate in PVC membrane
DRAQ5 used for the cromatin staining of cells cultured on a coverslip or a
suspension
Trichloroethyl chloroformate as a hydroxyl protecting group
para-toluene sulphonic acid (hydrate)
Molecular Seive (dust)
Oxalyl chloride for carboxylic acid chloride generation
Hydroxylated and/or methoxylated cinnamic acids for use in synthesis
4-dimethylaminopyridine
Desiccation of tissue sections
6-phosphogluconate
clofibric acid
milk practical
acetamide
oxidation damage to proteins
insect cell tissue culture
autoradiography
Legionella pneumophilia
RNAprotect
cell cytotoxicity assays
nitric oxide measurement
carbohydrate assay
electrochemical synthesis of quantum dots
anti-Campylobacter assay
enzymatic assay
glycerol assay
isolated tissue work using organ baths
effects of R- & S- enantioners on rats
coating the quartz crystal
oxidative stress assay for Campylobacter
analysis of selenium in serum
spray disinfectant for cleaning safety cabinets
preparation of microarray slides
interactions of polyaromatic hydrocarbons
cell staining for oxidative bursts
polycyclic aromatic carbon interactions
extraction of primate nucleic acids
degradation ofo-nitrophenol by fungi
calibration of MALDI-TOF
molecularly imprinted polymers
3-methyl-2-benzothiazolinone hydrazone
clavulanic acid assay
spiking of sand systems
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quinone derivitisation
affinity electrophoresis
COX-2 inhibitors
decolourising culture supernatant
amino/organic acids for Streptomyces
administration of BHT to rats
TLC
HPLC standards
Barry degradation
simple phenolic & polyphenolic compounds
formaldehyde assay
quality assessment standards
measurement of superoxide production
piperazine diacrylamide for 2D PAGE
chromogenic substrates for sulfatase
benzyl mercaptan
nitrosylating agent
methylation
tert-butyl ether
immunogen
receptor binding
extraction of RNA from shellfish samples
glucose assay kit
pyrrolidinedithiocarbamate
PGE2 assay
western blotting
Proteinase K for DNA extraction
incubation of cells with cadmium chloride
hydrogen peroxide determination using QCM
detection of organophosphates with the QCM
section staining for copper with Timm's stain
Greiss assay for nitrate/nitrite determination
culture media additives
detection of hydrogen peroxide
electropolymerised polymer membrane
pyrithione-mediated Zn uptake
LDL-cholesterol receptor assay
phosphorus pentachloride
enzymatic conversion using the QCM
optimisation of membrane composition
catabolism of metalloferrocyanides
identification of aldehydes
phosphorylation
culture media additives
phenol reactions using the QCM
reverse connection polymerase chain reaction
coating internal surfaces of bioreactor
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intracellular Caz+ measurement
addition to soil bacterial cultures
n-acetylcysteine/cinnamon reaction
Carnoy's fluid - fixative
radical generator
anthocyanins & anthocyanidins
purification of myoglobin
Sirius red staining for collagen
Acanthamoeba, Myco bovis
cleaning the quartz crystal
derivitisation of adenosine
effect of cadmium on degradation of PAHs
biological buffer
iron chelating
determination of cholesterol & creatinine
polymer films to modify surface of quartz crystal
inhibition of free radical reactions
iron binding capacity assay kit
spin coating the quartz crystal
1-octanesulphonic acid
extraction & analysis of ascorbic acid
culture media antibiotic
tissue culture additives
antimicrobial activity assessment
casting polydimethylsiloxane films
thioctic acid & pyridoxine
manganese chloride
monomeric phenolic compounds
ammonium bicarbonate
adenosine release studies
biodegredation of benzo[a]pyrene
isocitrate liase assay
vitamin E analysis
solubilisation buffer for protein extraction
transfection of plasmid DNA into cells
a-glycerophosphate dehydrogenase assay
K+ & Cl- channels in rat cells
measurement of lipid hydroperoxides
penicillamine
antioxidant assay
calixarenes & silica to modify quartz crystal
preparation of solution
HPLC standards
cleaning the quartz crystal
immobilisation of RNA/DNA probe
culture media additives
measurement of total antioxidant activity
