(The front cover will look something like this) The University of Sydney Faculty of Veterinary Science UNIT OF STUDY NAME VETS XXXX example of picture for front cover Unit of Study Handbook 2005 Unit of Study Coordinator Dr XXXX XXXXXXX This handbook belongs to _________________________ (inside front cover) UNIT OF STUDY DESCRIPTION (this should be the same as in the Faculty handbook, excluding information about assessment and textbooks) for example:VETS 1006 Cell Biology 1A 4 credit points Dr Paul Sheehy Offered: February. Assumed knowledge: see UAC handbook. Classes: 37 lectures, 8 hours of practical classes and 2 hours of tutorials. This unit will introduce the student to the biology of the cell. Topics include cell structure and molecular biology. The cell structure component includes a description of cell membranes and organelles. The molecular biology component includes a discussion of enzyme structure and metabolic pathways. Clinical material is used to illustrate normal structure and function. Copyright This unit of study handbook is copyright of the various source textbooks and the contributing staff members of the Faculty of Veterinary Science, University of Sydney. The handbook and associated printed teaching materials, is distributed to enrolled students on a non-profit cost recovery basis. Where illustrative material in the manual is taken from standard textbooks it is on the assumption of fair usage for teaching and is for non-commercial purposes. Warning: Commercial use of any material in this teaching manual is an infringement of copyright. TABLE OF CONTENTS For example Page nos will be inserted later 1. GENERAL COURSE INFORMATION .......................................................x 1.1 Timetables...................................................................................x 1.2 Staff .............................................................................................x 1.3 Learning aims/Outcomes ........................................................... 7 1.4 Graduate Attributes .................................................................. 11 1.5 Textbook List ............................................................................ 12 1.6 Library Resources/VEIN ........................................................... 13 1.7 Online Learning ........................................................................ 13 1.8 Assessment.............................................................................. 15 1.9 Safety Notes/Responsibilities ................................................... 20 1.10 University Policies and Guidelines ........................................... 20 1.11 Faculty Guide for Assignments ................................................ 21 1.12 Improving the Course: Feedback from Students ...................... 27 1.13 University Learning Centre ....................................................... 28 the next three sections are optional and will vary according to the content of your Unit of Study 2. PRACTICAL CLASSES & TUTORIALS for example ............................ 29 2.1 Practical Class 1 - title ............................................................... 34 2.2 Practical Class 2 - title ............................................................... 34 2.3 Practical Class 3 - title ............................................................... 34 2.4 Practical Class 4 - title ............................................................... 34 2.4 Tutorial 1 – topic ............................................................................ 2.5 Tutorial 2 – topic ............................................................................ 3. READINGS (for example).......................................................................... 47 Journals articles/reviews ................................................................. 48 Case histories...................................................................................... 4. LECTURE NOTES for example) .............................................................. 61 Lecture topic 1 ................................................................................. 62 Lecture topic 2 ................................................................................. 88 Lecture topic 3 ................................................................................. 90 5. APPENDIX for example ............................................................................ 157 Data sheets ..................................................................................... 62 Statistical tables .............................................................................. 