LANCASHIRE FOG/MANCHESTER FLG/PRESTON PHL CO-ORDINATED SURVEY (JULY – SEPTEMBER 2000) MICROBIOLOGICAL EXAMINATION OF TIRAMISU Protocol – Survey 004010 Objectives 1. To determine the incidence of the use of shell egg in the manufacture of Tiramisu. 2. To determine the microbiological quality of Tiramisu. 3. To compare the microbiological quality of Tiramisu made with shell egg and pasteurised egg. 4. To assess the storage conditions of shell eggs used in the production of Tiramisu. Definitions Tiramisu – cold, coffee and alcohol flavoured dessert. Sampling details 1. The premises should be chosen at random by the participating authority and should preferably include restaurants, hotels, catering establishments and confectioners. N.B. Supermarkets can be used on one sampling occasion only. 2. The time of sampling should be during business opening hours. The participating authority may wish to target certain premises based on local knowledge. Samples may be obtained from the same premises at a later date if there is any difficulty in obtaining the required number of samples. (The offer of payment for the sample is therefore suggested). Any repeat samples should, where possible, be at premises who produce their own Tiramisu. 3. The sampling officer should introduce him/herself and should explain that routine sampling is being carried out and request co-operation in completing a short questionnaire. 4. Unless prepacked the sampling officer should use the premises own serving utensils to transfer the sample into a honey jar. The jar must be at least two thirds full (a sample weight of approximately 200 grams is required). The jar must be placed in a food grade sampling bag sealed, labelled with the authority’s reference number, place of sampling, date and survey number. The sample must then be placed immediately into the cool box. (Pilotsurv004010) Page 1 of 4 5. The sampling officer will then request assistance with the questionnaire and ensure that the questionnaire is completed. A sampling officers guide for completion of the questionnaire is available on page 3. N.B. If any information is not available at the time of the visit the sampling officer must obtain this information at a later date and telephone the details to Preston Public Health Laboratory (Food and Environmental Laboratory) within 7 days. PHLS will follow up prepacked supermarket samples related to type of egg if information is unavailable. 6. Samples obtained in the evening should be handled in accordance with the ‘Procedure for transportation and submission of samples to PHL for microbiological examination’. Alternatively the samples may be stored overnight in a monitored refrigerator before submission to PHL. Survey Details Two premises will be selected on three sampling occasions by the 10 Greater Manchester and the 16 Lancashire/South Cumbria Authorities. If all authorities participate there is a potential of 156 samples. Samples may be submitted Monday – Thursday. Microbiological Test Parameters Aerobic colony count cfu/g Bacillus cereus and other Bacillus spp cfu/g Enterobacteriaceae cfu/g Listeria spp cfu/g E. coli cfu/g Salmonella spp presence/absence 25g Staphylococcus aureus cfu/g The results will be interpreted using category 5 of the PHLS guidelines for ready to eat foods, i.e. the ACC will not be interpreted due to liquid cheese content but may prove useful for other survey purposes. Enquiries The following contacts are available to answer problems or queries related to this survey: Liz Sarker Steve Hoyle Kevin Williamson & Trudi Allen (Pilotsurv004010) - Page 2 of 4 01253 478323 0161 2536198 01772 710115 Information relating to the Questionnaire 1. Type of Premises? Italian restaurant – small local premises as well as national and regional chains where the menu consists of Italian food. Hotel restaurant – self-explanatory. Other restaurant – any premises not included as Italian or hotel. Confectioners – small independent type retailer. Catering establishment such as a café or public house. 2. Premises Risk Rating? This is the most recent scoring given in accordance with Code of Practice 9. 3. Have any staff undergone any food hygiene training in the last 3 years? Training means on a recognised training course (CIEH basic Food Hygiene or above, RIPHH, RSH, etc.). For which you can see documentary evidence such as a certificate. 4. Does the Tiramisu contain any of the following? Tiramisu may include any combination of the products listed. In the case of a product not made on the premises check off the ingredients list of the product. PHLS will follow up prepacked supermarket samples related to type of egg if information not available. 5. Tiramisu should be sampled from the point of retail i.e. where the customer gets it from. 6. Was the Tiramisu made on the premises? Self-explanatory. 7. How long since the Tiramisu was produced? If this information can be obtained at a later date – do not use the ‘not known’ box. Forward details to PHL within 7 days. 8. If shell eggs are used how are they stored? Self-explanatory but sampling officers may request to see storage conditions. (Pilotsurv004010) Page 3 of 4 9. Is the Tiramisu within its use by date? Self-explanatory, but if ‘no’ or ‘not known’ this may be passed to the appropriate EHO for information. 10. Who is the supplier of the Tiramisu? Self-explanatory but address details would be useful rather than a name only. Supermarket samples must be completed with as much information as possible including product name and brand name. 11. Self-explanatory. (Pilotsurv004010) Page 4 of 4