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effects of second messengers on intestinal nutrient uptake
staining cells
studying anti-tumour activity using cell lines
neurochemical analysis of neuronal function
testosterone hydroxylase assay
extraction of phenanthrene from chicken manure
dilution of phenanthrene
neurochemical analysis of glial cell function
inhibition of protease activity
chemicals used in 33AY04
production of indophenol dye
carcinogens used in 33AY04
harmful/irritant chemicals used in 33AY04
decontamination of soil containing BCG
mutagenesis assay
removal of zinc from bovine serum
extraction of anthocyanins from fruit
iron transport assay
polyacrylamide gel electrophoresis
RNA isolation
thymidine uptake by mammalian cells
cell culture
cell metabolism studies
isolated tissue work using organ baths
anaesthesia
tissue culture additives
aluminium, cobalt,, chromium, copper, gold, iron, lead, manganese,
magnesium, selenium & zinc standard solutions
nickel standard solution
mercury standard solution
cadmium standard solution
arsenic standard solution
Use of bovine serum & horse blood
Protein purification, immunoprecipitation
Iodination of proteins
Electrophoresis, radioimmunoassay
Selective media
Antioxidant assessment
mRNA isolation
AJ buffer
Gas chromatography
Assay for renin inhibitors
Human tumour biopsies
Culture media additives
Effect of chemicals
CHAPS-detergent for protein cells
RNA extraction from mycrobacterium tuberculousis need to be ribolysed
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gel retardation with in-gel DNA foutprinting
extraction of nuclear extraces
cytotoxicity studies with NHEK cells
cleaning of glass sample by sonication in methanol
use in tissue culture assay
use of glycerol for storage of bacterial cultures
use of diethylene glycol as a carbon source for bacteria in mineral water
dimethylsulphoxide (DMSO)
chloroform, hexane, boroutrifluoride/methanol
use of isophthalic acid as a carbon source for the growth of
microorganisms
terephthalic acid
biodegranation of cnude oil
substractive hybridisation
polymerase chain reaction
glutamine transaminase k assay
DNA extraction from agarose/bacterium using KITS
carboxylation of sceleroglucan using sodium periodate and sodium
chlorite and acetic acid
use of 3 amino 1,2,3 triazole in culture media
investigation into the use of pahs as a sole carbon source for
mycobacteria
paraformaldehyde
use of soybean oil in microbiological media
l-chloro-2,4 dinitrobenzene use to detect fusion proteins
nelson assay for soluble sugars
stimulation of cells with staphylococcus enterotoxin from staphylococcus
aureus
rhodanile blue cell culture staining
anti fude
digestion of liunocellulosic material
removal biofilm from mineral water bottles using 1% sodium
pyrophosphate
mutagenic treatment of steptomycetes with ethyl methane sulphoate
use of sodium azide
preparation of serum containing medium (keratinocyte medium) for
human skin cell culture
category 2 viruses
poly-l-lycsine use as fixative for bacteria/cells/tissue prior to staining
use of aprotinin as protease inhibitar
application of nitrapynin (2-chloro-6(trichloromethyl) pynidine) to soil
samples
mistogen induced cell proliferation assay
diatomaceous earth
proteases-brotelain, elastase, endoproteinases glu-c, papain, lchymotrypsin
use of cl-nerf for ph determination in tissue
sonication of mycobacterium bovis bcg using sound chamber
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vancomycin-hcl
tunicamycin
penicillin-G potassium
cinnamic aldehyde
bacitracin
use of virkon as a virucidal disinfectant
preparation of 6% L-naphthol in ethanol to assay diacetyl in yoghurt
n-decyl aldehyde used as substrate
digestion of soil and plant material
use of brays nitrate-nitrite powder in most probable number assay for
nitrogen cycle microorganisms
cellulose type 20
reduction of carboxyls on xanthan using 9-barabicyllo
ammonium metavanadate
antimony, potassium tartrate
treatment of bacteria cells
methyl viologen
reduction of carboxylin acid in xantan gum using barane-tetrahydrofuran
tetrahydrofuran
use of Rnase-away
making up vitamin supplements for gnotobiotic rats
breeding experimentation with rats