88 Past exam papers ........................................................................... 90 1 1.1 GENERAL COURSE INFORMATION TIMETABLE, Unit of Study name, VETS xxxx The following is a good example of an easy to read timetable. Please create your timetables in TABLE format as tab stops are too difficult to alter. WEEKLY PROGRAM LE GE N D L Lecture P Practical Class Week Class No. Lecturer 1 2 VSCC CARNE VAL GL4 Date Veterinary Science Conference Centre Carne Lecture Theatre, RMG Gunn Bld Veterinary Anatomy Laboratory Gunn Level 4 Lab A PS IT Time Venue Lecture/Prac/Tutorial Paul Sheehy Imke Tammen CB L1 CB L2 CB L3 CB L4 CB L5 CB L6 CB L7 CB L8 Prac CB P1 Mon 8 Mar Mon 8 Mar Mon 8 Mar Tue 9 Mar Tue 9 Mar Tue 9 Mar Wed 10 Mar Thur 11 Mar Thur 11 Mar 9am 10am 11am 10am 11am 12noon 9am 10am 11-1pm CARNE CARNE CARNE CARNE CARNE CARNE VSCC VSCC VAL General Introduction to Cell Biology PS/IT Small Molecules, Energy and Biosynth 1 PS Cell Types and Morphology IT Small Molecules, Energy and Biosynth 2 PS Plasmalemma IT Small Molecules, Energy and Biosynth 3 PS Small Molecules, Energy and Biosynth 4 PS Cell Organelles 1 IT Microscopy PS/IT 2 Mon 15 Mar Mon 15 Mar Mon 15 Mar Tue 16 Mar Tue 16 Mar Tue 16 Mar 9am 10am 11am 10am 11am 12noon CARNE CARNE CARNE CARNE CARNE CARNE Cell Organelles 2 Macromolecules 1 Cell Organelles 3 Macromolecules 2 Cell Inclusions Macromolecules 3 CB L9 CB L10 CB L11 CB L12 CB L13 CB L14 D:\726851291.doc IT PS IT PS IT PS CB L15 Wed 17 Mar 9am VSCC CB L16 Thur 18 Mar 10am VSCC Prac CB P2 Thur 18 Mar 11-1pm VAL 1.2 Macromolecules 4 Enzyme Catalysis Tissue Preparation PS PS PS/IT STAFF For example: please include photos of staff and a description of their research interests Five entries to each page. Photo (50KB max Name file size, image size 3 x 3cm) Dr John Citizen Dr Jack Horse-doctor details Research interests Unit of Study coordinator Room 101, Gunn Bldg Tel: xxxx xxxx Email: johnc@xxxxxxx Room 202, Macmaster Bldg Tel: xxxx xxxx Cat behaviour Paragraph of words 20 Horse behaviour Include support staff names and contact details, if relevant. D:\726851291.doc 3 1.3 LEARNING AIMS/OBJECTIVES/OUTCOMES Aims 1. The Unit of Study Aims situate the Unit of Study in relation to the course as a whole. The key points should be The relationship of this Unit to previous Units of Study The key focus of this Unit of Study The relationship to subsequent Units of Study Learning Outcomes 2. The Unit of Study Learning Outcomes describe what you hope the students to attain by the end of the Unit. “By the end of this Unit of Study, students will be able to……” here are some examples Describe the main structural features of a nucleated cell. Explain the role of these structures in the normal processes of cells. Describe, in general terms, how these structures are made visible in special preparations of tissues and how they are used in identification of different types of cells List the elements that are essential for life, and give examples of elements that are toxic and some which are used in therapy Describe the basic form of the communication that takes place in a veterinary consultation and identify those elements that are necessary for a successful outcome Explain, using examples of common conditions, how perturbations of normal biochemical processes can give rise to metabolic disorders, and how knowledge of the biochemical pathways can be used in diagnosis of these disorders 4 D:\726851291.doc GRADUATE ATTRIBUTES 1.4 Notes: The Faculty of Veterinary has its own list of generic attributes of the Veterinary graduate. Those that are specifically addressed in your Unit of Study might be included here. It shouldn’t be necessary be include the entire list in each book. Faculty of Veterinary Science: Revised Veterinary Graduate Attributes REVISED DRAFT 10/2/04 1 2 Research and Inquiry. Graduates of the Faculty of Veterinary Science will be able to create new knowledge and understanding through the process of research and inquiry. A. Apply an understanding of normal and abnormal animal structure, function and behaviour for diagnosis, management and prevention of animal disease B. Identify, define and analyse problems affecting animal health and production C. Collect and use the best available evidence to diagnose, prevent, cure or manage animal health problems D. Maintain effective skills for identifying and responding to emerging animal diseases and issues E. Engage in research-based practice, using critical judgment and creativity F. Collaborate in the generation, application and dissemination of new knowledge to benefit animal health and welfare G. Have an informed respect