BIOLOGY 30 Cell Anatomy, Cellular Processes, Scientific Process and Biological Scale REVIEW Name: ______________________________________________ Animal Cells Animal cells, unlike plant cell, do not have a regular shape. In fact, some animal cells, such as phagocytes, are able to alter their shape for various purposes (e.g., engulfment of foreign material). Animal cells differ from plant cells in other ways too: they lack a cell wall, and some of their structures and organelles are different. The diagram below shows the features of a typical animal cell. Some of these are common to plants. Further organelle descriptions are given on the page of plant cells. Plant Cells Plant cells are enclosed in a cellulose cell wall. The cell wall protects the cell, maintains its shape, and prevents excessive water uptake. It does not interfere with the passage of materials into and out of the cell. In the diagram below, descriptions have been provided for most of the cellular structures and organelles. Further descriptions are provided on the page on animal cells. Eukaryote Cells Cell Structures and Organelles 1. Nuclues 2. Rough endoplasmic reticulum 3. ribosome 4. centrioles 5. mitochondrion 6. cytoplasm 7. cell membrane 8. golgi apparatus 9. lysosomes 10. smooth endoplasmic reticulum 11. vacuole 12. cell wall Label the organelles depicted in the diagram. 1. Briefly describe the function of the following cell structures: a.) Cell membrane: regulates movement of material in and out of the cell b.) Cell wall: provides structure and protection (rigid) c.) Cytoplasm: maintains shape of cells, contains various solutes as well as contains the other organelles 1 d.) Nucleus: control centre, contains genetic material Cell Processes e.) Endoplasmic reticulum: transportation of materials f.) Mitochondria: Powerhouse of cells, cell respiration – glucose to ATP g.) Chloroplasts: converts solar E to strored chemical E in sugar via P.S h.) Golgi apparatus: stores, modifies, and packages materials for transport 1. For each of the processes listed below, state which organelles or structures are associated with that process (there may be more than one associated a.) Secretion Cell membrane b.) Respiration Cell membrane c.) Pinocytosis Cell membrane d.) Phagocytosis Cell membrane e.) Protein ribosomes synthesis 2. Define the term cell organelle: component of cell that carries out specific functions 3. Describe the role of the cytoskeleton in the cell: flexible structure that maintains shape, guides the movement of materials as well as holding the organelles in place. f.) Photosynthesis chloroplast g.) Cell division h.) Autolysis Centrioles, nucleus lysosome i.) Transport in / out of cell Cell membrane with a process): 2. Define the term metabolism: Active and Passive Transport Metabolism – The whole range of biochemical processes that occur within us (or any living organism). 4. Explain the role of centrioles in animal cell division: Form spindle fibres what attach to chromosomes which in turn pull the sister chromatids apart during cell division to opposite sides of the pole Active Transport –processes that requires energy by the cell Passive Transport – processes that do no require energy by the cell 2 b.) Distinguish between pinocytosis and phagocytosis: pino = cell drinking, not specific, gulps surrounding ECF Phago = cell eating, specific, larger molecules/ organisms 6. Name a type of human blood cell that uses phagocytosis in its functional role: White blood cells/ leucocytes 7. Name two gases that move into or out of our bodies by diffusion: CO2, O2 1. Briefly describe the energy requirements of passive and active transport: Passive = no E Active = E 2. Name a type of cell in the human body that requires an ion pump in order to function. Nerve cell 3. a.) Describe what happens in the process of exocytosis: Materials are transported out of the cell via vesicles that fuse with the cell membrane active transprort 4. b.) Name a secretory gland which has cells where exocytosis takes place. Pancreas, thymus, gonads 5. a.) Pinocytosis and phagocytosis are two forms of endocytosis. Describe the general process of endocytosis: materials transported into the cell via active transport 3 Biology 30: Basic Components to the Scientific Process 1. Problem Statement/ Objective: Formulate a cause and effect question Usually stated as: o What is the effect of the manipulated variable on the responding variable? o Example: What is the effect of the amount of sunlight on the growth of a plant? 