human decidnal tissue
superoxide dismutase
titration procedure for catalase assay
trace methal additive for pining water medium
polyvinylpolypyrrolidone-cell culture
production and extraction of patulin
nystatin
handling hazard group 2 micro-organisms
opening of freeze-dried ampoules-containg micro-organisms
modification of sulphydryl group using iodoacetic acid
nitrilotriacetic acid
polysaccharide extracellular
production of Ag/Agci flectrodes by electrolysis
determination of pyruvyl
boiling of boric acid solution
DNA-purification resiri
isolation of human plasma lipoproteins by preparative ultracentifugation
chemical mutagenasis of saccharopolyspora erythraea using methanesulphonic acid ethyl ester
ciprofloxacin
norfloxacin
deacetylation of xanthan and related polymers
fixation of phanti tissue
phasphorus elimation using sodium metabisuphite
listenia monocytogenes
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uranyl acetate
titration for chloride using potassium thiolynate
treatment of listeria monotycogenes with phospodiesterase l type lV
extraction and methylation of bacterial fatty acids
nickel sulphate
wease assay
for producing storage stock solutions of xgal (carbohydrate analogue)
n-octane
cyanida detoxification by fungi
rozoshere remediation of toxins in soil
weighing powdered media
purigication of immunoglobulin from tissue culture supernatant
trolox
modificiation of lipid composision in bacterial cell using myristic acid
iodization of plastic bamphes to reduce microbial cononization of inetrior
surfaces
acetyl determination
used as ph indicator of totrated solution
use of sodium borohydride as a meducing agent in small scale
handling citrinin as a chromatolographic or extraxtion standard
tris (hydroxymethyl)
magnesium oxide
used as standart for calibrate the hygrography
tergitol
measurement of heart rate and blood pressure using mercury
sphygmomanometers
sulphuric acid used to titrate extract solutions for ammonium
determination in soil
silver sulphate-urease assay
adenosine-antyfoamaemulsion, edta acit ATP content in the soil
the effect of pancreatin, trypsin, pronase on the M.I.C of nisin against
listeria monocytogenes
the effect of chymotripsin on the n.i.c of nisin against listeria
monocytogenes
galvinoxyl
minocycline
tetracycline
removed of layer protein using lithium chloride and effect on M.I.C of nisin
ageinst l.monocytogenes
pyruvate determination
hygromycin
RNAzol-b
used as TCL stain
preparation of samples for transmission electron microscopy
patulin
propidium ioade staining of cells
effects of compounds on the replication of cells
assay to detect phagocytosis/cytotoxicity
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determination of non-specific esterase acting in cells
tissue culture and enumeration of cells
formaldehyde solution
DNA extraction from bacterial cells
ammonium iron citrate
using potassium cyanide to inhibit bacteria
effect of potassium metabizulphite on zygosaccharomyces bailli
determination of copper concentration in water samples with sodium
diethyldithiocarbamate
klorextra
negative staining with 1% tungstophosphoric acid
rhodamine 123
mixed lymychocyte culture
electropolishing of coppre electrodes
dapi-cell staining
cyanoditoly tetrazolium chloride or ctc
iso-nicotinic acid hydrazide
4-chloro-napthol-colour substrate for horse darish peroxidase
sbs 218 practical microbial physiology,ecology
dichloran-glycerol agar base containing botran 2,6-cichloro-4-nitro-aniline
extraction of 5-layer proteins with 5m guanidinium hydrochloride
estimation of mic of p.pancimobilis with h2o2 using a ctalase inhibitor 3amino-1,2,4-triazole
extraction of bacteria pigment using acetone
superoxide dismutase
catalase
mycophenolic acid
amonopterin
the use of fatty acid and hydrocarbons as carbon sources for
thraustochytrids
transformation of yeast using lithium acetate and single-standed carrier
DNA, by heat shock
melachite green solution for spore stain
growth of influenza virus in emryonated chicken
rose gittlieb orocess for fat determination in milk
handling potassium cyanide
handling methyl cellosolve
scintillation fluid (optiplasa safe)