for the principles, methods, standards, values and boundaries of their discipline and the capacity to contribute to and question these H. Critically evaluate existing understandings and reflect on the limitations of their own knowledge Information Literacy. Graduates of the Faculty of Veterinary Science will be able to use information effectively in a range of contexts. A. Identify acquire, store, retrieve, interpret, critically evaluate and use scientific, clinical and other relevant information in print and electronic sources D:\726851291.doc 5 3 4 B. Prepare a scientific or clinical report in a form suitable for publication and presentation C. Use networked services and information technology efficiently in research, professional development and practice management D. Investigate emerging technologies and determine their relevance to the profession Personal and Intellectual Autonomy. Graduates of the Faculty of Veterinary Science will be able to work independently and sustainably, in a way that is informed by openness, curiosity and a desire to meet new challenges. A. Make independent, informed professional decisions and implement them in managing animal health B. Evaluate one’s own abilities, identify deficiencies and commit to continuing professional learning C. Identify situations where additional expertise is necessary, seek specialist services and refer patients professionally D. Prepare a curriculum vitae, identify work opportunities, apply, present at interview and negotiate an employment contract E. Have the capability and commitment to initiate and respond to change F. Develop a capacity for managing one’s own personal, physical, emotional and social needs to sustain satisfaction and contribution to profession Ethical, Social and Professional Understanding. Graduates of the Faculty of Veterinary Science will hold personal values and beliefs consistent with their role as responsible members of local, national, international and professional communities. A. Practice veterinary science professionally: with primary consideration to the welfare of the animals in care to uphold the ethical standards and legal requirements of the profession to meet the health and safety needs of oneself, colleagues, ancillary staff, clients and the community with colleagues, ancillary staff, clients and the public with respect and without discrimination 6 B. Apply the principles of animal welfare to the humane management and euthanasia of animals C. Ensure animal products are free of chemical residues and other contaminants D:\726851291.doc 5 D. Protect the natural environment, maintain biodiversity and conserve endangered species E. Ensure sustainability of agricultural activities through the practice of veterinary science on livestock that considers the economic and social needs of farms and the livestock industries F. Practice veterinary science in Australia with awareness of the need to keep Australia free of non-endemic diseases and ensure exported animals are free of disease G. Practice veterinary science in countries other than Australia with awareness of local differences Communication. Graduates of the Faculty of Veterinary Science will recognise and value communication as a tool for negotiating and creating new understanding, interacting with others, and furthering their own learning. A. Communicate effectively in English, both orally and in writing, with a variety of recipients and audiences and using a variety of media B. Consult effectively, eliciting the history and clinical signs from clients systematically and with sensitivity C. Prepare and maintain records of clients and animals and the results of veterinary interventions and procedures promptly, accurately and concisely D. Work effectively as individuals, partners and as members of a team E. Contribute or lead a group in cooperative problem-solving F. Communicate with colleagues, ancillary staff, clients and the public with patience, empathy and compassion D:\726851291.doc 7 1.5 TEXTBOOK LIST include both compulsory and recommended textbooks, readings and journal articles. 