Name____________________________________ Course:_______________________ Choose the best answer for the following questions, and then tell why the answer you choose is correct. 1. Glycolysis leads to the production of two: a. Lactic acid, NADH, ATP d. Pyruvate, NADH, ATP b. Pyruvate, NAD+, ATP e. FADH2, Pyruvate, NAD+ c. CO2 , Pyruvate, NAD+ _______________________________________________________________ _______________________________________________________________ 2. During metabolism WHICH reaction proceeds in the absence of oxygen? a. Glycolysis and ETS c. Glycolysis and TCA cycle b. Fermentation and the ETS d. Glycolysis and Fermentation _______________________________________________________________ _______________________________________________________________ 3. Which is true of the intermediate step of metabolism? a. CO2 and ATP are released during the process b. A multienzyme complex removes a carboxyl group, transfers electrons to NAD+, and attaches a coenzyme. c. NAD+ is rejuvenated so glycolysis can continue d. Lactate is produced to rejuvenate free NAD+ _______________________________________________________________ _______________________________________________________________ 4. Which is the most important function of Glycolysis? a. Production of NADH b. Modification of Glucose c. The production of ATP d. The production of CO2 ______________________________________________________________________________________________ ______________________________________________________________________________________________ 5. In the Chemiosmotic mechanism, a. ATP production results from a proton gradient established by the electron transport chain. b. The difference in pH between the intermembrane space and the cytosol drives the formation of ATP. c. The energy released by the reduction and subsequent oxidation of components of the electron transport chain is transferred as a phosphate to ADP. d. The production of water in the matrix by the reduction of oxygen leads to a net flow of water out of the mitochondrion. ______________________________________________________________________________________________ ______________________________________________________________________________________________ 6. The main function of the TCA cycle is accomplished between which stage? a. Oxaloactetate and citrate c. Citrate and Succinate b. Isocitrate and Succinate d. Succinyl CoA and Malate ______________________________________________________________________________________________ ______________________________________________________________________________________________ 7. Which enzyme of the TCA cycle has a dual role with the ETS and is located in the inner membrane of the mitochondrion: a. Pyruvate dehydrogenase c. Fructose dehydrogenase b. NADH Dehydrogenase d. Cytochrome oxidase c. Succinate Dehydrogenase ______________________________________________________________________________________________ ______________________________________________________________________________________________ 8. Fatty acids inter metabolism after they have been beta oxidized to form: a. Carbon dioxide c. Glucose b. Acetyl groups d. H2CO3 ______________________________________________________________________________________________ ______________________________________________________________________________________________ 9. The only usable ATP is produced here in the TCA cycle? a. Between Malate and Fumerate b. Between Succinate and Fumerate c. Between Succinyl Co-A and Succinate d. Between Succinate and Fumerate ______________________________________________________________________________________________ ______________________________________________________________________________________________ 10. What is the terminal electron acceptor during mitochondrial respiration? a. Water c. Hydrogen b. Oxygen d. NADH ______________________________________________________________________________________________ ______________________________________________________________________________________________ 11. As a result of glycolysis, pyruvate oxidation and the citric acid cycle, only a small portion of the energy of glucose has been converted to ATP. At this point, the majority of the usable energy is contained in: a. acetyl coenzyme A b. Pyruvate c. NADH and FADH2 d. Water ______________________________________________________________________________________________ _____________________________________________________________________________________________ 12. ATPs are synthesized in glycolysis and the TCA cycle by: a. Oxidative Phosphorylation c. Photophosphorylation b. Substrate-level Phosphorylation d. Anabolism ______________________________________________________________________________________________ _____________________________________________________________________________________________ 13. A product derived from pyruvate crosses the inner mitochondrion membrane by which of the following methods? a. Diffusion c. Active Transport b. Facilitated transport d. All of the above ______________________________________________________________________________________________ _____________________________________________________________________________________________ 14. Suppose the hexokinase activity was destroyed in the cell. Which of the following will occur? a. The cell will continue to produce ATP through the ETS b. The cell will continue to produce ATP in the citric acid cycle c. The cell will continue to produce ATP through Fermentation d. The cell will stop producing ATP ______________________________________________________________________________________________ _____________________________________________________________________________________________ 15. Dinitrophenol was once used as a component of diet pills for weight loss however, users were dying due to its consumption because the drug cause the inner mitochondrion membrane to become highly permeable which prevented ATP synthesis. How? a. After coenzymes dropped off electrons, they diffused into the intermembrane space from the matrix b. Electrons diffused from the matrix to the intermembrane space c. Protons diffused from the intermembrane space to the matrix, bypassing ATPsynthase d. Pyruvate is not allowed to enter the matrix ______________________________________________________________________________________________ _____________________________________________________________________________________________ 16. Which of the following least support the contention that the mitochondrion is a descendant of prokaryotes? a. They have porins in their outer membranes b. They have a diameter of .2 microns c. They swim freely in the eukaryotic cells d. They have circular chromosomes and 80s ribosomes ______________________________________________________________________________________________ _____________________________________________________________________________________________ 17. The light reactions of photosynthesis occurs: a. The stroma c. The matrix b. The thylakoid membrane d. The stoma ______________________________________________________________________________________________ _____________________________________________________________________________________________ 18. Which molecule binds to carbon dioxide to form a six-carbon sugar in the Calvin Benson Cycle? a. Ribulose Bisphosphate c. Glyceraldehyde-3-phosphate b. Citrate d. Phosphoglyceraldehyde ______________________________________________________________________________________________ _____________________________________________________________________________________________ 19. The Calvin-Benson Cycle occurs: a. The stroma c. The matrix b. The thylakoid membrane d. The stoma ______________________________________________________________________________________________ _____________________________________________________________________________________________ 20. Which is not a product of Photosystem II? a. NADPH c. O2 b. ATP d. All are products of Photosystem II ______________________________________________________________________________________________ _____________________________________________________________________________________________ 21. CO2 required for carbon fixation, gets into the Calvin-Benson cycle through which structure of the leaf? a. The stroma c. The matrix b. The thylakoid membrane d. The stroma ______________________________________________________________________________________________ _____________________________________________________________________________________________ 22. How many pairs of electrons are removed in the TCA cycle? a. 4 b. 5 c. 6 d. 12 ______________________________________________________________________________________________ _____________________________________________________________________________________________ 23. The reason that anaerobes can produce a maximum of 38 ATPs from a molecule of glucose while aerobes can only produce 36 is because: a. Anaerobes do not lose two ATPs in glycolysis b. Anaerobes do not have an ETS c. Anaerobes do not undergo oxidative phosphorylation d. Anaerobes produces an extra FADH2 during the TCA cycle ______________________________________________________________________________________________ _____________________________________________________________________________________________ 24. During glycolysis, ATP are synthesized by: a. substrate-level phosphorylation b. oxidative phosphorylation c. photophosphorylation d. all of the above ______________________________________________________________________________________________ _____________________________________________________________________________________________ 25. Cyanide is a poison that blocks the passage of electrons along the electron transport chain. Which of the following is a metabolic effect of this poison? a. The pH of the intermembrane space is much lower than normal b. Electrons are passed directly to oxygen, causing cells to explode. c. Alcohol would build up in the cells d. NADH supplies would be exhausted, and ATP synthesis would cease. e. No proton gradient would be produced, and ATP synthesis would cease. ______________________________________________________________________________________________ _____________________________________________________________________________________________