nucleon chicago solubilizes (NCS)-tissue solubilizer
determination of heme by fluorescence techniques
fluorescein diacetate-for fluorescent microscope
use of anisaldehyde as visualisasing agent for TLC plates
nisin elisa-the use 3-3-5-5- tetramethylbenzidine di HCL
INT
anisole-side chain deprotection,peptide synthesis
use of hydroethidine for flow cytometric analysis of reactive oxygen
metabolites in mononnuclear cells
346
345
344
344
343
342
339
338
337
336
335
334
332
331
328
328
327
326
325
321
320
320
319
318
317
316
315
314
311
309
308
307
306
304
303
300
299
295
294
293
292
291
290
289
288
286
methyl cellulose for cryopreservation of serum free cell cultures
dichloroacetic acid-supplememntation of cell culture media
supplementation of cell culture media
use of 2,7-dichlorofluoressin diacetate for flow citometric analysis of
reactive oxygen matabolites in mononuclear cells
l-malic acid
substrate for elisa
drug sensitisation of cell line;to induce the growth of tk-cells
cell mutation,skaning nuclei, cell cycli analysis using the falscan
dimethyl sulphoxide used to protect cells when frozen down
use of erythromycin
polyethylene glycol
tetraethyl silicate
to eamine effects on penicillin production
electrical detection of listeria
use of API 20E bacterial identification kits
API reagent
preparation of Ig G fraetion from sheep serum
final year food microbiology practical
2nd year food microbiology practicals
arginine-glucose medium
processing biological materials for electron microscope
industrial microbiology
tetrazolium salt
cobalt chloride
ammonium nitrate
3.5 di-nihosalycilic acid-use in glucose assay
practical physiology and microbiology
practical bacteriology
experiments using endotoxin
horseradish peroxidase
small scale production of printed circuit boards
acetaldehyde
diphenylamine-DNA assay
DNA dipstick test
handling concanavalin-a, con a, or cananalia ensiformis lectins
buthanol,pyridine, water solvent for paper chromatography
quantitative detetrmination of uronic acids 4-hydroxybiphenol
sodium aurothiomalate
5-bromo-4-chloro-3-indolyl beta-d-galactopyranoside
8-hydroxyquinoline
assaying hydrogen peroxide concentrationusing titanium sulphate
assaying superoxides in oil using nitrotetrazolium blue
use of sodium borohydride
use of n-n-dicyclohexylcarbodi-imide as inhibitor of ATPase activity
trypan blue solution
respiration of transformed roots
285
284
282
281
280
274
269
268
267
266
265
264
263
262
261
258
257
256
255
254
253
252
251
250
249
247
246
245
244
243
242
241
240
239
238
237
235
234
232
231
230
228
227
226
225
extraction of degraded hydrocarbons from culture media
trifluoroacetic acid
alpha-naphthylacetate- for staining white blood cells
staining plant material-toluidine blue
preparation of breed smears in food microbiology practical classes
di isopropylethylamine
experiments using stainless sted powder (maximum diameter)
lowry protein assay-folin and ciocalteus reagent
final year prosect-metabolic inhibition of streptomyles aureofaciens
final year prosect-metabolic inhibition of s.aureofaciens
final year prosect-metabolic inhibition of streptomyles
pthophosphoric acid
handling (weighting,diluting, dispensing)
handling (weighting,diluting, dispensing)
handling (weighting,diluting, dispensing)
diaminobenzydine (DAB)
use of commercial amylase preparations to saccharify starch
silica (silicon dioxide) in DNA and RNA extraction
determination of lactate by enzymatic oxidation of nicotinamide adenine
dinucleatide (NADH)
determionation of glutamine and ammonia concentration
enzyme linked immunobsorbent assay for the determination of
monoclonal antibody concentration in hybridoma culture supernatant
rose bengal chloramphenicol agar base
dichloran rose bengal chloramphenicol agar base
pristane
b-propridlactone
staining slides- teaching and research labs
periodic acid
hydrogen peroxide
citroclear for histological use as a safes alternative to xylene
protease fungal type XI
polyoxyethylene 20 cetyl ether
isophropy-bata-d-thiogalactoside (IPTG)
y-methacryloxypropyl-trimethoxysilane
mops free acid-buffer used in denaturing formamide gel for RNA
diethyl pyrocarbonate
adenosine tri phosphate
zinc sulphate