1.6 LIBRARY RESOURCES/VEIN/ONLINE LEARN ING University Library Services While you are studying at the University of Sydney you have free access to the largest academic library in the Southern Hemisphere. Badham and Camden Libraries are the specialist libraries for veterinary science. The Library is happy to transfer items between campuses for you. Resources available include books, journals, videos, research databases, information skills training and access to qualified information specialists. Many of the resources on your reading lists will be available in the Reserve Collection at Badham or Camden. You will find that many of the journal articles and book chapters are available in electronic format. This allows you to access the material anywhere on campus and from locations off campus. Access to databases and some ejournals is also available off campus, please see: http://www.library.usyd.edu.au/databases/wam.html for more information. Check these entries Your Faculty Liaison Librarian at Camperdown Campus is: Sarah Graham Your Faculty Liaison Librarian at Camden Campus is: Karen Black Badham Library Ground Floor, Badham Building, A16 Science Road, Camperdown Campus, University of Sydney, NSW, 2006 Tel: 9351 7319 Fax: 9351 3852 Email: s.graham@library.usyd.edu.au Camden Library University of Sydney Farms, C15 Werombi Road, Camden, NSW, 2570 Tel: 9351 1627 Fax: 46 55 6719 Email: k.black@library.usyd.edu.au VEIN VEIN, or the Veterinary Education and Information Network, is a web portal maintained by the University Library and supported by the Faculty and the Veterinary Science Foundation. It provides relevant, quality information to assist you in your studies. The website is: http://vein.library.usyd.edu.au/ VEIN will allow you to easily access the facilities of the University Library, including catalogues, research databases and high quality websites. The ‘What's New?’ page advertises events on and off campus - please let the Library know if you have a Vet event coming up so it can be listed. The ‘Links’ pages on VEIN gather together resources by area of study, species and disease as well as providing information on personal and professional 8 D:\726851291.doc development, the use of technology and organisations. Please use VEIN and contribute to the development of the Website by using the ‘suggest a site function’ on each page or by contacting your Faculty Liaison Librarian. Referencing and Bibliographies The Faculty recommends the use of the Harvard referencing system, as modified by Flinders University. Please see the Flinders University library website: http://www.lib.flinders.edu.au/resources/sub/healthsci/referencing/referencelist.html ONLINE LEARNING 1.7 Online learning This Unit of Study has some elements available online. What follows is a brief introduction to studying in this environment, including what equipment you will need to study online, how to find your unit of study, how to make the most of studying in this environment and where to go to find help. What Do You Need To study online you need regular access to a computer with internet connection either at home or at the University or a local Internet Café. If you are using your home computer the minimum requirements are: How PC486 or better running Windows 95, 98 or 2000 Mac 68030 or better running at least OS 7.5 At least 16Mb RAM and 20Mb free disk space Modem 28k bps or faster A web browser IE4 or above or Netscape 4.** (not Netscape 6) to Get In Open a browser (either Netscape or Internet Explorer) and go the USydNet site: http://intranet.usyd.edu.au, click on Units of Study – WebCT and you will be taken to your online learning site (if at home you should probably bookmark this site). You will be asked for a Username and Password these will be the Unikey (Extro account) Username and Password that you were sent with your confirmation of enrolment. Once you have entered appropriate Username & Password you will enter the online area for your Unit of Study. What to Expect The web is a new learning environment for most people and it’s probably a good idea to have a look around when you first arrive. Explore your online Unit of Study and check out what information and tools are available. Remember this is a new medium and will require some adjustment to use effectively – it is not a book and it is not a classroom though it has elements of both be prepared to play around until you feel comfortable and don’t be scared of making mistakes. D:\726851291.doc 9 The tool being used to support this learning environment is called WebCT if you wish to know more about it there is a CD available from your lecturer or the IT helpdesk. When using web based communication tools it is worth remembering there are real people on the other end. As there are no accompanying physical expressions or vocal cues to enhance communication it is worth making extra effort to ensure our messages are clear and easily understood. Inappropriate and discriminatory comments, which would not be tolerated in the physical classroom, are equally unacceptable in the online environment. Where to Get Help If you are in a WebCT session and you encounter problems the WebCT Help button will give you quick, simple answers to most straight forward problems. If you are having trouble logging in or are faced with more complicated WebCT related problems email the ITS Help Desk at http://helpdesk.usyd.edu.au or by ringing 9351 6000. If you are having trouble getting through to your online learning site from home you may need to contact you own Internet Service Provider or the ITS Help Desk. If you have queries about the subject matter within the Unit of Study you should contact the academic(s) in charge. 