CHAPTER IV
RESULTS AND DISCUSSION
The main problem of the study of Hermit Crabs at different bleach
concentrations is to determine the Hermit Crabs’ mortality rates at different bleach
concentrations and verify if there is a significant difference among the mortality rates
of the Hermit Crabs. The whole course of experimentation for this study was
conducted on January 5, 2011 until January 9, 2011 at Initao, Misamis Oriental. The
observation of the mortality rate of the Hermit Crabs stopped on the fourth day as
LC50 had been reached or 50% of the Hermit Crabs of set-up E with 10,000 ppm, died.
A. Results of Experimentation
Table 4.1 Hermit Crabs' Mortality Rate
Set-up
A
B
C
D
E
Bleach Concentration (ppm)
0
10
100
1,000
10,000
# of Dead Hermit Crabs
0
1
1
3
5
Mortality Rate
0%
10%
10%
30%
50%
Table 4.1 shows the Hermit Crabs’ Mortality Rate at different bleach
concentrations. The observation for their mortality rate stopped on the fourth day
when 5 Hermit Crabs of set-up E, 10,000 ppm, died. This is also known as LC50. At
LC50, it was observed that no Hermit Crab died on 0 ppm, 1 Hermit Crab on both 10
and 100 ppm and 3 Hermit Crabs on 1,000 ppm. With these data, the researchers
calculated for the Hermit Crabs’ mortality rate. Set-up A with 0%, B and C with 10%,
D with 30% and E with 50% mortality.
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To verify if there is a significant difference on the mortality rate of Hermit
Crabs at different bleach concentrations, Analysis of Variance (ANOVA) in the
Megastat add-in from Microsoft Excel was conducted which yield to the following
results.
Table 4.2 ANOVA Table for Hermit Crabs' Mortality Rate
Mean
n
Std. Dev
Bleach
1112
2,222.0
5
4,368.17 Concentration
1112
2.0
5
2.00 Dead Hermit Crabs
1,112.0 10
3,138.38 Total
Source
SS
Treatment
12,321,000.00
Error
76,323,696.00
Total
88,644,696.00
*significant at 0.05 level
df
1
8
9
MS
12,321,000.000
9,540,462.000
F
1.29
pvalue
.2887
Table 4.2 shows the ANOVA Table for Hermit Crabs' Mortality Rate at
different bleach concentrations. Since the F-statistics computed in the ANOVA table
is less than the F-table statistics or the P-value is lesser than the alpha level of
significance which is 0.05, then the null hypothesis is rejected. That is, there is a
significant difference on the mortality rate of Hermit Crabs at different bleach
concentrations.