manganese sulphate 4-hydrate (manganous sulphate)
copper sulphate 5-hydrate (cupric sulphate)
magnesium sulphate-7-hydrate
dimethyldichlorosilane-used for coating plates fir PAGE electrophoresis
ampicillin-antibiotic selection of bacterial colonies
trizma-used to make buffery solution
temed
potassium acetate
224
222
221
220
219
218
217
214
213
210
209
208
206
205
204
203
199
198
195
192
191
188
184
183
180
177
171
168
167
165
163
162
160
156
154
153
145
144
143
141
140
138
136
135
134
133
ribonuclease C from human plasma-degradation of RNA
spermidine
dl-dithiothreitol-reducing agent
isoamyl alcohol (isopentyl alcohol 3-methyl-l-butanol)
deoxychloric acid
ethylenegrycol-bis-amino-ethyl ether-n-n-tetra acetic acid
making and using plant cell culture media
total carbohydrate determination by phenol sulphate method
phosphate assay
toluene
water activity determination using water activity meter
salvage of waste mercury from brokrn thermometers, manometers and
other mercury, containing apparatus
refluxing apporox molar lactic acid to hydrolyse anhydrides
production of nisin-inactivating enzymes by two bacillus species
enzyme-linked immunosorbent assay (eliza) for nisin
production of nisin-inactivating enzymes by two bacillus species
dichloromethane
bath culture of murine hybridoma
production, extraction and analysis of aflatoxins
removal of organic solvents by rotary evaporation
solvent extraction of mould metabolites
isolation of microorganisms for soil (fungi)
sodium ddodecyl sulphate (SDS)
boric acid
poly acryl gel electrophoresis
ammonia hydraxide solution
trietylamine (acetate)
guanidium thiocyanate-used for RNA extractions
methanol
ethanol-for pracipitation of DNA and RNA
oligonucleotide syntesiser
acetonitrine (methyl cyanida)
tetramethylethylenediamine (temed) used for pouring polyacrylamide gel
phorbol myristate acetate (PMA)
menoclonal antibody production in vitro
methylene blue for staining animal cells
preparation,use of concentrated sodium hydroxide solution
preparation of 0.2% ethylendiamine tetra acetic auo salt tetrasodium salt
ethanol 70%
preparation of cell cyto-protectant
production and use of bouins fluid
potassium tellurite, used in culture media
preparation of phenol solutions
nesslers reagent, test for ammonia production
nalidixic acid
iron sulphate 7-hydrate (ferrous sulphate)
133
132
131
129
128
127
125
124
123
121
120
119
118
118
117
116
115
114
112
111
110
109
108
107
106
105
103
102
101
100
99
98
96
95
94
93
92
91
90
89
88
87
86
85
84
83
82
xanthine used in culture media
fast blue BB salt solution in methoxyethanol
heamatoxylin
chloroform
oxalic acid
ninhydrin reagent for rapid hippurete lest
cleaning immersion oil of microscope sliced and objectives using xylene
tween 80 used in culture media
thiomersal
preparation of stock solution of thallous acetate to use
preparation of nitrate broth, using sodium nitrate
sodium iodate
preparation os selenite broth,
effects of selenium on tumour cells
sodium taurocholate,sodium desoxycholate
preservation of biological materials using sodium azide
metronidazole
use of methanol
griseofulvin
used of glutaraldehyde to fix red cells
freunds adjuvant
formaldehyde
use of calcium chloride
butanol, propanol
preparation of opacity standard using barium carbonate or barium chloride
api zyma reagent
amplotericin B
actidione (cycloheximide) in culture media
clocian acetic acid
production of stock lugols iodine
production of stock lactophenol
production of stock methylene blue
making up of stock aceid alcohol
production of stock acid ferric chloride
production of stock andrades indicator
production of stock benedicts solution
production od stock strong carbol fuchsin
production of crystal violet stain
production of stock borax methylene blue
preparation of stock kovacs reagent
production of stock acetone iodine
precipitation of nuclein acids
denaturing gel- to denature nucleic acid
E.D.T.A.(versene) buffering chelating agent
n,n dimethyl formamide-solvent for x-gel
fluorescent labelling of nucleic acids
dessication procedures in vacuum dryes
81
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79
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77