10 D:\726851291.doc 1.8 ASSESSMENT Use the University policy “Principles http://www.usyd.edu.au/su/planning/policy/acad/Assess_Exam.pdfl as a general guide for this section. of Assessment” What are the grade descriptors, assessment components, due dates and weighting for assessment tasks in your Unit of Study? Give details of the requirements and format (essay, report, spoken presentation, etc) for each type of assessment using examples appropriate to your discipline. For due dates, use a non-dated format , for example “due on the second Friday of the semester, that is, the end of the second week of the semester”. Students should be reminded to submit assignments with the assignment cover sheet attached, available form the Faculty Office and on the WebCT site for the Unit of Study. Summative Assessment A description of the grade descriptors; e.g. what will students have to achieve in order to get a Credit, Distinction etc? Timing of assessments, nature, value of each assessment, etc. For example Task No 1. 2. 3. Component (eg Due Date essay, exam, etc) Essay 1000 words End of week 3 by 5 pm Friday Project presentation End of week 7 (oral) 15 minutes Written examination Examination period Weight 25% 25% 50% Formative Assessment The most important thing here is to explain how formative assessment will be given – in what form, on what pieces of work and when. A Faculty guide for assignments is available online through the WebCT Year sites If WebCT on other online resources are used for assessment, they should be included here. D:\726851291.doc 11 1.9 SAFETY NOTES/ RESPONSIBILITIES When handling animals in practical classes, students should be mindful of the ways in which animals defend themselves. For the safety of personnel and animals, students should seek advice from demonstrators if they are unsure how to handle or manipulate an animal. This section should include specific instructions relating to the safe working procedures in practical classes and other sessions in which the students will be required to take particular precautions with respect to procedures, reagents, equipment and the like. Notes on quarantine procedures should be included here. Some examples There is to be no smoking, eating or drinking in the laboratory at any time. Laboratory coats and shoes must be worn at all times Broken glass, needles etc should be placed in the “SHARPS” bin. They must not be placed in normal rubbish bins. Handle all cultures at all times with extreme care. All organisms used in classes are potential pathogens. When anaesthetising surgical patients scrub suits and protective shoes must be worn. 12 D:\726851291.doc RESOURCES – Faculty and University Policies & 1.10 Guidelines The University of Sydney policies outline the roles and responsibilities of the University to its students themselves. Administrative requirements can be found in the Veterinary Faculty handbook and other relevant requirements can be accessed via the Central Policy Index page at: http://www.usyd.edu.au/policy/ Policies relevant to veterinary students can be found on the Veterinary Faculty home page at http://www.vetsci.edu.au/ and follow the prompts to ENROLLED STUDENTS, then “Information for current students”. Some important resources include:Faculty Assignment and Referencing Guide Policy on Academic Honesty Policy on Special Consideration Plagiarism Policy Plagiarism or copying in any form in assignment tasks or quizzes is not tolerated. A mark of zero will be awarded when plagiarism occurs and the matter will be referred to the Associate Dean for Teaching and Learning, Faculty of Veterinary Science for further disciplinary action. Plagiarism can be broadly defined as knowingly presenting another person’s ideas, findings or written work as one’s own by copying or reproducing them without due acknowledgement of the source. Plagiarism may involve copying the work of another student, or it may involve paraphrasing or copying a published author’s text or argument without giving a reference. At its worst, plagiarism is theft. Students should be familiar with the following policies: Student plagiarism: Coursework Policy and Procedure: http://www.usyd.edu.au/senate/policies/Plagiarism.pdf