2. Hypothesis/ Prediction: A prediction is simply answering the problem statement based on previous knowledge and experience (State what you think is going to happen). A hypothesis not only answers the problem statement but also provides a explanation for the provided answer (State what you think is going to happen and why). Hypothesis statements usually have the following format: o If….then…..because… o Example: If a plant is exposed to increased light, then it will experience more growth because plants use light in photosynthesis to make their own food. 4 A quick Exercise: For each of the following questions: identify the manipulated, responding, and controlled variables; provide a hypothesis; identify the control and experimental groups. 3. Experimental Design: A. Background Information –Write a 2 to 3 sentence statement that summarizes how the experiment will be carried out (a brief overview of what is to be done). A diagram may be used to illustrate how the equipment is set up B. Variables – Manipulated Variable – A condition that is deliberately changed by the experimenter (the one factor that will be changed Responding variable – a condition that changes in response to the to the change in the manipulated variable (the one factor that will be measured) Controlled Variables – conditions that are kept the same by the experimenter (provide a minimum of 3 controlled variables) Note: Variables in an experiment may also be independent or dependent variables. An independent variable is any variable that influences the effect being studied, but is not itself affected by the experimental conditions. Time is often an independent variable. The dependent variable is the same as the responding variable. What is the effect of the amount of Diet Coke consumed on the frequency of urination? 2. What is the effect of the amount of carbon dioxide concentration in the blood on breathing rate? Microscope Use Review Calculating Magnification Summary chart: Objective lens used Eyepiece Mag Object lens Mag Total Mag F.O.V Low Power Medium power High Power Calculations: Converting micrometers to millimetres: 1000 micrometers = 1 millimetre 1000 µm = 1 mm 1 µm = 0.001 mm Along with variables, often an experiment will have two parts for comparison: 1. The experimental group or part that tests the condition 2. The control group (commonly called the control) – conditions remain unchanged/ constant or do not experience the manipulated variable. Estimating Size of Object: (S.O.O) 1. Determine the field of view. (F.O.V.) 2. Determine the fit number (fit#) (How many times does the object fit across the viewing area?) 3. S.O.O. = F.O.V. calc. on the back of your drawing. fit# EXAMPLE: This worm was found under medium power and fit across the screen twice. Calculate the actual size of the worm. Step 1. F.O.V. = 1.6 mm Step 2. fit# = 2 Example: What is the effect of the amount of light on the growth of a tomato plant? Control Group: Plant grown in light 1. Experimental Group: Plant grown in dark Step 3. S.O.O. = F.O.V. fit# = 0.8 mm 5 = 1.6 mm 2 MAKING A BIOLOGICAL DIAGRAM Example: Calculate S.O.O and Scale for the cell labelled “a” in the diagram below using the provided information: All diagrams must follow the following rules: 1. Use unlined, white paper. 2. Draw only one cell (one image) - draw only what you can see!! (Some slides may have several cells showing, pick only one and draw it!!) 3. Draw the image approximately half a page in size using clear, unbroken lines. 4. Add details to your diagram using only the OUTLINE of the structure. ***Do NOT shade or colour your diagrams!! Stains used in slide preparation alter the natural colour. 5. Labels are drawn using straight horizontal lines and written in lower case (no capital letters) to the right of the diagram!! 6. Measure, with a ruler, and record on the diagram the length of the diagram in millimetres. 7. In the lower right hand corner of your diagram include: 1. Your name 2. Subject of drawing 3. Power Magnification of microscope 4. Size of the object. 5. Scale of drawing 8. On back page of drawing include the following information: 1. Power Magnification of microscope 2. F. O. V. 3. Fit number 4. Calculation of size of object 5. Scale Calculation Onion Cell Viewed under medium power magnification a CALCULATING SCALE OF YOUR DRAWING 300 cm To calculate the scale of your drawing, use the following formula: SCALE = S.O.D = Size of Drawing S.O.O Size of Object Student Biological Drawing Note: Using a ruler, measure the size of your drawing (S.O.D) in millimetres. NOTE: Always measure along the longest span. Put this on your drawing. 6