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75
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73
72
71
70
69
68
66
65
64
63
62
61
60
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58
56
54
53
52
49
48
47
46
45
44
43
42
41
40
36
35
34
33
31
30
decon-90 -soaking and washidg radioactive equipmant
zinc chloride-for proparation of butter for phosphatase reaction
ammonium persulphate-catalyst for polimorisation of acrylamide gel
acetic acid-fixing of sequencing gel
nonidet NP40- penaruring of nucleic acids for PCR and nucleotide
sequencing
tween 20 detergent denaturant in polimesare chain reaction (PCR)
preparation of RNA from virus and denaturant in minipreps
removal of contaminants from nucleic acid suspension
urea-denaturing agent in sequencing gel
triton-x-100
chcloroform-removal of mineral oil
chelation of mercuty ions after methyl mercury hydroxide clenaturation
formamide
large scale purification of plasmid by gradient centrifugation
proection of cells from damage when freezing and denaturing plasmids
prior to sequencing
acrylamide (powder) and solution up40%, bis-acrylamide used for gel
electrophoresis
precipitation of nuclein acids
cleaning sequencing plates
nitrate A solution
making of stock nitrate B solution
liquid partaffin
production of stock 40% potassium hydroxide
making up of 30% trichloroacetic acid
zinc dust
making of ONPG (o-nitrophenyl-B-D-galactophyranoside)
production of stock sudan black
chloran hydrate
sterilisation of plastic wave using formaldehyde
enzyme linked immunoabsorbent assays for the antibodies
barbituric acid
sodium barditone
copper chloride (cupric chloride)
iron chloride 6-hydrate (ferric chloride)
benethonuim chloride crystaline
conversion of ADP and AMP to ATP
perchlaic acid 60%
extraction of protein from bacterial cells
dinitrosalicylic acid reducing sugar assay
protein assay
extraction of amino acids from bacterial cells
ammonium assay
extraction of nucleotides from bacterial cells
glycerol assay
29
28
27
27
26
25
24
23
21
20
19
19
16
15
14
13
12
11
8
7
6
5
4
3
2
1
937(a)
840 (a - f)
834(b)
834(a)
83, 159
826(b)
820 (a to c)
819(b)
819(a)
818(b)
818(a)
effect of various agglutinins on normal and tumour cells, mitochondrial
proliferation assay
isolation of cells from normal tissues and tumour tissues
immunocytochemistry of normal and tumour tissues
freezing and preservation (storage) of normal tisues, tumour tissue and
cells, denaturing of plasmids
monoclonal antibody production in vivo
culture of normal and tumour cells
immunocytochemistry and immunohistochemistry of normal and tumour
tissues
immunoblotting, western blotting, of normal and tumour homogenates
immunoblotting, western blotting, of normal and tumour homogenates
ammonia solution 35%
handling and diluting concentrated acids: sulphuric acid, hydrochloric
acid, nitric acid
handling and diluting concentrated acids: sulphuric acid, hydrochloric acid
darkroom procedures, developing and fixing using hydroquinone
developers
darkroom procedures, developing and fixing using thiosulphate fixers
darkroom procedures, developing and fixing films and papers with acetic
acis stop baths
darkroom procedures, the preparation of blue slides using diazo process
surface sterilisation of plant material
fixation, preservation and micro examination of plant material
plasmid elimination by acridine orange in nutrient broth
preparation of cloramphenicol solution
production of p-nitrophenol
production of 4-methylumbelliferone-free acid by e. coli
tetrox (sodium hydroxide) used in the decontamination of autoclaved
reusable glassware
the making up of stock solution of sodium hypochlorite
labsol
hycolin
preparation of tween-85 expanded vanadium oxide
isolation and analysis of compounds from plant extracts
catalytic reaction of so2 to so3 (20 az 02), gc
catalytic reaction of so2 to so3 (20 az 02), gc, ozone generator
ethidium bromide-used for staining nuclei acids
synthesis of calix derivative
pot trial using arsenic contaminated soils (coshh assessment and atomic
absorption risk assessments)
dna extraction from mycobacterium tuberculosis complex organisms (see
above - this is the same assessment with a new researcher)