and Academic Honesty in Coursework (Plagiarism): http://www.usyd.edu.au/ab/policies/Academic_Honesty_Cwk.pdf D:\726851291.doc 13 1.11 Faculty Guide for Assignments REFERENCING Correct use of citations and bibliographies is an important aspect of scientific writing and is not difficult if you follow these guidelines. The correct style is described on the following pages. The most common flaws Failure to cite source of information in body of text If a statement is made it must be supported by a reference, unless it is a well-accepted scientific fact. Incorrect & incomplete in-text citations Copying all the details of the reference (i.e. title, journal, as well as authors and year) into the body of the text is incorrect. Similarly, incomplete citations are incorrect, eg. "Smith and Jones found possums…" as the year of publication must be included. Inclusion/exclusion of references from the bibliography A bibliography is not a list of papers which you read in preparing the essay, or which you would recommend reading. It is a complete and comprehensive list of all the articles/books, which were cited in the essay, including those used in figures or diagrams. It should not include references that were not cited. Incorrect citations The bibliography should be prepared last, after the essay is complete, so references are not missed. The list should be carefully proof-read and cross-checked with the text. A bibliography with many errors makes your work look sloppy. Citation of References The faculty referencing style is based on the Author-date (Harvard) referencing system used by School of Nursing & Midwifery at Flinders University For more information on the style see: http://www.lib.flinders.edu.au/resources/sub/healthsci/referencing/contents.html There are two methods of quoting, paraphrasing and referring to another author’s idea in your essay: “Stone (1990, p.34) showed that...” or “Rats excreted 75% of the ingested toxin”. (Stone 1990, pp. 25-26) When two or three authors are involved, cite both each time they appear, thus: 14 D:\726851291.doc “Rose and Hodgson (2000, p. 10)...” “Scott, Miller and Griffin (2001, pp. 191-196)...” One study suggests (Scott, Miller & Griffin, 2001, pp. 191-196)...” When four or more authors authored the article, mention the first author listed, followed by the abbreviation et al. (meaning and others): “Meehan et al. (2003) assumed the act of foraging was a” (et al. = and others) When there are no identifiable authors: One patient education leaflet (Loss of your pet 2001) states that The Bibliography (List of references at the end of the essay) All forms of reference described below are arranged alphabetically according to authors’ names, eg: Allen, WJ (1974).... Allen, WJ (1976).... Allen, WJ Baker, AB & Smith, DN (1973)... Allen, WJ Carter, CW & Brown, JL (1971)… Where more than one paper is published in one year by an author or group of authors, they are listed chronologically thus: Heywood, LH & Wood, AKW (1985a)... Heywood, LH & Wood, AKW (1985b)... The forms used for different types of references are given below. Journals Cite references thus: Hurn, SD, & Edwards, GA 2003, ‘Treatment of fractures of the mandible and maxilla by mini titanium plate fixation systems in dogs and cats’ Australian Veterinary Journal, vol. 81, no. 11, pp. 666-670. Note: All author’s names and initials are given The full article title is given The name of the journal is not abbreviated The first and last page numbers of papers are given. Books Normal texts, i.e where the author(s) are responsible for all the content, eg: Blood, DC & Henderson, JA 1974, Veterinary Medicine, 4th edn, Lea & Febiger, Philadelphia. D:\726851291.doc 15 A chapter in an edited work (i.e. multi-author book), eg: Watson, ADJ 1977, ‘Anti-microbial therapy’, in Current Veterinary Therapy, ed RW Kirk, Lea and Febiger, Philadelphia. Reports and Newspaper articles A report sponsored by an organisation or institution: NSW Department of Agriculture 1980 Annual Report, Government Printer, Sydney A newspaper article where no author is listed: ‘Hazardous bacteria related to anthrax’ 2005, The Courier-Mail, 2 June, p.3. Internet Material on the Internet can be used as reference material for your essays and project reports. Such material takes many forms. It can appear as a publication in a refereed or unrefereed electronic journal, or as a general article. You should be aware of the nature of the publication being used, and its intellectual authority. All material on the Internet is copyright. You should acknowledge the source of any information used in your essay, and Internet publications must be listed in your bibliography. An electronic journal article in a full text database: Bradshaw, JSW, McPehrson, JA, Casey, RA & Larter IS 2002, ‘Aetiology of separation-related behaviour in domestic dogs’, Veterinary Record, vol. 151, viewed 2 May 2005, (IngentaConnect). A web site: Royal Society for the Prevention of Animals 2004, Million Paws Walk, viewed 30 April 2005, <http://www.rspca.org.au/news_info/events.htm>. A web site with no identifiable author: The Pig Site 2005, viewed 2 June 2005, < http://www.thepigsite.com/>. 16 D:\726851291.doc IMPROVING THE COURSE – FEEDBACK FROM 1.12 STUDENTS We welcome feedback on this Unit of Study and thank you for taking the time to offer constructive written feedback at the end of the semester A number of avenues for student feedback might be described, and how this feedback might be used to improve the course. Examples of improvements to the Course arising from student feedback should be included here. 1.12 UNIVERSITY LEARNING CENTRE http://www.usyd.edu.au/stuserv/academic_support/learning_centre/ The Learning Centre offers a wide range of workshops and other activities for students to help develop the learning and language skills needed for academic study. The Centre’s workshops are available free of charge to all enrolled students of the University throughout the calendar year. You can choose to participate in a range of workshops, varying in length from 3 to 12 hours, some of them being repeated many times throughout the year. During semester times, workshops usually meet once a week for 2 or 3 hours per session, over several weeks. During vacation, workshops usually meet over 1 to 4 days. The purpose of the workshops is both to teach particular skills and to provide an opportunity for practising those skills in a systematic way. There are also independent learning resources including some specially designed resources for practising reading, writing, speaking and listening skills. Some examples of courses offered are: Essay and assignment writing Oral communication skills Studying at university Conducting research For information and registration for any course contact the Centre: tel 9351 3853. Location: new Education building, level 7,room 722, A35, next to Manning House. D:\726851291.doc 17 2 PRACTICAL CLASSES & TUTORIALS Some examples follow 18 D:\726851291.doc PRACTICAL CLASS 1 2.1 Some examples: Histology Practical Class 1 BRAIN AND SPINAL CORD Videomicroscopy Exercise 1 This exercise will familiarise you with the microscopic structure of the cerebrum. HV 20.1 This H&E stained tissue section was taken from the cerebrum of a dog. Hold the slide up to the light and against a white paper background. Note that the gyri and sulci are dorsal and that the straight edge of the tissue is ventral. IDENTIFY: gyri sulci tracts of white matter corpus callosum eaudate nucleus Scan the slide using the 4x objective. IDENTIFY: tissue folding artefact lines gyri sulci pia mater Scan the slide using the 10x objective. D:\726851291.doc 19 IDENTIFY: the relatively cell free molecular layer adjacent the pia mate pyramidal cells stellate cells large pyramidal cells fusiform cells nerve fibres of the white matter oligodendrocytes in the white matter Using the 40x objective. Examine each of the cell types observed in the 10x objective scan. Note: The cerebral cortex is described as having 6 layers: • Molecular. Adjacent pia mater. Relatively cell body free. • External granular layer. Small neurons • External pyramidal layer. Medium to large pyramidal cells • Internal granular layer. Small stellate neurons. • Internal pyramidal layer. Medium to large pyramidal cells. • Fusiform layer. Spindle shaped neurons. Adjacent white matter. The prominence of each layer varies depending on the level of section of the cortex. Q. How many layers can you confidently identify on your section? Note: H&E does not highlight neuron processes and synapses. However, neuron cell bodies and nuclei do stain well in these sections. 