dna extraction from mycobacterium tuberculosis complex organisms
mycobacterium tuberculosis and tb complex organisms. sub culture (see
above - this is the same assessment with a new researcher)
mycobacterium tuberculosis and tb complex organisms. sub culture
797 (b)
73a
71, 176
70, 166
69, 170
66, 197
65, 270
65, 158
63, 212
60, 147
567ab,c,d
565a,b,c,d
562a,b,c,d
551d
551a,b,c
550a,b,c,d
510a,b
51, 215
51, 169
51, 150
51, 150
51, 142, 187
482c
482b
482a
460b
460a
459a
403, 403a
395a,b
377a
37, 38, 39
365a,b
336, 355
2a
296, 297
292, 310
288, 330
288, 329
288, 322
274, 278
274, 277
274, 276
274, 275
220, 270
Colormetric assay for fungal siderophore production in liquid culture using
the Chrome Azurol technique
urea as substrate in urease assay
chloroform extraction
used of mercaptoethanol in poly acrylomide gel electrophoresis
rormamide
dimethyl sulphoxide
SDS polyacrylamide gel-electroforesis, preparation and loading
acrylamide used as polymes for polyacrylamide gel
protein/carbohydrate precipitalion by means of addition of cold acetone
preparation of eliza substrate buffer, use of o-phenylenediamine
addition to bacteria cells
making solution for addition to soil
hydrolarbons as GC standards
yeast nitrogen base use in microbiological media
chlorophenolred-B-D-galactopyranoside, Na salt, oestradiol, rubidium
chloride
engine oil to be used as carbon source in mineral media
handling of human skin tissue
chemical sterilization of equipment and apparatus
formaldehyde solution
decontamination of sefty cabinets
decontamination of safety coabinets
preparation of cell fixative solution
handling of acanthamoeba castellanii (douglas)
observation and cultivation of microorganisms from plants,soil amd water
handling hazard group 2 micro-organisms
extraction beffer preparation, use sodium acetate
extraction buffer solution
cell culture assesment-elisa
pholloidin (tric-labelled)-fluoresence staining of acting filaments
baf fmt 30-used as an antifroam in fermentation
belousou-zhabotinsky reaction
schinder orcinol assay for rna
ethyleneglucol
antibiotic assay
teepol
reducing sugar estimation (nelsone somogii)
assay for peroxide/hydrogen peroxide
trypan blue for the determination of cell densities
2nd year virology practical classes
animal cell practical trypan blue
piperidine
hydroxybenzotriazole
ethanediol
acetic anhydride
isoamyl alcohol-use in preparation of extraction mixes
210, 273
102, 248
101, 161
434,436,437
309,350,358
193,195,284
preparation of formazin turbidium standards used to calibrate turbidium
meter
preparation of formazin stock solution for turbidily meter calibration
boric acid
boric acid
puritication of mini prop DNA by use of guanidinium thocyanate method
high performance liquid chromatography (HPLC) procedure
preparation of MTT solution for colorimetric assay of cell growth and
viability
use of phorbol myristate acetate (PMA) to induce COX-2 expression in
prostate cosncer cell lines
sodium hydroxide solution
sodium hydroxide (NAOH)
o-phenylenediamine dihydracholide
preparation of o-phenylenediamine substrate buffer for eliza assay
phenol extraction
mineral media
twee 80 used in culture media
use of thiomersal in eliza sobstrate buffer
isopropanol-precipitation of nuclein acids
isopropanol-precipitation of DNA and preparation of isopropanol saturated
with caesuim chloride
cycloheximide (actidione)
acetic acid
phospotase assay (soil)
nisin elisa-peroxidase conjugate
derivatisation of aflatoxin
168,173,181
71,114,187
373,374
369,370
352,345
351,359
348,357
347,354
346,353
266,340
114,279
RNAzol-b- extraction of RNA
thin layer chromatography procedures (TLC)
spiking of metalworking fluid emulsion with compand location
sbs 1-9 practical cell and molecular biology
n-hexadecane in culture medzia
handling of pyridine
conjugate syntesis-sodium periodate
antibiotic assay
use of thrombin to clean proteins
production of feeder cells for hybrid cell production
preparation of 0.1% crystal violet, 10% methanol solution for cell staining
210, 273
183a
183, 229
168, 405
162, 189
157b
156a, 172
145, 323
145, 186
137/147
137, 147
136, 173
126a,b,c,d,e
124, 232
123, 208, 249
107, 223
107, 179