20 D:\726851291.doc Another example STUDENT EXPERIMENT 2 MAYFARM BEEF HERD Matching nutrient requirements and feed available for a hypothetical herd of beef cattle to be run on Mayfarm, The University of Sydney. How can we determine how many cattle can be supported by any particular area of land ? To do this we need to know the type of animal – species, age, weight and sex and the nutrient requirements of that class of animal (specifically and primarily energy and protein requirements – more on this later). We then need to assess the productivity of the piece of land in question. We can do this ‘first hand’ by collecting pasture samples and analysing them for energy and protein content or we can estimate the ‘carrying capacity’ of the land using information available from a variety of sources. In the following exercise we will do both. Carrying capacity The productivity of any piece of land can be described in terms of its ‘carrying capacity’ i.e how many animals it can support. We can measure this using the ‘dry sheep equivalent’ system. A dry sheep is defined as a 2 year old wether weighing 45 kg, maintaining weight (i.e not gaining or losing weight) and obviously not lactating or pregnant. Thus, land which is rated at 1 DSE/ha can carry one 45 kg sheep fitting the conditions above, all year round on one hectare of that land. Animals requiring more energy have a higher DSE rating and animals requiring less energy have a lower rating. For example, a 450 kg cow in the last month of pregnancy requires about 6.5 times as much energy as a dry sheep and thus has a DSE of 6.5. (N.B Caution – many people/articles still speak in DSE per acre – the conversion factor is 2.47 acres to 1 hectare or 1 acre is 0.405 hectares). Q: What is an approximate carrying capacity (DSE/ha) for the following categories of land ? (a) native pasture ? ……………………………………………………………………… (b) improved pasture ? …………………………………………………………………... (c) irrigated pasture ? …………………………………………………………………... Where did you find this information ? D:\726851291.doc 21 Another example PROXIMATE ANALYSIS OF FOODS DRY MATTER 1. Take two (2) dry matter tins and note the numbers stamped on them. (Do not write on the tins). 2. Accurately weigh to three decimal places, approximately 5-10 g of sample into a dry matter tin (do not fill more than three-quarters). 3. Place lids beneath the tins and place tins onto a tray. Dry for 24, hours in the oven at 100-105C. 4. Remove the tray of tins from the oven, replace lids using gloves or tongs and allow to cool in a desiccator. 5. Weigh tins again to 3 decimal places. The loss in weight is reported as moisture. 6. Calculate % dry matter of the sample to one decimal place. Results: Pot number Wt. pot + sample Wt. Pot Wt. Sample Wt. pot + dry matter Wt. dry matter % dry matter Mean % dry matter Question: 22 D:\726851291.doc 1. For accurate work the empty tins are dried at 100in a desiccator and then handled with metal tongs during the weighing procedure and when returned to the oven. Why is this? D:\726851291.doc 23 TUTORIALS For example RUMEN PATHOPHYSIOLOGY TUTORIAL Students are expected to discuss their answers to the following questions, answers must be prepared before the class. Consult the reference books listed at the end of the tutorial questions. You have been called to a dairy farm. The farmer has reported that many cows have become ill overnight, and two are dead. On arrival at the farm, you are presented with six recumbent cows. Some cows seem unaffected and are eating form troughs it he paddock. You find bread in the troughs, and the farmer says that he thought it would be good idea to feed stale bread to the cows. Clinically, you find that the recumbent cows are depressed and dehydrated, They also have tachycardia and are tachypnoeic. The cows are grinding their teeth and have diarrhoea. Your diagnosis is acute rumen overload. Questions 24 1. Which metabolite(s) has accumulated in the rumen and is an important contributor to the clinical signs? 2. Would you ever find this metabolite in the ruminal fluid of a clinically normal ruminant? 3. Why are the animals clinically dehydrated (with sunken eyes and inelastic skin)? Why do they have tachycardia and tachypnoea? 4. What laboratory tests would you undertake to assess the degree of dehydration? 5. Consider the likely arterial blood acid-base status of the recumbent cows (pHa, bicarbonate concentration and PaCO2). 6. Why do the animals appear depressed and why do some of the animals that are still walking appear to be weak and/or blind? D:\726851291.doc 3 3.1 READINGS READING 1 - TOPIC The place for inclusion of journal articles/reviews peculiar to the course (to be used for tutorials, etc) 3.2 CASE HISTORY 1 - TOPIC Place for inclusion of case histories 3.3 LECTURE NOTES - TOPIC Place for inclusion of lecture notes. The following headings may be useful: LEARNING OUTCOMES 1. Outcome 1 2. Outome 2 3. etc. R e c o m m e n d e d r e a d i n g Martin et al. (1987) pages 3-9 Smith (1995) pages 1-11 LECTURE OUTLINE Heading 1 Heading 2 D:\726851291.doc 25 4 APPENDIX Space for miscellaneous material that does not ‘fit’ anywhere else. For example, statistical tables, clinical reference data such as normal ranges, past examination papers, data sheets for sample collection in pathology for example. 26 D:\726851291.doc